pAAV-CMV-iRFP

价格:1100元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Addgene
载体名称: pAAV-CMV-iRFP
质粒类型: 腺病毒载体;荧光蛋白报告载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 5603 bp
5' 测序引物及序列: pCAX-F (CAGCTCCTGGGCAACGTGC)
3' 测序引物及序列: C-CMV-24 (TATTAGGACAAGGCTGGTGGGCAC)
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: 红外荧光蛋白iRFP
克隆菌株: Stbl3 或 NEB Stble
宿主细胞(系): 常规哺乳动物细胞系
备注: --
产品目录号: #64887
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 腺病毒

载体质粒图谱和多克隆位点信息

pAAV-CMV-iRFP载体图谱



载体简介

载体序列

LOCUS       pAAV-CMV-iRFP Sequencing Result	5603 bp 	DNA	SYN
DEFINITION  pAAV-CMV-iRFP Sequencing Result
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTNAME|pAAV-CMV-iRFP|
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="pAAV-CMV-iRFP Sequencing Result"
                     /mol_type="other DNA"
     promoter        159..710
                     /label="CMV_immearly_promoter"
     misc_feature    214..501
                     /label="CAG_enhancer"
     misc_feature    667..687
                     /label="CMV_fwd_primer"
     promoter        668..737
                     /label="CMV_promoter"
     promoter        680..799
                     /label="CMV2_promoter"
     misc_feature    711..730
                     /label="pCEP_fwd_primer"
     misc_feature    713..737
                     /label="LNCX_primer"
     misc_feature    809..1301
                     /label="bGlob_int"
     CDS             1341..2291
                     /label="ORF frame 3"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAG
                     SDNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAG
                     FIGSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVR
                     KITGFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRI
                     IPDINYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGE
                     RLWGLIVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE*"
     terminator      2348..2826
                     /label="hGH_PA_terminator"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAG
                     SDNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAG
                     FIGSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVR
                     KITGFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRI
                     IPDINYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGE
                     RLWGLIVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE*"
     rep_origin      3098..3404
                     /label="f1_origin"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAG
                     SDNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAG
                     FIGSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVR
                     KITGFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRI
                     IPDINYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGE
                     RLWGLIVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE*"
     misc_feature    3672..3694
                     /label="pGEX_3_primer"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAG
                     SDNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAG
                     FIGSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVR
                     KITGFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRI
                     IPDINYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGE
                     RLWGLIVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE*"
     promoter        3853..3881
                     /label="AmpR_promoter"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAG
                     SDNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAG
                     FIGSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVR
                     KITGFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRI
                     IPDINYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGE
                     RLWGLIVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE*"
     gene            3923..4783
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAG
                     SDNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAG
                     FIGSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVR
                     KITGFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRI
                     IPDINYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGE
                     RLWGLIVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE*"
     CDS             3923..4783
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      4938..5557
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT
   61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT
  121 AGGGGTTCCT GCGGCCGCAC GCGTGGAGCT AGTTATTAAT AGTAATCAAT TACGGGGTCA
  181 TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT
  241 GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA
  301 ACGTCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC
  361 TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT
  421 AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG
  481 TACATCTACG TATTAGTCAT CGCTATTACC ATGGTGATGC GGTTTTGGCA GTACATCAAT
  541 GGGCGTGGAT AGCGGTTTGA CTCACGGGGA TTTCCAAGTC TCCACCCCAT TGACGTCAAT
  601 GGGAGTTTGT TTTGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAC AACTCCGCCC
  661 CATTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT
