pFLD

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价格：元

联系方式：I47-825O-882O

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pFLD载体质粒基本信息

出品公司:	Invitrigen
载体名称:	pFLD
质粒类型:	酵母表达载体
高拷贝/低拷贝:	高拷贝
启动子:	FLD1
克隆方法:	多克隆位点，限制性内切酶
载体大小:	4409 bp
5' 测序引物及序列:	--
3' 测序引物及序列:	--
载体标签:	His Tag (6x) ; V5 Epitope Tag
载体抗性:	Ampicillin
筛选标记:	Zeocin
备注:	巴斯德毕赤酵母蛋白表达。诱导剂：甲胺
产品目录号:	V942-20
稳定性:	--
组成型/诱导型:	诱导型
病毒/非病毒:	非病毒

pFLD质粒图谱载体图谱和pFLD载体序列质粒序列多克隆位点信息

pFLD载体图谱

pFLD质粒载体简介

pFLD and pFLDalpha are Pichia pastoris expression vectors designed for methylamine-induced expression and rapid protein purification and detection. The vectors feature:

 FLD promoter for inducible expression with methylamine (1)
 C-terminal V5 epitope and polyhistidine (6xHis) region for convenient detection with an Anti-V5 antibody and purification using nickel-chelating resin
 α-factor secretion signal for efficient transport of proteins to the medium (pFLDalpha only)

pFLD质粒序列载体序列

GCATGCAGGAATCTCTGGCACGGTGCTAATGGTAGTTATCCAACGGAGCTGAGGTAGTCGATATATCTGG
ATATGCCGCCTATAGGATAAAAACAGGAGAGGGTGAACCTTGCTTATGGCTACTAGATTGTTCTTGTACT
CTGAATTCTCATTATGGGAAACTAAACTAATCTCATCTGTGTGTTGCAGTACTATTGAATCGTTGTAGTA
TCTACCTGGAGGGCATTCCATGAATTAGTGAGATAACAGAGTTGGGTAACTAGAGAGAATAATAGACGTA
TGCATGATTACTACACAACGGATGTCGCACTCTTTCCTTAGTTAAAACTATCATCCAATCACAAGATGCG
GGCTGGAAAGACTTGCTCCCGAAGGATAATCTTCTGCTTCTATCTCCCTTCCTCATATGGTTTCGCAGGG
CTCATGCCCCTTCTTCCTTCGAACTGCCCGATGAGGAAGTCCTTAGCCTATCAAAGAATTCGGGACCATC
ATCGATTTTTAGAGCCTTACCTGATCGCAATCAGGATTTCACTACTCATATAAATACATCGCTCAAAGCT
CCAACTTTGCTTGTTCATACAATTCTTGATATTCACACAATTGTTCACGTGGCCCAGCCGGCCGTCTCGG
ATCGGTACCTCGAGCCGCGGCGGCCGCCAGCTTGGGCCCAATTGGGGTGGTAAGCCTATCCCTAACCCTC
TCCTCGGTCTCGATTCTACGGGTGTCGACCATCATCATCATCATCATTGAGTTTGTAGCCTTAGACATGA
CTGTTCCTCAGTTCAAGTTGGGCACTTACGAGAAGACCGGTCTTGCTAGATTCTAATCAAGAGGATGTCA
GAATGCCATTTGCCTGAGAGATGCAGGCTTCATTTTTGATACTTTTTTATTTGTAACCTATATAGTATAG
GATTTTTTTTGTCATTTTGTTTCTTCTCGTACGAGCTTGCTCCTGATCAGCCTATCTCGCAGCTGATGAA
TATCTTGTGGTAGGGGTTTGGGAAAATCATTCGAGTTTGATGTTTTTCTTGGTATTTCCCACTCCTCTTC
AGAGTACAGAAGATTAAGTGAGACCTTCGTTTGTGCGGATCCGGCGCGCCGATCCGCCTGATGCGGTATT
TTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATATGGTGCACTCTCAGTACAATCTGCTCTGAT
GCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCC
CGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATC
ACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATG
GTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAA
TACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAA
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTT
GCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCG
AACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCAC
TTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGC
ATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGA
CAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAAC
GATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGT
TGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAA
CAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGAT
GGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAA
TCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTA
TCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGG
TGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAA
CTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAAC
GTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTT
TCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAA
GAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAG
TGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCT
GTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCG
GATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACA
CCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAG
GTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTAT
CTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGC
GGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCA
CACACCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGC
CGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTCCCCTCTTTCTTCCTCTAGGGTGTCGTTAA
TTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGAGACCGCCTCGTTTCTTTTTCTTCGTCGAAAAAGGCA
ATAAAAATTTTTATCACGTTTCTTTTTCTTGAAAATTTTTTTTTTTGATTTTTTTCTCTTTCGATGACCT
CCCATTGATATTTAAGTTAATAAACGGTCTTCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATT
ACAACTTTTTTTACTTCTTGCTCATTAGAAAGAAAGCATAGCAATCTAATCTAAGGGCGGCGTTGACAAT
TAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCATGGCCAAGTTGA
CCAGTGCCGTTCCGGTGCTCACCGCGCGCGACGTCGCCGGAGCGGTCGAGTTCTGGACCGACCGGCTCGG
GTTCTCCCGGGACTTCGTGGAGGACGACTTCGCCGGTGTGGTCCGGGACGACGTGACCCTGTTCATCAGC
GCGGTCCAGGACCAGGTGGTGCCGGACAACACCCTGGCCTGGGTGTGGGTGCGCGGCCTGGACGAGCTGT
ACGCCGAGTGGTCGGAGGTCGTGTCCACGAACTTCCGGGACGCCTCCGGGCCGGCCATGACCGAGATCGG
CGAGCAGCCGTGGGGGCAGGAGTTCGCCCTGCGCGACCCGGCCGGCAACTGCGTGCACTTCGTGGCCGAG
GAGCAGGACTGACACGTCCGACGCGGCCCGACGGGTCCGAGGCCTCGGAGATCCGTCCCCCTTTTCCTTT
GTCGATATCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAA
AGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAAC
GTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAA
ACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCAAGCTGAATTGCGGCCGCAGATCT

