pAAVLINK-3'-HIVEP2-FLAG-bGHpA

LOCUS       pAAVLINK-3'-HIVEP2-FLAG-bGHpA                7386 bp DNA     circular SYN 19-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7386)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 2, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     protein_bind    145..178
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          179..270
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             271..4326
                     /codon_start=1
                     /label=HIVEP2
                     /translation="GEVGMDQSVKQEQLEHLHAGLRSGWHHGPPAVLPPLQQEDPGKQV
                     AGPCPPLSSGPLHLAQPQIMHMDSQESLRNPLIQPTSYMTSKHLPEQPHLFPHQETIPF
                     SPIQNALFQFQYPTVCMVHLPAQQPPWWQAHFPHPFAQHPQKSYGKPSFQTEIHSSYPL
                     EHVAEHTGKKPAEYAHTKEQTYPCYSGASGLHPKNLLPKFPSDQSSKSTETPSEQVLQE
                     DFASANAGSLQSLPGTVVPVRIQTHVPSYGSVMYTSISQILGQNSPAIVICKVDENMTQ
                     RTLVTNAAMQGIGYNIAQVLGQHAGLEKYPIWKAPQTLPLGLESSIPLCLPSTSDSVAT
                     LGGSKRMLSPASSLELFMETKQQKRVKEEKMYGQIVEELSAVELTNSDIKKDLSRPQKP
                     QLVRQGCASEPKDGLQSGSSSFSSLSPSSSQDYPSVSPSSREPFPPSKEMLSGSRAPLP
                     GQKSSGPSESKESSDELDIDETASDMSMSPQSSSLPAGDGQLEEEGKGHKRPVGMLVRM
                     ASAPSGNVADSTLLLTDMADFQQILQFPSLRTTTTVSWCFLNYTKPNYVQQATFKSSVY
                     ASWCISSCNPNPSGLNTKTTLALLRSKQKITAEIYTLAAMHRPGTGKLTSSSAWKQFTQ
                     MKPDASFLFGSKLERKLVGNILKERGKGDIHGDKDIGSKQTEPIRIKIFEGGYKSNEDY
                     VYVRGRGRGKYICEECGIRCKKPSMLKKHIRTHTDVRPYVCKLCNFAFKTKGNLTKHMK
                     SKAHMKKCLELGVSMTSVDDTETEEAENLEDLHKAAEKHSMSSISTDHQFSDAEESDGE
                     DGDDNDDDDEDEDDFDDQGDLTPKTRSRSTSPQPPRFSSLPVNVGAVPHGVPSDSSLGH
                     SSLISYLVTLPSIRVTQLMTPSDSCEDTQMTEYQRLFQSKSTDSEPDKDRLDIPSCMDE
                     ECMLPSEPSSSPRDFSPSSHHSSPGYDSSPCRDNSPKRYLIPKGDLSPRRHLSPRRDLS
                     PMRHLSPRKEAALRREMSQRDVSPRRHLSPRRPVSPGKDITARRDLSPRRERRYMTTIR
                     APSPRRALYHNPPLSMGQYLQAEPIVLGPPNLRRGLPQVPYFSLYGDQEGAYEHPGSSL
                     FPEGPNDYVFSHLPLHSQQQVRAPIPMVPVGGIQMVHSMPPALSSLHPSPTLPLPMEGF
                     EEKKGASGESFSKDPYVLSKQHEKRGPHALQSSGPPSTPSSPRLLMKQSTSEDSLNATE
                     REQEENIQTCTKAIASLRIATEEAALLGPDQPARVQEPHQNPLGSAHVSIRHFSRPEPG
                     QPCTSATHPDLHDGEKDNFGTSQTPLAHSTFYSKSCVDDKQLDFHSSKELSSSTEESKD
                     PSSEKSQLH"
     misc_feature    370..375
                     /label=SDS
     misc_feature    1547..1552
                     /label=SDS
     misc_feature    2325..2330
                     /label=SDS
     misc_feature    2806..2811
                     /label=SDS
     misc_feature    2819..2824
                     /label=SDS
     misc_feature    3328..3333
                     /label=SDS
     misc_feature    3499..3504
                     /label=SDS
     misc_feature    3884..3889
                     /label=SDS
     misc_feature    3953..3958
                     /label=SDS
     CDS             4333..4398
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    4402..4626
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4649..4789
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4649..4778
                     /label=AAV2 ITR
     rep_origin      4864..5319
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5601..5705
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5706..6566
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6737..7325
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtataact tcgtatagga tactttatag caatttatga
      181 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
      241 ctgacatcca ctttgccttt ctctccacag ggcgaggttg gcatggatca gagcgtgaag
      301 caagagcagc tggagcacct gcatgctggc ctccggtccg ggtggcacca tggcccgcct
      361 gctgtgctgc ctcctcttca gcaagaggac ccagggaagc aggtggcggg tccttgtccc
      421 ccgctgagct cggggccact gcacctggcc cagccacaga tcatgcacat ggacagtcag
      481 gaatctttga gaaatccctt gatccaacca acatcctata tgacaagcaa gcacttacct
