LOCUS pLenti-DsRed_IRES_SNCA:EGFP 10354 bp DNA circular SYN 22-MAY-2021
DEFINITION Lentiviral overexpression vector to make stable bicistronic cell line for alpha-Syn screen.
SOURCE synthetic DNA construct.
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10354)
AUTHORS Rousseaux MW, de Haro M, Lasagna-Reeves CA, De Maio A, Park J,
Jafar-Nejad P, Al-Ramahi I, Sharma A, See L, Lu N, Vilanova-Velez L,
Klisch TJ, Westbrook TF, Troncoso JC, Botas J, Zoghbi HY
TITLE TRIM28 regulates the nuclear accumulation and toxicity of both
alpha-synuclein and tau.
JOURNAL Elife. 2016 Oct 25;5. pii: e19809. doi: 10.7554/eLife.19809.
REFERENCE 2 (bases 1 to 10354)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Tuesday, Oct 10, 2023 from SnapGene 4.3.6 to Vector NTI(R)
format
https://www.biofeng.com
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/
COMMENT ORIGDB|GenBank
COMMENT VNTDATE|950227200|
COMMENT VNTDBDATE|950227200|
COMMENT LSOWNER|
COMMENT VNTNAME|pLenti-DsRed_IRES_SNCA:EGFP|
FEATURES Location/Qualifiers
LTR 376..633
/vntifkey="19"
/label=5'\LTR\(truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 680..805
/vntifkey="21"
/label=HIV-1\Psi
/note="packaging signal of human immunodeficiency virus type 1"
misc_feature 1302..1535
/vntifkey="21"
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm."
CDS 1720..1764
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/vntifkey="4"
/label=gp41\peptide
/note="recognized by the 2H10 single-chain llama nanobody"
misc_feature 2057..2174
/vntifkey="21"
/label=cPPT/CTS
/note="central polypurine tract and central termination sequence of HIV-1"
enhancer 2226..2529
/vntifkey="9"
/label=CMV\enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2530..2733
/vntifkey="30"
/label=CMV\promoter
/note="human cytomegalovirus (CMV) immediate early promoter"
primer 2684..2704
/vntifkey="27"
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer sequence: CGCAAATGGGCGGTAGGCGTG"
primer 2730..2754
/vntifkey="27"
/label=LNCX
/note="Human CMV promoter, forward primer sequence: AGCTCGTTTAGTGAACCGTCAGATC"
regulatory 2837..2846
/regulatory_class="other"
/vntifkey="21"
/label=Kozak\sequence
/note="Unknown feature type:regulatory vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
CDS 2843..3520
/codon_start=1
/product="rapidly maturing tetrameric variant of DsRed
fluorescent protein (Bevis and Glick, 2002)"
/vntifkey="4"
/label=DsRed-Express
/note="mammalian codon-optimized"
primer complement(2973..2991)
/vntifkey="27"
/label=mCherry-R
/note="mCherry, reverse primer sequence: TTGGTCACCTTCAGCTTGG"
primer complement(3023..3043)
/vntifkey="27"
/label=DsRed1-N
/note="DsRed1, reverse primer sequence: GTACTGGAACTGGGGGGACAG"
primer 3241..3260
/vntifkey="27"
/label=mCherry-F
/note="mCherry, forward primer sequence: CCCCGTAATGCAGAAGAAGA"
primer 3435..3458
/vntifkey="27"
/label=DsRed1-C
/note="DsRed1, forward primer sequence: AGCTGGACATCACCTCCCACAACG"
misc_feature 3546..4119
/vntifkey="21"
/label=IRES
/note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)"
misc_feature 3569..4121
/vntifkey="21"
/label=IRES
/note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)"
primer complement(3713..3730)
/vntifkey="27"
/label=IRES\reverse
/note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev sequence: GCATTCCTTTGGCGAGAG"
primer 3940..3959
/vntifkey="27"
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer sequence: TGGCTCTCCTCAAGCGTATT"
CDS 4571..5290
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/vntifkey="4"
/label=EGFP
/note="mammalian codon-optimized"
CDS 4571..5287
/codon_start=1
/product="enhanced GFP"
/vntifkey="4"
/label=EGFP
primer complement(4616..4637)
/vntifkey="27"
/label=EGFP-N
/note="EGFP, reverse primer sequence: CGTCGCCGTCCAGCTCGACCAG"
primer complement(4877..4896)
/vntifkey="27"
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer sequence: GTCTTGTAGTTGCCGTCGTC"
primer 5224..5245
/vntifkey="27"
/label=EGFP-C
/note="EGFP, forward primer sequence: CATGGTCCTGCTGGAGTTCGTG"
primer complement(5310..5329)
/vntifkey="27"
/label=T7
/note="T7 promoter, forward primer sequence: TAATACGACTCACTATAGGG"
promoter complement(5311..5329)
/vntifkey="30"
/label=T7\promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer complement(5374..5397)
/vntifkey="27"
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer sequence: CAGCGGGGCTGCTAAAGCGCATGC"
primer 5747..5766
/vntifkey="27"
/label=mPGK-F
/note="Mouse PGK promoter, forward primer sequence: CATTCTGCACGCTTCAAAAG"
CDS 5835..6629
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/vntifkey="4"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))"
primer complement(5889..5908)
/vntifkey="27"
/label=Neo-R
/note="Neomycin resistance gene, reverse primer sequence: GCCCAGTCATAGCCGAATAG"
primer 6499..6518
/vntifkey="27"
/label=Neo-F
/note="Neomycin resistance gene, forward primer sequence: CGTTGGCTACCCGTGATATT"
misc_feature 6743..7331
/vntifkey="21"
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional regulatory element"
primer complement(6796..6816)
/vntifkey="27"
/label=WPRE-R
/note="WPRE, reverse primer sequence: CATAGCGTAAAAGGAGCAACA"
CDS complement(7214..7225)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/vntifkey="4"
/label=Factor\Xa\site
LTR 7406..7639
/vntifkey="19"
/label=3'\LTR\(Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from HIV-1"
