pGL4.11[luc2P]

价格:1500元

联系方式:I47-825O-882O

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pGL4.11载体基本信息

出品公司: Promega
载体名称: pGL4.11 ; pGL4.11[luc2P]
质粒类型: 信号通路报告载体;哺乳动物载体;萤火虫荧光素酶报告载体
高拷贝/低拷贝: --
克隆方法: 限制性内切酶,多克隆位点
启动子:
载体大小: 4370 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: luc2P
载体抗性: 氨苄青霉素
筛选标记: 潮霉素(Hygromycin)
克隆菌株: TOP10等常规菌株
宿主细胞(系): HEK293等
备注: pGL4.11[luc2P]载体是信号通路报告载体,无启动子,含萤火虫荧光 素酶报告基因luc2P
产品目录号: E666A
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

pGL4.11载体质粒图谱和多克隆位点信息

pGL4.11载体图谱



pGL4.11 多克隆位点

pGL4.11 载体特征

pGL4.11载体简介

The pGL4.11[luc2P] Vector encodes the luciferase reporter gene luc2P (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been codon optimized for mammalian expression. The pGL4.11[luc2P] Vector is a basic vector with no promoter. However, it contains a multiple cloning region to allow cloning of a promoter of choice. The luc2P reporter gene contains hPEST, a protein destabilization sequence. The protein encoded by luc2P responds more quickly and with greater magnitude to changes in transcriptional activity than the luc2 gene, its more stable counterpart.

