| 质粒名称: | pEF6-CSPG4-myc-his |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 69037 |
| 存储实验室: | Wensheng Wei |
|
载体骨架基本信息 |
|
| 载体名称: | pEF6-Myc-His-B |
| 载体出品公司: | Life Technologies |
| 空载体大小: | 5802 bp |
| 完整质粒大小: | 12732 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | Blasticidin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | CSPG4、MCSP; NG2; HMW-MAA |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 6966 bp |
| 基因突变: | none |
| GenBank编号 | 1464 |
| 启动子: | EF-1 alpha |
| 标签/融合蛋白: |
Myc (C terminal on insert)
His (C terminal on insert)
|
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | Spe I (not destroyed) |
| 3’克隆位点: | Not I (not destroyed) |
| 5’测序引物 | T7 |
| 3’测序引物 | BGH Reverse |
|
文献引用方法 |
|
| 材料和方法部分: | pEF6-CSPG4-myc-his was a gift from Wensheng Wei (Addgene plasmid # 69037 ; http://n2t.net/addgene:69037 ; RRID:Addgene_69037) |
| 参考文献部分: | Chondroitin sulfate proteoglycan 4 functions as the cellular receptor for Clostridium difficile toxin B. Yuan P, Zhang H, Cai C, Zhu S, Zhou Y, Yang X, He R, Li C, Guo S, Li S, Huang T, Perez-Cordon G, Feng H, Wei W. Cell Res. 2015 Feb;25(2):157-68. doi: 10.1038/cr.2014.169. Epub 2014 Dec 30. 10.1038/cr.2014.169 PubMed 25547119 |
买家导航
pEF6-CSPG4-myc-his载体质粒基本信息
pEF6-CSPG4-myc-his质粒图谱载体图谱和pEF6-CSPG4-myc-his载体序列质粒序列多克隆位点信息
pEF6-CSPG4-myc-his质粒载体简介
pEF6-CSPG4-myc-his质粒序列载体序列
LOCUS pEF6-CSPG4-myc-his 12732 bp DNA circular SYN 14-APR-2025
DEFINITION Expression of CSPG4 in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12732)
AUTHORS Yuan P, Zhang H, Cai C, Zhu S, Zhou Y, Yang X, He R, Li C, Guo S, Li
S, Huang T, Perez-Cordon G, Feng H, Wei W
TITLE Chondroitin sulfate proteoglycan 4 functions as the cellular
receptor for Clostridium difficile toxin B.
JOURNAL Cell Res. 2015 Feb;25(2):157-68. doi: 10.1038/cr.2014.169. Epub 2014
Dec 30.
PUBMED 25547119
REFERENCE 2 (bases 1 to 12732)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1038/cr.2014.169"; journalName: "Cell Res"; date: "2015-02";
volume: "25"; issue: "2"; pages: "157-68"
COMMENT Sequence Label: pEF6-CSPG4-myc-his
FEATURES Location/Qualifiers
source 1..12732
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
promoter 351..1529
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 582..1520
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 1477..1497
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
primer_bind 1546..1565
/label=T7
/note="T7 promoter, forward primer"
promoter 1546..1564
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 8614..8643
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 8659..8676
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(8699..8716)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 8705..8929
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 8975..9403
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(9062..9081)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 9272..9293
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(9412..9432)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 9417..9746
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 9597..9732
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 9659..9678
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 9794..9841
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 9860..10258
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/label=BSD
/note="confers resistance to blasticidin"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
polyA_signal 10416..10537
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(10453..10472)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 10507..10526
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(10586..10602)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(10586..10602)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(10599..10621)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 10610..10626
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(10634..10640,10641..10658,10659..10664))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 10679..10700
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(10817..10834)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(10977..11565)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(11057..11076)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(11736..12527,12528..12596))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 12359..12378
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(12597..12701)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga
301 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg
