| 质粒名称: | pDGM3B |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 110809 |
| 存储实验室: | David Russell |
|
载体骨架基本信息 |
|
| 载体名称: | pDG |
| 载体出品公司: | -- |
| 空载体大小: | 21845 bp |
| 完整质粒大小: | 21851 bp |
| 载体修饰: | -- |
| 载体类型: |
AAV
|
| 筛选标记: | -- |
| 载体抗性: | 氨苄青霉素，100μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | AAV3B cap基因、AAV2 rep基因、腺病毒辅助基因 |
| 物种来源: | 腺病毒，腺相关病毒 |
| 插入大小: | 3086 bp |
| 基因突变: | -- |
| GenBank编号 | AF028705.1 |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | Sal1(未破坏) |
| 3’克隆位点: | Cla1 (未破坏) |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pDGM3B was a gift from David Russell (Addgene plasmid # 110809 ; http://n2t.net/addgene:110809 ; RRID:Addgene_110809) |
| 参考文献部分: | AAV-mediated gene targeting methods for human cells. Khan IF, Hirata RK, Russell DW. Nat Protoc. 2011 Apr;6(4):482-501. doi: 10.1038/nprot.2011.301. Epub 2011 Mar 24. 10.1038/nprot.2011.301 PubMed 21455185 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pDGM3B 21838 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION contains the AAV3B cap genes, AAV2 rep genes, and also adenovirus
helper genes.
ACCESSION .
VERSION .
KEYWORDS pDGM3B
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 21838)
AUTHORS Khan IF, Hirata RK, Russell DW
TITLE AAV-mediated gene targeting methods for human cells.
JOURNAL Nat Protoc. 2011 Apr;6(4):482-501. doi: 10.1038/nprot.2011.301. Epub
2011 Mar 24.
PUBMED 21455185
REFERENCE 2 (bases 1 to 21838)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..21838
/organism="synthetic DNA construct"
/mol_type="other DNA"
repeat_region 21..123
/label=ITR
/note="inverted terminal repeat of human adenovirus
serotype 5"
misc_signal 211..361
/label=Ad5 Psi
/note="packaging signal for adenovirus serotype 5"
primer_bind 483..499
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 483..499
/label=pBluescriptSK
/note="For pBluescript vector"
CDS 5277..5288
/codon_start=1
/product="WELQut protease recognition and cleavage site"
/label=WELQut site
/translation="WELQ"
regulatory 7510..7519
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
repeat_region 19735..19837
/label=ITR
/note="inverted terminal repeat of human adenovirus
serotype 5"
rep_origin complement(20028..20616)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(20108..20127)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(20768..21628)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 21391..21410
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(21629..21733)
/gene="bla"
/label=AmpR promoter
primer_bind 21801..21819
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 ggatccgaat tcttaattaa catcatcaat aatatacctt attttggatt gaagccaata
