| 质粒名称: | FH-TET1-pEF |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 49792 |
| 存储实验室: | Anjana Rao |
|
载体骨架基本信息 |
|
| 载体名称: | pEF1a |
| 载体出品公司: | Invitrogen |
| 空载体大小: | 6200 bp |
| 完整质粒大小: | 12692 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Top10 |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 插入基因名称: | Tet methylcytosine dioxygenase 1,TET1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 6492 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | EF-1alpha |
| 标签/融合蛋白: |
Flag（插入件上的N端子）
HA（插入件上的N端子
|
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | KpnI(未破坏) |
| 3’克隆位点: | XbaI (未破坏) |
| 5’测序引物 | T7 promoter |
| 3’测序引物 | BGH Reverse |
|
文献引用方法 |
|
| 材料和方法部分: | FH-TET1-pEF was a gift from Anjana Rao (Addgene plasmid # 49792 ; http://n2t.net/addgene:49792 ; RRID:Addgene_49792) |
| 参考文献部分: | Conversion of 5-methylcytosine to 5-hydroxymethylcytosine in mammalian DNA by MLL partner TET1. Tahiliani M, Koh KP, Shen Y, Pastor WA, Bandukwala H, Brudno Y, Agarwal S, Iyer LM, Liu DR, Aravind L, Rao A. Science. 2009 May 15;324(5929):930-5. Epub 2009 Apr 16. 10.1126/science.1170116 PubMed 19372391 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS FH-TET1-pEF 12598 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION This plasmid expresses the full-length human TET1 gene with a Flag
and HA tag..
ACCESSION .
VERSION .
KEYWORDS FH-TET1-pEF
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12598)
AUTHORS Tahiliani M, Koh KP, Shen Y, Pastor WA, Bandukwala H, Brudno Y,
Agarwal S, Iyer LM, Liu DR, Aravind L, Rao A
TITLE Conversion of 5-methylcytosine to 5-hydroxymethylcytosine in
mammalian DNA by MLL partner TET1.
JOURNAL Science. 2009 May 15;324(5929):930-5. Epub 2009 Apr 16.
PUBMED 19372391
REFERENCE 2 (bases 1 to 12598)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12598
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 163..1341
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 394..1332
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 1289..1309
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
primer_bind 1358..1377
/label=T7
/note="T7 promoter, forward primer"
promoter 1358..1376
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 1409..1418
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1418..1441
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 1454..1480
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 7919..7960
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
CDS 7970..7987
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(8010..8027)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 8016..8240
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 8286..8714
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(8373..8392)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 8583..8604
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(8723..8743)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 8728..9057
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 8908..9043
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 8970..8989
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 9124..9918
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(9178..9197)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 9788..9807
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 10094..10215
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(10131..10150)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 10185..10204
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(10264..10280)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(10264..10280)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(10277..10299)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 10288..10304
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(10312..10342)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 10357..10378
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(10495..10512)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(10655..11243)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(10735..10754)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(11414..12274)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 12037..12056
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(12275..12379)
/gene="bla"
/label=AmpR promoter
primer_bind complement(12454..12473)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
61 actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga gaggaatctt
121 tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag
181 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
241 accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
301 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
361 ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg
421 cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag
481 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc
541 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc
601 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
661 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
721 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
781 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
841 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
901 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
961 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag
1021 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca
1081 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg
1141 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
1201 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
1261 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
1321 cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac tatagggaga
1381 cccaagctgg ctaggtaagc ttggtaccgc caccatggac tacaaggacg acgatgacaa
1441 gctcgatgga ggatacccct acgacgtgcc cgactacgcc ggaggactcg actctcgatc
1501 ccgccatgca aggccttcca gattagtcag gaaggaagat gtaaacaaaa aaaagaaaaa
1561 cagccaacta cgaaagacaa ccaagggagc caacaaaaat gtggcatcag tcaagacttt
1621 aagccctgga aaattaaagc aattaattca agaaagagat gttaagaaaa aaacagaacc
1681 taaaccaccc gtgccagtca gaagccttct gacaagagct ggagcagcac gcatgaattt
1741 ggataggact gaggttcttt ttcagaaccc agagtcctta acctgcaatg ggtttacaat
1801 ggcgctacga agcacctctc ttagcaggcg actctcccaa cccccactgg tcgtagccaa
1861 atccaaaaag gttccacttt ctaagggttt agaaaagcaa catgattgtg attataagat
1921 actccctgct ttgggagtaa agcactcaga aaatgattcg gttccaatgc aagacaccca
1981 agtccttcct gatatagaga ctctaattgg tgtacaaaat ccctctttac ttaaaggtaa
2041 gagccaagag acaactcagt tttggtccca aagagttgag gattccaaga tcaatatccc
2101 tacccacagt ggccctgcag ctgagatcct tcctgggcca ctggaaggga cacgctgtgg
2161 tgaaggacta ttctctgaag agacattgaa tgataccagt ggttccccaa aaatgtttgc
2221 tcaggacaca gtgtgtgctc cttttcccca aagagcaacc cccaaagtta cctctcaagg
2281 aaaccccagc attcagttag aagagttggg ttcacgagta gaatctctta agttatctga
2341 ttcttacctg gatcccatta aaagtgaaca tgattgctac cccacctcca gtcttaataa
2401 ggttatacct gacttgaacc ttagaaactg cttggctctt ggtgggtcta cgtctcctac
2461 ctctgtaata aaattcctct tggcaggctc aaaacaagcg acccttggtg ctaaaccaga
2521 tcatcaagag gccttcgaag ctactgcaaa tcaacaggaa gtttctgata ccacctcttt
2581 cctaggacag gcctttggtg ctatcccaca tcaatgggaa cttcctggtg ctgacccagt
2641 tcatggtgag gccctgggtg agaccccaga tctaccagag attcctggtg ctattccagt
2701 ccaaggagag gtctttggta ctattttaga ccaacaagaa actcttggta tgagtgggag
2761 tgttgtccca gacttgcctg tcttccttcc tgttcctcca aatccaattg ctacctttaa
2821 tgctccttcc aaatggcctg agccccaaag cactgtctca tatggacttg cagtccaggg
2881 tgctatacag attttgcctt tgggctcagg acacactcct caatcatcat caaactcaga
2941 gaaaaattca ttacctccag taatggctat aagcaatgta gaaaatgaga agcaggttca
3001 tataagcttc ctgccagcta acactcaggg gttcccatta gcccctgaga gaggactctt
3061 ccatgcttca ctgggtatag cccaactctc tcaggctggt cctagcaaat cagacagagg
3121 gagctcccag gtcagtgtaa ccagcacagt tcatgttgtc aacaccacag tggtgactat
3181 gccagtgcca atggtcagta cctcctcttc ttcctatacc actttgctac cgactttgga
3241 aaagaagaaa agaaagcgat gtggggtctg tgaaccctgc cagcagaaga ccaactgtgg
3301 tgaatgcact tactgcaaga acagaaagaa cagccatcag atctgtaaga aaagaaaatg
3361 tgaggagctg aaaaagaaac catctgttgt tgtgcctctg gaggttataa aggaaaacaa
3421 gaggccccag agggaaaaga agcccaaagt tttaaaggca gattttgaca acaaaccagt
3481 aaatggcccc aagtcagaat ccatggacta cagtagatgt ggtcatgggg aagaacaaaa
3541 attggaattg aacccacata ctgttgaaaa tgtaactaaa aatgaagaca gcatgacagg
3601 catcgaggtg gagaagtgga cacaaaacaa gaaatcacag ttaactgatc acgtgaaagg
3661 agattttagt gctaatgtcc cagaagctga aaaatcgaaa aactctgaag ttgacaagaa
3721 acgaaccaaa tctccaaaat tgtttgtaca aaccgtaaga aatggcatta aacatgtaca
3781 ctgtttacca gctgaaacaa atgtttcatt taaaaaattc aatattgaag aattcggcaa
3841 gacattggaa aacaattctt ataaattcct aaaagacact gcaaaccata aaaacgctat
3901 gagctctgtt gctactgata tgagttgtga tcatctcaag gggagaagta acgttttagt
3961 attccagcag cctggcttta actgcagttc cattccacat tcttcacact ccatcataaa
4021 tcatcatgct agtatacaca atgaaggtga tcaaccaaaa actcctgaga atataccaag
4081 taaagaacca aaagatggat ctcccgttca accaagtctc ttatcgttaa tgaaagatag
4141 gagattaaca ttggagcaag tggtagccat agaggccctg actcaactct cagaagcccc
