| 质粒名称: | pAAVLINK-EFS-HA-5'-CACNA1A |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001691 |
| 产品用途: | 5' AAVLINK plasmid for CACNA1A expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EFS |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CACNA1A |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EFS-HA-5'-CACNA1A
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EFS-HA-5'-CACNA1A载体质粒基本信息
pAAVLINK-EFS-HA-5'-CACNA1A质粒图谱载体图谱和pAAVLINK-EFS-HA-5'-CACNA1A载体序列质粒序列多克隆位点信息
pAAVLINK-EFS-HA-5'-CACNA1A质粒载体简介
pAAVLINK-EFS-HA-5'-CACNA1A质粒序列载体序列
LOCUS pAAVLINK-EFS-HA-5'-CACNA1A 7175 bp DNA circular SYN 20-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7175)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 2, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7175
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..356
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
CDS 371..397
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 404..4315
/codon_start=1
/label=CACNA1A
/translation="MARFGDEMPARYGGGGSGAAAGVVVGSGGGRGAGGSRQGGQPGAQ
RMYKQSMAQRARTMALYNPIPVRQNCLTVNRSLFLFSEDNVVRKYAKKITEWPPFEYMI
LATIIANCIVLALEQHLPDDDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFAFHKGS
YLRNGWNVMDFVVVLTGILATVGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKA
MIPLLQIGLLLFFAILIFAIIGLEFYMGKFHTTCFEEGTDDIQGESPAPCGTEEPARTC
PNGTKCQPYWEGPNNGITQFDNILFAVLTVFQCITMEGWTDLLYNSNDASGNTWNWLYF
IPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYMEWISKA
EEVILAEDETDGEQRHPFDALRRTTIKKSKTDLLNPEEAEDQLADIASVGSPFARASIK
SAKLENSTFFHKKERRMRFYIRRMVKTQAFYWTVLSLVALNTLCVAIVHYNQPEWLSDF
LYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCGVIIGSIFEVIWAVIKPGTSF
GISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLF
GGQFNFDEGTPPTNFDTFPAAIMTVFQILTGEDWNEVMYDGIKSQGGVQGGMVFSIYFI
VLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLALQKAKEVAEVSPLSA
ANMSIAVKEQQKNQKPAKSVWEQRTSEMRKQNLLASREALYNEMDPDERWKAAYTRHLR
PDMKTHLDRPLVVDPQENRNNNTNKSRAAEPTVDQRLGQQRAEDFLRKQARYHDRARDP
SGSAGLDARRPWAGSQEAELSREGPYGRESDHHAREGSLEQPGFWEGEAERGKAGDPHR
RHVHRQGGSRESRSGSPRTGADGEHRRHRAHRRPGEEGPEDKAERRARHREGSRPARGG
EGEGEGPDGGERRRRHRHGAPATYEGDARREDKERRHRRRKENQGSGVPVSGPNLSTTR
PIQQDLGRQDPPLAEDIDNMKNNKLATAESAAPHGSLGHAGLPQSPAKMGNSTDPGPML
AIPAMATNPQNAASRRTPNNPGNPSNPGPPKTPENSLIVTNPSGTQTNSAKTARKPDHT
TVDIPPACPPPLNHTVVQVNKNANPDPLPKKEEEKKEEEEDDRGEDGPKPMPPYSSMFI
LSTTNPLRRLCHYILNLRYFEMCILMVIAMSSIALAAEDPVQPNAPRNNVLRYFDYVFT
GVFTFEMVIKMIDLGLVLHQ"
misc_feature 445..450
/label=SDS
misc_feature 1093..1098
/label=SDS
misc_feature 1096..1101
/label=SDS
misc_feature 1820..1825
/label=SDS
misc_feature 2512..2517
/label=SDS
misc_feature 2631..2636
/label=SDS
misc_feature 3063..3068
/label=SDS
misc_feature 3300..3305
/label=SDS
misc_feature 3485..3490
/label=SDS
misc_feature 4008..4013
/label=SDS
misc_feature 4011..4016
/label=SDS
misc_feature 4014..4019
/label=SDS
intron 4316..4356
/label=Splicing Donor
misc_feature 4360..4393
/label=lox2272-JT1571
repeat_region 4438..4578
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4438..4567
/label=AAV2 ITR
rep_origin 4653..5108
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5390..5494
/gene="bla"
/label=AmpR promoter
CDS 5495..6355
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6526..7114
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgggcag agcgcacatc gcccacagtc cccgagaagt
181 tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg
241 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
301 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagcgta
361 cgccaccatg tacccttatg acgtacctga ctatgctgga tctatggccc gcttcggaga
421 cgagatgccg gcccgctacg ggggaggagg ctccggggca gccgccgggg tggtcgtggg
481 cagcggaggc gggcgaggag ccgggggcag ccggcagggc gggcagcccg gggcgcaaag