61 tgataatgag ggggtggagt ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg
121 tagtagtgtg gcggaagtgt gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg
181 tggcaaaagt gacgtttttg gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg
241 gttttaggcg gatgttgtag taaatttggg cgtaaccgag taagatttgg ccattttcgc
301 gggaaaactg aataagagga agtgaaatct gaataatttt gtgttactca tagcgcgtaa
361 tatttgtcta gggccgcggg gactttgacc gtttacgtgg agactcgccc aggtgttttt
421 ctcaggtgtt ttccgcgttc cgggtcaaag ttggcgtttt attattatag tcagggggat
481 cctctagaac tagtggatcc gtccctagaa gtaaaaaagg gaaaaaagag tgtgtttgtc
541 aaaataggag acaggtggtg gcaaccaggg acttataggg gaccttacat ctacagacca
601 acagatgccc ccttaccata tacaggaaga tatgacttaa attgggatag gtgggtcaca
661 atcaacggct ataaagtgtt atacagatcc ctcccctttc gtgaaagact cgccagagct
721 agacctcctt ggtgtatgct aactgagaaa gagaaagacg acatgaaaca acaggtacat
781 gattatattt atctaggaac aggaatgcac ttttggggaa aggttttcca taccaaggaa
841 ggggcagtgg ctggactgat agaacattat tctgcaaaaa cttatggtat gagttattat
901 gattagcctt tatttgccca accttgcggt tcccagggtt taaataagtt tatggttaca
961 aactgttctt aaaacaagga tgtgagacaa gtggtttcct gagttggttt ggtatcaaat
1021 gttctgatct gagctcttag tgttctattt tcctatgttc ttttggaatc tatccaagtc
1081 ttatgtaaat gcttatgtaa accataatat aaaagagtgc tgattttttg agtaaacttg
1141 caacagtcct aacattcttc tctcgtgtgt ttgtgtctgt tcgccatccc gtctccgctc
1201 gtcacttatc cttcactttt cagagggtcc ccccgcagat cccggtcacc ctcaggtcgg
1261 gacctgcaga agacgcccga gtgagcacgc agggtctcca ttttgaagcg ggaggtttga
1321 acgcgcagcc gccatgccgg ggttttacga gattgtgatt aaggtcccca gcgaccttga
1381 cgagcatctg cccggcattt ctgacagctt tgtgaactgg gtggccgaga aggaatggga
1441 gttgccgcca gattctgaca tggatctgaa tctgattgag caggcacccc tgaccgtggc
1501 cgagaagctg cagcgcgact ttctgacgga atggcgccgt gtgagtaagg ccccggaggc
1561 ccttttcttt gtgcaatttg agaagggaga gagctacttc cacatgcacg tgctcgtgga
1621 aaccaccggg gtgaaatcca tggttttggg acgtttcctg agtcagattc gcgaaaaact
1681 gattcagaga atttaccgcg ggatcgagcc gactttgcca aactggttcg cggtcacaaa
1741 gaccagaaat ggcgccggag gcgggaacaa ggtggtggat gagtgctaca tccccaatta
1801 cttgctcccc aaaacccagc ctgagctcca gtgggcgtgg actaatatgg aacagtattt
1861 aagcgcctgt ttgaatctca cggagcgtaa acggttggtg gcgcagcatc tgacgcacgt
1921 gtcgcagacg caggagcaga acaaagagaa tcagaatccc aattctgatg cgccggtgat
1981 cagatcaaaa acttcagcca ggtacatgga gctggtcggg tggctcgtgg acaaggggat
2041 tacctcggag aagcagtgga tccaggagga ccaggcctca tacatctcct tcaatgcggc
2101 ctccaactcg cggtcccaaa tcaaggctgc cttggacaat gcgggaaaga ttatgagcct
2161 gactaaaacc gcccccgact acctggtggg ccagcagccc gtggaggaca tttccagcaa
2221 tcggatttat aaaattttgg aactaaacgg gtacgatccc caatatgcgg cttccgtctt
2281 tctgggatgg gccacgaaaa agttcggcaa gaggaacacc atctggctgt ttgggcctgc
