| 质粒名称: | xCas9(3.7)-ABE(7.10) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 108382 |
| 存储实验室: | David Liu |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | xCas9(3.7)-ABE(7.10),TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nxCas9(3.7)-NLS |
| 物种来源: | Synthetic |
| 插入大小: | 5361 bp |
| 基因突变: | A262T, R324L, S409I, E480K, E543D, M694I, E1219V |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | NLS（插入件上的N端子） |
| 克隆方法: | Gibson 克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | CMV Forward |
| 3' 测序引物 | M13 |
|
文献引用方法 |
|
| 材料和方法部分: | xCas9(3.7)-ABE(7.10) was a gift from David Liu (Addgene plasmid # 108382 ; http://n2t.net/addgene:108382 ; RRID:Addgene_108382) |
| 参考文献部分: | Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Hu JH, Miller SM, Geurts MH, Tang W, Chen L, Sun N, Zeina CM, Gao X, Rees HA, Lin Z, Liu DR. Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155. 10.1038/nature26155 PubMed 29512652 |
xCas9(3.7)-ABE(7.10)
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
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xCas9(3.7)-ABE(7.10)载体质粒基本信息
xCas9(3.7)-ABE(7.10)质粒图谱载体图谱和xCas9(3.7)-ABE(7.10)载体序列质粒序列多克隆位点信息
-ABE(7.10)质粒图谱.png)
xCas9(3.7)-ABE(7.10)质粒载体简介
xCas9(3.7)-ABE(7.10)质粒序列载体序列
LOCUS xCas9(3.7)-ABE(7.10) 8760 bp ds-DNA circular SYN 09-NOV-2018
DEFINITION Mammalian expression vector for xCas9(3.7)-ABE.
ACCESSION .
VERSION .
KEYWORDS xCas9(3.7)-ABE(7.10)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8760)
AUTHORS Hu JH, Miller SM, Geurts MH, Tang W, Chen L, Sun N, Zeina CM, Gao X,
Rees HA, Lin Z, Liu DR
TITLE Evolved Cas9 variants with broad PAM compatibility and high DNA
specificity.
JOURNAL Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155.
PUBMED 29512652
REFERENCE 2 (bases 1 to 8760)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Nov 9, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8760
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 19..398
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 399..602
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 553..573
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 644..663
/label=T7
/note="T7 promoter, forward primer"
promoter 644..662
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 1867..5967
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDTKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKLYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGIIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEKVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGDQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFIQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGVLQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 6013..6033
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 6042..6059
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(6082..6099)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 6088..6312
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(6383..6399)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6383..6399)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6396..6418)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6407..6423
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6431..6461)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6476..6497
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6614..6631)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6785..7373)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6865..6884)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7544..8404)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8167..8186
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8405..8509)
/gene="bla"
/label=AmpR promoter
primer_bind complement(8588..8607)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
61 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
121 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
181 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
241 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
301 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
361 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
421 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
481 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
541 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
601 ctggtttagt gaaccgtcag atccgctaga gatccgcggc cgctaatacg actcactata
661 gggagagccg ccaccatgtc cgaagtcgag ttttcccatg agtactggat gagacacgca
