pGL4.74[hRluc/TK]

价格:1500元

联系方式:I47-825O-882O

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pGL4.74载体基本信息

出品公司: Promega
载体名称: pGL4.74 ; pGL4.74[hRluc/TK]
质粒类型: 信号通路报告载体;哺乳动物载体;海肾荧光素酶报告载体
高拷贝/低拷贝: --
克隆方法: 限制性内切酶,多克隆位点
启动子: HSV-TK
载体大小: 4237 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: hRluc
载体抗性: 氨苄青霉素
筛选标记:
克隆菌株: TOP10等常规菌株
宿主细胞(系): HEK293等
备注: pGL4.74[hRluc/TK]载体是信号通路报告载体;含海肾荧光素酶报告基因hRluc。
产品目录号: E6921
稳定性: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

pGL4.74载体质粒图谱和多克隆位点信息

pGL4.74载体图谱



pGL4.74 载体特征

pGL4.74载体简介

pGL4.74载体简介
The pGL4.74[hRluc/TK] Vector encodes the luciferase reporter gene hRluc (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been codon optimized for mammalian expression. 
The pGL4.74[hRluc/TK] Vector contains the hRluc luciferase reporter gene and an HSV-TK promoter and can be used as an expression control or a coreporter vector.

pGL4.74载体序列

LOCUS       AY738230                4237 bp    DNA     circular SYN 08-NOV-2004
DEFINITION  Renilla luciferase reporter vector pGL4.74[hRluc/TK], complete
            sequence.
ACCESSION   AY738230
VERSION     AY738230.1  GI:55535642
KEYWORDS    .
SOURCE      Renilla luciferase reporter vector pGL4.74[hRluc/TK]
  ORGANISM  Renilla luciferase reporter vector pGL4.74[hRluc/TK]
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Almond,B., Chauvin,F. and Browning,J.
  TITLE     pGL4 Luciferase Reporter Vectors Technical Manual, TM259, Promega
            Corporation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Almond,B., Chauvin,F. and Browning,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2004) Scientific Communications, Promega
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Renilla luciferase reporter vector
                     pGL4.74[hRluc/TK]"
                     /mol_type="other DNA"
                     /db_xref="taxon:296667"
     regulatory      27..779
                     /regulatory_class="promoter"
                     /note="HSV-TK"
     CDS             815..1750
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase hRluc"
                     /protein_id="AAV52885.1"
                     /db_xref="GI:55535643"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
                     NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
                     AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
                     EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
                     WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
                     EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     misc_feature    1784..2005
                     /note="SV40 late poly(A) region"
     primer_bind     2071..2090
                     /note="reporter vector primer 4 (RVPrimer4)"
     rep_origin      2330
                     /note="ColE1-derived"
     gene            complement(3119..3979)
                     /gene="ampR"
     CDS             complement(3119..3979)
                     /gene="ampR"
                     /note="AmpR"
                     /codon_start=1
                     /transl_table=11
                     /product="synthetic beta-lactamase"
                     /protein_id="AAV52886.1"
                     /db_xref="GI:55535644"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     regulatory      4084..4237
                     /regulatory_class="polyA_signal_sequence"
                     /note="synthetic; transcriptional pause site"
     primer_bind     complement(4186..4205)
                     /note="reporter vector primer 3 (RVPrimer3)"
ORIGIN      
        1 ggcctaactg gccggtacct gagtctaaat gagtcttcgg acctcgcggg ggccgcttaa
       61 gcggtggtta gggtttgtct gacgcggggg gagggggaag gaacgaaaca ctctcattcg
      121 gaggcggctc ggggtttggt cttggtggcc acgggcacgc agaagagcgc cgcgatcctc
      181 ttaagcaccc ccccgccctc cgtggaggcg ggggtttggt cggcgggtgg taactggcgg
      241 gccgctgact cgggcgggtc gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac
      301 ccccgggcgg cgccgccgcg gcggcgacgg gctcgctggg tcctaggctc catggggacc
      361 gtatacgtgg acaggctctg gagcatccgc acgactgcgg tgatattacc ggagaccttc
      421 tgcgggacga gccgggtcac gcggctgacg cggagcgtcc gttgggcgac aaacaccagg
      481 acggggcaca ggtacactat cttgtcaccc ggaggcgcga gggactgcag gagcttcagg
      541 gagtggcgca gctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg
      601 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg
      661 tcattggcga attcgaacac gcagatgcag tcggggcggc gcggtcccag gtccacttcg
      721 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaaa
      781 agcttggcaa tccggtactg ttggtaaagc caccatggct tccaaggtgt acgaccccga
      841 gcaacgcaaa cgcatgatca ctgggcctca gtggtgggct cgctgcaagc aaatgaacgt
      901 gctggactcc ttcatcaact actatgattc cgagaagcac gccgagaacg ccgtgatttt
      961 tctgcatggt aacgctgcct ccagctacct gtggaggcac gtcgtgcctc acatcgagcc
     1021 cgtggctaga tgcatcatcc ctgatctgat cggaatgggt aagtccggca agagcgggaa
     1081 tggctcatat cgcctcctgg atcactacaa gtacctcacc gcttggttcg agctgctgaa
     1141 ccttccaaag aaaatcatct ttgtgggcca cgactggggg gcttgtctgg cctttcacta
     1201 ctcctacgag caccaagaca agatcaaggc catcgtccat gctgagagtg tcgtggacgt
     1261 gatcgagtcc tgggacgagt ggcctgacat cgaggaggat atcgccctga tcaagagcga
     1321 agagggcgag aaaatggtgc ttgagaataa cttcttcgtc gagaccatgc tcccaagcaa
     1381 gatcatgcgg aaactggagc ctgaggagtt cgctgcctac ctggagccat tcaaggagaa
     1441 gggcgaggtt agacggccta ccctctcctg gcctcgcgag atccctctcg ttaagggagg
     1501 caagcccgac gtcgtccaga ttgtccgcaa ctacaacgcc taccttcggg ccagcgacga
     1561 tctgcctaag atgttcatcg agtccgaccc tgggttcttt tccaacgcta ttgtcgaggg
     1621 agctaagaag ttccctaaca ccgagttcgt gaaggtgaag ggcctccact tcagccagga
     1681 ggacgctcca gatgaaatgg gtaagtacat caagagcttc gtggagcgcg tgctgaagaa
     1741 cgagcagtaa ttctagagtc ggggcggccg gccgcttcga gcagacatga taagatacat
     1801 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     1861 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     1921 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     1981 gtaaaacctc tacaaatgtg gtaaaatcga taaggatccg tcgaccgatg cccttgagag
     2041 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
     2101 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc
     2161 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     2221 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     2281 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2341 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     2401 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2461 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     2521 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     2581 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     2641 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     2701 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     2761 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     2821 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     2881 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     2941 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     3001 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     3061 agtatatatg agtaaacttg gtctgacagc ggccgcaaat gctaaaccac tgcagtggtt
     3121 accagtgctt gatcagtgag gcaccgatct cagcgatctg cctatttcgt tcgtccatag
     3181 tggcctgact ccccgtcgtg tagatcacta cgattcgtga gggcttacca tcaggcccca
     3241 gcgcagcaat gatgccgcga gagccgcgtt caccggcccc cgatttgtca gcaatgaacc
     3301 agccagcagg gagggccgag cgaagaagtg gtcctgctac tttgtccgcc tccatccagt
     3361 ctatgagctg ctgtcgtgat gctagagtaa gaagttcgcc agtgagtagt ttccgaagag
     3421 ttgtggccat tgctactggc atcgtggtat cacgctcgtc gttcggtatg gcttcgttca
     3481 actctggttc ccagcggtca agccgggtca catgatcacc catattatga agaaatgcag
     3541 tcagctcctt agggcctccg atcgttgtca gaagtaagtt ggccgcggtg ttgtcgctca
     3601 tggtaatggc agcactacac aattctctta ccgtcatgcc atccgtaaga tgcttttccg
     3661 tgaccggcga gtactcaacc aagtcgtttt gtgagtagtg tatacggcga ccaagctgct
     3721 cttgcccggc gtctatacgg gacaacaccg cgccacatag cagtactttg aaagtgctca
     3781 tcatcgggaa tcgttcttcg gggcggaaag actcaaggat cttgccgcta ttgagatcca
     3841 gttcgatata gcccactctt gcacccagtt gatcttcagc atcttttact ttcaccagcg
     3901 tttcggggtg tgcaaaaaca ggcaagcaaa atgccgcaaa gaagggaatg agtgcgacac
     3961 gaaaatgttg gatgctcata ctcgtccttt ttcaatatta ttgaagcatt tatcagggtt
     4021 actagtacgt ctctcaagga taagtaagta atattaaggt acgggaggta ttggacaggc
     4081 cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg tgaatcgata
     4141 gtactaacat acgctctcca tcaaaacaaa acgaaacaaa acaaactagc aaaataggct
     4201 gtccccagtg caagtgcagg tgccagaaca tttctct
//

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