primer complement(7758..7776)
/vntifkey="27"
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward primer sequence: AATAGGCGTATCACGAGGC"
promoter 7844..7948
/gene="bla"
/vntifkey="30"
/label=AmpR\promoter
CDS 7949..8809
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/vntifkey="4"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
primer complement(8167..8186)
/vntifkey="27"
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer sequence: ATAATACCGCGCCACATAGC"
rep_origin 8980..9568
/direction=RIGHT
/vntifkey="33"
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
primer 9469..9488
/vntifkey="27"
/label=pBR322ori-F
/note="pBR322 origin, forward primer sequence: GGGAAACGCCTGGTATCTTT"
primer 9722..9739
/vntifkey="27"
/label=L4440
/note="L4440 vector, forward primer sequence: AGCGAGTCAGTGAGCGAG"
primer 9831..9850
/vntifkey="27"
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer sequence: TATTTATGCAGAGGCCGAGG"
protein_bind 9979..10000
/bound_moiety="E. coli catabolite activator protein"
/vntifkey="31"
/label=CAP\binding\site
/note="CAP binding activates transcription in the presence of cAMP."
promoter 10015..10045
/vntifkey="30"
/label=lac\promoter
/note="promoter for the E. coli lac operon"
protein_bind 10053..10069
/bound_moiety="lac repressor encoded by lacI"
/vntifkey="31"
/label=lac\operator
/note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer 10058..10080
/vntifkey="27"
/label=M13/pUC\Reverse
/note="In lacZ gene sequence: AGCGGATAACAATTTCACACAGG"
primer_bind 10077..10093
/vntifkey="28"
/label=M13\rev
/note="common sequencing primer, one of multiple similar variants"
primer 10077..10093
/vntifkey="27"
/label=M13\Reverse
/note="In lacZ gene. Also called M13-rev sequence: CAGGAAACAGCTATGAC"
primer 10143..10162
/vntifkey="27"
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer sequence: GTGGTTTGTCCAAACTCATC"
BASE COUNT 2584 a 2631 c 2759 g 2380 t
ORIGIN
1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccgaat tcacaaatgg
2041 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
2101 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
2161 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg actagtggag
2221 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
2281 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
2341 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
2401 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
2461 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
2521 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
2581 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
2641 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
2701 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
2761 agacgccatc cacgctgttt tgacctccat agaagacacc ggcggcctag cgctaccgga
2821 ctcagatcca ccggtcgcca ccatggcctc ctccgaggac gtcatcaagg agttcatgcg
2881 cttcaaggtg cgcatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
2941 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc
3001 cctgcccttc gcctgggaca tcctgtcccc ccagttccag tacggctcca aggtgtacgt
3061 gaagcacccc gccgacatcc ccgactacaa gaagctgtcc ttccccgagg gcttcaagtg
3121 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
3181 gcaggacggc tccttcatct acaaggtgaa gttcatcggc gtgaacttcc cctccgacgg
3241 ccccgtaatg cagaagaaga ctatgggctg ggaggcctcc accgagcgcc tgtacccccg
3301 cgacggcgtg ctgaagggcg agatccacaa ggccctgaag ctgaaggacg gcggccacta
3361 cctggtggag ttcaagtcca tctacatggc caagaagccc gtgcagctgc ccggctacta
3421 ctacgtggac tccaagctgg acatcacctc ccacaacgag gactacacca tcgtggagca
3481 gtacgagcgc gccgagggcc gccaccacct gttcctgtag cggccaattc tgcagtcgac
3541 atccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
3601 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
3661 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
3721 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
3781 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
3841 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
3901 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
3961 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
4021 cggtacacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
4081 cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa tcgatatgga
4141 tgtattcatg aaaggacttt caaaggccaa ggagggagtt gtggctgctg ctgagaaaac
4201 caaacagggt gtggcagaag cagcaggaaa gacaaaagag ggtgttctct atgtaggctc
4261 caaaaccaag gagggagtgg tgcatggtgt ggcaacagtg gctgagaaga ccaaagagca
4321 agtgacaaat gttggaggag cagtggtgac gggtgtgaca gcagtagccc agaagacagt
4381 ggagggagca gggagcattg cagcagccac tggctttgtc aaaaaggacc agttgggcaa
4441 gaatgaagaa ggagccccac aggaaggaat tctggaagat atgcctgtgg atcctgacaa
4501 tgaggcttat gaaatgcctt ctgaggaagg gtatcaagac tacgaacctg aagccctcga
4561 ggcggccgct atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
4621 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
4681 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
4741 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
4801 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
4861 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
4921 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
4981 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
5041 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
