pENTR/H1/TO

价格:2500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Invitrogen
载体名称: pENTR/H1/TO
质粒类型: Gateway载体;入门载体
高拷贝/低拷贝: 高拷贝
启动子:
克隆方法: 限制性内切酶;多克隆位点
载体大小: 3869 bp
5' 测序引物及序列: pENTR-F: CTACAAACTCTTCCTGTTAGTTAG
3' 测序引物及序列: pENTR-R: ATGGCTCATAACACCCCTTG
载体标签:
载体抗性: 卡那霉素
真核筛选标记:
克隆菌株: DH5aTOP10感受态细胞
产品目录号: --
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pENTR/H1/TO载体图谱



pENTR/H1/TO多克隆位点

载体简介

载体序列

LOCUS       pENTR/H1/TO    3869 bp    DNA    SYN    06-Mar-2014
DEFINITION  pENTR/H1/TO
ACCESSION    
KEYWORDS    
SOURCE    
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="pENTR/H1/TO"
                     /mol_type="other DNA"
     terminator      complement(268..295)
                     /label="rrnB_T2_terminator"
     terminator      complement(427..470)
                     /label="rrnB_T1_terminator"
     misc_feature    complement(532..551)
                     /label="EBV_rev_primer"
     terminator      complement(518..637)
                     /label="SV40_PA_terminator"
     misc_feature    complement(772..1146)
                     /label="sh_ble"
     gene            complement(775..1146)
                     /label="bleo"
                     /gene="bleo"
     promoter        complement(1147..1214)
                     /label="EM7_promoter"
     misc_feature    complement(1328..1347)
                     /label="SV40pro_F_primer"
     rep_origin      complement(1332..1409)
                     /label="SV40_origin"
     promoter        complement(1308..1576)
                     /label="SV40_promoter"
     misc_feature    1379..1594
                     /label="SV40_enhancer"
     misc_feature    1574..1594
                     /label="pBABE_3_primer"
     misc_feature    1633..1655
                     /label="M13_pUC_fwd_primer"
     misc_feature    1648..1664
                     /label="M13_forward20_primer"
     misc_feature    complement(1680..1779)
                     /label="attL1"
     misc_feature    1848..1867
                     /label="H1_primer"
     misc_feature    complement(1978..1994)
                     /label="pBluescriptSK_primer"
     misc_feature    1994..2093
                     /label="attL2"
     promoter        complement(2111..2129)
                     /label="T7_promoter"
     misc_feature    complement(2130..2148)
                     /label="M13_reverse_primer"
     CDS             2263..3072
                     /label="KanR2"
                     /gene="KanR2"
                     /note="ORF frame 1"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGK
                     PDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD
                     ASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRV
                     GIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
     gene            2263..3072
                     /label="KanR2"
                     /gene="KanR2"
     rep_origin      3203..3822
                     /label="pBR322_origin"
ORIGIN
    1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 
   61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 
  121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 
  181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 
  241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 
  301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 
  361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 
  421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 
  481 GCAGTTCCCT ACTCTCGCGT TAATTCGCCC TTAGACATGA TAAGATACAT TGATGAGTTT 
  541 GGACAAACCA CAACTAGAAT GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT 
  601 ATTGCTTTAT TTGTAACCAT TATAAGCTGC AATAAACAAG TTGGGGTGGG CGAAGAACTC 
  661 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
