| 质粒名称: | pLVX-EF1alpha-SARS-CoV-2-nsp12-2xStrep-IRES-Puro |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 141378 |
| 存储实验室: | Nevan Krogan |
|
载体骨架基本信息 |
|
| 载体名称: | pLVX-EF1alpha-IRES-Puro |
| 载体出品公司: | Takara Bio |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 8825 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达,慢病毒 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 30 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | SARS-CoV-2-nsp12 |
| 物种来源: | SARS-CoV-2 |
| 插入大小: | -- |
| 基因突变: | human codon optimized |
| GenBank编号 | -- |
| 启动子 | EF1alpha |
| 标签/融合蛋白: | 2xStrep（插接件上的C端子） |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLVX-EF1alpha-SARS-CoV-2-nsp12-2xStrep-IRES-Puro was a gift from Nevan Krogan (Addgene plasmid # 141378 ; http://n2t.net/addgene:141378 ; RRID:Addgene_141378) |
| 参考文献部分: | A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug Targets and Potential Drug Repurposing. Gordon DE, et al.. BioRxiv March 2020 10.1101/2020.03.22.002386 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-EF1alpha-SARS-CoV-2-nsp12-2xStrep-IRES-Puro 11712 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Lentiviral expression of SARS-CoV-2 protein; transient expression
and generate lentivirus.
ACCESSION .
VERSION .
KEYWORDS pLVX-EF1alpha-SARS-CoV-2-nsp12-2xStrep-IRES-Puro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11712)
AUTHORS Gordon DE, et al.
TITLE A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug
Targets and Potential Drug Repurposing
JOURNAL BioRxiv March 2020
REFERENCE 2 (bases 1 to 11712)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11712
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1721..1765
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2028..2143
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
promoter 2338..3519
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 2568..3510
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 3467..3487
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
CDS 6367..6390
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
CDS 6427..6450
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
misc_feature 6462..7035
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(6629..6646)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 6856..6875
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 7068..7667
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(7068..7087)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 7564..7584
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 7681..8269
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(7734..7754)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(8152..8163)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 8476..9109
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
primer_bind complement(9237..9253)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(9237..9253)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(9250..9272)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 9261..9277
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(9285..9315)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9330..9351
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(9468..9485)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(9639..10227)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(9719..9738)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(10398..11258)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 11021..11040
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(11259..11363)
/gene="bla"
/label=AmpR promoter
polyA_signal 11411..11545
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(11448..11467)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 11502..11521
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc
3541 gccgccacca tgtcagcaga cgcacaaagt tttctgaacc gggtatgcgg agtcagtgcg
3601 gcgaggctta cgccctgtgg tacgggaaca tcaacggacg ttgtttaccg cgccttcgac
3661 atatacaatg ataaagtcgc cggcttcgca aagttcctta aaacgaattg ctgtaggttc
3721 caagaaaagg acgaggatga taatttgata gactcctact tcgtggtcaa acgacacacg
3781 ttttcaaatt atcagcacga ggaaacgata tataatcttc ttaaagactg ccctgcagtg
3841 gcaaaacacg acttctttaa gttccgcatt gacggggata tggtccccca tatctcccga
3901 cagagattga cgaaatatac aatggcggat ctggtgtacg ccctgagaca cttcgatgag
3961 ggcaattgtg atacactcaa ggagattctc gtgacctaca attgttgcga tgatgactat
4021 ttcaataaga aagactggta cgacttcgtc gaaaaccctg atatattgag ggtgtacgca
4081 aacctgggtg aacgagtaag acaggcactt ctgaaaaccg tccaattctg cgacgctatg
4141 agaaacgcgg gcattgttgg tgtgcttacg ctggataacc aagacctcaa tggtaattgg
4201 tacgattttg gggacttcat ccaaaccact cctggctcag gcgttcccgt ggtagacagc
4261 tactattctt tgttgatgcc aattcttaca ctcacaaggg ccctgaccgc tgagtcacac
4321 gtcgatacgg acttgaccaa gccctacatc aagtgggatc ttctgaagta cgactttacc
4381 gaggaacgat tgaagctttt tgatcgctac tttaaatact gggatcagac gtaccaccca
4441 aactgcgtga attgcctgga tgaccgatgt atactccatt gcgcgaactt taacgttttg
4501 ttctctacag tatttcctcc gacgagcttc ggaccgcttg tgagaaagat attcgtcgac
4561 ggcgtcccat ttgttgtcag cactggttac cactttcgcg agttgggggt ggtccacaat
4621 caggatgtca atttgcattc ctccaggctt tcttttaaag agttgcttgt ttatgccgct
4681 gaccccgcga tgcatgcagc ctctgggaat cttttgctgg acaaaaggac gacgtgcttt
4741 agcgttgctg cccttacaaa taacgtagca tttcaaaccg taaaacccgg taattttaat
4801 aaagattttt atgattttgc agtctctaag ggttttttca aggaggggag tagtgtagag
4861 cttaaacatt tttttttcgc tcaggatggt aacgctgcga tcagcgacta cgactactat
4921 cgatacaatc tcccgacgat gtgtgatatc aggcagttgt tgttcgttgt agaagtggtt
4981 gataagtatt ttgactgtta tgacggcggc tgtatcaatg ccaaccaggt cattgtgaat
5041 aacctcgata aatctgccgg gttccccttt aacaagtggg ggaaggcgcg actgtactac
5101 gacagcatgt cttacgaaga tcaggacgca ctctttgcat acacaaagag gaatgtgata
5161 ccgactataa ctcaaatgaa ccttaagtat gcaatcagcg cgaaaaatcg ggctaggact
5221 gttgccggag ttagcatatg tagtaccatg acaaaccgac agttccacca gaagttgctg
5281 aaatcaattg ctgcgacccg gggtgcgact gtagtaatcg ggacatcaaa gttctacggc
5341 gggtggcata acatgctcaa gacggtctac tcagacgttg agaatccgca tcttatgggt
5401 tgggattatc caaagtgtga cagggcgatg cccaacatgt tgcggataat ggcctccttg
5461 gtacttgcac ggaaacatac aacctgctgt agtttgtcac accgatttta ccgactggca
5521 aacgaatgtg ctcaggttct cagtgaaatg gtaatgtgtg gcggctccct ctacgttaag
5581 cccggtggca caagctctgg agacgcaact actgcctatg caaacagtgt attcaacatc
