psPAX2-D64V

LOCUS       psPAX2-D64V               10644 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  psPAX2-D64V is derived from psPAX2 plasmid. It is a packaging 
            plasmid for making Integrase deficient lentiviral vectors since has 
            a point mutation in the integrase gene..
ACCESSION   .
VERSION     .
KEYWORDS    psPAX2-D64V
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10644)
  AUTHORS   Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ, 
            Scharenberg AM
  TITLE     Tracking genome engineering outcome at individual DNA breakpoints.
  JOURNAL   Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648.
  PUBMED    21743461
REFERENCE   2  (bases 1 to 10644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10644
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..196
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      47..182
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     109..128
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    202..336
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(239..258)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     293..312
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(404..421)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(575..1163)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(655..674)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1334..2194)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1957..1976
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2195..2299)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        2330..2709
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          2922..3938
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3862..3884
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3946..3965
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             4044..5546
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             5339..8350
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLVCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    8035..8152
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    8924..9157
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9342..9386
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             9680..9706
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     primer_bind     complement(9991..10010)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    10056..10111
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(10110..10129)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(10472..10488)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10472..10488)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10485..10507)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10496..10512
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10520..10550)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10565..10586
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
       61 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
      121 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
      181 cctaggcttt tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc
      241 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
      301 tccaaactca tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac
      361 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
      421 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
      481 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
      541 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      601 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      661 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      721 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
      781 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      841 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      901 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      961 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     1021 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     1081 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     1141 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     1201 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     1261 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     1321 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     1381 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     1441 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1501 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1561 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1621 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1681 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1741 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1801 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1861 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1921 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1981 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     2041 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     2101 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     2161 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     2221 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     2281 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg acattgatta