pGL4.11载体序列

LOCUS       AY738223                4370 bp    DNA     circular SYN 08-NOV-2004
DEFINITION  Firefly luciferase reporter vector pGL4.11[luc2P], complete
            sequence.
ACCESSION   AY738223
VERSION     AY738223.1  GI:55535621
KEYWORDS    .
SOURCE      Firefly luciferase reporter vector pGL4.11[luc2P]
  ORGANISM  Firefly luciferase reporter vector pGL4.11[luc2P]
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Almond,B., Chauvin,F. and Browning,J.
  TITLE     pGL4 Luciferase Reporter Vectors Technical Manual, TM259, Promega
            Corporation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   Almond,B., Chauvin,F. and Browning,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2004) Scientific Communications, Promega
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Firefly luciferase reporter vector
                     pGL4.11[luc2P]"
                     /mol_type="other DNA"
                     /db_xref="taxon:296660"
     misc_feature    1..70
                     /note="multiple cloning site region"
     CDS             100..1875
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase luc2P"
                     /protein_id="AAV52871.1"
                     /db_xref="GI:55535622"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                     AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
                     APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
                     FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
                     FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
                     LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
                     GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
                     RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
                     PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
                     AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAVNSHGFPPEVEEQAAGT
                     LPMSCAQESGMDRHPAACASARINV"
     misc_feature    1915..2136
                     /note="SV40 late poly(A) region"
     primer_bind     2204..2223
                     /note="reporter vector primer 4 (RVPrimer4)"
     rep_origin      2461
                     /note="ColE1-derived"
     gene            complement(3252..4112)
                     /gene="ampR"
     CDS             complement(3252..4112)
                     /gene="ampR"
                     /note="AmpR"
                     /codon_start=1
                     /transl_table=11
                     /product="synthetic beta-lactamase"
                     /protein_id="AAV52872.1"
                     /db_xref="GI:55535623"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     regulatory      4217..4370
                     /regulatory_class="polyA_signal_sequence"
                     /note="synthetic; transcriptional pause site"
     primer_bind     complement(4217..4370)
                     /note="reporter vector primer 3 (RVPrimer3)"
ORIGIN      
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggaagatgc caaaaacatt
      121 aagaagggcc cagcgccatt ctacccactc gaagacggga ccgccggcga gcagctgcac
      181 aaagccatga agcgctacgc cctggtgccc ggcaccatcg cctttaccga cgcacatatc
      241 gaggtggaca ttacctacgc cgagtacttc gagatgagcg ttcggctggc agaagctatg
      301 aagcgctatg ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag
      361 ttcttcatgc ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc agctaacgac
      421 atctacaacg agcgcgagct gctgaacagc atgggcatca gccagcccac cgtcgtattc
      481 gtgagcaaga aagggctgca aaagatcctc aacgtgcaaa agaagctacc gatcatacaa
      541 aagatcatca tcatggatag caagaccgac taccagggct tccaaagcat gtacaccttc
      601 gtgacttccc atttgccacc cggcttcaac gagtacgact tcgtgcccga gagcttcgac
      661 cgggacaaaa ccatcgccct gatcatgaac agtagtggca gtaccggatt gcccaagggc
      721 gtagccctac cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc
      781 ggcaaccaga tcatccccga caccgctatc ctcagcgtgg tgccatttca ccacggcttc
      841 ggcatgttca ccacgctggg ctacttgatc tgcggctttc gggtcgtgct catgtaccgc
      901 ttcgaggagg agctattctt gcgcagcttg caagactata agattcaatc tgccctgctg
      961 gtgcccacac tatttagctt cttcgctaag agcactctca tcgacaagta cgacctaagc
     1021 aacttgcacg agatcgccag cggcggggcg ccgctcagca aggaggtagg tgaggccgtg
     1081 gccaaacgct tccacctacc aggcatccgc cagggctacg gcctgacaga aacaaccagc
     1141 gccattctga tcacccccga aggggacgac aagcctggcg cagtaggcaa ggtggtgccc
     1201 ttcttcgagg ctaaggtggt ggacttggac accggtaaga cactgggtgt gaaccagcgc
     1261 ggcgagctgt gcgtccgtgg ccccatgatc atgagcggct acgttaacaa ccccgaggct
     1321 acaaacgctc tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc ctactgggac
     1381 gaggacgagc acttcttcat cgtggaccgg ctgaagagcc tgatcaaata caagggctac
     1441 caggtagccc cagccgaact ggagagcatc ctgctgcaac accccaacat cttcgacgcc
     1501 ggggtcgccg gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg
     1561 gaacacggta aaaccatgac cgagaaggag atcgtggact atgtggccag ccaggttaca
     1621 accgccaaga agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc
     1681 ggcaagttgg acgcccgcaa gatccgcgag attctcatta aggccaagaa gggcggcaag
     1741 atcgccgtga attctcacgg cttccctccc gaggtggagg agcaggccgc cggcaccctg
     1801 cccatgagct gcgcccagga gagcggcatg gatagacacc ctgctgcttg cgccagcgcc
     1861 aggatcaacg tctaaggccg cgactctaga gtcggggcgg ccggccgctt cgagcagaca
     1921 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     1981 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     2041 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     2101 ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccgtcgaccg
     2161 atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc
     2221 gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg
     2281 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     2341 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     2401 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2461 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2521 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2581 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2641 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2701 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2761 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2821 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2881 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     2941 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3001 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3061 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3121 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     3181 taaatcaatc taaagtatat atgagtaaac ttggtctgac agcggccgca aatgctaaac
     3241 cactgcagtg gttaccagtg cttgatcagt gaggcaccga tctcagcgat ctgcctattt
     3301 cgttcgtcca tagtggcctg actccccgtc gtgtagatca ctacgattcg tgagggctta
     3361 ccatcaggcc ccagcgcagc aatgatgccg cgagagccgc gttcaccggc ccccgatttg
     3421 tcagcaatga accagccagc agggagggcc gagcgaagaa gtggtcctgc tactttgtcc
     3481 gcctccatcc agtctatgag ctgctgtcgt gatgctagag taagaagttc gccagtgagt
     3541 agtttccgaa gagttgtggc cattgctact ggcatcgtgg tatcacgctc gtcgttcggt
     3601 atggcttcgt tcaactctgg ttcccagcgg tcaagccggg tcacatgatc acccatatta
     3661 tgaagaaatg cagtcagctc cttagggcct ccgatcgttg tcagaagtaa gttggccgcg
     3721 gtgttgtcgc tcatggtaat ggcagcacta cacaattctc ttaccgtcat gccatccgta
     3781 agatgctttt ccgtgaccgg cgagtactca accaagtcgt tttgtgagta gtgtatacgg
     3841 cgaccaagct gctcttgccc ggcgtctata cgggacaaca ccgcgccaca tagcagtact
     3901 ttgaaagtgc tcatcatcgg gaatcgttct tcggggcgga aagactcaag gatcttgccg
     3961 ctattgagat ccagttcgat atagcccact cttgcaccca gttgatcttc agcatctttt
     4021 actttcacca gcgtttcggg gtgtgcaaaa acaggcaagc aaaatgccgc aaagaaggga
     4081 atgagtgcga cacgaaaatg ttggatgctc atactcgtcc tttttcaata ttattgaagc
     4141 atttatcagg gttactagta cgtctctcaa ggataagtaa gtaatattaa ggtacgggag
     4201 gtattggaca ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt
     4261 gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac aaaacaaact
     4321 agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct
//

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