pIZ/V5-His

价格:1600元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Invitrogen
载体名称: pIZ/V5-His
质粒类型: 昆虫表达载体
克隆方法: 多克隆位点,限制性内切酶
启动子: OplE2
载体大小: 2876 bp
5' 测序引物及序列: OpIE2 Forward:CGCAACGATCTGGTAAACAC
3' 测序引物及序列: 5´-GACAATACAAACTAAGATTTAGTCAG-3´
载体标签: V5 Epitope Tag , His Tag (C-末端)
载体抗性: Zeocin
真核筛选标记: Zeocin
克隆菌株: TOP10;DH5
宿主细胞(系): Sf9, Sf21,High Five
备注: --
产品目录号: V8000-01
瞬表达/稳表达: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pIZ-V5-His载体图谱



pIZ-V5-His 多克隆位点

pIZ-V5-His 载体特征

载体简介

载体序列

LOCUS       pIZ/V5-His	2876 bp 	DNA	SYN
DEFINITION  pIZ/V5-His
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="pIZ/V5-His"
                     /mol_type="other DNA"
     misc_feature    515..534
                     /label="OpIE2_fwd_primer"
     rep_origin      complement(991..1610)
                     /label="pBR322_origin"
     misc_feature    2176..2195
                     /label="OpIE2_fwd_primer"
     promoter        2281..2299
                     /label="T7_promoter"
     gene            2309..2680
                     /label="bleo"
                     /gene="bleo"
     misc_feature    2309..2683
                     /label="sh_ble"
     terminator      2750..5
                     /label="SV40_PA_terminator"
     misc_feature    2838..2857
                     /label="EBV_rev_primer"
ORIGIN
    1 GGATCATGAT GATAAACAAT GTATGGTGCT AATGTTGCTT CAACAACAAT TCTGTTGAAC
   61 TGTGTTTTCA TGTTTGCCAA CAAGCACCTT TATACTCGGT GGCCTCCCCA CCACCAACTT
  121 TTTTGCACTG CAAAAAAACA CGCTTTTGCA CGCGGGCCCA TACATAGTAC AAACTCTACG
  181 TTTCGTAGAC TATTTTACAT AAATAGTCTA CACCGTTGTA TACGCTCCAA ATACACTACC
  241 ACACATTGAA CCTTTTTGCA GTGCAAAAAA GTACGTGTCG GCAGTCACGT AGGCCGGCCT
  301 TATCGGGTCG CGTCCTGTCA CGTACGAATC ACATTATCGG ACCGGACGAG TGTTGTCTTA
  361 TCGTGACAGG ACGCCAGCTT CCTGTGTTGC TAACCGCAGC CGGACGCAAC TCCTTATCGG
  421 AACAGGACGC GCCTCCATAT CAGCCGCGCG TTATCTCATG CGCGTGACCG GACACGAGGC
  481 GCCCGTCCCG CTTATCGCGC CTATAAATAC AGCCCGCAAC GATCTGGTAA ACACAGTTGA
  541 ACAGCATCTG TTCGAATTTA AAGCTTGGTA CCGAGCTCGG ATCCACTAGT CCAGTGTGGT
  601 GGAATTCTGC AGATATCCAG CACAGTGGCG GCCGCTCGAG TCTAGAGGGC CCGCGGTTCG
  661 AAGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGCGTACC GGTCATCATC
  721 ACCATCACCA TTGAGTTTAT CTGACTAAAT CTTAGTTTGT ATTGTCATGT TTTAATACAA
  781 TATGTTATGT TTAAATATGT TTTTAATAAA TTTTATAAAA TAATTTCAAC TTTTATTGTA
  841 ACAACATTGT CCATTTACAC ACTCCTTTCA AGCGCGTGGG ATCGATGCTC ACTCAAAGGC
  901 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG
  961 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG
 1021 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
 1081 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
 1141 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
 1201 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT
 1261 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
 1321 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
 1381 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
 1441 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
 1501 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
 1561 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG
 1621 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA TGATAAACAA
 1681 TGTATGGTGC TAATGTTGCT TCAACAACAA TTCTGTTGAA CTGTGTTTTC ATGTTTGCCA
 1741 ACAAGCACCT TTATACTCGG TGGCCTCCCC ACCACCAACT TTTTTGCACT GCAAAAAAAC
 1801 ACGCTTTTGC ACGCGGGCCC ATACATAGTA CAAACTCTAC GTTTCGTAGA CTATTTTACA
 1861 TAAATAGTCT ACACCGTTGT ATACGCTCCA AATACACTAC CACACATTGA ACCTTTTTGC
 1921 AGTGCAAAAA AGTACGTGTC GGCAGTCACG TAGGCCGGCC TTATCGGGTC GCGTCCTGTC
 1981 ACGTACGAAT CACATTATCG GACCGGACGA GTGTTGTCTT ATCGTGACAG GACGCCAGCT
 2041 TCCTGTGTTG CTAACCGCAG CCGGACGCAA CTCCTTATCG GAACAGGACG CGCCTCCATA
 2101 TCAGCCGCGC GTTATCTCAT GCGCGTGACC GGACACGAGG CGCCCGTCCC GCTTATCGCG
 2161 CCTATAAATA CAGCCCGCAA CGATCTGGTA AACACAGTTG AACAGCATCT GTTCGAATTA
 2221 ATTCGGATCT CTGCAGCACG TGTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA
 2281 TAATACGACT CACTATAGGA GGGCCACCAT GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT
 2341 CACCGCGCGC GACGTCGCCG GAGCGGTCGA GTTCTGGACC GACCGGCTCG GGTTCTCCCG
 2401 GGACTTCGTG GAGGACGACT TCGCCGGTGT GGTCCGGGAC GACGTGACCC TGTTCATCAG
 2461 CGCGGTCCAG GACCAGGTGG TGCCGGACAA CACCCTGGCC TGGGTGTGGG TGCGCGGCCT
 2521 GGACGAGCTG TACGCCGAGT GGTCGGAGGT CGTGTCCACG AACTTCCGGG ACGCCTCCGG
 2581 GCCGGCCATG ACCGAGATCG GCGAGCAGCC GTGGGGGCGG GAGTTCGCCC TGCGCGACCC
 2641 GGCCGGCAAC TGCGTGCACT TCGTGGCCGA GGAGCAGGAC TGACCGACGC CGACCAACAC
 2701 CGCCGGTCCG ACGGCGGCCC ACGGGTCCCA GGGGGGTCGA CCTCGAAACT TGTTTATTGC
 2761 AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT
 2821 TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCT

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