     2341 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     2401 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     2461 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     2521 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     2581 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     2641 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     2701 attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc
     2761 ccacccccaa ttttgtattt atttattttt taattatttt gtgcagggcg aggcggagag
     2821 gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc
     2881 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgcgctgcc
     2941 ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg
     3001 cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct
     3061 tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag gggctccggg
     3121 agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga
     3181 gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc
     3241 tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg
     3301 gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag
     3361 ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc cgagttgctg
     3421 agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc
     3481 cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg
     3541 agggctcggg ggaggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga
     3601 gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc
     3661 aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg
     3721 cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc
     3781 gccgccgtcc ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc
     3841 gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct
     3901 ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta
     3961 ttgtgctgtc tcatcatttt ggcaaagaat tcgggccgcg ttgacgcgca cggcaagagg
     4021 cgaggggcgg cgactggtga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     4081 tagatcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     4141 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     4201 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     4261 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     4321 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     4381 aaaaagcaca gcaagcagca gctgacacag gacacagcaa tcaggtcagc caaaattacc
     4441 ctatagtgca gaacatccag gggcaaatgg tacatcaggc catatcacct agaactttaa
     4501 atgcatgggt aaaagtagta gaagagaagg ctttcagccc agaagtgata cccatgtttt
     4561 cagcattatc agaaggagcc accccacaag atttaaacac catgctaaac acagtggggg
     4621 gacatcaagc agccatgcaa atgttaaaag agaccatcaa tgaggaagct gcagaatggg
     4681 atagagtgca tccagtgcat gcagggccta ttgcaccagg ccagatgaga gaaccaaggg
     4741 gaagtgacat agcaggaact actagtaccc ttcaggaaca aataggatgg atgacacata
     4801 atccacctat cccagtagga gaaatctata aaagatggat aatcctggga ttaaataaaa
     4861 tagtaagaat gtatagccct accagcattc tggacataag acaaggacca aaggaaccct
     4921 ttagagacta tgtagaccga ttctataaaa ctctaagagc cgagcaagct tcacaagagg
     4981 taaaaaattg gatgacagaa accttgttgg tccaaaatgc gaacccagat tgtaagacta
     5041 ttttaaaagc attgggacca ggagcgacac tagaagaaat gatgacagca tgtcagggag
     5101 tggggggacc cggccataaa gcaagagttt tggctgaagc aatgagccaa gtaacaaatc
     5161 cagctaccat aatgatacag aaaggcaatt ttaggaacca aagaaagact gttaagtgtt
     5221 tcaattgtgg caaagaaggg cacatagcca aaaattgcag ggcccctagg aaaaagggct
     5281 gttggaaatg tggaaaggaa ggacaccaaa tgaaagattg tactgagaga caggctaatt
     5341 ttttagggaa gatctggcct tcccacaagg gaaggccagg gaattttctt cagagcagac
     5401 cagagccaac agccccacca gaagagagct tcaggtttgg ggaagagaca acaactccct
     5461 ctcagaagca ggagccgata gacaaggaac tgtatccttt agcttccctc agatcactct
     5521 ttggcagcga cccctcgtca caataaagat aggggggcaa ttaaaggaag ctctattaga
     5581 tacaggagca gatgatacag tattagaaga aatgaatttg ccaggaagat ggaaaccaaa
     5641 aatgataggg ggaattggag gttttatcaa agtaagacag tatgatcaga tactcataga
     5701 aatctgcgga cataaagcta taggtacagt attagtagga cctacacctg tcaacataat
     5761 tggaagaaat ctgttgactc agattggctg cactttaaat tttcccatta gtcctattga
     5821 gactgtacca gtaaaattaa agccaggaat ggatggccca aaagttaaac aatggccatt
     5881 gacagaagaa aaaataaaag cattagtaga aatttgtaca gaaatggaaa aggaaggaaa
     5941 aatttcaaaa attgggcctg aaaatccata caatactcca gtatttgcca taaagaaaaa
     6001 agacagtact aaatggagaa aattagtaga tttcagagaa cttaataaga gaactcaaga
     6061 tttctgggaa gttcaattag gaataccaca tcctgcaggg ttaaaacaga aaaaatcagt
     6121 aacagtactg gatgtgggcg atgcatattt ttcagttccc ttagataaag acttcaggaa
     6181 gtatactgca tttaccatac ctagtataaa caatgagaca ccagggatta gatatcagta
     6241 caatgtgctt ccacagggat ggaaaggatc accagcaata ttccagtgta gcatgacaaa
     6301 aatcttagag ccttttagaa aacaaaatcc agacatagtc atctatcaat acatggatga