      541 gaacagccac acttatttcc acatcaagag acaattccat tttctccaat ccagaatgcc
      601 ttgtttcagt ttcagtatcc tacagtttgt atggttcatt taccagctca gcagcctccc
      661 tggtggcagg cacatttccc acatcccttt gctcagcacc ctcagaagag ctatggcaag
      721 ccctcttttc agacagaaat ccattcgagc tatcccttag agcatgtggc agagcacact
      781 ggaaagaaac ctgctgagta tgcacacacg aaagagcaga cctacccatg ttattcagga
      841 gcatcagggc tacacccaaa gaaccttctt ccaaagtttc catcagacca gagcagtaag
      901 tcaactgaaa cgccctctga gcaggttctt caagaagatt ttgcctcggc aaatgctggg
      961 tctttgcagt ccctcccagg aacagtggtt cctgttcgga tccagacgca cgtaccatcc
     1021 tatggaagtg tcatgtacac aagcatttct cagatacttg ggcagaatag ccctgccatt
     1081 gtcatatgca aagtcgatga gaatatgacc caaaggacac tggtcaccaa cgcagccatg
     1141 caagggatag gatacaacat tgcccaggtg ctggggcagc atgcgggctt ggagaagtac
     1201 cccatttgga aagcacctca gactttgccc ctcggcttag aatcctccat ccccttgtgt
     1261 ttaccttcca cctctgacag cgtggccacc ctgggaggta gcaagcgaat gctttctcca
     1321 gccagtagct tggagctctt catggaaacc aagcagcaga aaagggtcaa agaagaaaag
     1381 atgtacggac agattgtgga ggagcttagt gctgtggagc tgaccaactc agacatcaaa
     1441 aaggacctct cccgccccca gaaaccccag ctggttcgac aaggatgtgc ttctgagcca
     1501 aaagatggct tgcagtcagg gtcatcttcc ttctcctcgc tgtcgccctc ctcatctcaa
     1561 gactatcctt ctgttagccc gtcttccagg gagccattcc cgcccagcaa ggagatgctt
     1621 tccggttccc gggcaccact tccggggcag aagtccagtg ggccttctga aagcaaagaa
     1681 tcttcagatg aattagatat cgatgagacg gcatcggaca tgagcatgag cccacagagt
     1741 tcttcattac cagcaggaga tggtcagctg gaagaggaag ggaagggcca caagcggcct
     1801 gttggcatgc tggtccgcat ggcctctgcc cccagcggga acgtggcaga ctcaactctt
     1861 cttctcacgg acatggcaga tttccagcag attcttcagt tccccagtct gcggacaaca
     1921 actactgtga gttggtgctt cttgaattat acaaaaccca attatgtgca acaggccacc
     1981 ttcaaatcct cggtttatgc ttcatggtgc attagttcct gtaatccaaa cccatcagga
     2041 ttgaacacca agaccacgct ggctcttctg aggtccaagc aaaaaatcac tgcagaaatt
     2101 tatactctgg ctgctatgca taggcctgga accggcaagc ttacatcatc aagtgcttgg
     2161 aagcagttta ctcagatgaa acctgatgcg tcctttttat ttggcagcaa actagaaagg
     2221 aaactagtgg gaaatatctt aaaggaaaga gggaaaggag atattcatgg agataaagat
     2281 attggatcca aacaaactga gccaatccga attaaaatat ttgaaggagg gtacaaatcg
     2341 aatgaagatt atgtatatgt cagaggacgt ggccggggaa agtacatttg tgaagaatgt
     2401 gggattcgct gtaagaagcc aagcatgctc aaaaaacaca tccgtaccca tactgatgtt
     2461 cggccttatg tatgcaagtt atgtaacttt gccttcaaaa cgaaaggaaa cctaacgaag
     2521 catatgaaat ctaaagcaca catgaaaaaa tgcctggaat tgggagtctc aatgacatcg
     2581 gtggatgata cagaaactga ggaagcagaa aatttggaag atttgcacaa agcagcagag
     2641 aagcatagca tgtccagcat ttcaactgat catcagttct ccgatgctga ggaatcagat
     2701 ggtgaggatg gagatgataa tgatgatgat gatgaagatg aagatgactt tgacgaccag
     2761 ggagatttaa caccaaaaac aagatcaaga agcaccagtc ctcagcctcc tagattctcc
     2821 tccttgcctg tgaatgttgg cgccgtaccc cacggggttc cttcagatag ttccctggga
     2881 cattcttcgt tgatcagcta tttggttact ttgccaagta ttcgagttac tcagcttatg
     2941 acacccagtg attcatgtga agatacccag atgacagaat accagaggct attccagagc
     3001 aaaagtacgg actcagaacc agacaaagac agattggaca tacctagttg tatggatgag
     3061 gagtgcatgc taccttcaga gccaagctcc tctcccaggg acttctcacc ctcaagccac
     3121 cattcctctc caggatatga ttcttcaccc tgtcgagata attcaccaaa gaggtatctg
     3181 atacccaaag gagatttatc tcccaggaga catttatcac ctaggagaga tctgtcaccc
     3241 atgagacatc tttcaccaag aaaggaagct gcattgagaa gagagatgtc ccaaagagat
     3301 gtttcaccaa gaaggcattt gtctccaagg aggccagtgt ctcctgggaa agatatcaca
     3361 gcaagaagag acctctctcc tagaagagag agaagataca tgaccacaat aagagcgcca
     3421 tctcccagaa gggctttata ccataaccca ccattgtcca tgggacagta tttgcaagca
     3481 gagccaattg tattggggcc tcctaattta agaagaggat tacctcaggt tccttacttc
     3541 agtctctatg gagaccaaga aggtgcttat gaacatccag gctccagcct tttccctgag
     3601 ggtcctaatg actatgtctt cagtcatctt ccactccact ctcagcaaca agtgcgagcc
     3661 cctatcccca tggtgcccgt tggtgggatc cagatggttc actccatgcc gccagccctt
