| 质粒名称: | pAAVLINK-EF1a-5'-COL2A1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001656 |
| 产品用途: | 5' AAVLINK plasmid for COL2A1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | COL2A1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-5'-COL2A1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-5'-COL2A1载体质粒基本信息
pAAVLINK-EF1a-5'-COL2A1质粒图谱载体图谱和pAAVLINK-EF1a-5'-COL2A1载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-5'-COL2A1质粒载体简介
pAAVLINK-EF1a-5'-COL2A1质粒序列载体序列
LOCUS pAAVLINK-EF1a-5'-COL2A1 6666 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6666)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 22, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6666
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 713..3806
/codon_start=1
/label=COL2A1
/translation="MIRLGAPQSLVLLTLLVAAVLRCQGQDVQEAGSCVQDGQRYNDKD
VWKPEPCRICVCDTGTVLCDDIICEDVKDCLSPEIPFGECCPICPTDLATASGQPGPKG
QKGEPGDIKDIVGPKGPPGPQGPAGEQGPRGDRGDKGEKGAPGPRGRDGEPGTPGNPGP
PGPPGPPGPPGLGGNFAAQMAGGFDEKAGGAQLGVMQGPMGPMGPRGPPGPAGAPGPQG
FQGNPGEPGEPGVSGPMGPRGPPGPPGKPGDDGEAGKPGKAGERGPPGPQGARGFPGTP
GLPGVKGHRGYPGLDGAKGEAGAPGVKGESGSPGENGSPGPMGPRGLPGERGRTGPAGA
AGARGNDGQPGPAGPPGPVGPAGGPGFPGAPGAKGEAGPTGARGPEGAQGPRGEPGTPG
SPGPAGASGNPGTDGIPGAKGSAGAPGIAGAPGFPGPRGPPGPQGATGPLGPKGQTGEP
GIAGFKGEQGPKGEPGPAGPQGAPGPAGEEGKRGARGEPGGVGPIGPPGERGAPGNRGF
PGQDGLAGPKGAPGERGPSGLAGPKGANGDPGRPGEPGLPGARGLTGRPGDAGPQGKVG
PSGAPGEDGRPGPPGPQGARGQPGVMGFPGPKGANGEPGKAGEKGLPGAPGLRGLPGKD
GETGAAGPPGPAGPAGERGEQGAPGPSGFQGLPGPPGPPGEGGKPGDQGVPGEAGAPGL
VGPRGERGFPGERGSPGAQGLQGPRGLPGTPGTDGPKGASGPAGPPGAQGPPGLQGMPG
ERGAAGIAGPKGDRGDVGEKGPEGAPGKDGGRGLTGPIGPPGPAGANGEKGEVGPPGPA
GSAGARGAPGERGETGPPGPAGFAGPPGADGQPGAKGEQGEAGQKGDAGAPGPQGPSGA
PGPQGPTGVTGPKGARGAQGPPGATGFPGAAGRVGPPGSNGNPGPPGPPGPSGKDGPKG
ARGDSGPPGRAGEPGLQGPAGPPGEKGEPGDDGPSGAEGPPGPQGLAGQRGIVGLPGQR
GERGFPGLPGPSGEPGKQGAPGASGDRGPPGPVGPPGLTGPA"
misc_feature 798..803
/label=SDS
misc_feature 1073..1078
/label=SDS
misc_feature 1442..1447
/label=SDS
misc_feature 2027..2032
/label=SDS
misc_feature 2720..2725
/label=SDS
misc_feature 3134..3139
/label=SDS
misc_feature 3224..3229
/label=SDS
misc_feature 3764..3769
/label=SDS
misc_feature 3782..3787
/label=SDS
intron 3807..3847
/label=Splicing Donor
misc_feature 3851..3884
/label=lox2272-JT1571
repeat_region 3929..4069
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3929..4058
/label=AAV2 ITR
rep_origin 4144..4599
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4881..4985
/gene="bla"
/label=AmpR promoter
CDS 4986..5846
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6017..6605
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgattcg
721 cctcggggct ccccagtcgc tggtgctgct gacgctgctc gtcgccgctg tccttcggtg
781 tcagggccag gatgtccagg aggctggcag ctgtgtgcag gatgggcaga ggtataatga
841 taaggatgtg tggaagccgg agccctgccg gatctgtgtc tgtgacactg ggactgtcct
901 ctgcgacgac ataatctgtg aagacgtgaa agactgcctc agccctgaga tccccttcgg
961 agagtgctgc cccatctgcc caactgacct cgccactgcc agtgggcaac caggaccaaa
1021 gggacagaaa ggagaacctg gagacatcaa ggatattgta ggacccaaag gacctcctgg
1081 gcctcaggga cctgcagggg aacaaggacc cagaggggat cgtggtgaca aaggtgaaaa
1141 aggtgcccct ggacctcgtg gcagagatgg agaacctggg acccctggaa atcctggccc
1201 ccctggtcct cccggccccc ctggtccccc tggtcttggt ggaaactttg ctgcccagat
1261 ggctggagga tttgatgaaa aggctggtgg cgcccagttg ggagtaatgc aaggaccaat
1321 gggccccatg ggacctcgag gacctccagg ccctgcaggt gctcctgggc ctcaaggatt
1381 tcaaggcaat cctggtgaac ctggtgaacc tggtgtctct ggtcccatgg gtccccgtgg
1441 tcctcctggt ccccctggaa agcctggtga tgatggtgaa gctggaaaac ctggaaaagc
1501 tggtgaaagg ggtccgcctg gtcctcaggg tgctcgtggt ttcccaggaa ccccaggcct
1561 tcctggtgtc aaaggtcaca gaggttatcc aggcctggac ggtgctaagg gagaggcggg
1621 tgctcctggt gtgaagggtg agagtggttc cccgggtgag aacggatctc cgggcccaat
1681 gggtcctcgt ggcctgcctg gtgaaagagg acggactggc cctgctggcg ctgcgggtgc
1741 ccgaggcaac gatggtcagc caggccccgc agggcctccg ggtcctgtcg gtcctgctgg
1801 tggtcctggc ttccctggtg ctcctggagc caagggtgaa gccggcccca ctggtgcccg
1861 tggtcctgaa ggtgctcaag gtcctcgcgg tgaacctggt actcctgggt cccctgggcc
1921 tgctggtgcc tccggtaacc ctggaacaga tggaattcct ggagccaaag gatctgctgg
1981 tgctcctggc attgctggtg ctcctggctt ccctgggcca cggggccctc ctggccctca
2041 aggtgcaact ggtcctctgg gcccgaaagg tcagacgggt gaacctggta ttgctggctt
2101 caaaggtgaa caaggcccca agggagaacc tggccctgct ggcccccagg gagcccctgg
2161 acccgctggt gaagaaggca agagaggtgc ccgtggagag cctggtggcg ttgggcccat
2221 cggtccccct ggagaaagag gtgctcccgg caaccgcggt ttcccaggtc aagatggtct
2281 ggcaggtccc aagggagccc ctggagagcg agggcccagt ggtcttgctg gccccaaggg