2341 aactaccggg aagaccaaca tcgcggaggc catagcccac actgtgccct tctacgggtg
2401 cgtaaactgg accaatgaga actttccctt caacgactgt gtcgacaaga tggtgatctg
2461 gtgggaggag ggcaagatga cggccaaggt cgtggagagc gccaaggcca ttctgggcgg
2521 aagcaaggtg cgcgtggacc aaaagtgcaa gtcatcggcc cagatcgaac ccactcccgt
2581 gatcgtcacc tccaacacca acatgtgcgc cgtgattgac gggaacagca ccaccttcga
2641 gcatcagcag ccgctgcagg accggatgtt taaatttgaa cttacccgcc gtttggacca
2701 tgactttggg aaggtcacca aacaggaagt aaaggacttt ttccggtggg cttccgatca
2761 cgtgactgac gtggctcatg agttctacgt cagaaagggt ggagctaaga aacgccccgc
2821 ctccaatgac gcggatgtaa gcgagccaaa acggcagtgc acgtcacttg cgcagccgac
2881 aacgtcagac gcggaagcac cggcggacta cgcggacagg taccaaaaca aatgttctcg
2941 tcacgtgggc atgaatctga tgctttttcc ctgtaaaaca tgcgagagaa tgaatcaaat
3001 ttccaatgtc tgttttacgc atggtcaaag agactgtggg gaatgcttcc ctggaatgtc
3061 agaatctcaa cccgtttctg tcgtcaaaaa gaagacttat cagaaactgt gtccaattca
3121 tcatatcctg ggaagggcac ccgagattgc ctgttcggcc tgcgatttgg ccaatgtgga
3181 cttggatgac tgtgtttctg agcaataaat gacttaaacc aggtatggct gctgacggtt
3241 atcttccaga ttggctcgag gacaaccttt ctgaaggcat tcgtgagtgg tgggctctga
3301 aacctggagt ccctcaaccc aaagcgaacc aacaacacca ggacaaccgt cggggtcttg
3361 tgcttccggg ttacaaatac ctcggacccg gtaacggact cgacaaagga gagccggtca
3421 acgaggcgga cgcggcagcc ctcgaacacg acaaagctta cgaccagcag ctcaaggccg
3481 gtgacaaccc gtacctcaag tacaaccacg ccgacgccga gtttcaggag cgtcttcaag
3541 aagatacgtc ttttgggggc aaccttggca gagcagtctt ccaggccaaa aagaggatcc
3601 ttgagcctct tggtctggtt gaggaagcag ctaaaacggc tcctggaaag aagaggcctg
3661 tagatcagtc tcctcaggaa ccggactcat catctggtgt tggcaaatcg ggcaaacagc
3721 ctgccagaaa aagactaaat ttcggtcaga ctggcgactc agagtcagtc ccagaccctc
3781 aacctctcgg agaaccacca gcagccccca caagtttggg atctaataca atggcttcag
3841 gcggtggcgc accaatggca gacaataacg agggtgccga tggagtgggt aattcctcag
3901 gaaattggca ttgcgattcc caatggctgg gcgacagagt catcaccacc agcaccagaa
3961 cctgggccct gcccacttac aacaaccatc tctacaagca aatctccagc caatcaggag
4021 cttcaaacga caaccactac tttggctaca gcaccccttg ggggtatttt gactttaaca
4081 gattccactg ccacttctca ccacgtgact ggcagcgact cattaacaac aactggggat
4141 tccggcccaa gaaactcagc ttcaagctct tcaacatcca agttaaagag gtcacgcaga
4201 acgatggcac gacgactatt gccaataacc ttaccagcac ggttcaagtg tttacggact
4261 cggagtatca gctcccgtac gtgctcgggt cggcgcacca aggctgtctc ccgccgtttc
4321 cagcggacgt cttcatggtc cctcagtatg gatacctcac cctgaacaac ggaagtcaag
4381 cggtgggacg ctcatccttt tactgcctgg agtacttccc ttcgcagatg ctaaggactg
4441 gaaataactt ccaattcagc tataccttcg aggatgtacc ttttcacagc agctacgctc
4501 acagccagag tttggatcgc ttgatgaatc ctcttattga tcagtatctg tactacctga
4561 acagaacgca aggaacaacc tctggaacaa ccaaccaatc acggctgctt tttagccagg
