YIplac128

价格:1000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: ATCC
载体名称: YIplac128
质粒类型: 酿酒酵母表达载体;酵母整合型载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: lacZ
载体大小: 4302 bp
5' 测序引物及序列: M13 Reverse:CAGGAAACAGCTATGAC
3' 测序引物及序列: M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: LEU2
表达菌株: 酿酒酵母、白色念珠菌
备注: --
产品目录号: #87592
稳定性: 稳表达
组成型/诱导型: 诱导型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

YIplac128载体图谱



YIplac128 多克隆位点

载体简介

载体序列

LOCUS       YIplac128	4302 bp 	DNA	SYN
DEFINITION  YIplac128
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="YIplac128"
                     /mol_type="other DNA"
     promoter        142..171
                     /label="lac_promoter"
     misc_feature    185..207
                     /label="M13_pUC_rev_primer"
     promoter        206..224
                     /label="M13_reverse_primer"
     misc_feature    287..442
                     /label="lacZ_a"
     promoter        complement(290..306)
                     /label="M13_forward20_primer"
     misc_feature    complement(299..321)
                     /label="M13_pUC_fwd_primer"
     misc_feature    634..656
                     /label="pGEX_3_primer"
     gene            complement(797..1891)
                     /label="LEU2 (variant)"
                     /gene="LEU2 (variant)"
     CDS             complement(797..1903)
                     /label="ORF frame 3"
                     /translation="MSAPMSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFD
                     FENHLIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRK
                     ELQLYANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVA
                     WDSEQYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEF
                     PTLKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLA
                     SLPDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVK
                     KVLDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA*"
     promoter        2431..2459
                     /label="AmpR_promoter"
                     /translation="MSAPMSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFD
                     FENHLIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRK
                     ELQLYANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVA
                     WDSEQYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEF
                     PTLKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLA
                     SLPDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVK
                     KVLDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA*"
     gene            2501..3361
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MSAPMSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFD
                     FENHLIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRK
                     ELQLYANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVA
                     WDSEQYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEF
                     PTLKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLA
                     SLPDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVK
                     KVLDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA*"
     CDS             2501..3361
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      3516..4135
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
   61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
  121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
  181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
  241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg
  301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
  361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
  421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
  481 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
  541 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
  601 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
  661 caccgtcatc accgaaacgc gcgagacgaa agggcctacc ctatgaacat attccatttt
  721 gtaatttcgt gtcgtttcta ttatgaattt catttataaa gtttatgtac aaatatcata
  781 aaaaaagaga atctttttaa gcaaggattt tcttaacttc ttcggcgaca gcatcaccga
  841 cttcggtggt actgttggaa ccacctaaat caccagttct gatacctgca tccaaaacct
  901 ttttaactgc atcttcaatg gccttacctt cttcaggcaa gttcaatgac aatttcaaca
  961 tcattgcagc agacaagata gtggcgatag ggtcaacctt attctttggc aaatctggag
 1021 cagaaccgtg gcatggttcg tacaaaccaa atgcggtgtt cttgtctggc aaagaggcca
 1081 aggacgcaga tggcaacaaa cccaaggaac ctgggataac ggaggcttca tcggagatga
 1141 tatcaccaaa catgttgctg gtgattataa taccatttag gtgggttggg ttcttaacta
 1201 ggatcatggc ggcagaatca atcaattgat gttgaacctt caatgtagga aattcgttct
 1261 tgatggtttc ctccacagtt tttctccata atcttgaaga ggccaaaaca ttagctttat
 1321 ccaaggacca aataggcaat ggtggctcat gttgtagggc catgaaagcg gccattcttg
 1381 tgattctttg cacttctgga acggtgtatt gttcactatc ccaagcgaca ccatcaccat
 1441 cgtcttcctt tctcttacca aagtaaatac ctcccactaa ttctctgaca acaacgaagt
 1501 cagtaccttt agcaaattgt ggcttgattg gagataagtc taaaagagag tcggatgcaa
 1561 agttacatgg tcttaagttg gcgtacaatt gaagttcttt acggattttt agtaaacctt
 1621 gttcaggtct aacactacct gtaccccatt taggaccacc cacagcacct aacaaaacgg
 1681 catcaacctt cttggaggct tccagcgcct catctggaag tgggacacct gtagcatcga
 1741 tagcagcacc accaattaaa tgattttcga aatcgaactt gacattggaa cgaacatcag
 1801 aaatagcttt aagaacctta atggcttcgg ctgtgatttc ttgaccaacg tggtcacctg
 1861 gcaaaacgac gatcttctta ggggcagaca taggggcaga cattagaatg gtatatcctt
 1921 gaaatatata tatatattgc tgaaatgtaa aaggtaagaa aagttagaaa gtaagacgat
 1981 tgctaaccac ctattggaaa aaacaatagg tccttaaata atattgtcaa cttcaagtat
 2041 tgtgatgcaa gcatttagtc atgaacgctt ctctattcta tatgaaaagc cggttccggc
 2101 ctctcacctt tcctttttct cccaattttt cagttgaaaa aggtatatgc gtcaggcgac
 2161 ctctgaaatt aacaaaaaat ttccagtcat cgaatttgat tctgtgcgat agcgcccctg
 2221 tgtgttctcg ttatgttgag gaaaaaaata atggttgcta agagattcga actcttgcat
 2281 cttacgatac ctgagtattc ccacagttgg cctcgtgata cgcctatttt tataggttaa
 2341 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
 2401 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
 2461 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
 2521 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
 2581 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
 2641 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
 2701 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
 2761 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
 2821 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
 2881 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
 2941 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
 3001 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
 3061 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
 3121 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
 3181 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
 3241 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
 3301 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
 3361 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
 3421 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
 3481 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
 3541 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
 3601 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
 3661 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
 3721 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
 3781 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
 3841 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
 3901 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
 3961 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
 4021 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
 4081 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
 4141 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
 4201 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
 4261 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa ga
//

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