| 质粒名称: | p11L2w |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 37331 |
| 存储实验室: | John Schiller |
|
载体骨架基本信息 |
|
| 载体名称: | custom |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | 5974 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 载体抗性: | 卡那霉素，50μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 插入基因名称: | HPV11 L2 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | EF1a |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | p11L2w was a gift from John Schiller (Addgene plasmid # 37331 ; http://n2t.net/addgene:37331 ; RRID:Addgene_37331) |
| 参考文献部分: | Factors influencing subcellular localization of the human papillomavirus L2 minor structural protein. Kieback E, Muller M. Virology. 2006 Feb 5;345(1):199-208. Epub 2005 Oct 27. 10.1016/j.virol.2005.09.047 PubMed 16257028 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS p11L2w 5974 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS p11L2w
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5974)
AUTHORS Kieback E, Muller M
TITLE Factors influencing subcellular localization of the human
papillomavirus L2 minor structural protein.
JOURNAL Virology. 2006 Feb 5;345(1):199-208. Epub 2005 Oct 27.
PUBMED 16257028
REFERENCE 2 (bases 1 to 5974)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5974
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 113..1291
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 344..1282
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 1239..1259
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
misc_feature 2791..3379
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(2844..2864)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3262..3273)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
promoter complement(3401..3418)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
polyA_signal 3436..3557
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3447..3466)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 3501..3520
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
rep_origin 3745..4333
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4234..4253
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind complement(4417..4437)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 4422..4751
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4602..4737
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4664..4683
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 4799..4846
/label=EM7 promoter
/note="synthetic bacterial promoter "
promoter 4838..4856
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 4866..5660
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4920..4939)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 5530..5549
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(5718..5737)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 5762..5809
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
1 ccgctaccgg actcagatct cgagctcaag cttgcaaaga tggataaagt tttaaacaga
61 gaggaatctt tgcagctaat ggaccttgta ggtcttgaaa ggagtgggaa ttggctccgg
121 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
181 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
241 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
301 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
361 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
421 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
481 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
541 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
601 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
661 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
721 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
781 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
841 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
901 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
961 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
1021 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
1081 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
1141 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
1201 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
1261 aagttttttt cttccatttc aggtgtcgtg aggaattctc tagagatccc tcgacctcga
1321 gatccatcct gtacggaagt gttacttctg ctctaaaagc tgcggaattg tacccgcggc
1381 cgctctagat gaagcccaga gcccggcgga gaaagcgggc cagcgccacc cagctgtacc
1441 agacctgcaa ggccaccggc acctgccccc ctgacgtgat ccccaaggtg gagcacacca
1501 ccatcgccga ccagatcctg aagtggggca gcctgggcgt gttcttcggc ggcctgggca
1561 tcggcaccgg cgccggcagc ggcggccggg ccggctacat ccccctgggc agcagcccta
1621 agcccgccat caccgggggc cctgccgcca gaccccccgt gctggtggag cctgtggccc
1681 ccagcgaccc cagcatcgtg agtctgatcg aggagagcgc catcatcaac gccggcgccc
1741 ctgaggtggt gcctcctacc cagggcggct tcaccatcac cagcagcgag agcaccaccc
1801 ctgccatcct ggacgtgagc gtgaccaacc acaccaccac cagcgtgttc cagaaccccc
1861 tgttcaccga gcctagcgtg atccagcccc agcctcccgt ggaggccagc ggccacatcc
1921 tgatcagcgc ccctaccatc accagccagc acgtggagga catccccctg gacaccttcg
1981 tggtgagcag cagcgacagc ggccccacca gcagcacccc tctgccccgg gccttcccca
2041 gacctcgggt gggcctgtac agcagagccc tgcagcaggt gcaggtgacc gaccccgcct
2101 tcctgagcac cccccagcgg ctggtgacct acgacaaccc tgtgtacgag ggcgaggacg
2161 tgagcctgca gttcacccac gagagcatcc acaacgcccc cgacgaggcc ttcatggata
2221 tcatcagact gcaccggccc gccatcacca gcagacgggg cctggtgcgc ttcagcagaa
2281 tcggccagcg gggcagcatg tacacccgga gcggccagca catcggcgcc agaattcact
2341 acttccagga catcagccct gtgacccagg ccgccgagga gatcgagctg caccccctgg
2401 tggccgccga gaacgacacc ttcgacatct acgccgagcc cttcgaccct atccccgacc
2461 ccgtgcagca cagcgtgacc cagagctacc tgaccagcac ccctaacacc ctgagccaga
2521 gctggggcaa caccaccgtg cccctgagca tccccagcga ctggttcgtg cagagcggcc
2581 ccgacatcac cttccctacc gctagcatgg gcaccccctt cagccccgtg acccctgccc
2641 tgcccaccgg ccccgtgttc atcaccggca gcgacttcta cctgcaccct acctggtact
2701 tcgcccggcg gagacggaag cggatccccc tgttcttcac cgacgtggcc gcctaagctt
2761 atcgataccg tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
2821 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
2881 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
2941 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
3001 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
3061 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
3121 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
3181 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
3241 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
3301 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
3361 ctttgggccg cctccccgca tcgataccgt cggccgcttc cctttagtga gggttaatgc
3421 ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
3481 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
3541 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
3601 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gaattcttaa
3661 ttaactagcc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
3721 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
3781 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
3841 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
3901 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
3961 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
4021 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
4081 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
4141 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
4201 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
4261 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
4321 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
4381 ttttgctcac atgttcttaa ttaagctgta cactgtggaa tgtgtgtcag ttagggtgtg
4441 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
4501 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
4561 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
4621 ccagttccgc ccattctccg ccccatgact gactaatttt ttttatttat gcagaggccg
4681 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
4741 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt
4801 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgactcac tataggaggg
4861 ccaccatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
4921 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
4981 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
5041 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
5101 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
5161 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
5221 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
5281 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
5341 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc
5401 ccgacggcga ggatctcgtc gtgacacatg gcgatgcctg cttgccgaat atcatggtgg
5461 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
5521 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
5581 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
5641 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgaattc
5701 gctagaggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
5761 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
5821 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
5881 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
5941 gcagccaacg tcggggcggc aggccctgcc atag
//
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