| 质粒名称: | SunTagng22aa |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 106438 |
| 存储实验室: | Steven Jacobsen |
|
载体骨架基本信息 |
|
| 载体名称: | pMOA34 |
| 完整质粒大小: | 24538 bp |
| 载体类型: | Plant Expression, CRISPR, Synthetic Biology |
| 载体抗性: | 大观霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | SunTagng-22aa/NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS |
| 物种来源: | H. sapiens (human), A. thaliana (mustard weed), Synthetic |
| 插入大小: | 16530bp |
| 3’测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | SunTagng22aa was a gift from Steven Jacobsen (Addgene plasmid # 106438 ; http://n2t.net/addgene:106438 ; RRID:Addgene_106438) |
| 参考文献部分: | Targeted DNA demethylation of the Arabidopsis genome using the human TET1 catalytic domain. Gallego-Bartolome J, Gardiner J, Liu W, Papikian A, Ghoshal B, Kuo HY, Zhao JM, Segal DJ, Jacobsen SE. Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):E2125-E2134. doi: 10.1073/pnas.1716945115. Epub 2018 Feb 14. 10.1073/pnas.1716945115 P |
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载体基本信息
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