5101 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
5161 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
5221 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
5281 gtacaagtaa tctagataac tgatcctctc cctatagtga gtcgtattaa attctaccgg
5341 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc
5401 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc
5461 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg
5521 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt
5581 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg
5641 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt
5701 gggtccgggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
5761 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
5821 agcccaagct taccatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
5881 tggagaggct attcggctat gactgggcac aacagacgat cggctgctct gatgccgccg
5941 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
6001 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
6061 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
6121 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
6181 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
6241 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
6301 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
6361 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
6421 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
6481 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
6541 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
6601 tctatcgcct tcttgacgag ttcttctgac ctgaggatca taatcagcca taccacattt
6661 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
6721 atgaatgcaa ttgatagatc ctaatcaacc tctggattac aaaatttgtg aaagattgac
6781 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
6841 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
6901 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
6961 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
7021 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
7081 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
7141 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
7201 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
7261 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
7321 cgcctccccg cctgagatcc tttaagacca atgacttaca aggcagctgt agatcttagc
7381 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
7441 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
7501 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
7561 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
7621 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
7681 gcaaagaaat gaatatcaga gagtgagagg cccgggttaa ttaaggaaag ggctagatca
7741 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
7801 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
7861 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
7921 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
7981 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
8041 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
8101 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
8161 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
8221 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
8281 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
8341 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
8401 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
8461 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
8521 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
8581 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
8641 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
8701 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
8761 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
8821 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
8881 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
8941 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
9001 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
9061 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
9121 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
9181 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
9241 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
9301 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
9361 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
9421 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
9481 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
9541 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
9601 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
9661 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
9721 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
9781 gcgttggccg attcattaat gcagcaagct catggctgac taattttttt tatttatgca
9841 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
9901 ggcctaggct tttgcaaaaa gctccccgtg gcacgacagg tttcccgact ggaaagcggg
9961 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
10021 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
10081 aaacagctat gacatgatta cgaatttcac aaataaagca tttttttcac tgcattctag
10141 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca
10201 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct
10261 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg
10321 tatttgttaa atatgtacta caaacttagt agtt
//