361 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
421 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
481 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
541 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt
601 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct
661 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
721 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct
781 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
841 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
901 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
961 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
1021 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
1081 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
1141 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
1201 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
1261 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
1321 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
1381 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
1441 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
1501 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta
1561 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac tagtatgcag
1621 tccgggccgc ggcccccact tccagccccc ggcctggcct tggctttgac cctgactatg
1681 ttggccagac ttgcatccgc ggcttccttc ttcggtgaga accacctgga ggtgcctgtg
1741 gccacggctc tgaccgacat agacctgcag ctgcagttct ccacgtccca gcccgaagcc
1801 ctccttctcc tggcagcagg cccagctgac cacctcctgc tgcagctcta ctctggacgc
1861 ctgcaggtca gacttgttct gggccaggag gagctgaggc tgcagactcc agcagagacg
1921 ctgctgagtg actccatccc ccacactgtg gtgctgactg tcgtagaggg ctgggccacg
1981 ttgtcagtcg atgggtttct gaacgcctcc tcagcagtcc caggagcccc cctagaggtc
2041 ccctatgggc tctttgttgg gggcactggg acccttggcc tgccctacct gaggggaacc
2101 agccgacccc tgaggggttg cctccatgca gccaccctca atggccgcag cctcctccgg
2161 cctctgaccc ccgatgtgca tgagggctgt gctgaagagt tttctgccag tgatgatgtg
2221 gccctgggct tctctgggcc ccactctctg gctgccttcc ctgcctgggg cactcaggac
2281 gaaggaaccc tagagtttac actcaccaca cagagccggc aggcaccctt ggccttccag
2341 gcagggggcc ggcgtgggga cttcatctat gtggacatat ttgagggcca cctgcgggcc
2401 gtggtggaga agggccaggg taccgtattg ctccacaaca gcgtgcctgt ggccgatggg
2461 cagccccatg aggtcagtgt ccacatcaat gctcaccggc tggaaatctc cgtggaccag
2521 taccctacgc atacttcgaa ccgaggagtc ctcagctacc tggagccacg gggcagtctc
2581 cttctcgggg ggctggatgc agaggcctct cgtcacctcc aggaacaccg cctgggcctg
2641 acaccagagg ccaccaatgc ctccctgctg ggctgcatgg aagacctcag tgtcaatggc
2701 cagaggcggg ggctgcggga agctttgctg acgcgcaaca tggcagccgg ctgcaggctg
2761 gaggaggagg agtatgagga cgatgcctat ggacattatg aagctttctc caccctggcc
2821 cctgaggctt ggccggccat ggagctgcct gagccatgcg tgcctgagcc agggctgcct
2881 cctgtctttg ccaatttcac ccagctgctg actatcagcc cactggtggt ggccgagggg
2941 ggcacagcct ggcttgagtg gaggcatgtg cagcccacgc tggacctgat ggaggctgag
3001 ctgcgcaaat cccaggtgct gttcagcgtg acccgagggg cacgccatgg cgagctcgag
3061 ctggacatcc cgggagccca ggcacgaaaa atgttcaccc tcctggacgt ggtgaaccgc
3121 aaggcccgct tcatccacga tggctctgag gacacctccg accagctggt gctggaggtg
3181 tcggtgacgg ctcgggtgcc catgccctca tgccttcgga ggggccaaac atacctcctg
3241 cccatccagg tcaaccctgt caatgaccca ccccacatca tcttcccaca tggcagcctc
3301 atggtgatcc tggaacacac gcagaagccg ctggggcctg aggttttcca ggcctatgac
3361 ccggactctg cctgtgaggg cctcaccttc caggtccttg gcacctcctc tggcctcccc
3421 gtggagcgcc gagaccagcc tggggagccg gcgaccgagt tctcctgccg ggagttggag
3481 gccggcagcc tagtctatgt ccaccgcggt ggtcctgcac aggacttgac gttccgggtc
3541 agcgatggac tgcaggccag ccccccggcc acgctgaagg tggtggccat ccggccggcc
3601 atacagatcc accgcagcac agggttgcga ctggcccaag gctctgccat gcccatcttg
3661 cccgccaacc tgtcggtgga gaccaatgcc gtggggcagg atgtgagcgt gctgttccgc
3721 gtcactgggg ccctgcagtt tggggagctg cagaagcagg gggcaggtgg ggtggagggt
3781 gctgagtggt gggccacaca ggcgttccac cagcgggatg tggagcaggg ccgcgtgagg
3841 tacctgagca ctgacccaca gcaccacgct tacgacaccg tggagaacct ggccctggag
3901 gtgcaggtgg gccaggagat cctgagcaat ctgtccttcc cagtgaccat ccagagagcc
3961 actgtgtgga tgctgcggct ggagccactg cacactcaga acacccagca ggagaccctc
4021 accacagccc acctggaggc caccctggag gaggcaggcc caagcccccc aaccttccat
4081 tatgaggtgg ttcaggctcc caggaaaggc aaccttcaac tacagggcac aaggctgtca
4141 gatggccagg gcttcaccca ggatgacata caggctggcc gggtgaccta tggggccaca
4201 gcacgtgcct cagaggcagt cgaggacacc ttccgtttcc gtgtcacagc tccaccatat
4261 ttctccccac tctatacctt ccccatccac attggtggtg acccagatgc gcctgtcctc
4321 accaatgtcc tcctcgtggt gcctgagggt ggtgagggtg tcctctctgc tgaccacctc
4381 tttgtcaaga gtctcaacag tgccagctac ctctatgagg tcatggagcg gccccgccat
