| 质粒名称: | pC1-REX-YFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 48247 |
| 存储实验室: | Vsevolod Belousov |
|
载体骨架基本信息 |
|
| 载体名称: | pC1 |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 3982 bp |
| 完整质粒大小: | 5370 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | REX-YFP |
| 物种来源: | Synthetic |
| 插入大小: | 1389 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | NheI (未破坏) |
| 3' 克隆位点: | HindIII (未破坏) |
| 5' 测序引物 | cggtgggaggtctatataag |
| 3' 测序引物 | tgggaggttttttaaagcaag |
|
文献引用方法 |
|
| 材料和方法部分: | pC1-REX-YFP was a gift from Vsevolod Belousov (Addgene plasmid # 48247 ; http://n2t.net/addgene:48247 ; RRID:Addgene_48247) |
| 参考文献部分: | Genetically encoded fluorescent indicator for imaging NAD/NADH ratio changes in different cellular compartments. Bilan DS, Matlashov ME, Gorokhovatsky AY, Schultz C, Enikolopov G, Belousov VV. Biochim Biophys Acta. 2013 Nov 25. pii: S0304-4165(13)00519-9. doi: 10.1016/j.bbagen.2013.11.018. 10.1016/j.bbagen.2013.11.018 PubMed 24286672 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pC1-REX-YFP 5370 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Genetically encoded fluorescent indicator for measuring NAD/NADH
ratio..
ACCESSION .
VERSION .
KEYWORDS pC1-REX-YFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5370)
AUTHORS Bilan DS, Matlashov ME, Gorokhovatsky AY, Schultz C, Enikolopov G,
Belousov VV
TITLE Genetically encoded fluorescent indicator for imaging NAD/NADH ratio
changes in different cellular compartments.
JOURNAL Biochim Biophys Acta. 2013 Nov 25. pii: S0304-4165(13)00519-9. doi:
10.1016/j.bbagen.2013.11.018.
PUBMED 24286672
REFERENCE 2 (bases 1 to 5370)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5370
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 519..539
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 1067..1088
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(1197..1218)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1458..1477)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
polyA_signal 2158..2279
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2195..2214)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2249..2268
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(2286..2741)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2423..2444)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2635..2654
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2768..2872
/gene="bla"
/label=AmpR promoter
promoter 2874..3231
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(2897..2917)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 3082..3217
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3144..3163
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3266..4060
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3320..3339)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3930..3949
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(4248..4267)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 4292..4339
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4668..5256
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5157..5176
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca
601 ccatgaaggt ccccgaagcg gccatcagcc gcctgatcac ctacctccgc atcctggagg
661 agctggaggc ccagggggtt caccggacga gctccgagca gcttggggag ctcgcccagg
721 tcacggcctt ccaggtgcgg aaggaccttt cctacttcgg ctcctacggg acgcgcgggg
781 tggggtacac cgtccccgtc ctgaagcggg agctccgcca catcctgggg ctcaaccgct
841 ccgccggcta caacagcgac aacgtctata tcatggccga caagcagaag aacggcatca
901 aggccaactt caagatccgc cacaacgtcg aggacggcag cgtgcagctc gccgaccact
961 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
1021 gcttccagtc cgtcctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
1081 agttcgtgac cgccgccggg atcactcccg gcatggacga gctgaacaac gtggatggcg
1141 gtagcggtgg caccggcagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
1201 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
1261 atgccaccta cggcaagctg accctgaagc tgatctgcac caccggcaag ctgcccgtgc
1321 cctggcccac cctcgtgacc accctcggct acggcctgaa gtgcttcgcc cgctaccccg
1381 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
1441 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
1501 gcgacaccct ggtgaaccgc atcgagctga agggcatcgg cttcaaggag gacggcaaca
1561 tcctggggca caagctggag tacaacggca ccaagtgggg cctgtgcatc gtgggcatgg
1621 gccggctcgg gagcgccctg gccgactacc cggggttcgg ggagagcttt gagctcaggg
1681 gcttctttga cgtggacccg gagaaggtgg gccgcccggt gcggggcggg gtcattgagc
1741 acgtggacct cctgccccag cgggttcccg gccgcattga gatcgccctc ctcaccgtgc
1801 cccgggaagc ggcccagaag gcggcggacc tgttggtggc ggcggggatc aaggggatcc
1861 tgaacttcgc cccggtggtc ctcgaggtgc ccaaggaggt ggcggtggag aacgtggact
1921 tcttggcggg gctcacgcgg ctttctttcg ctatcctgaa ccccaagtgg cgggaagaga
1981 tgatggggtg aagcttcgaa ttctgcagtc gacggtaccg cgggcccggg atccaccgga
2041 tctagataac tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
2101 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
2161 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
2221 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
2281 cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
2341 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
2401 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
2461 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
2521 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
2581 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
2641 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
2701 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg
2761 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
2821 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc
2881 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
2941 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
3001 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
3061 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
3121 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
3181 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca
3241 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
3301 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
3361 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
3421 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac
3481 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
3541 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
3601 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
3661 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
3721 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
3781 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
3841 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
3901 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
3961 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
4021 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
4081 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
4141 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
4201 ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag
4261 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
4321 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
4381 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
4441 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
4501 atagcctcag gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
4561 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
4621 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
4681 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
4741 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
4801 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
4861 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
4921 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
4981 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
5041 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
5101 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
5161 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
5221 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
5281 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
5341 tctgtggata accgtattac cgccatgcat
//
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