541 gatgtacaag cagtcaatgg cgcagagagc gcggaccatg gcactctaca accccatccc
601 cgtccgacag aactgcctca cggttaaccg gtctctcttc ctcttcagcg aagacaacgt
661 ggtgagaaaa tacgccaaaa agatcaccga atggcctccc tttgaatata tgattttagc
721 caccatcata gcgaattgca tcgtcctcgc actggagcag catctgcctg atgatgacaa
781 gaccccgatg tctgaacggc tggatgacac agaaccatac ttcattggaa ttttttgttt
841 cgaggctgga attaaaatca ttgcccttgg gtttgccttc cacaaaggct cctacttgag
901 gaatggctgg aatgtcatgg actttgtggt ggtgctaacg ggcatcttgg cgacagttgg
961 gacggagttt gacctacgga cgctgagggc agttcgagtg ctgcggccgc tcaagctggt
1021 gtctggaatc ccaagtttac aagtcgtcct gaagtcgatc atgaaggcga tgatcccttt
1081 gctgcagatc ggcctcctcc tattttttgc aatccttatt tttgcaatca tagggttaga
1141 attttatatg ggaaaatttc ataccacctg ctttgaagag gggacagatg acattcaggg
1201 tgagtctccg gctccatgtg ggacagaaga gcccgcccgc acctgcccca atgggaccaa
1261 atgtcagccc tactgggaag ggcccaacaa cgggatcact cagttcgaca acatcctgtt
1321 tgcagtgctg actgttttcc agtgcataac catggaaggg tggactgatc tcctctacaa
1381 tagcaacgat gcctcaggga acacttggaa ctggttgtac ttcatccccc tcatcatcat
1441 cggctccttt tttatgctga accttgtgct gggtgtgctg tcaggggagt ttgccaaaga
1501 aagggaacgg gtggagaacc ggcgggcttt tctgaagctg aggcggcaac aacagattga
1561 acgtgagctc aatgggtaca tggagtggat ctcaaaagca gaagaggtga tcctcgccga
1621 ggatgaaact gacggggagc agaggcatcc ctttgatgct ctgcggagaa ccaccataaa
1681 gaaaagcaag acagatttgc tcaaccccga agaggctgag gatcagctgg ctgatatagc
1741 ctctgtgggt tctcccttcg cccgagccag cattaaaagt gccaagctgg agaactcgac
1801 cttttttcac aaaaaggaga ggaggatgcg tttctacatc cgccgcatgg tcaaaactca
1861 ggccttctac tggactgtac tcagtttggt agctctcaac acgctgtgtg ttgctattgt
1921 tcactacaac cagcccgagt ggctctccga cttcctttac tatgcagaat tcattttctt
1981 aggactcttt atgtccgaaa tgtttataaa aatgtacggg cttgggacgc ggccttactt
2041 ccactcttcc ttcaactgct ttgactgtgg ggttatcatt gggagcatct tcgaggtcat
2101 ctgggctgtc ataaaacctg gcacatcctt tggaatcagc gtgttacgag ccctcaggtt
2161 attgcgtatt ttcaaagtca caaagtactg ggcatctctc agaaacctgg tcgtctctct
2221 cctcaactcc atgaagtcca tcatcagcct gttgtttctc cttttcctgt tcattgtcgt
2281 cttcgccctt ttgggaatgc aactcttcgg cggccagttt aatttcgatg aagggactcc
2341 tcccaccaac ttcgatactt ttccagcagc aataatgacg gtgtttcaga tcctgacggg
2401 cgaagactgg aacgaggtca tgtacgacgg gatcaagtct caggggggcg tgcagggcgg
2461 catggtgttc tccatctatt tcattgtact gacgctcttt gggaactaca ccctcctgaa
2521 tgtgttcttg gccatcgctg tggacaatct ggccaacgcc caggagctca ccaaggacga
2581 gcaagaggaa gaagaagcag cgaaccagaa acttgcccta cagaaagcca aggaggtggc
2641 agaagtgagt cctctgtccg cggccaacat gtctatagct gtgaaagagc aacagaagaa
2701 tcaaaagcca gccaagtccg tgtgggagca gcggaccagt gagatgcgaa agcagaactt
2761 gctggccagc cgggaggccc tgtataacga aatggacccg gacgagcgct ggaaggctgc
2821 ctacacgcgg cacctgcggc cagacatgaa gacgcacttg gaccggccgc tggtggtgga
2881 cccgcaggag aaccgcaaca acaacaccaa caagagccgg gcggccgagc ccaccgtgga
2941 ccagcgcctc ggccagcagc gcgccgagga cttcctcagg aaacaggccc gctaccacga
3001 tcgggcccgg gaccccagcg gctcggcggg cctggacgca cggaggccct gggcgggaag
3061 ccaggaggcc gagctgagcc gggagggacc ctacggccgc gagtcggacc accacgcccg
3121 ggagggcagc ctggagcaac ccgggttctg ggagggcgag gccgagcgag gcaaggccgg
3181 ggacccccac cggaggcacg tgcaccggca ggggggcagc agggagagcc gcagcgggtc
3241 cccgcgcacg ggcgcggacg gggagcatcg acgtcatcgc gcgcaccgca ggcccgggga
3301 ggagggtccg gaggacaagg cggagcggag ggcgcggcac cgcgagggca gccggccggc
3361 ccggggcggc gagggcgagg gcgagggccc cgacgggggc gagcgcagga gaaggcaccg
3421 gcatggcgct ccagccacgt acgaggggga cgcgcggagg gaggacaagg agcggaggca
3481 tcggaggagg aaagagaacc agggctccgg ggtccctgtg tcgggcccca acctgtcaac
3541 cacccggcca atccagcagg acctgggccg ccaagaccca cccctggcag aggatattga
3601 caacatgaag aacaacaagc tggccaccgc ggagtcggcc gctccccacg gcagccttgg