5641 tgccaggctg tcacggccaa tgttaacgca ttgctttcca ctgacggaaa taagattgca
5701 gacaaatacg tgagaaattt gcaacataga ctctacgaat gcctctatcg caatcgcgat
5761 gttgatacgg acttcgttaa tgagttctat gcttacttgc gaaagcactt ctccatgatg
5821 attctgtcag acgacgctgt agtatgcttt aattcaacgt atgcctccca gggtctggtc
5881 gcgtccataa aaaattttaa aagcgtcctg tattaccaaa ataacgtttt catgagcgaa
5941 gcgaaatgtt ggaccgagac tgatcttact aagggccctc atgagttctg ctcacaacac
6001 actatgttgg tcaagcaggg cgacgattac gtgtatcttc cttatcccga tcctagtagg
6061 attcttggag caggttgctt cgtggatgat atcgtcaaaa cggacggcac cctcatgata
6121 gagaggttcg tctctttggc tatagacgcg tatccgctta ctaaacatcc taaccaagaa
6181 tacgcggatg tattccatct ttatctgcag tatatcagaa agttgcatga tgagctgacc
6241 ggtcacatgt tggacatgta tagcgtgatg cttaccaatg acaatacttc acggtactgg
6301 gaacctgaat tttacgaggc gatgtatacg cctcacaccg tcctgcagct cgaaggcggc
6361 gggggatgga gccatccaca attcgagaaa ggcggtggtt caggaggagg tagcgggggt
6421 ggatcatggt cacatccgca gtttgaaaag taaggatccc gcccctctcc ctcccccccc
6481 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
6541 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
6601 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
6661 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
6721 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
6781 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
6841 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
6901 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
6961 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
7021 aaaacacgat gataagcttg ccacaaccca caaggagacg accttccatg accgagtaca
7081 agcccacggt gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc accctcgccg
7141 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga cccggaccgc cacatcgagc
7201 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
7261 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
7321 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
7381 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
7441 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
7501 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
7561 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
7621 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctagacg cgtctggaac
7681 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
7741 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
7801 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
7861 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
7921 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
7981 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
8041 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
8101 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
8161 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
8221 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattaat
8281 tctgcagtcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
8341 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
8401 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
8461 aagaaaagag gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
8521 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag
8581 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
8641 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
8701 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
8761 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
8821 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
8881 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
8941 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
9001 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
9061 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
9121 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
9181 ccttgacatt gctagcgttt accgtcgacc tctagctaga gcttggcgta atcatggtca
9241 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
9301 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
9361 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
9421 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
9481 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
9541 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
9601 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
9661 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
9721 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
9781 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
9841 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
9901 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
9961 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
10021 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
10081 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
10141 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
10201 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
10261 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
10321 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
10381 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
10441 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
10501 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
10561 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
10621 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
10681 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
10741 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
10801 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
10861 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
10921 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
10981 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
11041 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
11101 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
11161 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
11221 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
11281 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
11341 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
11401 tcgggagatc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
11461 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
11521 caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa
11581 acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac
11641 ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac
11701 aaacttagta gt
//
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