| 出品公司: | Agilent |
|---|---|
| 载体名称: | pDEST R4-R3 Vector II |
| 载体又名: | pDEST R4-R3载体，pDEST R4-R3质粒，pDEST R4-R3载体质粒 |
| 英文名称: | pDEST R4-R3 Vector II, pDEST R4-R3 Vector, pDEST R4-R3 Vector II Plasmid |
| 质粒类型: | 哺乳动物细胞表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 6.8kb |
| 5’测序引物: | -- |
| 3’测序引物: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 克隆菌株: | DH5α,TOP10 等 |
| 备注: | |
| 产品目录号: | 12537 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pDEST R4-R3 Vector II载体质粒基本信息
pDEST R4-R3 Vector II质粒图谱载体图谱和pDEST R4-R3 Vector II载体序列质粒序列多克隆位点信息
pDEST R4-R3 Vector II质粒载体简介
pDEST R4-R3 Vector II质粒序列载体序列
LOCUS pDEST R4-R3 4107 bp DNA circular SYN 07-MAR-2024
DEFINITION Gateway(R) destination vector for generating an expression clone by
three-fragment multisite recombination.
ACCESSION .
VERSION .
KEYWORDS pDEST R4-R3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4107)
AUTHORS Thermo Fisher (Invitrogen)
TITLE Direct Submission
JOURNAL Exported Jun 6, 2025 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4107
/lab_host="Escherichia coli"
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 1..17
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 36..160
/gene="mutant version of attR"
/label=attR4
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
CDS complement(201..506)
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
CDS complement(848..1507)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
promoter complement(1561..1591)
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
protein_bind complement(1617..1740)
/gene="mutant version of attR"
/label=attR3
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
primer_bind complement(1748..1764)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 2238..2342
/gene="bla"
/label=AmpR promoter
CDS 2343..3203
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3374..3962
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 caggaaacag ctatgaccat gattacgcca agctatcaac tttgtataga aaagttgaac
61 gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga
121 ctacataata ctgtaaaaca caacatatcc agtcactatg gtcgacctgc agactggctg
181 tgtataaggg agcctgacat ttatattccc cagaacatca ggttaatggc gtttttgatg
241 tcattttcgc ggtggctgag atcagccact tcttccccga taacggagac cggcacactg
301 gccatatcgg tggtcatcat gcgccagctt tcatccccga tatgcaccac cgggtaaagt
361 tcacggggga ctttatctga cagcagacgt gcactggcca gggggatcac catccgtcgc
421 ccgggcgtgt caataatatc actctgtaca tccacaaaca gacgataacg gctctctctt
481 ttataggtgt aaaccttaaa ctgcatttca ccagcccctg ttctcgtcgg caaaagagcc
541 gttcatttca ataaaccggg cgacctcagc catcccttcc tgattttccg ctttccagcg
601 ttcggcacgc agacgacggg cttcattctg catggttgtg cttaccgaac cggagatatt
661 gacatcatat atgccttgag caactgatag ctgtcgctgt caactgtcac tgtaatacgc
721 tgcttcatag catacctctt tttgacatac ttcgggtata catatcagta tatattctta
781 taccgcaaaa atcagcgcgc aaatacgcat actgttatct ggcttttagt aagccggatc
841 ctctagatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
901 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
961 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
1021 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
1081 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
1141 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
1201 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
1261 ccagctcacc gtctttcatt gccatacgga attccggatg agcattcatc aggcgggcaa
1321 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
1381 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
1441 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
1501 tctccatttt agcttcctta gctcctgaaa atctcgacgg atcctaactc aaaatccaca
1561 cattatacga gccggaagca taaagtgtaa agcctggggg tgcctaatgc ggccgccata
1621 gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat
1681 ttaatatatt gatatttata tcattttacg tttctcgttc aactttatta tacatagttg
1741 ataattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
1801 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
1861 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt
1921 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
1981 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
2041 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
2101 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
2161 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
2221 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
2281 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
2341 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
2401 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
2461 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
2521 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
2581 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
2641 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
2701 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
2761 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
2821 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
2881 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
2941 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
3001 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
3061 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
3121 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
3181 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
3241 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
3301 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
3361 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
3421 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
3481 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
3541 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
3601 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
3661 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
3721 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
3781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
3841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
3901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
3961 aaaacgccag caacgcggcc tttttacggt tcctggtctt ttgctggcct tttgctcaca
4021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
4081 ctgataccag cggataacaa tttcaca
//
pDEST R4-R3 Vector II载体图谱质粒图谱pdf版和pDEST R4-R3 Vector II载体序列质粒序列等相关资料下载
pDEST R4-R3 Vector II质粒载体应用举例
- 上一篇：pDONR221/Zeo
- 下一篇：没有了