  721 TAGTGAACCG TCAGATCGCC TGGAGACGCC ATCCACGCTG TTTTGACCTC CATAGAAGAC
  781 ACCGGGACCG ATCCAGCCTC CGCGGATTCG AATCCCGGCC GGGAACGGTG CATTGGAACG
  841 CGGATTCCCC GTGCCAAGAG TGACGTAAGT ACCGCCTATA GAGTCTATAG GCCCACAAAA
  901 AATGCTTTCT TCTTTTAATA TACTTTTTTG TTTATCTTAT TTCTAATACT TTCCCTAATC
  961 TCTTTCTTTC AGGGCAATAA TGATACAATG TATCATGCCT CTTTGCACCA TTCTAAAGAA
 1021 TAACAGTGAT AATTTCTGGG TTAAGGCAAT AGCAATATTT CTGCATATAA ATATTTCTGC
 1081 ATATAAATTG TAACTGATGT AAGAGGTTTC ATATTGCTAA TAGCAGCTAC AATCCAGCTA
 1141 CCATTCTGCT TTTATTTTAT GGTTGGGATA AGGCTGGATT ATTCTGAGTC CAAGCTAGGC
 1201 CCTTTTGCTA ATCATGTTCA TACCTCTTAT CTTCCTCCCA CAGCTCCTGG GCAACGTGCT
 1261 GGTCTGTGTG CTGGCCCATC ACTTTGGCAA AGAATTGGGA TTCGAACATC GATTGAATTC
 1321 CCCGGGGATC TGCCGCCACC ATGGCGGAAG GATCCGTCGC CAGGCAGCCT GACCTCTTGA
 1381 CCTGCGACGA TGAGCCGATC CATATCCCCG GTGCCATCCA ACCGCATGGA CTGCTGCTCG
 1441 CCCTCGCCGC CGACATGACG ATCGTTGCCG GCAGCGACAA CCTTCCCGAA CTCACCGGAC
 1501 TGGCGATCGG CGCCCTGATC GGCCGCTCTG CGGCCGATGT CTTCGACTCG GAGACGCACA
 1561 ACCGTCTGAC GATCGCCTTG GCCGAGCCCG GGGCGGCCGT CGGAGCACCG ATCACTGTCG
 1621 GCTtCACGAT GCGAAAGgAC GCAGGCTTCA TCGGCTCCTG GCATCGCCAT GATCAGCTCA
 1681 TCTtccTCGA GCTCGAGCCT CCCCAGCGGG ACGTCgccga gccgcaggcg ttcttccgcc
 1741 gcaCCAACAG CGCCATCCGC CGCCTGCAGG CCGCCGAAAC CTTGGAAAGC GCCTGCGCCG
 1801 CCGCGGCGCA AGAGGTGCGG AAGATTACCG GCTTCGATCG GGTGATGATC TATCGCTTCG
 1861 CCTCCGACTT CAGCGGCGAA GTGATCGCAG AGGATCGGTG CGCCGAGGTC GAGTCAAAAC
 1921 TAGGCCTGCA CTATCCTGCC TCAACCGTGC CGGCGCAGGC CCGTCGGCTC TATACCATCA
 1981 ACCCGGTACG GATCATTCCC GATATCAATT ATCGGCCGGT GCCGGTCACC CCAGACCTCA
 2041 ATCCGGTCAC CGGGCGGCCG ATTGATCTTA GCTTCGCCAT CCTGCGCAGC GTCTCGCCCG
 2101 TCCATCTGGA ATTCATGCGC AACATAGGCA TGCACGGCAC GATGTCGATC TCGATTTTGC
 2161 GCGGCGAGCG ACTGTGGGGA TTGATCGTTT GCCATCACCG AACGCCGTAC TACGTCGATC
 2221 TCGATGGCCG CCAAGCCTGC GAGCTAGTCG CCCAGGTTCT GGCCTGGCAG ATCGGCGTGA
 2281 TGGAAGAGTG AGTCGACGCG GCCGCTCGAG CCTAAGCTTG CCTCGAGCAG CGCTGCTCGA
 2341 GAGATCTACG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT
 2401 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC
 2461 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT
 2521 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA
 2581 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT
 2641 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG
 2701 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC
 2761 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT
 2821 GTCCTTCTGA TTTTGTAGGT AACCACGTGC GGACCGAGCG GCCGCAGGAA CCCCTAGTGA
 2881 TGGAGTTGGC CACTCCCTCT CTGCGCGCTC GCTCGCTCAC TGAGGCCGGG CGACCAAAGG
 2941 TCGCCCGACG CCCGGGCTTT GCCCGGGCGG CCTCAGTGAG CGAGCGAGCG CGCAGCTGCC
 3001 TGCAGGGGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA
 3061 TACGTCAAAG CAACCATAGT ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT
 3121 GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT
 3181 CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT
 3241 CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTTGGG
 3301 TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA
 3361 GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC
 3421 GGGCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA
 3481 GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGTTTA CAATTTTATG
 3541 GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGCCCC GACACCCGCC
 3601 AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC
 3661 TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC
 3721 GAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT
 3781 TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT
 3841 TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA
 3901 ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT
 3961 TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA
 4021 TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA
 4081 GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT
 4141 GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT
 4201 ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA
 4261 TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC
 4321 CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT
 4381 GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA
 4441 CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC
 4501 TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA
 4561 AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC
 4621 TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC
 4681 CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG
 4741 ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA
 4801 CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA
 4861 GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC
 4921 GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT
 4981 CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA
 5041 GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT
 5101 CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA
 5161 CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC
 5221 CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG
 5281 TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG
 5341 TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG
 5401 CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT
 5461 TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC
 5521 AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT
 5581 TTGCTGGCCT TTTGCTCACA TGT
//

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