pLVX-Puro

价格:1500元

联系方式:I47-825O-882O

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pLVXpuro载体基本信息

出品公司: Clontech
载体名称: pLVX-Puro , pLVXpuro
质粒类型: 哺乳动物细胞慢病毒表达载体
高拷贝/低拷贝: 高拷贝
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 8102 bp
5' 测序引物及序列: CMV-F:CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄
筛选标记: 嘌呤霉素
备注: 含有组成型CMV启动子的慢病毒载体
产品目录号: 632164
稳定性: /
组成型: --
病毒/非病毒: 慢病毒

pLVXpuro载体质粒图谱和多克隆位点信息

pLVX-Puro载体图谱


pLVX-Puro多克隆位点

pLVXpuro载体简介

Description

pLVX-Puro is an HIV-1-based, lentiviral expression vector. Lentiviral particles derived from the vector allow you to express your gene of interest in virtually any cell type, even primary cells. Expression of your gene is driven by the constitutively active human cytomegalovirus immediate early promoter (PCMV IE), located just upstream of the multiple cloning site (MCS), allowing constitutive, high level expression of your protein of interest.

pLVX-Puro contains all of the viral processing elements necessary for the production of replication-incompetent lentivirus, as well as elements to improve viral titer, transgene expression, and overall vector function. The woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) promotes RNA processing events and enhances nuclear export of viral and transgene RNA (1), leading to increased viral titers from packaging cells, and enhanced expression of your gene of interest in target cells. In addition, the vector includes a Rev-response element (RRE), which further increases viral titers by enhancing the transport of unspliced viral RNA out of the nucleus (2). Finally, pLVX-Puro also contains a central polypurine tract (cPPT) element that increases nuclear importation of the viral genome during target cell infection, resulting in improved vector integration and more effi cient transduction (3). In addition to lentiviral elements, pLVX-Puro contains a puromycin resistance gene (Puror) under the control of the murine phosphoglycerate kinase (PGK) promoter (PPGK) for the selection of stable transductants. The vector also contains a pUC origin of replication and an E. coli ampicillin resistance gene (Ampr) for propagation and selection in bacteria.

Use
pLVX-Puro constitutively expresses your gene of interest from PCMV IE when transduced into target cells. Before the vector can be transduced into cells, however, it must be transfected into 293T packaging cells with our Lenti-X™ HT Packaging System (Cat. Nos. 632160 and 632161). This packaging system allows you to safely produce high titer, infectious, replication-incompetent, VSV-G pseudotyped lentiviral particles that can infect a wide range of cell types, including non-dividing and primary cells (4).

pLVXpuro载体序列

LOCUS       pLVX-Puro	8102 bp 	DNA     circular	SYN
DEFINITION  pLVX-Puro
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..8102
                     /organism="pLVX-Puro"
                     /mol_type="other DNA"
     misc_feature    454..634
                     /label="HIV-1_5_LTR"
     misc_feature    454..634
                     /label="truncHIV-1_3_LTR"
     misc_feature    745..789
                     /label="HIV-1_psi_pack"
     CDS             complement(1015..1476)
                     /label="ORF frame 3"
     CDS             1181..2032
                     /label="ORF frame 2"
     misc_feature    1303..1536
                     /label="RRE"
     misc_feature    2031..2046
                     /label="cPPT"
     promoter        2198..2702
                     /label="CMV_immearly_promoter"
     misc_feature    2205..2492
                     /label="CAG_enhancer"
     misc_feature    2659..2679
                     /label="CMV_fwd_primer"
     promoter        2660..2729
                     /label="CMV_promoter"
     misc_feature    2703..2722
                     /label="pCEP_fwd_primer"
     misc_feature    2705..2729
                     /label="LNCX_primer"
     misc_feature    complement(2925..2948)
                     /label="MSCV_rev_primer"
     CDS             3170..4009
                     /label="ORF frame 2"
     misc_feature    3322..3341
                     /label="mPGK_F_primer"
     gene            3410..4009
                     /label="puro (variant)"
                     /gene="puro (variant)"
     misc_feature    4025..4600
                     /label="WPRE"
     CDS             4113..4640
                     /label="ORF frame 3"
     misc_feature    5271..5451
                     /label="HIV-1_5_LTR"
     misc_feature    5271..5451
                     /label="truncHIV-1_3_LTR"
     promoter        complement(5576..5594)
                     /label="M13_reverse_primer"
     misc_feature    complement(5593..5615)
                     /label="M13_pUC_rev_primer"
     promoter        complement(5629..5658)
                     /label="lac_promoter"
     gene            complement(6741..7601)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(6741..7601)
                     /label="ORF frame 1"
     promoter        complement(7643..7671)
                     /label="AmpR_promoter"
     terminator      7757..7888
                     /label="SV40_PA_terminator"
     misc_feature    7845..7864
                     /label="EBV_rev_primer"
ORIGIN
    1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
   61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
  121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
  181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
  241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
  301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
  361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
  421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
  481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
  541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
  601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
  661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
  721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
  781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
  841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
  901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
  961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
 2821 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatcccgc gactctagat
 2881 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
 2941 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
 3001 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
 3061 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
 3121 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
 3181 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
 3241 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc
 3301 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt
 3361 tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta
 3421 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
 3481 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
 3541 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
 3601 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
 3661 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
 3721 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
 3781 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
 3841 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
 3901 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
 3961 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac cgcgtctgga
 4021 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
 4081 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
 4141 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
 4201 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
 4261 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
 4321 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
 4381 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
 4441 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
 4501 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
 4561 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta
 4621 attctgcagt cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
 4681 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
 4741 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
 4801 aaaagaaaag aggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga
 4861 tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca caccagggcc
 4921 aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga
 4981 taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca
 5041 tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt
 5101 tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg
 5161 ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga
 5221 gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct
 5281 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
 5341 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
 5401 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
 5461 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
 5521 ggccttgaca ttgctagcgt tttaccgtcg acctctagct agagcttggc gtaatcatgg
 5581 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
 5641 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
 5701 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
 5761 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
 5821 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
 5881 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
 5941 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
 6001 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
 6061 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
 6121 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
 6181 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
 6241 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
 6301 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
 6361 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
 6421 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
 6481 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
 6541 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
 6601 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
 6661 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
 6721 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
 6781 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
 6841 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
 6901 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
 6961 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
 7021 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
 7081 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
 7141 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
 7201 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
 7261 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
 7321 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
 7381 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
 7441 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
 7501 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
 7561 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
 7621 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
 7681 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
 7741 ggatcgggag atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
 7801 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
 7861 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
 7921 caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta
 7981 aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac
 8041 tacaaactta gtagttttta aagaaattgt atttgttaaa tatgtactac aaacttagta
 8101 gt
//

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