4441 gggaggttgg cttggcgtgg gacacaggac aagaccacta tggtgacatc cttcaccaat
4501 gaagacctgt tgcgtggccg gctggtctac cagcatgatg actccgagac cacagaagat
4561 gatatcccat ttgttgctac ccgccagggc gagagcagtg gtgacatggc ctgggaggag
4621 gtacggggtg tcttccgagt ggccatccag cccgtgaatg accacgcccc tgtgcagacc
4681 atcagccgga tcttccatgt ggcccggggt gggcggcggc tgctgactac agacgacgtg
4741 gccttcagcg atgctgactc gggctttgct gacgcccagc tggtgcttac ccgcaaggac
4801 ctcctctttg gcagtatcgt ggccgtagat gagcccacgc ggcccatcta ccgcttcacc
4861 caggaggacc tcaggaagag gcgagtactg ttcgtgcact caggggctga ccgtggctgg
4921 atccagctgc aggtgtccga cgggcaacac caggccactg cgctgctgga ggtgcaggcc
4981 tcggaaccct acctccgtgt ggccaacggc tccagccttg tggtccctca aggaggccag
5041 ggcaccatcg acacggccgt gctccacctg gacaccaacc tcgacatccg cagtggggat
5101 gaggtccact accacgtcac agctggccct cgctggggac agctagtccg ggctggtcag
5161 ccagccacag ccttctccca gcaggacctg ctggatgggg ccgttctcta tagccacaat
5221 ggcagcctca gcccccgcga caccatggcc ttctccgtgg aagcagggcc agtgcacacg
5281 gatgccaccc tacaagtgac cattgcccta gagggcccac tggccccact gaagctggtc
5341 cggcacaaga agatctacgt cttccaggga gaggcagctg agatcagaag ggaccagctg
5401 gaggcagccc aggaggcagt gccacctgca gacatcgtat tctcagtgaa gagcccaccg
5461 agtgccggct acctggtgat ggtgtcgcgt ggcgccttgg cagatgagcc acccagcctg
5521 gaccccgtgc agagcttctc ccaggaggca gtggacacag gcagggtcct gtacctgcac
5581 tcccgccctg aggcctggag cgatgccttc tcgctggatg tggcctcagg cctgggtgct
5641 cccctcgagg gcgtccttgt ggagctggag gtgctgcccg ctgccatccc actagaggcg
5701 caaaacttca gcgtccctga gggtggcagc ctcaccctgg cccctccact gctccgtgtc
5761 tccgggccct acttccccac tctcctgggc ctcagcctgc aggtgctgga gccaccccag
5821 catggagccc tgcagaagga ggacggacct caagccagga ccctcagcgc cttctcctgg
5881 agaatggtgg aagagcagct gatccgctac gtgcatgacg ggagcgagac actgacagac
5941 agttttgtcc tgatggctaa tgcctccgag atggatcgcc agagccatcc tgtggccttc
6001 actgtcactg tcctgcctgt caatgaccaa ccccccatcc tcactacaaa cacaggcctg
6061 cagatgtggg agggggccac tgcgcccatc cctgcggagg ctctgaggag cacggacggc
6121 gactctgggt ctgaggatct ggtctacacc atcgagcagc ccagcaacgg gcgggtagtg
6181 ctgcgggggg cgccgggcac tgaggtgcgc agcttcacgc aggcccagct ggacggcggg
6241 ctcgtgctgt tctcacacag aggaaccctg gatggaggct tccgcttccg cctctctgac
6301 ggcgagcaca cttcccccgg acacttcttc cgagtgacgg cccagaagca agtgctcctc
6361 tcgctgaagg gcagccagac actgactgtc tgcccagggt ccgtccagcc actcagcagt
6421 cagaccctca gggccagctc cagcgcaggc actgaccccc agctcctgct ctaccgtgtg
6481 gtgcggggcc cccagctagg ccggctgttc cacgcccagc aggacagcac aggggaggcc
6541 ctggtgaact tcactcaggc agaggtctac gctgggaata ttctgtatga gcatgagatg
6601 ccccccgagc ccttttggga ggcccatgat accctagagc tccagctgtc ctcgccgcct
6661 gcccgggacg tggccgccac ccttgctgtg gctgtgtctt ttgaggctgc ctgtccccag
6721 cgccccagcc acctctggaa gaacaaaggt ctctgggtcc ccgagggcca gcgggccagg
6781 atcaccgtgg ctgctctgga tgcctccaat ctcttggcca gcgttccatc accccagcgc
6841 tcagagcatg atgtgctctt ccaggtcaca cagttcccca gccggggcca gctgttggtg
6901 tccgaggagc ccctccatgc tgggcagccc cacttcctgc agtcccagct ggctgcaggg
6961 cagctagtgt atgcccacgg cggtgggggc acccagcagg atggcttcca ctttcgtgcc
7021 cacctccagg ggccagcagg ggcctccgtg gctggacccc aaacctcaga ggcctttgcc
7081 atcacggtga gggatgtaaa tgagcggccc cctcagccac aggcctctgt cccgctccgg
7141 ctcacccgag gctctcgtgc ccccatctcc cgggcccagc tgagtgtggt ggacccagac
7201 tcagctcctg gggagattga gtacgaggtc cagcgggcac cccacaacgg cttcctcagc
7261 ctggtgggtg gtggcctggg gcccgtgacc cgcttcacgc aagccgatgt ggattcaggg
7321 cggctggcct tcgtggccaa cgggagcagc gtggcaggca tcttccagct gagcatgtct
7381 gatggggcca gcccacccct gcccatgtcc ctggctgtgg acatcctacc atccgccatc
7441 gaggtgcagc tgcgggcacc cctggaggtg ccccaagctt tggggcgctc ctcactgagc
7501 cagcagcagc tccgggtggt ttcagatcgg gaggagccag aggcagcata ccgcctcatc
7561 cagggacccc agtatgggca tctcctggtg ggcgggcggc ccacctcggc cttcagccaa
7621 ttccagatag accagggcga ggtggtcttt gccttcacca acttctcctc ctctcatgac
7681 cacttcagag tcctggcact ggctaggggt gtcaatgcat cagccgtagt gaacgtcact
7741 gtgagggctc tgctgcatgt gtgggcaggt gggccatggc cccagggtgc caccctgcgc
7801 ctggacccca ccgtcctaga tgctggcgag ctggccaacc gcacaggcag tgtgccgcgc
7861 ttccgcctcc tggagggacc ccggcatggc cgcgtggtcc gcgtgccccg agccaggacg
7921 gagcccgggg gcagccagct ggtggagcag ttcactcagc aggaccttga ggacgggagg
7981 ctggggctgg aggtgggcag gccagagggg agggcccccg gccccgcagg tgacagtctc
8041 actctggagc tgtgggcaca gggcgtcccg cctgctgtgg cctccctgga ctttgccact
8101 gagccttaca atgctgcccg gccctacagc gtggccctgc tcagtgtccc cgaggccgcc
8161 cggacggaag cagggaagcc agaaagcagc acccccacag gcgagccagg ccccatggca
8221 tccagccctg agcccgctgt ggccaaggga ggcttcctga gcttccttga ggccaacatg