3661 ccacgccggc ctgccccaga gcccagccaa gatgggaaac agcaccgacc ccggccccat
3721 gctggccatc cctgccatgg ccaccaaccc ccagaacgcc gccagccgcc ggacgcccaa
3781 caacccgggg aacccatcca atcccggccc ccccaagacc cccgagaata gccttatcgt
3841 caccaacccc agcggcaccc agaccaattc agctaagact gccaggaaac ccgaccacac
3901 cacagtggac atccccccag cctgcccacc ccccctcaac cacaccgtcg tacaagtgaa
3961 caaaaacgcc aacccagacc cactgccaaa aaaagaggaa gagaagaagg aggaggagga
4021 agacgaccgt ggggaagacg gccctaagcc aatgcctccc tatagctcca tgttcatcct
4081 gtccacgacc aacccccttc gccgcctgtg ccattacatc ctgaacctgc gctactttga
4141 gatgtgcatc ctcatggtca ttgccatgag cagcatcgcc ctggccgccg aggaccctgt
4201 gcagcccaac gcacctcgga acaacgtgct gcgatacttt gactacgttt ttacaggcgt
4261 ctttaccttt gagatggtga tcaagatgat tgacctgggg ctcgtcctgc atcaggtaag
4321 tatcaaggtt acaagacagg tttaaggaga ccaataagct attattcgta taggatactt
4381 tatacgaagt tattaagtta tggagaccgg tgtggcacgt gcggaccgag cggccgcagg
4441 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
4501 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
4561 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
4621 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
4681 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc
4741 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
4801 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
4861 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
4921 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
4981 caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg
5041 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
5101 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
5161 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
5221 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
5281 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
5341 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
5401 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
5461 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
5521 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
5581 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
5641 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
5701 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
5761 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
5821 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
5881 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
5941 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
6001 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
6061 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
6121 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
6181 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
6241 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
6301 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
6361 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
6421 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
6481 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
6541 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
6601 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
6661 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
6721 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
6781 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
6841 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
6901 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
6961 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
7021 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
7081 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
7141 gttcctggcc ttttgctggc cttttgctca catgt
//
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