| 质粒名称: | pSR43.6r |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 63197 |
| 存储实验室: | Jeffrey Tabor |
|
载体骨架基本信息 |
|
| 载体名称: | pACYCDuet-1 |
| 载体抗性: | 大观霉素 |
| 完整质粒大小: | 6282 bp |
| 载体类型: | Bacterial Expression, Synthetic Biology |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | CcaS |
| 物种来源: | Synechocystis sp. PCC 6803 |
| 插入大小: | 2262 bp |
|
文献引用方法 |
|
| 材料和方法部分: | pSR43.6r was a gift from Jeffrey Tabor (爱迪基因 plasmid # 63197) |
| 参考文献部分: | Refactoring and optimization of light-switchable escherichia coli two-component systems. Schmidl SR, Sheth RU, Wu A, Tabor JJ. ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. Epub 2014 Oct 11. 10.1021/sb500273n PubMed 25250630 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pSR43.6r 6282 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION Expresses CcaS, Ho1, and PcyA constitutively.
ACCESSION .
VERSION .
KEYWORDS pSR43.6r
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6282)
AUTHORS Schmidl SR, Sheth RU, Wu A, Tabor JJ
TITLE Refactoring and optimization of light-switchable escherichia coli
two-component systems.
JOURNAL ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n.
Epub 2014 Oct 11.
PUBMED 25250630
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pSR43.6r|
FEATURES Location/Qualifiers
source 1..6282
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 2353..2424
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 2440..2467
/label=T7Te terminator
/note="phage T7 early transcription terminator"
promoter 2585..2618
/label=J23119 promoter
/note="bacterial promoter (Registry of Standard Biological
Parts BBa_J23119)"
RBS 2635..2646
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
RBS 3378..3389
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
terminator 4157..4228
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 4244..4271
/label=T7Te terminator
/note="phage T7 early transcription terminator"
CDS 4471..5262
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
terminator 5274..5368
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
rep_origin 5482..6027
/direction=RIGHT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
"
primer_bind 5928..5947
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 6144..6161
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 tttacggcta gctcagtcct aggtatagtg ctagctatag aggttaaaat cttaatagga
61 ggaaggcgca atgggcaaat ttctaattcc aatcgaattt gtttttctgg cgatcgccat
121 gacctgttat ttatggcaca gacaaaacca agaacgccgc aggattgaaa ttagcatcaa
181 gcaacaaacc caacgggaac gatttattaa ccaaattacc caacatatcc gccaatcttt
241 aaacttggaa acggttttaa ataccaccgt cgctgaagtt aaaaccctgt tgcaagttga
301 tcgagttcta atttatcgca tttggcaaga tggcacgggc agcgccatta cggaatcggt
361 gaatgccaat tatcctagta ttttagggcg gaccttttcc gatgaagttt ttcccgttga
421 ataccatcaa gcctacacca aaggtaaagt acgggccatt aatgacattg accaggatga
481 catagagatt tgcctagctg atttcgtcaa acaatttggc gtgaaatcaa aattagtagt
541 gcccattctt caacataatc gtgcttcttc cctagataat gaatcagaat ttccctatct
601 ttgggggctg ttaattaccc atcaatgtgc ttttacccgg ccatggcaac cgtgggaagt
661 ggagttaatg aaacagctag ccaatcaggt cgcgatcgcc atccaacaat cggaattata
721 tgagcaatta cagcaactca ataaagattt ggaaaaccga gtcgaaaaac gcacccagca