     3721 tccagtttac atccttcacc cacattgccc ctgccaatgg agggctttga ggagaagaaa
     3781 ggcgcgtcag gggagtcctt ctccaaggac ccctatgtgc tttctaagca gcatgagaag
     3841 cgaggtcctc acgctttgca gtcatctggt ccgcctagca ctccctcctc tcctcggctg
     3901 ttgatgaaac agagcacttc ggaagacagc ctaaacgcaa cagagcggga acaggaggaa
     3961 aatatacaga cttgtacaaa agccattgcc tctctccgga ttgccacgga agaggcagct
     4021 ctgctcgggc cagatcagcc agcgcgggtg caggagcccc accagaaccc cctgggaagt
     4081 gcacatgtta gcattagaca ctttagtaga cctgagccag gtcagccctg tacctcagcc
     4141 acccaccctg acttgcatga tggtgaaaag gacaattttg gtacatcaca gactccatta
     4201 gctcactcca cgttttacag caagagttgt gtggatgaca agcagttgga ctttcacagc
     4261 agcaaggaat tatcttcaag cacagaggaa agcaaagatc cttcatcaga aaagagtcag
     4321 ctacattgta cagactacaa agaccatgac ggtgattata aagatcatga catcgactac
     4381 aaggatgacg atgacaagta actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     4441 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     4501 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     4561 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     4621 ctatggcacg tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc
     4681 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
     4741 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg
     4801 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
     4861 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4921 ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg
     4981 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     5041 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
     5101 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     5161 gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt
     5221 tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat
     5281 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
     5341 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     5401 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     5461 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     5521 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     5581 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5641 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     5701 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     5761 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     5821 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     5881 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     5941 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     6001 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     6061 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     6121 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     6181 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     6241 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     6301 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     6361 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     6421 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     6481 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     6541 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     6601 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6661 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6721 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6781 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6841 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     6901 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6961 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     7021 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7081 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     7141 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     7201 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     7261 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     7321 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     7381 acatgt
//