pESC-TRP

价格:1500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Agilent 安捷伦
载体名称: pESC-TRP, pESC TRP
质粒类型: 酿酒酵母蛋白表达载体
表达水平: 高拷贝
诱导方法: 半乳糖
启动子: GAL1和GAL10
克隆方法: 多克隆位点,限制性内切酶
载体大小: 6525 bp
5' 测序引物及序列: GAL1-F: ATTTTCGGTTTGTATTACTTC;
GAL10-F: GGTGGTAATGCCATGTAATATG
3' 测序引物及序列: GALI-R: GTTCTTAATACTAACATAACT
GAL10-R: GGCAAGGTAGACAAGCCGACAAC
载体标签: C-Flag, C-Myc
载体抗性: 氨苄
筛选标记: TRP1
备注: 利用半乳糖诱导,可以同时使两个基因在酿酒酵母中表达。
产品目录号: 217453
稳定性: 稳定 Stable
组成型/诱导型: 诱导型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pESC-TRP载体图谱

pESC-TRP多克隆位点

载体简介

载体序列

LOCUS       pESC-TRP                6525 bp    DNA     circular SYN 16-SEP-2005
DEFINITION  Cloning vector pESC-TRP, complete sequence.
ACCESSION   AF063848
VERSION     AF063848.1  GI:3228558
SOURCE      Cloning vector pESC-TRP.
  ORGANISM  Cloning vector pESC-TRP
            artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..6525
                     /organism="Cloning vector pESC-TRP"
                     /mol_type="genomic DNA"
                     /specific_host="Escherichia coli K12"
                     /db_xref="taxon:76665"
                     /vntifkey="98"
     CDS             complement(4126..4983)
                     /vntifkey="4"
                     /label=AmpR
     rep_origin      5117..6272
                     /vntifkey="33"
                     /label=2\micron\ori
     CDS             468..1139
                     /vntifkey="4"
                     /label=TRP1
                     /note="yeast TRP1 ORF"
     terminator      complement(1779..1943)
                     /vntifkey="43"
                     /label=ADH1\terminator
     terminator      2932..3121
                     /vntifkey="43"
                     /label=CYC1\terminator
     rep_origin      complement(1373..1679)
                     /vntifkey="33"
                     /label=f1\ori
     promoter        2374..2828
                     /vntifkey="29"
                     /label=GAL1\promoter
     promoter        complement(2162..2373)
                     /vntifkey="29"
                     /label=GAL10\promoter
     misc_feature    2870..2905
                     /vntifkey="21"
                     /label=c-myc\tag
     misc_feature    complement(2092..2118)
                     /vntifkey="21"
                     /label=FLAG\tag
     misc_feature    complement(2074..2157)
                     /vntifkey="21"
                     /label=MCS1
     misc_feature    2830..2927
                     /vntifkey="21"
                     /label=MCS2
     rep_origin      3308..3975
                     /vntifkey="33"
                     /label=pUC\ori
BASE COUNT     1845 a      1380 c      1462 g      1838 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat 
      241 atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa 
      301 aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa 
      361 gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat 
      421 tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta 
      481 atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg 
      541 cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa 
      601 agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa 
      661 atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg 
      721 ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt 
      781 ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag 
      841 actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg 
      901 attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg 
      961 gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa 
     1021 atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg 
     1081 agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat 
     1141 aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga 
     1201 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt 
     1261 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa 
     1321 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca 
     1381 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc 
     1441 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg 
     1501 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg 
     1561 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg 
     1621 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg 
     1681 ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga 
     1741 tcggtgcggg cctcttcgct attacgccag ctgaattgga gcgacctcat gctatacctg 
     1801 agaaagcaac ctgacctaca ggaaagagtt actcaagaat aagaattttc gttttaaaac 
     1861 ctaagagtca ctttaaaatt tgtatacact tatttttttt ataacttatt taataataaa 
     1921 aatcataaat cataagaaat tcgcttattt agaagtgtca acaacgtatc taccaacgat 
     1981 ttgacccttt tccatctttt cgtaaatttc tggcaaggta gacaagccga caaccttgat 
     2041 tggagacttg accaaacctc tggcgaagaa ttgttaatta agagctcaga tcttatcgtc 
     2101 gtcatccttg taatccatcg atactagtgc ggccgccctt tagtgagggt tgaattcgaa 
     2161 ttttcaaaaa ttcttacttt ttttttggat ggacgcaaag aagtttaata atcatattac 
     2221 atggcattac caccatatac atatccatat acatatccat atctaatctt acttatatgt 
