| 质粒名称: | pCS2+8NmCherry |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 34936 |
| 存储实验室: | Amro Hamdoun |
|
载体骨架基本信息 |
|
| 载体名称: | pCS2+ |
| 载体出品公司: | Turner and Weintraub (1994) |
| 空载体大小: | 4095 bp |
| 载体修饰: | 介绍了四种8bp切割限制性内切酶（AscI、PacI、FseI、AsiSI）。 |
| 载体类型: | 哺乳动物表达；海胆、斑马鱼、爪蟾 |
| 载体抗性: | 氨苄青霉素，100μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH10B |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 启动子: | SP6 |
| 标签/融合蛋白: | mCherry（主干上的N端子） |
|
文献引用方法 |
|
| 材料和方法部分: | pCS2+8NmCherry was a gift from Amro Hamdoun (Addgene plasmid # 34936 ; http://n2t.net/addgene:34936 ; RRID:Addgene_34936) |
| 参考文献部分: | Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR) Efflux Transporters. Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A. J Biol Chem. 2012 Nov 2. 10.1074/jbc.M112.424879 PubMed 23124201 |
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pCS2+8NmCherry载体质粒基本信息
pCS2+8NmCherry质粒图谱载体图谱和pCS2+8NmCherry载体序列质粒序列多克隆位点信息
pCS2+8NmCherry质粒载体简介
pCS2+8NmCherry质粒序列载体序列
一下序列为质粒部分序列
LOCUS pCS2+8NmCherry 899 bp DNA linear UNA 15-DEC-2025
DEFINITION natural linear DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE natural DNA sequence
ORGANISM unspecified
REFERENCE 1 (bases 1 to 899)
AUTHORS Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A
TITLE Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR)
Efflux Transporters.
JOURNAL J Biol Chem. 2012 Nov 2.
PUBMED 23124201
REFERENCE 2 (bases 1 to 899)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 15, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J Biol
Chem. 2012 Nov 2."
COMMENT Sequence Label: pCS2+8NmCherry
FEATURES Location/Qualifiers
source 1..899
/mol_type="genomic DNA"
/organism="unspecified"
promoter 41..59
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 41..58
/label=SP6
/note="SP6 promoter, forward primer"
CDS 106..813
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(251..269)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 519..538
/label=mCherry-F
/note="mCherry, forward primer"
ORIGIN
1 gggaaccgcc attctgcctg gggacgtcgg agcaagcttg atttaggtga cactatagaa
61 tacaagctac ttgttctttt tgcaggatcc actagtcatc gattgatggt gagcaagggc
121 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
181 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
241 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
301 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
361 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
421 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
481 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
541 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
601 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
661 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
721 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
781 cactccaccg gcggcatgga cgagctgtac aaggaattcg gagccggcgc gccattaatt
841 aaaggccggc cagcgatcgc caaggcctct cgagcctcta gaactatagt gagtcgtat
//
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