pCS2+8CmCherry

价格:1100元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: 爱迪基因
载体名称: pCS2+8CmCherry
质粒类型: 克隆载体;哺乳动物表达载体;广宿主载体;体外表达载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: simian CMV IE94、lac、T3、SP6
载体大小: 4848 bp
5' 测序引物及序列: SP6 fwd(MCS1)、T3 fwd(MCS2)
3' 测序引物及序列: EBV rev(MCS1)、SV40 polyA rev(MCS2)
载体标签: mCherry
载体抗性: 氨苄青霉素
克隆菌株: DH10B
表达细胞: 原核细胞、哺乳动物、非洲爪蟾、鸟类、斑马鱼。
备注: pCS2+8CmCherry载体能够在多种细胞中进行高水平的瞬表达。 pCS2+8CmCherry载体同时也能进行体外转录和翻译。
产品目录号: #34935
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pCS2+8CmCherry载体图谱



pCS2+8CmCherry 多克

隆位点1

pCS2+8CmCherry 多克

隆位点2

载体简介

载体序列

LOCUS       pCS2+8CmCherry Sequencing Result	4848 bp 	DNA	SYN
DEFINITION  pCS2+8CmCherry Sequencing Result
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTNAME|pCS2+8CmCherry|
FEATURES             Location/Qualifiers
     source          1..4848
                     /organism="pCS2+8CmCherry Sequencing Result"
                     /mol_type="other DNA"
     misc_feature    186..208
                     /label="M13_pUC_rev_primer"
     promoter        207..225
                     /label="M13_reverse_primer"
     promoter        242..261
                     /label="T3_promoter"
     misc_feature    464..483
                     /label="EBV_rev_primer"
     CDS             complement(558..1268)
                     /label="ORF frame 2"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
                     NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
                     ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
                     YERAEGRHSTGGMDELYK*"
     gene            complement(561..1265)
                     /label="mCherry"
                     /gene="mCherry"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
                     NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
                     ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
                     YERAEGRHSTGGMDELYK*"
     misc_feature    complement(1035..1088)
                     /label="Q66M (mCherry)"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
                     NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
                     ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
                     YERAEGRHSTGGMDELYK*"
     misc_feature    complement(1355..1372)
                     /label="Sp6_primer"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
                     NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
                     ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
                     YERAEGRHSTGGMDELYK*"
     misc_feature    complement(1448..1489)
                     /label="CMV_fwd_primer"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
                     NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
                     ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
                     YERAEGRHSTGGMDELYK*"
     CDS             complement(1958..2482)
                     /label="ORF frame 3"
                     /translation="MRRYRARPIRHSGCATVGKGDRCGPLRYYASRPPIQYGVYGLMP
                     IQYGGSGPVPIQYGVYGLVPIQYGGSGPQPIQYGGLGTMPIQYGGPGTVPTGEGSTWH
                     GAKFEEGSWPCAKSAILNWHGANNGGHIGYMPGSIYRQYPIWPYANMAIGQVQYYVLA
                     LCHIVFHIWVFLLT*"
     rep_origin      2479..2785
                     /label="f1_origin"
                     /translation="MRRYRARPIRHSGCATVGKGDRCGPLRYYASRPPIQYGVYGLMP
                     IQYGGSGPVPIQYGVYGLVPIQYGGSGPQPIQYGGLGTMPIQYGGPGTVPTGEGSTWH
                     GAKFEEGSWPCAKSAILNWHGANNGGHIGYMPGSIYRQYPIWPYANMAIGQVQYYVLA
                     LCHIVFHIWVFLLT*"
     promoter        2978..3006
                     /label="AmpR_promoter"
                     /translation="MRRYRARPIRHSGCATVGKGDRCGPLRYYASRPPIQYGVYGLMP
                     IQYGGSGPVPIQYGVYGLVPIQYGGSGPQPIQYGGLGTMPIQYGGPGTVPTGEGSTWH
                     GAKFEEGSWPCAKSAILNWHGANNGGHIGYMPGSIYRQYPIWPYANMAIGQVQYYVLA
                     LCHIVFHIWVFLLT*"
     gene            3048..3908
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MRRYRARPIRHSGCATVGKGDRCGPLRYYASRPPIQYGVYGLMP
                     IQYGGSGPVPIQYGVYGLVPIQYGGSGPQPIQYGGLGTMPIQYGGPGTVPTGEGSTWH
                     GAKFEEGSWPCAKSAILNWHGANNGGHIGYMPGSIYRQYPIWPYANMAIGQVQYYVLA
                     LCHIVFHIWVFLLT*"
     CDS             3048..3908
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      4063..4682
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgCAGCTGGC
   61 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC
  121 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTCCC GGCTCGTATG TTGTGTGGAA
  181 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCGCG
  241 CAATTAACCC TCACTAAAGG GAACAAAAGC TGGGTACCGG GCCCAATGCA TTGGCGCCGC
  301 GGCCGCGAAT TAAAAAACCT CCCACACCTC CCCCTGAACC TGAAACATAA AATGAATGCA
  361 ATTGTTGTTG TTAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC
  421 ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC
  481 ATCAATGTAT CTTATCATGT CTGGATCTAC GTAATACGAC TCACTATAGT TCTAGAGGCT
  541 CGAGAGGCCT TGAATTCTTA CTTGTACAGC TCGTCCATGC CGCCGGTGGA GTGGCGGCCC
  601 TCGGCGCGTT CGTACTGTTC CACGATGGTG TAGTCCTCGT TGTGGGAGGT GATGTCCAAC
  661 TTGATGTTGA CGTTGTAGGC GCCGGGCAGC TGCACGGGCT TCTTGGCCTT GTAGGTGGTC
  721 TTGACCTCAG CGTCGTAGTG GCCGCCGTCC TTCAGCTTCA GCCTCTGCTT GATCTCGCCC
  781 TTCAGGGCGC CGTCCTCGGG GTACATCCGC TCGGAGGAGG CCTCCCAGCC CATGGTCTTC
  841 TTCTGCATTA CGGGGCCGTC GGAGGGGAAG TTGGTGCCGC GCAGCTTCAC CTTGTAGATG
  901 AACTCGCCGT CCTGCAGGGA GGAGTCCTGG GTCACGGTCA CCACGCCGCC GTCCTCGAAG
  961 TTCATCACGC GCTCCCACTT GAAGCCCTCG GGGAAGGACA GCTTCAAGTA GTCGGGGATG
 1021 TCGGCGGGGT GCTTCACGTA GGCCTTGGAG CCGTACATGA ACTGAGGGGA CAGGATGTCC
 1081 CAGGCGAAGG GCAGGGGGCC ACCCTTGGTC ACCTTCAGCT TGGCGGTCTG GGTGCCCTCG
 1141 TAGGGGCGGC CCTCGCCCTC GCCCTCGATC TCGAACTCGT GGCCGTTCAC GGAGCCCTCC
 1201 ATGTGCACCT TGAAGCGCAT GAACTCCTTG ATGATGGCCA TGTTATCCTC CTCGCCCTTG
 1261 CTCACCATCA ATCGATGTCC GGCGATCGCT GGCCGGCCTT TAATTAATGG CGCGCCACTA
 1321 GTGGATCCTG CAAAAAGAAC AAGTAGCTTG TATTCTATAG TGTCACCTAA ATCAAGCTTG
 1381 CTCCGACGTC CCCAGGCAGA ATGGCGGTTC CCTAAACGAG CATTGCTTAT ATAGACCTCC
 1441 CATTAGGCAC GCCTACCGCC CATTTACGTC AATGGAACGC CCATTTGCGT CATTGCCCCT
 1501 CCCCATTGAC GTCAATGGGG ATGTACTTGG CAGCCATCGC GGGCCATTTA CCGCCATTGA
 1561 CGTCAATGGG AGTACTGCCA ATGTACCCTG GCGTACTTCC AATAGTAATG TACTTGCCAA
 1621 GTTACTATTA ATAGATATTG ATGTACTGCC AAGTGGGCCA TTTACCGTCA TTGACGTCAA
 1681 TAGGGGGCGT GAGAACGGAT ATGAATGGGC AATGAGCCAT CCCATTGACG TCAATGGTGG
 1741 GTGGTCCTAT TGACGTCAAT GGGCATTGAG CCAGGCGGGC CATTTACCGT AATTGACGTC
 1801 AATGGGGGAG GCGCCATATA CGTCAATAGG ACCGCCCATA TGACGTCAAT AGGAAAGACC
 1861 ATATATAGAG ACCATTGACG TCAATGGGGG AGTGGCTATG GGCGGTATTA GGAAGCCCCA
 1921 TATATGGTAT ATGGGACCGC CCATTGGGAG GGGCTATCTA CGTCAATAGG AAAACCCATA
 1981 TATGGAATAC TATATGGCAT AGGGCCAATA CATAGTATTG AACCTGGCCA ATAGCCATAT
 2041 TGGCATAGGG CCATATTGGA TATTGCCTAT ATATTGATCC TGGCATATAG CCAATATGGC
 2101 CGCCATTATT GGCACCATGC CAATTCAATA TGGCGGACTT GGCACAGGGC CAAGACCCCT
 2161 CCTCAAACTT GGCACCGTGC CAAGTAGACC CCTCCCCAGT TGGCACAGTG CCAGGTCCGC
 2221 CATATTGAAT TGGCATGGTG CCAAGTCCGC CATATTGAAT TGGCTGGGGT CCAGATCCAC
 2281 CATATTGAAT TGGCACGAGT CCATATACGC CATATTGAAT TGGCACAGGT CCAGATCCAC
 2341 CATATTGAAT TGGCATGAGT CCATATACGC CATATTGAAT TGGCGGTCGA CTGGCGTAAT
 2401 AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG
 2461 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC
 2521 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC
 2581 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT
 2641 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG
 2701 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT
 2761 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA
 2821 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT
 2881 AACGCGAATT TTAACAAAAT ATTAACGCTT ACAATTTAGG TGGCACTTTT CGGGGAAATG
 2941 TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA
 3001 GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC
 3061 ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC
 3121 CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA
 3181 TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC
 3241 CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG
 3301 GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC
 3361 CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA
 3421 TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG
 3481 AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC
 3541 CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG
 3601 CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT
 3661 TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG
 3721 CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG
 3781 CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC
 3841 AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC
 3901 ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT
 3961 TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT
 4021 AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT
 4081 GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG
 4141 CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA
 4201 GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA
 4261 AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG
 4321 CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG
 4381 CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT
 4441 ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA
 4501 GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC
 4561 TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG
 4621 AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG
 4681 CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT
 4741 TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC
 4801 GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAG
//

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载体应用举例

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