     2281 tgtggaaatg taaagagccc cattatctta gcctaaaaaa accttctctt tggaactttc 
     2341 agtaatacgc ttaactgctc attgctatat tgaagtacgg attagaagcc gccgagcggg 
     2401 tgacagccct ccgaaggaag actctcctcc gtgcgtcctc gtcttcaccg gtcgcgttcc 
     2461 tgaaacgcag atgtgcctcg cgccgcactg ctccgaacaa taaagattct acaatactag 
     2521 cttttatggt tatgaagagg aaaaattggc agtaacctgg ccccacaaac cttcaaatga 
     2581 acgaatcaaa ttaacaacca taggatgata atgcgattag ttttttagcc ttatttctgg 
     2641 ggtaattaat cagcgaagcg atgatttttg atctattaac agatatataa atgcaaaaac 
     2701 tgcataacca ctttaactaa tactttcaac attttcggtt tgtattactt cttattcaaa 
     2761 tgtaataaaa gtatcaacaa aaaattgtta atatacctct atactttaac gtcaaggaga 
     2821 aaaaaccccg gatccgtaat acgactcact atagggcccg ggcgtcgaca tggaacagaa 
     2881 gttgatttcc gaagaagacc tcgagtaagc ttggtaccgc ggctagctaa gatccgctct 
     2941 aaccgaaaag gaaggagtta gacaacctga agtctaggtc cctatttatt tttttatagt 
     3001 tatgttagta ttaagaacgt tatttatatt tcaaattttt cttttttttc tgtacagacg 
     3061 cgtgtacgca tgtaacatta tactgaaaac cttgcttgag aaggttttgg gacgctcgaa 
     3121 gatccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 
     3181 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 
     3241 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 
     3301 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 
     3361 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 
     3421 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 
     3481 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 
     3541 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 
     3601 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 
     3661 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 
     3721 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 
     3781 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 
     3841 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 
     3901 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 
     3961 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 
     4021 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 
     4081 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 
     4141 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 
     4201 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 
     4261 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 
     4321 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 
     4381 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 
     4441 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 
     4501 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 
     4561 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 
     4621 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 
     4681 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 
     4741 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 
     4801 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 
     4861 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 
     4921 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 
     4981 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 
     5041 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 
     5101 aaaagtgcca cctgaacgaa gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga 
     5161 gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg 
     5221 cgaaagcgct attttaccaa cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa 
     5281 cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg 
     5341 caacgcgaga gcgctatttt accaacaaag aatctatact tcttttttgt tctacaaaaa 
     5401 tgcatcccga gagcgctatt tttctaacaa agcatcttag attacttttt ttctcctttg 
     5461 tgcgctctat aatgcagtct cttgataact ttttgcactg taggtccgtt aaggttagaa 
     5521 gaaggctact ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc acttcccgcg 
     5581 tttactgatt actagcgaag ctgcgggtgc attttttcaa gataaaggca tccccgatta 
     5641 tattctatac cgatgtggat tgcgcatact ttgtgaacag aaagtgatag cgttgatgat 
     5701 tcttcattgg tcagaaaatt atgaacggtt tcttctattt tgtctctata tactacgtat 
     5761 aggaaatgtt tacattttcg tattgttttc gattcactct atgaatagtt cttactacaa 
     5821 tttttttgtc taaagagtaa tactagagat aaacataaaa aatgtagagg tcgagtttag 
     5881 atgcaagttc aaggagcgaa aggtggatgg gtaggttata tagggatata gcacagagat 
     5941 atatagcaaa gagatacttt tgagcaatgt ttgtggaagc ggtattcgca atattttagt 
     6001 agctcgttac agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag agcgcttttg 
     6061 gttttcaaaa gcgctctgaa gttcctatac tttctagaga ataggaactt cggaatagga 
     6121 acttcaaagc gtttccgaaa acgagcgctt ccgaaaatgc aacgcgagct gcgcacatac 
     6181 agctcactgt tcacgtcgca cctatatctg cgtgttgcct gtatatatat atacatgaga 
     6241 agaacggcat agtgcgtgtt tatgcttaaa tgcgtactta tatgcgtcta tttatgtagg 
     6301 atgaaaggta gtctagtacc tcctgtgata ttatcccatt ccatgcgggg tatcgtatgc 
     6361 ttccttcagc actacccttt agctgttcta tatgctgcca ctcctcaatt ggattagtct 
     6421 catccttcaa tgctatcatt tcctttgata ttggatcata ttaagaaacc attattatca 
     6481 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc 
//

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