| 质粒名称: | pS-sg:GFP |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 196296 |
| 存储实验室: | Zhenghe Li |
|
载体骨架基本信息 |
|
| 载体名称: | pCB301-2μ-HDV |
| 完整质粒大小: | 10189 bp |
| 载体类型: | Plant Expression, CRISPR |
| 载体抗性: | Kanamycin |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Full length TSWV S antigenome encoding sgRNA:GFP fusion in place of viral NSs / SpeI-BsaI-BsaI-Scaffold-GFP-SpeI |
| 物种来源: | Synthetic |
| 启动子: | duplicated cauliflower mosaic virus 35S promoter |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | SpeI(未破坏) |
| 3’克隆位点: | SpeI(未破坏) |
| 5’测序引物 | 35S promoter/forward primer: CTATCCTTCGCAAGACCCTTC |
| 3’测序引物 | Nos terminator/reverse primer: TCATCGCAAGACCGGCAA |
|
文献引用方法 |
|
| 材料和方法部分: | pS-sg:GFP was a gift from Zhenghe Li (Addgene plasmid # 196296 ; http://n2t.net/addgene:196296 ; RRID:Addgene_196296) |
| 参考文献部分: | Engineered biocontainable RNA virus vectors for non-transgenic genome editing across crop species and genotypes. Liu Q, Zhao C, Sun K, Deng Y, Li Z. Mol Plant. 2023 Mar 6;16(3):616-631. doi: 10.1016/j.molp.2023.02.003. Epub 2023 Feb 7. 10.1016/j.molp.2023.02.003 PubMed 36751129 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pS-sg:GFP 10189 bp ds-DNA circular SYN 10-APR-2023
DEFINITION 35S promoter-driven expression of the positive-sense TSWV S RNA
segment encoding sgRNA:GFP fusion in place of viral NSs.
ACCESSION .
VERSION .
KEYWORDS pS-sg:GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10189)
AUTHORS Liu Q, Zhao C, Sun K, Deng Y, Li Z
TITLE Engineered biocontainable RNA virus vectors for non-transgenic
genome editing across crop species and genotypes
JOURNAL Molecular Plant
REFERENCE 2 (bases 1 to 10189)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 10, 2023 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..10189
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 423..768
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
primer_bind 711..731
/label=35S promoter
/note="CaMV 35S promoter, forward primer"
CDS complement(2241..2960)
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVPLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(2286..2307)
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind 2635..2654
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 2894..2915
/label=EGFP-N
/note="EGFP, reverse primer"
misc_RNA complement(2967..3042)
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
terminator 3258..3510
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
misc_feature 3558..3582
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
rep_origin 3710..5052
/label=2u ori
/note="yeast 2u plasmid origin of replication"
protein_bind complement(4673..4720)
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
primer_bind 5093..5111
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(5149..5171)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 5271..5290
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
promoter 5311..5592
/gene="S. cerevisiae TRP1"
/label=TRP1 promoter
CDS 5593..6267
/codon_start=1
/gene="S. cerevisiae TRP1"
/product="phosphoribosylanthranilate isomerase, required
for tryptophan biosynthesis"
/label=TRP1
/note="yeast auxotrophic marker"
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
KK"
rep_origin complement(6370..6825)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6507..6528)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 6719..6738
/label=F1ori-R
/note="F1 origin, reverse primer"
CDS complement(6941..8089)
/codon_start=1
/product="trans-acting replication protein that binds to
and activates oriV"
/label=trfA
/translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
CDS complement(8388..9182)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
rep_origin 9391..10022
/label=oriV
/note="incP origin of replication"
misc_feature 10102..10126
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
ORIGIN