  721 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTAGCACGT GTCAGTCCTG 
  781 CTCCTCGGCC ACGAAGTGCA CGCAGTTGCC GGCCGGGTCG CGCAGGGCGA ACTCCCGCCC 
  841 CCACGGCTGC TCGCCGATCT CGGTCATGGC CGGCCCGGAG GCGTCCCGGA AGTTCGTGGA 
  901 CACGACCTCC GACCACTCGG CGTACAGCTC GTCCAGGCCG CGCACCCACA CCCAGGCCAG 
  961 GGTGTTGTCC GGCACCATCT GGTCCTGGAC CGCGCTGATG AACAGGGTCA CGTCGTCCCG 
 1021 GACCACACCG GCGAAGTCGT CCTCCACGAA GTCCCGGGAG AACCCGAGCC GGTCGGTCCA 
 1081 GAACTCGACC GCTCCGGCGA CGTCGCGCGC GGTGAGCACC GGAACGGCAC TGGTCAACTT 
 1141 GGCCATGGTT TAGTTCCTCA CCTTGTCGTA TTATACTATG CCGATATACT ATGCCGATGA 
 1201 TTAATTGTCA ACACGTGCTG ATCAGATCCG AAAATGGATA TACAAGCTCC CGGGAGCTTT 
 1261 TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCA 
 1321 GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG 
 1381 GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT 
 1441 GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA 
 1501 CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT 
 1561 GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCC GAAGGGCGAA TTAACGCTAG 
 1621 CATGGATGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTCTTAAG CTCGGGCCCC 
 1681 AAATAATGAT TTTATTTTGA CTGATAGTGA CCTGTTCGTT GCAACAAATT GATGAGCAAT 
 1741 GCTTTTTTAT AATGCCAACT TTGTACAAAA AAGCAGGCTT TAAAGGAACC AATTCAGTCG 
 1801 ACTGGATCCG GTACCGGGCC CCCCCTCGAA GATCTAATAT TTGCATGTCG CTATGTGTTC 
 1861 TGGGAAATCA CCATAAACGT GAAATCCCTA TCAGTGATAG AGACTTATAA GTTCCCTATC 
 1921 AGTGATAGAG ACACCTTTTT TGTCGAGCTT CCTTCGGGAA GCTCTCCATA TTTTTTGGAT 
 1981 CCACTAGTTC TAGACCCAGC TTTCTTGTAC AAAGTTGGCA TTATAAGAAA GCATTGCTTA 
 2041 TCAATTTGTT GCAACGAACA GGTCACTATC AGTCAAAATA AAATCATTAT TTGCCATCCA 
 2101 GCTGATATCC CCTATAGTGA GTCGTATTAC ATGGTCATAG CTGTTTCCTG GCAGCTCTGG 
 2161 CCCGTGTCTC AAAATCTCTG ATGTTACATT GCACAAGATA AAAATATATC ATCATGAACA 
 2221 ATAAAACTGT CTGCTTACAT AAACAGTAAT ACAAGGGGTG TTATGAGCCA TATTCAACGG 
 2281 GAAACGTCGA GGCCGCGATT AAATTCCAAC ATGGATGCTG ATTTATATGG GTATAAATGG 
 2341 GCTCGCGATA ATGTCGGGCA ATCAGGTGCG ACAATCTATC GCTTGTATGG GAAGCCCGAT 
 2401 GCGCCAGAGT TGTTTCTGAA ACATGGCAAA GGTAGCGTTG CCAATGATGT TACAGATGAG 
 2461 ATGGTCAGAC TAAACTGGCT GACGGAATTT ATGCCTCTTC CGACCATCAA GCATTTTATC 
 2521 CGTACTCCTG ATGATGCATG GTTACTCACC ACTGCGATCC CCGGAAAAAC AGCATTCCAG 
 2581 GTATTAGAAG AATATCCTGA TTCAGGTGAA AATATTGTTG ATGCGCTGGC AGTGTTCCTG 
 2641 CGCCGGTTGC ATTCGATTCC TGTTTGTAAT TGTCCTTTTA ACAGCGATCG CGTATTTCGT 
 2701 CTCGCTCAGG CGCAATCACG AATGAATAAC GGTTTGGTTG ATGCGAGTGA TTTTGATGAC 
 2761 GAGCGTAATG GCTGGCCTGT TGAACAAGTC TGGAAAGAAA TGCATAAACT TTTGCCATTC 
 2821 TCACCGGATT CAGTCGTCAC TCATGGTGAT TTCTCACTTG ATAACCTTAT TTTTGACGAG 
 2881 GGGAAATTAA TAGGTTGTAT TGATGTTGGA CGAGTCGGAA TCGCAGACCG ATACCAGGAT 
 2941 CTTGCCATCC TATGGAACTG CCTCGGTGAG TTTTCTCCTT CATTACAGAA ACGGCTTTTT 
 3001 CAAAAATATG GTATTGATAA TCCTGATATG AATAAATTGC AGTTTCATTT GATGCTCGAT 
 3061 GAGTTTTTCT AATCAGAATT GGTTAATTGG TTGTAACACT GGCAGAGCAT TACGCTGACT 
 3121 TGACGGGACG GCGCAAGCTC ATGACCAAAA TCCCTTAACG TGAGTTACGC GTCGTTCCAC 
 3181 TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC 
 3241 GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT 
 3301 CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT 
 3361 ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT 
 3421 ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT 
 3481 CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG 
 3541 GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA 
 3601 CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG 
 3661 GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG 
 3721 TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC 
 3781 TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG 
 3841 GCCTTTTGCT GGCCTTTTGC TCACATGTT
//

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