买家导航
pDream2.1/MCS载体 pDream2.1MCS质粒基本信息
pDream2.1/MCS质粒图谱 pDream2.1MCS载体图谱和pDream2.1/MCS载体序列 pDream2.1MCS质粒序列多克隆位点信息
pDream2.1/MCS质粒 pDream2.1MCS载体简介
pDream2.1/MCS质粒序列 pDream2.1MCS载体序列
LOCUS pDream2.1 MCS 7159 bp ds-DNA circular SYN 23-FEB-2013
DEFINITION Vector for expression of N-terminally FLAG-tagged proteins in
bacterial, insect, and mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pDream2.1 MCS
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7159)
AUTHORS GenScript
TITLE Direct Submission
JOURNAL Exported Saturday, January 11, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7159
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_recomb 181..1008
/note="baculovirus recombination region (lef2/ORF603)"
/note="contains ORF603 and part of lef2"
CDS 181..305
/codon_start=3
/product="baculovirus late expression factor 2"
/note="lef2"
/translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
CDS complement(343..948)
/codon_start=1
/product="baculovirus ORF603 protein"
/note="ORF603"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
enhancer 1086..1389
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 1390..1593
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 1615..1633
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 1634..1658
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter 1661..1770
/note="p10 promoter"
/note="baculovirus promoter for expression in insect cells"
protein_bind 1772..1796
/gene="mutant version of attB"
/bound_moiety="BP Clonase(TM)"
/note="attB1"
/note="recombination site for the Gateway(R) BP reaction"
CDS 1810..1812
/codon_start=1
/product="start codon"
/note="ATG"
/translation="M"
CDS 1813..1836
/codon_start=1
/product="FLAG epitope tag, followed by an enterokinase
cleavage site"
/note="FLAG"
/translation="DYKDDDDK"
misc_feature 1837..1882
/note="MCS"
/note="multiple cloning site"
protein_bind 1905..1929
/gene="mutant version of attB"
/bound_moiety="BP clonase(TM)"
/note="attB2"
/note="recombination site for the Gateway(R) BP reaction"
polyA_signal 1931..2407
/note="hGH poly(A) signal"
/note="human growth hormone polyadenylation signal"
terminator 2408..2455
/note="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
rep_origin 2548..2976
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2990..3319
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 3170..3305
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 3386..4180
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 4354..4475
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
polyA_site 4519..4574
/note="beta-globin poly(A) signal"
/note="rabbit beta-globin polyadenylation signal"
misc_recomb 4722..5427
/note="baculovirus recombination region (ORF1629)"
/note="contains part of ORF1629"
CDS complement(4839..5427)
/codon_start=2
/product="baculovirus capsid-associated protein"
/note="ORF1629"
/note="required for viral replication"
/translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
rep_origin complement(5637..6224)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6237..7097)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
ORIGIN
1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
1801 agatatacca tggattacaa ggatgacgac gataagggat ccgagatctg gaattcggta
1861 ccgagctcaa gcttgcggcc gcctatagtg tcacctaaat cgtaacccag ctttcttgta
1921 caaagtggtc gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt
1981 tgccactcca gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga
2041 ctaggtgtcc ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt
2101 tgggatgaca acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc
2161 acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc
2221 tcccgagttg ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg
2281 gtagagacgg ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat
2341 ctacccacct tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc
2401 tgtccttcta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgtctag
2461 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
2521 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
2581 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
2641 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
2701 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
2761 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
2821 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
2881 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
2941 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
3001 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
3061 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
3121 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
3181 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
3241 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
3301 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
3361 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
3421 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
3481 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
3541 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
3601 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
3661 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
3721 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
3781 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
3841 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
3901 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
3961 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
4021 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
4081 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
4141 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
4201 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
4261 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
4321 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
4381 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
4441 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgttcatg aagccccttg
4501 agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt
4561 ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg
4621 agtatttggt ttagagtttg gcaacatatg cccatatgta actagcataa ccccttgggg
4681 cctctaaacg ggtcttgagg ggttttttgc tgaaagcatg cggaggaaat tctccttgaa
4741 gtttccctgg tgttcaaagt aaaggagttt gcaccagacg cacctctgtt cactggtccg
4801 gcgtattaaa acacgataca ttgttattag tacatttatt aagcgctaga ttctgtgcgt
4861 tgttgattta cagacaattg ttgtacgtat tttaataatt cattaaattt ataatcttta
4921 gggtggtatg ttagagcgaa aatcaaatga ttttcagcgt ctttatatct gaatttaaat
4981 attaaatcct caatagattt gtaaaatagg tttcgattag tttcaaacaa gggttgtttt
5041 tccgaaccga tggctggact atctaatgga ttttcgctca acgccacaaa acttgccaaa
5101 tcttgtagca gcaatctagc tttgtcgata ttcgtttgtg ttttgttttg taataaaggt
5161 tcgacgtcgt tcaaaatatt atgcgctttt gtatttcttt catcactgtc gttagtgtac
5221 aattgactcg acgtaaacac gttaaataga gcttggacat atttaacatc gggcgtgtta
5281 gctttattag gccgattatc gtcgtcgtcc caaccctcgt cgttagaagt tgcttccgaa
5341 gacgattttg ccatagccac acgacgccta ttaattgtgt cggctaacac gtccgcgatc
5401 aaatttgtag ttgagctttt tggaattgcg atcgcataac ttcgtatagc atacattata
5461 cgaagttata agctcggaac gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
5521 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
5581 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
5641 cataggctcc gccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
5701 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
5761 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
5821 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
5881 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
5941 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
6001 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
6061 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
6121 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
6181 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgttac
6241 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
6301 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
6361 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
6421 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
6481 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
6541 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
6601 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
6661 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
6721 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
6781 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
6841 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
6901 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
6961 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
7021 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
7081 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
7141 tgtctcatgt ccgcgcgtt
//