1 aagcttgcat gcctgcaggt caacatggtg gagcacgaca cacttgtcta ctccaaaaat
61 atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata
121 tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg
181 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa
241 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
301 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgataa catggtggag
361 cacgacacac ttgtctactc caaaaatatc aaagatacag tctcagaaga ccaaagggca
421 attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca ttgcccagct
481 atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa atgccatcat
541 tgcgataaag gaaaggccat cgttgaagat gcctctgccg acagtggtcc caaagatgga
601 cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa
661 gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg
721 caagaccctt cctctatata aggaagttca tttcatttgg agaggagagc aattgtgtca
781 attttattca aaccttaaca ctcagtctta caaatcatca cattaaaacc ctaagaaacg
841 actgcggaat acagagttgt actttcgcac cttgaattac atacggtcaa agcatataac
901 aacttctacg atcatcatgt ctaaggttaa gctcactaag gaaaacattg ttgctttgtt
961 gacacaaggc aaagatcttg aatttgaaga agatcagaat ctggtagcat ttaacttcaa
1021 gactttttgt ctggaaaacc ttgaccagat caagaagatg agcattattt catgtctgac
1081 attcctgaag aatcgtcaga gtataatgaa ggttattaag caaagtgatt ttacttttgg
1141 caaaatcact ataaagaaaa cttcagacag gattggagcc actgacatga ccttcagaag
1201 gcttgatagc ttgatcaggg tcaggcttgt cgaggaaact gggaattctg agaatctcaa
1261 tactatcaaa tctaagattg cttctcaccc tctgattcaa gcctatggat tacctcttga
1321 tgatgcaaag tctgtgaggc ttgccataat gctgggaggt agcttacctc ttattgcttc
1381 agttgatagc tttgagatga tcagtgttgt cttggctata tatcaggatg caaaatacaa
1441 agacctcggg atcgatccaa agaagtatga caccagggaa gccttaggaa aagtttgcac
1501 tgtgctaaaa agcaaagcat ttgaaatgaa tgaagatcag gtgaagaaag ggaaagagta
1561 tgctgctata cttagctcca gcaatcctaa tgctaaagga agtattgcta tggaacatta
1621 cagtgaaact cttaacaagt tctatgaaat gttcggggtt aaaaaacaga caaaacttgc
1681 agaacttgct taaaagcagt tgtaagttaa attataaaaa agcctataaa tatataaagc
1741 tttctttatc tttattgctt gtgcttgctt agtgtgttaa atttaaaata agtgtgttta
1801 attaaagttt gctttctgtg tgttgtgctt aataataaac aaaataacaa aaacaacaaa
1861 aaacataaaa taaaaataag aaaaaataaa ataaaataaa ataaaataaa taaataaaaa
1921 taaaaataaa ataaaaacaa aaaacaaaaa acaaaaaaca aaacaaaacc caaatttggc
1981 caaaattgtc cctttcggga tctttttggg tttttgtttt ttaatttttt gttgttttta
2041 tttcattttt tgatttttgt ttgattttgt ttcatttttt gatttttgtt tcattttgat
2101 tttattttta tttttatttt tatttttatg gtttctatta aacaggagaa attcaaaaga
2161 aatgacaaaa cagagaaata attatgagta aagaaagaaa ataaacataa cataattagg
2221 aaaagctgga cacggcaaga ttacttgtac agctcgtcca tgccgagagt gatcccggcg
2281 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg
2341 gcggactggg tgctcaggta gtggttgtcg ggcagcggca cggggccgtc gccgatgggg
2401 gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc
2461 ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg
2521 ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc
2581 ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg
2641 tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg
2701 gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg
2761 taggtcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg
2821 aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac
2881 ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc
2941 tcctcgccct tgctcaccat actagtgcac cgactcggtg ccactttttc aagttgataa
3001 cggactagcc ttattttaac ttgctatttc tagctctaaa accgagaccg aggtccgagg
3061 tctccactag ttatggttat tggtactgtg ttcttattac agtattgtga ttttctaagt
3121 gaggtttgat taagaataaa attatgacac aattgctctg ggtcggcatg gcatctccac
3181 ctcctcgcgg tccgacctgg gcatccgaag gaggacgtcg tccactcgga tggctaaggg
3241 agagctcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta agattgaatc
3301 ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa
3361 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc
3421 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat
3481 cgcgcgcggt gtcatctatg ttactagatc ggaattcaga ttgtcgtttc ccgccttcag
3541 tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt
3601 tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta
3661 tgtgcatgcc aaccacagga gatctcagta aagcgaaaag tgccacctga acgaagcatc
3721 tgtgcttcat tttgtagaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa
3781 tctgagctgc atttttacag aacagaaatg caacgcgaaa gcgctatttt accaacgaag
3841 aatctgtgct tcatttttgt aaaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca
3901 aagaatctga gctgcatttt tacagaacag aaatgcaacg cgagagcgct attttaccaa
3961 caaagaatct atacttcttt tttgttctac aaaaatgcat cccgagagcg ctatttttct
4021 aacaaagcat cttagattac tttttttctc ctttgtgcgc tctataatgc agtctcttga
4081 taactttttg cactgtaggt ccgttaaggt tagaagaagg ctactttggt gtctattttc
4141 tcttccataa aaaaagcctg actccacttc ccgcgtttac tgattactag cgaagctgcg
4201 ggtgcatttt ttcaagataa aggcatcccc gattatattc tataccgatg tggattgcgc
4261 atactttgtg aacagaaagt gatagcgttg atgattcttc attggtcaga aaattatgaa
4321 cggtttcttc tattttgtct ctatatacta cgtataggaa atgtttacat tttcgtattg
4381 ttttcgattc actctatgaa tagttcttac tacaattttt ttgtctaaag agtaatacta
4441 gagataaaca taaaaaatgt agaggtcgag tttagatgca agttcaagga gcgaaaggtg
4501 gatgggtagg ttatataggg atatagcaca gagatatata gcaaagagat acttttgagc
4561 aatgtttgtg gaagcggtat tcgcaatatt ttagtagctc gttacagtcc ggtgcgtttt
4621 tggttttttg aaagtgcgtc ttcagagcgc ttttggtttt caaaagcgct ctgaagttcc
4681 tatactttct agagaatagg aacttcggaa taggaacttc aaagcgtttc cgaaaacgag
4741 cgcttccgaa aatgcaacgc gagctgcgca catacagctc actgttcacg tcgcacctat
4801 atctgcgtgt tgcctgtata tatatataca tgagaagaac ggcatagtgc gtgtttatgc
4861 ttaaatgcgt acttatatgc gtctatttat gtaggatgaa aggtagtcta gtacctcctg
4921 tgatattatc ccattccatg cggggtatcg tatgcttcct tcagcactac cctttagctg
4981 ttctatatgc tgccactcct caattggatt agtctcatcc ttcaatgcta tcatttcctt
5041 tgatattgga tcatactaag aaaccattat tatcatgaca ttaacctata aaaataggcg
5101 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
5161 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
5221 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
5281 gcagattgta ctgagagtgc accatagatc aacgacatta ctatatatat aatataggaa
5341 gcatttaata gaacagcatc gtaatatatg tgtactttgc agttatgacg ccagatggca
5401 gtagtggaag atattcttta ttgaaaaata gcttgtcacc ttacgtacaa tcttgatccg
5461 gagcttttct ttttttgccg attaagaatt aattcggtcg aaaaaagaaa aggagagggc
5521 caagagggag ggcattggtg actattgagc acgtgagtat acgtgattaa gcacacaaag
5581 gcagcttgga gtatgtctgt tattaatttc acaggtagtt ctggtccatt ggtgaaagtt
5641 tgcggcttgc agagcacaga ggccgcagaa tgtgctctag attccgatgc tgacttgctg
5701 ggtattatat gtgtgcccaa tagaaagaga acaattgacc cggttattgc aaggaaaatt
5761 tcaagtcttg taaaagcata taaaaatagt tcaggcactc cgaaatactt ggttggcgtg
5821 tttcgtaatc aacctaagga ggatgttttg gctctggtca atgattacgg cattgatatc
5881 gtccaactgc atggagatga gtcgtggcaa gaataccaag agttcctcgg tttgccagtt
5941 attaaaagac tcgtatttcc aaaagactgc aacatactac tcagtgcagc ttcacagaaa
6001 cctcattcgt ttattccctt gtttgattca gaagcaggtg ggacaggtga acttttggat
6061 tggaactcga tttctgactg ggttggaagg caagagagcc ccgaaagctt acattttatg
6121 ttagctggtg gactgacgcc agaaaatgtt ggtgatgcgc ttagattaaa tggcgttatt
6181 ggtgttgatg taagcggagg tgtggagaca aatggtgtaa aagactctaa caaaatagca
6241 aatttcgtca aaaatgctaa gaaataggtt attactgagt agtatttatt taagtattgt
6301 ttgtgcactt gccgatctat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
6361 cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
6421 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
6481 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
6541 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
6601 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
6661 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
6721 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
6781 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt cgccattcag
6841 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagatcct
6901 tgctggctga acccccagcc ggaactgacc ccacaaggcc ctagcgtttg caatgcacca
6961 ggtcatcatt gacccaggcg tgttccacca ggccgctgcc tcgcaactct tcgcaggctt
7021 cgccgacctg ctcgcgccac ttcttcacgc gggtggaatc cgatccgcac atgaggcgga
7081 aggtttccag cttgagcggg tacggctccc ggtgcgagct gaaatagtcg aacatccgtc
7141 gggccgtcgg cgacagcttg cggtacttct cccatatgaa tttcgtgtag tggtcgccag
7201 caaacagcac gacgatttcc tcgtcgatca ggacctggca acgggacgtt ttcttgccac
7261 ggtccaggac gcggaagcgg tgcagcagcg acaccgattc caggtgccca acgcggtcgg
7321 acgtgaagcc catcgccgtc gcctgtaggc gcgacaggca ttcctcggcc ttcgtgtaat
7381 accggccatt gatcgaccag cccaggtcct ggcaaagctc gtagaacgtg aaggtgatcg
7441 gctcgccgat aggggtgcgc ttcgcgtact ccaacacctg ctgccacacc agttcgtcat
7501 cgtcggcccg cagctcgacg ccggtgtagg tgatcttcac gtccttgttg acgtggaaaa
7561 tgaccttgtt ttgcagcgcc tcgcgcggga ttttcttgtt gcgcgtggtg aacagggcag
7621 agcgggccgt gtcgtttggc atcgctcgca tcgtgtccgg ccacggcgca atatcgaaca
7681 aggaaagctg catttccttg atctgctgct tcgtgtgttt cagcaacgcg gcctgcttgg
7741 cctcgctgac ctgttttgcc aggtcctcgc cggcggtttt tcgcttcttg gtcgtcatag
7801 ttcctcgcgt gtcgatggtc atcgacttcg ccaaacctgc cgcctcctgt tcgagacgac
7861 gcgaacgctc cacggcggcc gatggcgcgg gcagggcagg gggagccagt tgcacgctgt
7921 cgcgctcgat cttggccgta gcttgctggg ccatcgagcc gacggactgg aaggtttcgc
7981 ggggcgcacg catgacggtg cggcttgcga tggtttcggc atcctcggcg gaaaaccccg
8041 cgtcgatcag ttcttgcctg tatgccttcc ggtcaaacgt ccgattcatt caccctcctt
8101 gcgggattgc cccgactcac gccggggcaa tgtgccctta ttcctgattt gacccgcctg
8161 gtgccttggt gtccagataa tccaccttat cggcaatgaa gtcggtcccg tagaccgtct
8221 ggccgtcctt ctcgtacttg gtattccgaa tcttgccctg cacgaatacc agcgacccct
8281 tgcccaaata cttgccgtgg gcctcggcct gagagccaaa acacttgatg cggaagaagt
8341 cggtgcgctc ctgcttgtcg ccggcatcgt tgcgccacat ctaggtacta aaacaattca
8401 tccagtaaaa tataatattt tattttctcc caatcaggct tgatccccag taagtcaaaa
8461 aatagctcga catactgttc ttccccgata tcctccctga tcgaccggac gcagaaggca
8521 atgtcatacc acttgtccgc cctgccgctt ctcccaagat caataaagcc acttactttg
8581 ccatctttca caaagatgtt gctgtctccc aggtcgccgt gggaaaagac aagttcctct
8641 tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa tggggtgtct
8701 tcttcccagt tttcgcaatc cacatcggcc agatcgttat tcagtaagta atccaattcg
8761 gctaagcggc tgtctaagct attcgtatag ggacaatccg atatgtcgat ggagtgaaag
8821 agcctgatgc actccgcata cagctcgata atcttttcag ggctttgttc atcttcatac
8881 tcttccgagc aaaggacgcc atcggcctca ctcatgagca gattgctcca gccatcatgc
8941 cgttcaaggt gcaggacctt tggaacaggc agctttcctt ccagccatag catcatgtcc
9001 ttttcccgtt ccacatcata ggtggtccct ttataccggc tgtccgtcat ttttaaatat
9061 aggttttcat tttctcccac cagcttatat accttagcag gagacattcc ttccgtatct
9121 tttacgcagc ggtatttttc gatcagtttt ttcaattccg gtgatattct cattttagcc
9181 atttattatt tccttcctct tttctacagt atttaaagat accccaagaa gctaattata
9241 acaagacgaa ctccaattca ctgttccttg cattctaaaa ctttaaatac cagaaaacag
9301 ctttttcaaa gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga
9361 aaccacaatt atgggtgatg ctgcaactcg agcgggccgg gagggttcga gaaggggggg
9421 cacccccctt cggcgtgcgc ggtcacgcgc acagggcgca gccctggtta aaaacaaggt
9481 ttataaatat tggtttaaaa gcaggttaaa agacaggtta gcggtggccg aaaaacgggc
9541 ggaaaccctt gcaaatgctg gattttctgc ctgtggacag cccctcaaat gtcaataggt
9601 gcgcccctca tctgtcagca ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt
9661 cagtagtcgc gcccctcaag tgtcaatacc gcagggcact tatccccagg cttgtccaca
9721 tcatctgtgg gaaactcgcg taaaatcagg cgttttcgcc gatttgcgag gctggccagc
9781 tccacgtcgc cggccgaaat cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt
9841 cggcccctca agtgtcaacg tccgcccctc atctgtcagt gagggccaag ttttccgcga
9901 ggtatccaca acgccggcgg ccgcggtgtc tcgcacacgg cttcgacggc gtttctggcg
9961 cgtttgcagg gccatagacg gccgccagcc cagcggcgag ggcaaccagc ccggtgagcg
10021 tctctagtgg actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc
10081 ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag
10141 acaacttaat aacacattgc ggacgttttt aatgtactgg ggtggtttt
//