4201 atcagagaat tcctccccat caaagtcaga gaaggatgag gaatcagagc agagaacagc
4261 cagtttgctt aatagctgca aagctatcct ctacactgta agaaaagacc tccaagaccc
4321 aaacttacag ggagagccac caaaacttaa tcactgtcca tctttggaaa aacaaagttc
4381 atgcaacacg gtggttttca atgggcaaac tactaccctt tccaactcac atatcaactc
4441 agctactaac caagcatcca caaagtcaca tgaatattca aaagtcacaa attcattatc
4501 tctttttata ccaaaatcaa attcatccaa gattgacacc aataaaagta ttgctcaagg
4561 gataattact cttgacaatt gttccaatga tttgcatcag ttgccaccaa gaaataatga
4621 agtggagtat tgcaaccagt tactggacag cagcaaaaaa ttggactcag atgatctatc
4681 atgtcaggat gcaacccata cccaaattga ggaagatgtt gcaacacagt tgacacaact
4741 tgcttcgata attaagatca attatataaa accagaggac aaaaaagttg aaagtacacc
4801 aacaagcctt gtcacatgta atgtacagca aaaatacaat caggagaagg gcacaatgca
4861 acagaaacca ccttcaagtg tacacaataa tcatggttca tcattaacaa aacaaaagaa
4921 cccaacccag aaaaagacaa aatccacccc atcaagagat cggcggaaaa agaagcccac
4981 agttgtaagt tatcaagaaa atgatcggca gaagtgggaa aagttgtcct atatgtatgg
5041 cacaatatgc gacatttgga tagcatcgaa atttcaaaat tttgggcaat tttgtccaca
5101 tgattttcct actgtatttg ggaaaatttc ttcctcgacc aaaatatgga aaccactggc
5161 tcaaacgagg tccattatgc aacccaaaac agtatttcca ccactcactc agataaaatt
5221 acagagatat cctgaatcag cagaggaaaa ggtgaaggtt gaaccattgg attcactcag
5281 cttatttcat cttaaaacgg aatccaacgg gaaggcattc actgataaag cttataattc
5341 tcaggtacag ttaacggtga atgccaatca gaaagcccat cctttgaccc agccctcctc
5401 tccacctaac cagtgtgcta acgtgatggc aggcgatgac caaatacggt ttcagcaggt
5461 tgttaaggag caactcatgc atcagagact gccaacattg cctggtatct ctcatgaaac
5521 acccttaccg gagtcagcac taactctcag gaatgtaaat gtagtgtgtt caggtggaat
5581 tacagtggtt tctaccaaaa gtgaagagga agtctgttca tccagttttg gaacatcaga
5641 attttccaca gtggacagtg cacagaaaaa ttttaatgat tatgccatga acttctttac
5701 taaccctaca aaaaacctag tgtctataac taaagattct gaactgccca cctgcagctg
5761 tcttgatcga gttatacaaa aagacaaagg cccatattat acacaccttg gggcaggacc
5821 aagtgttgct gctgtcaggg aaatcatgga gaataggtat ggtcaaaaag gaaacgcaat
5881 aaggatagaa atagtagtgt acaccggtaa agaagggaaa agctctcatg ggtgtccaat
5941 tgctaagtgg gttttaagaa gaagcagtga tgaagaaaaa gttctttgtt tggtccggca
6001 gcgtacaggc caccactgtc caactgctgt gatggtggtg ctcatcatgg tgtgggatgg
6061 catccctctt ccaatggccg accggctata cacagagctc acagagaatc taaagtcata
6121 caatgggcac cctaccgaca gaagatgcac cctcaatgaa aatcgtacct gtacatgtca
6181 aggaattgat ccagagactt gtggagcttc attctctttt ggctgttcat ggagtatgta
6241 ctttaatggc tgtaagtttg gtagaagccc aagccccaga agatttagaa ttgatccaag
6301 ctctccctta catgaaaaaa accttgaaga taacttacag agtttggcta cacgattagc
6361 tccaatttat aagcagtatg ctccagtagc ttaccaaaat caggtggaat atgaaaatgt
6421 tgcccgagaa tgtcggcttg gcagcaagga aggtcgtccc ttctctgggg tcactgcttg
6481 cctggacttc tgtgctcatc cccacaggga cattcacaac atgaataatg gaagcactgt
6541 ggtttgtacc ttaactcgag aagataaccg ctctttgggt gttattcctc aagatgagca
6601 gctccatgtg ctacctcttt ataagctttc agacacagat gagtttggct ccaaggaagg
6661 aatggaagcc aagatcaaat ctggggccat cgaggtcctg gcaccccgcc gcaaaaaaag
6721 aacgtgtttc actcagcctg ttccccgttc tggaaagaag agggctgcga tgatgacaga
6781 ggttcttgca cataagataa gggcagtgga aaagaaacct attccccgaa tcaagcggaa
6841 gaataactca acaacaacaa acaacagtaa gccttcgtca ctgccaacct tagggagtaa
6901 cactgagacc gtgcaacctg aagtaaaaag tgaaaccgaa ccccatttta tcttaaaaag
6961 ttcagacaac actaaaactt attcgctgat gccatccgct cctcacccag tgaaagaggc
7021 atctccaggc ttctcctggt ccccgaagac tgcttcagcc acaccagctc cactgaagaa
7081 tgacgcaaca gcctcatgcg ggttttcaga aagaagcagc actccccact gtacgatgcc
7141 ttcgggaaga ctcagtggtg ccaatgcagc tgctgctgat ggccctggca tttcacagct
7201 tggcgaagtg gctcctctcc ccaccctgtc tgctcctgtg atggagcccc tcattaattc
7261 tgagccttcc actggtgtga ctgagccgct aacgcctcat cagccaaacc accagccctc
7321 cttcctcacc tctcctcaag accttgcctc ttctccaatg gaagaagatg agcagcattc
7381 tgaagcagat gagcctccat cagacgaacc cctatctgat gaccccctgt cacctgctga
7441 ggagaaattg ccccacattg atgagtattg gtcagacagt gagcacatct ttttggatgc
7501 aaatattggt ggggtggcca tcgcacctgc tcacggctcg gttttgattg agtgtgcccg
7561 gcgagagctg cacgctacca ctcctgttga gcaccccaac cgtaatcatc caacccgcct
7621 ctcccttgtc ttttaccagc acaaaaacct aaataagccc caacatggtt ttgaactaaa
7681 caagattaag tttgaggcta aagaagctaa gaataagaaa atgaaggcct cagagcaaaa
7741 agaccaggca gctaatgaag gtccagaaca gtcctctgaa gtaaatgaat tgaaccaaat
7801 tccttctcat aaagcattaa cattaaccca tgacaatgtt gtcaccgtgt ccccttatgc
7861 tctcacacac gttgcggggc cctataacca ttgggtctga tctagagggc ccttcgaagg
7921 taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtc atcatcacca
7981 tcaccattga gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
8041 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
8101 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
8161 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
8221 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
8281 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
8341 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
8401 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
8461 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
8521 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
8581 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
8641 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
8701 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
8761 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
8821 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
8881 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
8941 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
9001 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
9061 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt
9121 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
9181 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
9241 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
9301 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
9361 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
9421 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
9481 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
9541 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
9601 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
9661 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
9721 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
9781 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
9841 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
9901 cttgacgagt tcttctgagc gggactctgg ggttcgcgaa atgaccgacc aagcgacgcc
9961 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
10021 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
10081 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
10141 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
10201 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
10261 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
10321 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
10381 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
10441 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
10501 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
10561 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
10621 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
10681 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
10741 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
10801 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
10861 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
10921 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
10981 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
11041 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
11101 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
11161 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
11221 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
11281 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
11341 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
11401 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
11461 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
11521 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
11581 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
11641 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
11701 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
11761 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
11821 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
11881 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
11941 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
12001 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
12061 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
12121 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
12181 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
12241 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
12301 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
12361 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
12421 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
12481 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
12541 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggtt
//