721 ttgactctcg caaagagggc ttgggatgaa cgcgaggtgc ccgtgggggc agtactcgtg
781 cataacaatc gcgtaatcgg cgaaggttgg aataggccga tcggacgcca cgaccccact
841 gcacatgcgg aaatcatggc ccttcgacag ggagggcttg tgatgcagaa ttatcgactt
901 atcgatgcga cgctgtacgt cacgcttgaa ccttgcgtaa tgtgcgcggg agctatgatt
961 cactcccgca ttggacgagt tgtattcggt gcccgcgacg ccaagacggg tgccgcaggt
1021 tcactgatgg acgtgctgca tcacccaggc atgaaccacc gggtagaaat cacagaaggc
1081 atattggcgg acgaatgtgc ggcgctgttg tccgactttt ttcgcatgcg gaggcaggag
1141 atcaaggccc agaaaaaagc acaatcctct actgactctg gtggttcttc tggtggttct
1201 agcggcagcg agactcccgg gacctcagag tccgccacac ccgaaagttc tggtggttct
1261 tctggtggtt cttccgaagt cgagttttcc catgagtact ggatgagaca cgcattgact
1321 ctcgcaaaga gggctcgaga tgaacgcgag gtgcccgtgg gggcagtact cgtgctcaac
1381 aatcgcgtaa tcggcgaagg ttggaatagg gcaatcggac tccacgaccc cactgcacat
1441 gcggaaatca tggcccttcg acagggaggg cttgtgatgc agaattatcg acttatcgat
1501 gcgacgctgt acgtcacgtt tgaaccttgc gtaatgtgcg cgggagctat gattcactcc
1561 cgcattggac gagttgtatt cggtgttcgc aacgccaaga cgggtgccgc aggttcactg
1621 atggacgtgc tgcattaccc aggcatgaac caccgggtag aaatcacaga aggcatattg
1681 gcggacgaat gtgcggcgct gttgtgttac ttttttcgca tgcccaggca ggtctttaac
1741 gcccagaaaa aagcacaatc ctctactgac tctggtggtt cttctggtgg ttctagcggc
1801 agcgagactc ccgggacctc agagtccgcc acacccgaaa gttctggtgg ttcttctggt
1861 ggttctgaca agaagtactc cattgggctc gctatcggca caaacagcgt cggctgggcc
1921 gtcattacgg acgagtacaa ggtgccgagc aaaaaattca aagttctggg caataccgat
1981 cgccacagca taaagaagaa cctcattggc gccctcctgt tcgactccgg ggagacggcc
2041 gaagccacgc ggctcaaaag aacagcacgg cgcagatata cccgcagaaa gaatcggatc
2101 tgctacctgc aggagatctt tagtaatgag atggctaagg tggatgactc tttcttccat
2161 aggctggagg agtccttttt ggtggaggag gataaaaagc acgagcgcca cccaatcttt
2221 ggcaatatcg tggacgaggt ggcgtaccat gaaaagtacc caaccatata tcatctgagg
2281 aagaagcttg tagacagtac tgataaggct gacttgcggt tgatctatct cgcgctggcg
2341 catatgatca aatttcgggg acacttcctc atcgaggggg acctgaaccc agacaacagc
2401 gatgtcgaca aactctttat ccaactggtt cagacttaca atcagctttt cgaagagaac
2461 ccgatcaacg catccggagt tgacgccaaa gcaatcctga gcgctaggct gtccaaatcc
2521 cggcggctcg aaaacctcat cgcacagctc cctggggaga agaagaacgg cctgtttggt
2581 aatcttatcg ccctgtccct cgggctgacc cccaacttta aatctaactt cgacctggcc
2641 gaagatacca agcttcaact gagcaaagac acctacgatg atgatctcga caatctgctg
2701 gcccagatcg gcgaccagta cgcagacctt tttttggcgg caaagaacct gtcagacgcc
2761 attctgctga gtgatattct gcgagtgaac acggagatca ccaaagctcc gctgagcgct
2821 agtatgatca agctctatga tgagcaccac caagacttga ctttgctgaa ggcccttgtc
2881 agacagcaac tgcctgagaa gtacaaggaa attttcttcg atcagtctaa aaatggctac
2941 gccggataca ttgacggcgg agcaagccag gaggaatttt acaaatttat taagcccatc
3001 ttggaaaaaa tggacggcac cgaggagctg ctggtaaagc ttaacagaga agatctgttg
3061 cgcaaacagc gcactttcga caatggaatc atcccccacc agattcacct gggcgaactg
3121 cacgctatcc tcaggcggca agaggatttc tacccctttt tgaaagataa cagggaaaag
3181 attgagaaaa tcctcacatt tcggataccc tactatgtag gccccctcgc ccggggaaat
3241 tccagattcg cgtggatgac tcgcaaatca gaagagacca tcactccctg gaacttcgag
3301 aaagtcgtgg ataagggggc ctctgcccag tccttcatcg aaaggatgac taactttgat
3361 aaaaatctgc ctaacgaaaa ggtgcttcct aaacactctc tgctgtacga gtacttcaca
3421 gtttataacg agctcaccaa ggtcaaatac gtcacagaag ggatgagaaa gccagcattc
3481 ctgtctggag atcagaagaa agctattgtg gacctcctct tcaagacgaa ccggaaagtt
3541 accgtgaaac agctcaaaga agactatttc aaaaagattg aatgtttcga ctctgttgaa
3601 atcagcggag tggaggatcg cttcaacgca tccctgggaa cgtatcacga tctcctgaaa
3661 atcattaaag acaaggactt cctggacaat gaggagaacg aggacattct tgaggacatt
3721 gtcctcaccc ttacgttgtt tgaagatagg gagatgattg aagaacgctt gaaaacttac
3781 gctcatctct tcgacgacaa agtcatgaag cagctcaaga ggcgccgata tacaggatgg
3841 gggcggctgt caagaaaact gatcaatggg atccgagaca agcagagtgg aaagacaatc
3901 ctggattttc ttaagtccga tggatttgcc aaccggaact tcattcagtt gatccatgat
3961 gactctctca cctttaagga ggacatccag aaagcacaag tttctggcca gggggacagt
4021 cttcacgagc acatcgctaa tcttgcaggt agcccagcta tcaaaaaggg aatactgcag
4081 accgttaagg tcgtggatga actcgtcaaa gtaatgggaa ggcataagcc cgagaatatc
4141 gttatcgaga tggcccgaga gaaccaaacc acccagaagg gacagaagaa cagtagggaa
4201 aggatgaaga ggattgaaga gggtataaaa gaactggggt cccaaatcct taaggaacac
4261 ccagttgaaa acacccagct tcagaatgag aagctctacc tgtactacct gcagaacggc
4321 agggacatgt acgtggatca ggaactggac atcaatcggc tctccgacta cgacgtggat
4381 catatcgtgc cccagtcttt tctcaaagat gattctattg ataataaagt gttgacaaga
4441 tccgataaaa acagagggaa gagtgataac gtcccctcag aagaagttgt caagaaaatg
4501 aaaaattatt ggcggcagct gctgaacgcc aaactgatca cacaacggaa gttcgataat