781 acttgccgcc accaatcaat ccctaagaat ggaaatcagt gagcgacaaa aaacggaagc
841 cgctctccgc cacactaacc atactctgca atccctgatt gcggcctccc ccaggggtat
901 ttttaccctt aatttagcag accaaattca gatttggaat cctacagcag aacgtatttt
961 tggttggaca gaaacagaaa ttattgccca tccagaatta ttaacatcca acattttgct
1021 ggaagattat cagcaattta aacagaaagt tttatcaggc atggtttccc ctagcctaga
1081 attaaaatgt caaaaaaaag atggtagttg gattgaaatt gtcctttccg ctgctcccct
1141 attggatagt gaagaaaata ttgccggatt ggtggcggtt gtcgccgata ttaccgagca
1201 aaagcggcag gcagaacaaa ttcgtttgct acaatccgtt gtggttaata ctaatgatgc
1261 ggtggtgatt acggaagcgg agcccattga tgatcccggg ccgagaattc tctatgtcaa
1321 tgaagcattt actaaaatca ccggttatac tgctgaagaa atgctaggca aaaccccccg
1381 agttttacag ggaccaaaaa ctagtcgcac tgaattagat agggtgcggc aagccattag
1441 tcaatggcaa tcagttaccg ttgaagtgat taattatcgt aaggatggca gtgagttttg
1501 ggtggaattt agtctggtgc ccgttgccaa taaaacaggt ttttacaccc attggattgc
1561 tgtgcaaagg gatgtcactg agcgccgacg cacggaggaa gtccgcctag ctttagaacg
1621 ggaaaaagaa ttaagccgcc taaaaactcg ttttttctcc atggcttccc atgaatttcg
1681 tactcccctc agtacggcct tagctgctgc ccaattactg gaaaattctg aagtggcctg
1741 gcttgatccc gataagcgta gccggaactt acaccgtatt caaaattccg tgaaaaatat
1801 ggtacagctc ctggatgata ttttaatcat taaccgtgcc gaagcgggca aattggaatt
1861 taatcctaat tggttagatt tgaaattatt gttccagcaa tttatcgaag aaattcaatt
1921 aagtgtcagt gaccaatatt attttgactt tatttgtagc gctcaagata cgaaggcatt
1981 ggtggatgaa aggttagtgc ggtctatttt atctaatctg ttatctaatg cgattaaata
2041 ctctcccggg ggagggcaga ttaaaattgc cctaagccta gattcggaac agattatttt
2101 tgaagtcacc gaccagggca ttggcatttc gccagaggac caaaagcaaa tttttgaacc
2161 ctttcatcgg ggcaaaaatg tcagaaatat tacgggaaca ggactcggtt taatggttgc
2221 caagaaatgt gttgacttac acagtggcag tatcttgcta aaaagtgcag ttgaccaggg
2281 aacaacagtt actatctgtt taaaacgcta taaccatttg cctcgagctt agtaataatc
2341 tagaccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
2401 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
2461 ttctgcgttt atagtaaatc actgcataat tcgtgtcgat taaaccgcct aagtccccca
2521 ggaaaggggg atataacagt atagattttg tcagccttca gcttggcttt accgtcaaaa
2581 aaactgacag ctagctcagt cctaggtata atgctagctc acacagaatt cattaaagag
2641 gagaaaggta ccatgagtgt caacttagct tcccagttgc gggaagggac gaaaaaatcc
2701 cactccatgg cggagaacgt cggctttgtc aaatgcttcc tcaagggcgt tgtcgagaaa
2761 aattcctacc gtaagctggt tggcaatctc tactttgtct acagtgccat ggaagaggaa
2821 atggcaaaat ttaaggacca tcccatcctc agccacattt acttccccga actcaaccgc
2881 aaacaaagcc tagagcaaga cctgcaattc tattacggct ccaactggcg gcaagaagtg
2941 aaaatttctg ccgctggcca agcctatgtg gaccgagtcc ggcaagtggc cgctacggcc
3001 cctgaattgt tggtggccca ttcctacacc cgttacctgg gggatctttc cggcggtcaa
3061 attctcaaga aaattgccca aaatgccatg aatctccacg atggtggcac agctttctat
3121 gaatttgccg acattgatga cgaaaaggct tttaaaaata cctaccgtca agctatgaat
3181 gatctgccca ttgaccaagc caccgccgaa cggattgtgg atgaagccaa tgacgccttt
3241 gccatgaaca tgaaaatgtt caacgaactt gaaggcaacc tgatcaaggc gatcggcatt
3301 atggtgttca acagcctcac ccgtcgccgc agtcaaggca gcaccgaagt tggcctcgcc
3361 acctccgaag gctagttaaa gaggagaaag gatccatggc cgtcactgat ttaagtttga
3421 ccaattcttc cctgatgccc acgttgaacc cgatgattca acagttggcc ctggcgatcg