4621 ctgggcctca gtctatgtct ttgcaggcca gaaattggct acctgggccc tgctaccggc
4681 aacagagact ttcaaagact gctaacgaca acaacaacag taactttcct tggacagcgg
4741 ccagcaaata tcatctcaat ggccgcgact cgctggtgaa tccaggacca gctatggcca
4801 gtcacaagga cgatgaagaa aaatttttcc ctatgcacgg caatctaata tttggcaaag
4861 aagggacaac ggcaagtaac gcagaattag ataatgtaat gattacggat gaagaagaga
4921 ttcgtaccac caatcctgtg gcaacagagc agtatggaac tgtggcaaat aacttgcaga
4981 gctcaaatac agctcccacg actagaactg tcaatgatca gggggcctta cctggcatgg
5041 tgtggcaaga tcgtgacgtg taccttcaag gacctatctg ggcaaagatt cctcacacgg
5101 atggacactt tcatccttct cctctgatgg gaggctttgg actgaaacat ccgcctcctc
5161 aaatcatgat caaaaatact ccggtaccgg caaatcctcc gacgactttc agcccggcca
5221 agtttgcttc atttatcact cagtactcca ctggacaggt cagcgtggaa attgagtggg
5281 agctacagaa agaaaacagc aaacgttgga atccagagat tcagtacact tccaactaca
5341 acaagtctgt taatgtggac tttactgtag acactaatgg tgtttatagt gaacctcgcc
5401 ctattggaac ccggtatctc acacgaaact tgtaatcctg gttaatcaat aaaccgttta
5461 attcgtttca gttgaacttt ggctcttgtg cacttcttat cttatcttgt ttccatggct
5521 atcgatgcat gtccttgggt ccggcctgct gaatgcgcag gcggtcggcc atgccccagg
5581 cttcgttttg acatcggcgc aggtctttgt agtagtcttg catgagcctt tctaccggca
5641 cttcttcttc tccttcctct tgtcctgcat ctcttgcatc tatcgctgcg gcggcggcgg
5701 agtttggccg taggtggcgc cctcttcctc ccatcgtgtg accccgaagc ccctcatcgg
5761 ctgaagcagg gctaggtcgg cgacaacgcg ctcggctaat atggcctgct gcacctgcgt
5821 gagggtagac tggaagtcat ccatgtccac aaagcggtgg tatgcgcccg tgttgatggt
5881 gtaagtgcag ttggccataa cggaccagtt aacggtctgg tgacccggct gcgagagctc
5941 ggtgtacctg agacgcgagt aagccctcga gtcaaatacg tagtcgttac aagtccgcac
6001 caggtactgg tatcccacca aaaagtgcgg cggcggctgg cggtagaggg gccagcgtag
6061 ggtggccggg gctccggggg cgagatcttc caacataagg cgatgatatc cgtagatgta
6121 cctggacatc caggtgatgc cggcggcggt ggtggaggcg cgcggaaagt cgcggacgcg
6181 gttccagatg ttgcgcagcg gcaaaaagtg ctccatggtc gggacgctct ggccggtcag
6241 gcgcgcgcaa tcgttgacgc tctagaccgt gcaaaaggag agcctgtaag cgggcactct
6301 tccgtggtct ggtggataaa ttcgcaaggg tatcatggcg gacgaccggg gttcgagccc
6361 cgtatccggc cgtccgccgt gatccatgcg gttaccgccc gcgtgtcgaa cccaggtgtg
6421 cgacgtcaga caacggggga gtgctccttt tggcttcctt ccaggcgcgg cggctgctgc
6481 gctagctttt ttggccactg gccgcgcgca gcgtaagcgg ttaggctgga aagcgaaagc
6541 attaagtggc tcgctccctg tagccggagg gttattttcc aagggttgag tcgcgggacc
6601 cccggttcga gtctcggacc ggccggactg cggcgaacgg gggtttgtct ccccgtcatg
6661 caagaccccg cttgcaaatt cctccggaaa cagggacgag cccctttttt gcttttccca
6721 gatgcatccg gtgctgcggc agatgcgccc ccctcctcag cagcggcaag agcaagagca
6781 gcggcagaca tgcagggcac cctcccctcc tcctaccgcg tcaggagggg cgacatccgc
6841 ggttgacgcg gcagcagatg gtgattacga acccccgcgg cgccgggccc ggcactacct