pGEX-6p-1-AT5G48650	pBTB-5
pIZT/V5-His	pYC25
pSP65-F1+	pAAV-TRE-CAG-Fluc
FUW-tetO-hOCT4	pCRE-DD-AmCyan1
pSW510	pFN10A (ACT)
pShuttle-CMV	pIRESpuro3
pCDEF-HA-Ub-K63R	pL-brevisGH1-TA
pAAV-EFS-DIO-GRAB_OT1.0	pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM5A-FLAG
YEp16	pAAVLINKv1-pCALM1-Cre-3'-ITSN1-FLAG
pAAVLINKv1-pCALM1-Cre-3'-RAI1-FLAG	pET28a-mOrange(M163A)
pQE-16	pcDNA3.1+N-DYK-P2A
pcDNA6.2/nTC-Tag-DEST	pAAV-hSyn-Cepheid1s-WPRE
pDEST8	YEp24
pHIL-D2	pBARMTE1
pBABE-HAII	pcDNA3.1-mRFP
pAX01-ComK	pAAVLINK-EF1a-HA-5'-ITSN1
pHY300PLK	pUC19
pHOOK1	pcDNA3.1-CNTRFv1-HA-His
pFR-Luc	pAMβ1
pcDNA4/TO/Myc-His/LacZ	pBluescript II KS(-)
pGL4.18[luc2P/Neo]	pENTR5'/CMVp
pNov.aro-bgl1-640	pUTK21
pCEP4-Flag-N	pIEX-1
pCold-SUMO	pSuper.Retro.Neo
pENTR U6	pMMB66EH
pAAVLINK-EF1a-HA-5-RALGAPB	pFastBac1-His-C
pBad33	pHisB-Sumo-NeMeHg
pLC4-14	pBBR1MCS2-Tac-mCherry
pS-hydro_TA	ppyrG
pQLinkN	pRetroX-Tight-Hyg
pHT01	pEYFP-Mem
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-POGZ-FLAG	pAP1(PMA)-TA-Luc
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PHIP-FLAG	pSwitch
pDM4	pLVX-AmCyan1-N1
pDNAPolbeta_TA	pHR31
pHBT-GFP-NOS	p19D+
pENTR-L1pLaclacZaL4	pAAVLINK-EF1a-HA-5'-DIP2C
pCRII-TOPO/22-1	Pgex-HRV3C
YEp620	pIEx/Bac-3
pDB262	pCEP4-DsRed2
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CADPS-FLAG	p1JI008
pMAL-p5x	pPM408
pGFP22	pGL4.21[luc2P/Puro]
pVK101	pcDNA3.1/Zeo(-)
pGEX-6p-1-hsG3BP2b	pWY113 pET28a_AtmtE2
pAAV-CAG-NES-YTnC	pYES2-EGFP
pIEX-3	pBR327
pET-53-DEST	pLenti6/V5-GW/lacZ
pSEAP2-Basic	pAAVLINK-EF1a-5'-PXDN
pMCS-Green-Renilla-Luc	pRI101-AN

