pCMV-SPORT6

价格:2500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Invitrigen
载体名称: pCMV-SPORT6
质粒类型: 哺乳细胞表达载体
高拷贝/低拷贝: 高拷贝
启动子: CMV
克隆方法: Gateway
载体大小: 4396
5' 测序引物及序列: Sp6 5'd[GATTTAGGTGACACTATAG]3'
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素(Ampicillin)
筛选标记: --
备注: pCMV-SPORT6载体用于构建cDNA文库。
产品目录号: 18248-013 
稳定性: 瞬表达
组成型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pCMV-SPORT6载体图谱



pCMV-SPORT6多克隆位点

载体简介

SuperScript质粒系统设计用于从纯化的 mRNA 群合成双链 cDNA。 cDNA 适合通过定向克隆导入 pSPORT1 载体或 pCMV-SPORT6 质粒载体中,随后可生成 cDNA 文库并使用 Gateway 技术。 SuperScript质粒系统:

• cDNA 产量更大,并且用 SuperScriptII RT 增加全长 cDNA

• 为定向克隆提供简化的引物-接头策略

• 为第一和第二链反应提供一管模式,提高 cDNA 产量

• 使用高效方便的预填充柱分离 cDNA

• 将插入片段连接进预先剪切的 Not I-Sal I 载体,这是为最大限度降低背景而制备的载体。

载体序列

LOCUS       pCMV-Sport 6	4396 bp 	DNA	SYN
DEFINITION  pCMV-Sport 6
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="pCMV-Sport 6"
                     /mol_type="other DNA"
     promoter        16..34
                     /label="T7_promoter"
     misc_feature    complement(36..60)
                     /label="attB2"
     misc_feature    complement(153..177)
                     /label="attB1"
     misc_feature    complement(179..196)
                     /label="Sp6_primer"
     misc_feature    complement(220..242)
                     /label="M13_pUC_rev_primer"
     promoter        complement(266..385)
                     /label="CMV2_promoter"
     promoter        complement(328..399)
                     /label="CMV_promoter"
     misc_feature    complement(328..352)
                     /label="LNCX_primer"
     misc_feature    complement(335..354)
                     /label="pCEP_fwd_primer"
     promoter        complement(351..855)
                     /label="CMV_immearly_promoter"
     misc_feature    complement(378..398)
                     /label="CMV_fwd_primer"
     misc_feature    complement(564..851)
                     /label="CAG_enhancer"
     promoter        1001..1029
                     /label="AmpR_promoter"
     rep_origin      complement(1301..1920)
                     /label="pBR322_origin"
     misc_feature    1982..2015
                     /label="loxP"
     gene            complement(2117..2977)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(2117..2977)
                     /label="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      complement(3397..3703)
                     /label="f1_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     terminator      complement(3856..3975)
                     /label="SV40_PA_terminator"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    complement(3870..3889)
                     /label="EBV_rev_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    complement(4263..4278)
                     /label="SV40_int"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    4370..4392
                     /label="M13_pUC_fwd_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        4385..5
                     /label="M13_forward20_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 CCAGTGCCTA GCTTATAATA CGACTCACTA TAGGGACCAC TTTGTACAAG AAAGCTGGGT
   61 ACGCGTAAGC TTGGGCCCCT CGAGGGATAC TCTAGAGCGG CCGCCGACTA GTGAGCTCGT
  121 CGACGATATC CCGGGAATTC CGGACCGGTA CCAGCCTGCT TTTTTGTACA AACTTGTTCT
  181 ATAGTGTCAC CTAAATAGGC CTAATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG
  241 CTCCGCGGCC TAGGCTAGAG TCCGGAGGCT GGATCGGTCC CGGTGTCTTC TATGGAGGTC
  301 AAAACAGCGT GGATGGCGTC TCCAGGCGAT CTGACGGTTC ACTAAACGAG CTCTGCTTAT
  361 ATAGACCTCC CACCGTACAC GCCTACCGCC CATTTGCGTC AATGGGGCGG AGTTGTTACG
  421 ACATTTTGGA AAGTCCCGTT GATTTTGGTG CCAAAACAAA CTCCCATTGA CGTCAATGGG
  481 GTGGAGACTT GGAAATCCCC GTGAGTCAAA CCGCTATCCA CGCCCATTGA TGTACTGCCA
  541 AAACCGCATC ACCATGGTAA TAGCGATGAC TAATACGTAG ATGTACTGCC AAGTAGGAAA
  601 GTCCCATAAG GTCATGTACT GGGCATAATG CCAGGCGGGC CATTTACCGT CATTGACGTC
  661 AATAGGGGGC GTACTTGGCA TATGATACAC TTGATGTACT GCCAAGTGGG CAGTTTACCG
  721 TAAATACTCC ACCCATTGAC GTCAATGGAA AGTCCCTATT GGCGTTACTA TGGGAACATA