6901 ggacttggag gagggcgagg gcctggcgcg gctaggagcg ccctctcctg agcggcaccc
6961 aagggtgcag ctgaagcgtg atacgcgtga ggcgtacgtg ccgcggcaga acctgtttcg
7021 cgaccgcgag ggagaggagc ccgaggagat gcgggatcga aagttccacg cagggcgcga
7081 gctgcggcat ggcctgaatc gcgagcggtt gctgcgcgag gaggactttg agcccgacgc
7141 gcgaaccggg attagtcccg cgcgcgcaca cgtggcggcc gccgacctgg taaccgcata
7201 cgagcagacg gtgaaccagg gcgatcgcac cctttggcgc atcccattct ccagtaactt
7261 tatgtccatg ggcgcactca cagacctggg ccaaaacctt ctctacgcca actccgccca
7321 cgcgctagac atgacttttg aggtggatcc catggacgag cccacccttc tttatgtttt
7381 gtttgaagtc tttgacgtgg tccgtgtgca ccagccgcac cgcggcgtca tcgaaaccgt
7441 gtacctgcgc acgcccttct cggccggcaa cgccacaaca taaagaagca agcaacatca
7501 acaacagctg ccgccatggg ctccagtgag caggaactga aagccattgt caaagatctt
7561 ggttgtgggc catatttttt gggcacctat gacaagcgct ttccaggctt tgtttctcca
7621 cacaagctcg cctgcgccat agtcaatacg gccggtcgcg agactggggg cgtacactgg
7681 atggcctttg cctggaaccc gcactcaaaa acatgctacc tctttgagcc ctttggcttt
7741 tctgaccagc gactcaagca ggtttaccag tttgagtacg agtcactcct gcgccgtagc
7801 gccattgctt cttcccccga ccgctgtata acgctggaaa agtccaccca aagcgtacag
7861 gggcccaact cggccgcctg tggactattc tgctgcatgt ttctccacgc ctttgccaac
7921 tggccccaaa ctcccatgga tcacaacccc accatgaacc ttattaccgg ggtacccaac
7981 tccatgctca acagtcccca ggtacagccc accctgcgtc gcaaccagga acagctctac
8041 agcttcctgg agcgccactc gccctacttc cgcagccaca gtgcgcagat taggagcgcc
8101 acttcttttt gtcacttgaa aaacatgtaa aaataatgta ctagagacac tttcaataaa
8161 ggcaaatgct tttatttgta cactctcggg tgattattta cccccaccct tgccgtctgc
8221 gccgtttaaa aatcaaaggg gttctgccgc gcatcgctat gcgccactgg cagggacacg
8281 ttgcgatact ggtgtttagt gctccactta aactcaggca caaccatccg cggcagctcg
8341 gtgaagtttt cactccacag gctgcgcacc atcaccaacg cgtttagcag gtcgggcgcc
8401 gatatcttga agtcgcagtt ggggcctccg ccctgcgcgc gcgagttgcg atacacaggg
8461 ttgcagcact ggaacactat cagcgccggg tggtgcacgc tggccagcac gctcttgtcg
8521 gagatcagat ccgcgtccag gtcctccgcg ttgctcaggg cgaacggagt caactttggt
8581 agctgccttc ccaaaaaggg cgcgtgccca ggctttgagt tgcactcgca ccgtagtggc
8641 atcaaaaggt gaccgtgccc ggtctgggcg ttaggataca gcgcctgcat aaaagccttg
8701 atctgcttaa aagccacctg agcctttgcg ccttcagaga agaacatgcc gcaagacttg
8761 ccggaaaact gattggccgg acaggccgcg tcgtgcacgc agcaccttgc gtcggtgttg
8821 gagatctgca ccacatttcg gccccaccgg ttcttcacga tcttggcctt gctagactgc
8881 tccttcagcg cgcgctgccc gttttcgctc gtcacatcca tttcaatcac gtgctcctta
8941 tttatcataa tgcttccgtg tagacactta agctcgcctt cgatctcagc gcagcggtgc
9001 agccacaacg cgcagcccgt gggctcgtga tgcttgtagg tcacctctgc aaacgactgc
9061 aggtacgcct gcaggaatcg ccccatcatc gtcacaaagg tcttgttgct ggtgaaggtc
9121 agctgcaacc cgcggtgctc ctcgttcagc caggtcttgc atacggccgc cagagcttcc
9181 acttggtcag gcagtagttt gaagttcgcc tttagatcgt tatccacgtg gtacttgtcc