3481 ccgctagttg gcaaagttta cccctcaagc cctatcaatt gccggaggat ttgggctacg
3541 tagaaggccg cctggaaggg gaaaagttag tgattgaaaa tcggtgctac caaacgcccc
3601 agtttcgcaa aatgcatttg gagttggcca aggtgggcaa agggttggat attctccact
3661 gtgtaatgtt tcctgagcct ttatacggtc tacctttgtt tggctgtgac attgtggccg
3721 gccccggtgg agtaagtgcg gctattgcgg atctatcccc cacccaaagc gatcgccaat
3781 tgcccgcagc gtaccaaaaa tcattggcag agctaggcca gccagaattt gagcaacaac
3841 gggaattgcc cccctgggga gaaatatttt ctgaatattg tttattcatc cgtcccagca
3901 atgtcactga agaagaaaga tttgtacaaa gggtagtgga ctttttgcaa attcattgtc
3961 accaatccat cgttgccgaa cccttgtctg aagctcaaac tttggagcac cgtcaggggc
4021 aaattcatta ctgccaacaa caacagaaaa atgataaaac ccgtcgggta ctggaaaaag
4081 cttttgggga agcttgggcg gaacggtata tgagccaagt cttatttgat gttatccaat
4141 aatctagacc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
4201 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
4261 gcctttctgc gtttataggt acccggggat cctctagagt cgacctgcag gcatgcaagc
4321 ttagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc
4381 aaagtaaact ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagatct
4441 gatcaagaga caggatgagg atcgtttcgc atgagggaag cggtgatcgc cgaagtatcg
4501 actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc
4561 gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg
4621 ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt
4681 ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt
4741 gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga
4801 gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat
4861 ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg
4921 gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta
4981 acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg
5041 tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac
5101 tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct
5161 tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc
5221 cactacgtga aaggcgagat caccaaggta gtcggcaaat aagagctcgc ttggactcct
5281 gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg
5341 ccgccgggcg ttttttattg gtgagaatcc aagcactagt aacaacttat atcgtatggg
5401 gctgacttca ggtgctacat ttgaagagat aaattgcact gaaatctaga aatattttat
5461 ctgattaata agatgatctt cttgagatcg ttttggtctg cgcgtaatct cttgctctga
5521 aaacgaaaaa accgccttgc agggcggttt ttcgaaggtt ctctgagcta ccaactcttt
5581 gaaccgaggt aactggcttg gaggagcgca gtcaccaaaa cttgtccttt cagtttagcc
5641 ttaaccggcg catgacttca agactaactc ctctaaatca attaccagtg gctgctgcca
5701 gtggtgcttt tgcatgtctt tccgggttgg actcaagacg atagttaccg gataaggcgc
5761 agcggtcgga ctgaacgggg ggttcgtgca tacagtccag cttggagcga actgcctacc
5821 cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc ataacagcgg aatgacaccg
5881 gtaaaccgaa aggcaggaac aggagagcgc acgagggagc cgccaggggg aaacgcctgg
5941 tatctttata gtcctgtcgg gtttcgccac cactgatttg agcgtcagat ttcgtgatgc
6001 ttgtcagggg ggcggagcct atggaaaaac ggctttgccg cggccctctc acttccctgt
6061 taagtatctt cctggcatct tccaggaaat ctccgccccg ttcgtaagcc atttccgctc
6121 gccgcagtcg aacgaccgag cgtagcgagt cagtgagcga ggaagcggaa tatatcccct
6181 aggtggagtc tggcctcaaa tacaaagtct gcaaagtctc gaaatattaa accagagaat
6241 aaataattaa acgccaaaat atttctagca aaaaccccag tt
//