  781 CGTCATTATT GACGTCAATG GGCGGGGGTC GTTGGGCGGT CAGCCAGGCG GGCCATTTAC
  841 CGTAAGTTAT GTAACGACAT GCATCTAATG AGTGAAAGGG CCTCGTACTA CGCCTATTTT
  901 TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA
  961 ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA
 1021 TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AACGCGCGAA TTGCAAGCTC
 1081 TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG
 1141 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC
 1201 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT
 1261 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC
 1321 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA
 1381 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC
 1441 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG
 1501 CGCTTTCTCA ATGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC
 1561 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC
 1621 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA
 1681 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT
 1741 ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG
 1801 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
 1861 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT
 1921 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGC
 1981 CATAACTTCG TATAGCATAC ATTATACGAA GTTATGGCAT GAGATTATCA AAAAGGATCT
 2041 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT
 2101 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC
 2161 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG
 2221 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG
 2281 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT
 2341 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG
 2401 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT
 2461 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA
 2521 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG
 2581 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT
 2641 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA
 2701 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA
 2761 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT
 2821 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT
 2881 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA
 2941 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT
 3001 GAAGCATTTA TCAGGGTTAT TGTCTCATGC CAGGGGTGGG CACACATATT TGATACCAGC
 3061 GATCCCTACA CAGCACATAA TTCAATGCGA CTTCCCTCTA TCGCACATCT TAGACCTTTA
 3121 TTCTCCCTCC AGCACACATC GAAGCTGCCG AGCAAGCCGT TCTCACCAGT CCAAGACCTG
 3181 GCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA
 3241 CATTTCCCCG AAAAGTGCCA CCTGAAATTG TAAACGTTAA TATTTTGTTA AAATTCGCGT
 3301 TAAATTTTTG TTAAATCAGC TCATTTTTTA ACCAATAGGC CGAAATCGGC AAAATCCCTT
 3361 ATAAATCAAA AGAATAGACC GAGATAGGGT TGAGTGTTGT TCCAGTTTGG AACAAGAGTC
 3421 CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA AACCGTCTAT CAGGGCGATG
 3481 GCCCACTACG TGAACCATCA CCCTAATCAA GTTTTTTGGG GTCGAGGTGC CGTAAAGCAC
 3541 TAAATCGGAA CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG CCGGCGAACG
 3601 TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG GCAAGTGTAG
 3661 CGGTCACGCT GCGCGTAACC ACCACACCCG CCGCGCTTAA TGCGCCGCTA CAGGGCGCGT
 3721 CCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC
 3781 TATTACGCCA GCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA
 3841 GGATCGATCC AGACATGATA AGATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC
 3901 AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA
 3961 TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG
 4021 GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT ATGGCTGATT
 4081 ATGATCATGA ACAGACTGTG AGGACTGAGG GGCCTGAAAT GAGCCTTGGG ACTGTGAATC
 4141 TAAAATACAC AAACAATTAG AATCACTAGC TCCTGTGTAT AATATTTTCA TAAATCATAC
 4201 TCAGTAAGCA AAACTCTCAA GCAGCAAGCA TATGCAGCTA GTTTAACACA TTATACACTT
 4261 AAAAATTTTA TATTTACCTT AGAGCTTTAA ATCTCTGTAG GTAGTTTGTC CAATTATGTC
 4321 ACACCACAGA AGTAAGGTTC CTTCACAAAG ATCCCAAGCT AGCAGTTTTC CCAGTCACGA
 4381 CGTTGTAAAA CGACGG
//

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