4561 ctgactaagg ctgaacgagg tggcctgtct gagttggata aagccggttt catcaaaagg
4621 cagcttgttg agacacgcca gatcaccaag cacgtggccc aaattctcga ttcacgcatg
4681 aacaccaagt acgatgaaaa tgacaaactg attcgagagg tgaaagttat tactctgaag
4741 tctaagctgg tctcagattt cagaaaggac tttcagtttt ataaggtgag agagatcaac
4801 aattaccacc atgcgcatga tgcctacctg aatgcagtgg taggcactgc acttatcaaa
4861 aaatatccca agcttgaatc tgaatttgtt tacggagact ataaagtgta cgatgttagg
4921 aaaatgatcg caaagtctga gcaggaaata ggcaaggcca ccgctaagta cttcttttac
4981 agcaatatta tgaatttttt caagaccgag attacactgg ccaatggaga gattcggaag
5041 cgaccactta tcgaaacaaa cggagaaaca ggagaaatcg tgtgggacaa gggtagggat
5101 ttcgcgacag tccggaaggt cctgtccatg ccgcaggtga acatcgttaa aaagaccgaa
5161 gtacagaccg gaggcttctc caaggaaagt atcctcccga aaaggaacag cgacaagctg
5221 atcgcacgca aaaaagattg ggaccccaag aaatacggcg gattcgattc tcctacagtc
5281 gcttacagtg tactggttgt ggctaaagtg gagaaaggga agtctaaaaa actcaaaagc
5341 gtcaaggaac tgctgggcat cacaatcatg gagcgatcaa gcttcgaaaa aaaccccatc
5401 gactttctcg aggcgaaagg atataaagag gtcaaaaaag acctcatcat taagcttccc
5461 aagtactctc tctttgagct tgaaaacggc cggaaacgaa tgctcgctag tgcgggcgtg
5521 ctgcagaaag gtaacgagct ggcactgccc tctaaatacg ttaatttctt gtatctggcc
5581 agccactatg aaaagctcaa agggtctccc gaagataatg agcagaagca gctgttcgtg
5641 gaacaacaca aacactacct tgatgagatc atcgagcaaa taagcgaatt ctccaaaaga
5701 gtgatcctcg ccgacgctaa cctcgataag gtgctttctg cttacaataa gcacagggat
5761 aagcccatca gggagcaggc agaaaacatt atccacttgt ttactctgac caacttgggc
5821 gcgcctgcag ccttcaagta cttcgacact accatagaca gaaagcggta cacctctaca
5881 aaggaggtcc tggacgccac actgattcat cagtcaatta cggggctcta tgaaacaaga
5941 atcgacctct ctcagctcgg tggagacgag ggagctgata agcgcaccgc cgatggttcc
6001 gagttcgaaa gccccaagaa gaagaggaaa gtctaaccgg tcatcatcac catcaccatt
6061 gagtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
6121 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
6181 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
6241 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
6301 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctcgataccg tcgacctcta
6361 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
6421 caattccaca caacatacga gccggaagca taaagtgtaa agcctagggt gcctaatgag
6481 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
6541 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
6601 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
6661 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
6721 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
6781 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
6841 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
6901 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
6961 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
7021 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
7081 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
7141 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
7201 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
7261 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
7321 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
7381 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
7441 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
7501 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
7561 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
7621 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
7681 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
7741 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
7801 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
7861 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
7921 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
7981 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
8041 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
8101 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
8161 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
8221 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
8281 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
8341 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
8401 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
8461 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
8521 gaaaagtgcc acctgacgtc gacggatcgg gagatcgatc tcccgatccc ctagggtcga
8581 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
8641 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
8701 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
//