     6361 tttgtatgta ggatctgact tagaaatagg gcagcataga acaaaaatag aggaactgag
     6421 acaacatctg ttgaggtggg gatttaccac accagacaaa aaacatcaga aagaacctcc
     6481 attcctttgg atgggttatg aactccatcc tgataaatgg acagtacagc ctatagtgct
     6541 gccagaaaag gacagctgga ctgtcaatga catacagaaa ttagtgggaa aattgaattg
     6601 ggcaagtcag atttatgcag ggattaaagt aaggcaatta tgtaaacttc ttaggggaac
     6661 caaagcacta acagaagtag taccactaac agaagaagca gagctagaac tggcagaaaa
     6721 cagggagatt ctaaaagaac cggtacatgg agtgtattat gacccatcaa aagacttaat
     6781 agcagaaata cagaagcagg ggcaaggcca atggacatat caaatttatc aagagccatt
     6841 taaaaatctg aaaacaggaa agtatgcaag aatgaagggt gcccacacta atgatgtgaa
     6901 acaattaaca gaggcagtac aaaaaatagc cacagaaagc atagtaatat ggggaaagac
     6961 tcctaaattt aaattaccca tacaaaagga aacatgggaa gcatggtgga cagagtattg
     7021 gcaagccacc tggattcctg agtgggagtt tgtcaatacc cctcccttag tgaagttatg
     7081 gtaccagtta gagaaagaac ccataatagg agcagaaact ttctatgtag atggggcagc
     7141 caatagggaa actaaattag gaaaagcagg atatgtaact gacagaggaa gacaaaaagt
     7201 tgtcccccta acggacacaa caaatcagaa gactgagtta caagcaattc atctagcttt
     7261 gcaggattcg ggattagaag taaacatagt gacagactca caatatgcat tgggaatcat
     7321 tcaagcacaa ccagataaga gtgaatcaga gttagtcagt caaataatag agcagttaat
     7381 aaaaaaggaa aaagtctacc tggcatgggt accagcacac aaaggaattg gaggaaatga
     7441 acaagtagat aaattggtca gtgctggaat caggaaagta ctatttttag atggaataga
     7501 taaggcccaa gaagaacatg agaaatatca cagtaattgg agagcaatgg ctagtgattt
     7561 taacctacca cctgtagtag caaaagaaat agtagccagc tgtgataaat gtcagctaaa
     7621 aggggaagcc atgcatggac aagtagactg tagcccagga atatggcagc tagtttgtac
     7681 acatttagaa ggaaaagtta tcttggtagc agttcatgta gccagtggat atatagaagc
     7741 agaagtaatt ccagcagaga cagggcaaga aacagcatac ttcctcttaa aattagcagg
     7801 aagatggcca gtaaaaacag tacatacaga caatggcagc aatttcacca gtactacagt
     7861 taaggccgcc tgttggtggg cggggatcaa gcaggaattt ggcattccct acaatcccca
     7921 aagtcaagga gtaatagaat ctatgaataa agaattaaag aaaattatag gacaggtaag
     7981 agatcaggct gaacatctta agacagcagt acaaatggca gtattcatcc acaattttaa
     8041 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     8101 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     8161 ttacagggac agcagagatc cagtttggaa aggaccagca aagctcctct ggaaaggtga
     8221 aggggcagta gtaatacaag ataatagtga cataaaagta gtgccaagaa gaaaagcaaa
     8281 gatcatcagg gattatggaa aacagatggc aggtgatgat tgtgtggcaa gtagacagga
     8341 tgaggattaa cacatggaat tctgcaacaa ctgctgttta tccatttcag aattgggtgt
     8401 cgacatagca gaataggcgt tactcgacag aggagagcaa gaaatggagc cagtagatcc
     8461 tagactagag ccctggaagc atccaggaag tcagcctaaa actgcttgta ccaattgcta
     8521 ttgtaaaaag tgttgctttc attgccaagt ttgtttcatg acaaaagcct taggcatctc
     8581 ctatggcagg aagaagcgga gacagcgacg aagagctcat cagaacagtc agactcatca
     8641 agcttctcta tcaaagcagt aagtagtaca tgtaatgcaa cctataatag tagcaatagt
     8701 agcattagta gtagcaataa taatagcaat agttgtgtgg tccatagtaa tcatagaata
     8761 taggaaaatg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     8821 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     8881 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     8941 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     9001 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     9061 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     9121 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     9181 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     9241 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     9301 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     9361 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     9421 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     9481 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     9541 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     9601 acagagacag atccattcga ttagtgaacg gatccttggc acttatctgg gacgatctgc
     9661 ggagcctgtg cctcttcagc taccaccgct tgagagactt actcttgatt gtaacgagga
     9721 ttgtggaact tctgggacgc agggggtggg aagccctcaa atattggtgg aatctcctac
     9781 aatattggag tcaggagcta aagaatagtg ctgttagctt gctcaatgcc acagccatag
     9841 cagtagctga ggggacagat agggttatag aagtagtaca aggagcttgt agagctattc
     9901 gccacatacc tagaagaata agacagggct tggaaaggat tttgctataa gctcgaaaca
     9961 accggtacct ctagaactat agctagcaga tctttttccc tctgccaaaa attatgggga
    10021 catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc
    10081 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
    10141 tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc
    10201 tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt
    10261 ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt
    10321 tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac tagccagatt
    10381 tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct
    10441 cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
    10501 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
    10561 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
    10621 gaaacctgtc gtgccagcgg atcc
//