2341 agccaacggt gaccctggcc gtcctggaga acctggcctt cctggagccc ggggtctcac
2401 tggccgccct ggtgatgctg gtcctcaagg caaagttggc ccttctggag cccctggtga
2461 agatggtcgt cctggacctc caggtcctca gggggctcgt gggcagcctg gtgtcatggg
2521 tttccctggc cccaaaggtg ccaacggtga gcctggcaaa gctggtgaga agggactgcc
2581 tggtgctcct ggtctgaggg gtcttcctgg caaagatggt gagacaggtg ctgcaggacc
2641 ccctggccct gctggacctg ctggtgaacg aggcgagcag ggtgctcctg ggccatctgg
2701 gttccaggga cttcctggcc ctcctggtcc cccaggtgaa ggtggaaaac caggtgacca
2761 gggtgttccc ggtgaagctg gagcccctgg cctcgtgggt cccaggggtg aacgaggttt
2821 cccaggtgaa cgtggctctc ccggtgccca gggcctccag ggtccccgtg gcctccccgg
2881 cactcctggc actgatggtc ccaaaggtgc atctggccca gcaggccccc ctggggctca
2941 gggccctcca ggtcttcagg gaatgcctgg cgagagggga gcagctggta tcgctgggcc
3001 caaaggcgac aggggtgacg ttggtgagaa aggccctgag ggagcccctg gaaaggatgg
3061 tggacgaggc ctgacaggtc ccattggccc ccctggccca gctggtgcta atggcgagaa
3121 gggagaagtt ggacctcctg gtcctgcagg aagtgctggt gctcgtggcg ctccgggtga
3181 acgtggagag actgggcccc ccggaccagc gggatttgct gggcctcctg gtgctgatgg
3241 ccagcctggg gccaagggtg agcaaggaga ggccggccag aaaggcgatg ctggtgcccc
3301 tggtcctcag ggcccctctg gagcacctgg gcctcagggt cctactggag tgactggtcc
3361 taaaggagcc cgaggtgccc aaggcccccc gggagccact ggattccctg gagctgctgg
3421 ccgcgttgga cccccaggct ccaatggcaa ccctggaccc cctggtcccc ctggtccttc
3481 tggaaaagat ggtcccaaag gtgctcgagg agacagcggc ccccctggcc gagctggtga
3541 acccggcctc caaggtcctg ctggaccccc tggcgagaag ggagagcctg gagatgacgg
3601 tccctctggt gccgaaggtc caccaggtcc ccagggtctg gctggtcaga gaggcatcgt
3661 cggtctgcct gggcaacgtg gtgagagagg attccctggc ttgcctggcc cgtcgggtga
3721 gcccggcaag cagggtgctc ctggagcatc tggagacaga ggtcctcctg gccccgtggg
3781 tcctcctggc ctgacgggtc ctgcaggtaa gtatcaaggt tacaagacag gtttaaggag
3841 accaataagc tattattcgt ataggatact ttatacgaag ttattaagtt atggagaccg
3901 gtgtggcacg tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc
3961 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
4021 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg
4081 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
4141 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
4201 ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg
4261 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
4321 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
4381 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
4441 gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt
4501 tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat
4561 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
4621 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
4681 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
4741 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
4801 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
4861 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
4921 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
4981 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
5041 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
5101 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
5161 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
5221 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
5281 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
5341 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
5401 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
5461 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
5521 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
5581 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
5641 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
5701 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
5761 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
5821 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
5881 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
5941 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
6001 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
6061 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
6121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
6181 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
6241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
6301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
6361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
6421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
6481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
6541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
6601 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
6661 acatgt
//