LeGO-iG	pRK5-HA-SH3BP4 W92A
GAG-CRErec	pCI-neo.mCherry-NEDD4
pCutamp	pRK5-HA-Ubiquitin-K29R
pAc-sgRNA-Cas9	pGL3 3'UTR reporter WT 1.3 kb CD274 Hs 3
pXR002:EF1a-dCasRx-2A-EGFP	pcDNA5-T/O-Ds1-mCherry
pEBTet_NAD-Snifit-mito	pLenti-MP2
BCR/ABL P190 transgenic construct	pET16B.Pfu
phage UbiC G3BP1-SNAP	pCDH-DCX-TurboID-V5-puro
pcDNA3.1_AktPH-mCherry	Fuw-dCas9-Dnmt3a_IM
pCRISPR-cBEST	pET28GST-LIC
pAKTaq	pShuttle-CMV-GFP U6promoter
pRH2502	CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGre
pEGFP-C1 F-tractin-EGFP	pDriver
pBiFC-VN155(I152L)	pCAG-FAST-p10
PX551	pTA-HUH
pcDNA3.1-ERBB4	pDawn-sfGFP
pAAVS1-PC-GCaMP6f	pQCXIP-GFP-LacR
LentiGuide_DKO	pLTR-RD114A
pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W	pGHYB
SIRT6 Flag	pEF1a-Usp9x_CD-mCherry
pQUAST>stop>mCD8-GFP	pINDUCER21-puro_Gateway-3xFLAG
pBLO 64.1	pMR-A-3
pJRH-1179 U6-reci Gag-Cas9 v2	pcDNA3.1-TYMS
pT7-SpCas9_sgRNA-site1 (RTW443)	pMIH-TOMM20-Halo
pX330_SpCas9_USP7 Exon 3 gRNA	PB533_15F11-sfGFP
p6XHis-T7(P266L)	pEG302 22aa SunTag NtDRMcd (no NLS) g4+g
EGFP-LC3	pMXs-hSOX2
pHCMV_DUGV_GP	pQCasTns(Vch)-entry(BsaI)
pKO (Hyg)	Myc tag WT PTB
murine TCR OTI-2A.pMIG II	pTM1_VP30_ZEBOV
pCDNA3 Flag p38 alpha	pcDNA3.1 SIRT6_H133Y
mARG1 cassette 3	pCITEp65
PA-GFPmt	pCMT-flp
CAMK2D gRNA (BRDN0001146112)	BB3cK_pGAP_23*_pTEF_Cas9
Double UP mNeon to mScarlet	pCMV-HA-JMJD3
GW1-Peredox-mCherry-NLS	pHIV-Luciferase
AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-	pCI-6.20
pBIG1a	MP6
pLIC-SM-HA	pscAAV-CAG-GFP
pCS2+HyPer7-NLS	pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA
pT7-hMLH1dn for IVT	AdEasier-1 cells (strain)
pBK092	pNG9
GFP-MAP1B	pGP-CMV-Gcamp6m
pLminP_Luc2P_RE4	pcDNA3 Connexin43-GFP-APEX2
pGEX6p-1-BCCP-PupE	pKAR2-Br512
mAzurite-8xbiRhoBAST-24xMS2v6	CD109-bio-His
pBSK	pCMV-mA1-BE3
MG1655 ΔendA ΔrecA (DE3)	pPB-AMBRA1-3xFLAG
pcDNA3.1(+) CircRNA Mini Vector	shYAP1
pSI-Target-AID-NG	pAAV2/9n

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pFLD

买家导航

pFLD载体质粒基本信息

pFLD质粒图谱载体图谱和pFLD载体序列质粒序列多克隆位点信息

pFLD质粒载体简介

pFLD质粒序列载体序列

pFLD载体图谱质粒图谱pdf版和pFLD载体序列质粒序列等相关资料下载

pFLD质粒载体应用举例

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