8281 ttcagcgtca tcatccccat gtgcctggta cttctgctcc tggcgctcat cctgcccctg
8341 ctcttctacc tccgaaaacg caacaagacg ggcaagcatg acgtccaggt cctgactgcc
8401 aagccccgca acggcctggc tggtgacacc gagacctttc gcaaggtgga gccaggccag
8461 gccatcccgc tcacagctgt gcctggccag gggccccctc caggaggcca gcctgaccca
8521 gagctgctgc agttctgccg gacacccaac cctgccctta agaatggcca gtactgggtg
8581 ggcggccgct cgagtctaga gggcccgcgg ttcgaacaaa aactcatctc agaagaggat
8641 ctgaatatgc ataccggtca tcatcaccat caccattgag tttaaacccg ctgatcagcc
8701 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
8761 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
8821 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
8881 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
8941 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
9001 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
9061 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
9121 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
9181 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
9241 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
9301 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
9361 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
9421 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
9481 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
9541 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
9601 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
9661 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
9721 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
9781 ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga
9841 caaggtgagg aactaaacca tggccaagcc tttgtctcaa gaagaatcca ccctcattga
9901 aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc
9961 agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg
10021 accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac
10081 ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg
10141 acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca
10201 gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg agggctaagc
10261 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct
10321 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
10381 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
10441 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
10501 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
10561 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
10621 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
10681 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
10741 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
10801 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
10861 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
10921 gaaagaacat gtgagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
10981 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
11041 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
11101 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
11161 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
11221 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
11281 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
11341 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
11401 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
11461 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
11521 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
11581 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
11641 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
11701 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
11761 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
11821 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
11881 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
11941 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
12001 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
12061 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
12121 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
12181 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
12241 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
12301 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
12361 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
12421 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
12481 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
12541 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
12601 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
12661 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
12721 ccacctgacg tc
//