9241 atcagcgcgc gcgcagcctc catgcccttc tcccacgcag acacgatcgg cacactcagc
9301 gggttcatca ccgtaatttc actttccgct tcgctgggct cttcctcttc ctcttgcgtc
9361 cgcataccac gcgccactgg gtcgtcttca ttcagccgcc gcactgtgcg cttacctcct
9421 ttgccatgct tgattagcac cggtgggttg ctgaaaccca ccatttgtag cgccacatct
9481 tctctttctt cctcgctgtc cacgattacc tctggtgatg gcgggcgctc gggcttggga
9541 gaagggcgct tctttttctt cttgggcgca atggccaaat ccgccgccga ggtcgatggc
9601 cgcgggctgg gtgtgcgcgg caccagcgcg tcttgtgatg agtcttcctc gtcctcggac
9661 tcgatacgcc gcctcatccg cttttttggg ggcgcccggg gaggcggcgg cgacggggac
9721 ggggacgaca cgtcctccat ggttggggga cgtcgcgccg caccgcgtcc gcgctcgggg
9781 gtggtttcgc gctgctcctc ttcccgactg gccatttcct tctcctatag gcagaaaaag
9841 atcatggagt cagtcgagaa gaaggacagc ctaaccgccc cctctgagtt cgccaccacc
9901 gcctccaccg atgccgccaa cgcgcctacc accttccccg tcgaggcacc cccgcttgag
9961 gaggaggaag tgattatcga gcaggaccca ggttttgtaa gcgaagacga cgaggaccgc
10021 tcagtaccaa cagaggataa aaagcaagac caggacaacg cagaggcaaa cgaggaacaa
10081 gtcgggcggg gggacgaaag gcatggcgac tacctagatg tgggagacga cgtgctgttg
10141 aagcatctgc agcgccagtg cgccattatc tgcgacgcgt tgcaagagcg cagcgatgtg
10201 cccctcgcca tagcggatgt cagccttgcc tacgaacgcc acctattctc accgcgcgta
10261 ccccccaaac gccaagaaaa cggcacatgc gagcccaacc cgcgcctcaa cttctacccc
10321 gtatttgccg tgccagaggt gcttgccacc tatcacatct ttttccaaaa ctgcaagata
10381 cccctatcct gccgtgccaa ccgcagccga gcggacaagc agctggcctt gcggcagggc
10441 gctgtcatac ctgatatcgc ctcgctcaac gaagtgccaa aaatctttga gggtcttgga
10501 cgcgacgaga agcgcgcggc aaacgctctg caacaggaaa acagcgaaaa tgaaagtcac
10561 tctggagtgt tggtggaact cgagggtgac aacgcgcgcc tagccgtact aaaacgcagc
10621 atcgaggtca cccactttgc ctacccggca cttaacctac cccccaaggt catgagcaca
10681 gtcatgagtg agctgatcgt gcgccgtgcg cagcccctgg agagggatgc aaatttgcaa
10741 gaacaaacag aggagggcct acccgcagtt ggcgacgagc agctagcgcg ctggcttcaa
10801 acgcgcgagc ctgccgactt ggaggagcga cgcaaactaa tgatggccgc agtgctcgtt
10861 accgtggagc ttgagtgcat gcagcggttc tttgctgacc cggagatgca gcgcaagcta
10921 gaggaaacat tgcactacac ctttcgacag ggctacgtac gccaggcctg caagatctcc
10981 aacgtggagc tctgcaacct ggtctcctac cttggaattt tgcacgaaaa ccgccttggg
11041 caaaacgtgc ttcattccac gctcaagggc gaggcgcgcc gcgactacgt ccgcgactgc
11101 gtttacttat ttctatgcta cacctggcag acggccatgg gcgtttggca gcagtgcttg
11161 gaggagtgca acctcaagga gctgcagaaa ctgctaaagc aaaacttgaa ggacctatgg
11221 acggccttca acgagcgctc cgtggccgcg cacctggcgg acatcatttt ccccgaacgc
11281 ctgcttaaaa ccctgcaaca gggtctgcca gacttcacca gtcaaagcat gttgcagaac
11341 tttaggaact ttatcctaga gcgctcagga atcttgcccg ccacctgctg tgcacttcct
11401 agcgactttg tgcccattaa gtaccgcgaa tgccctccgc cgctttgggg ccactgctac
11461 cttctgcagc tagccaacta ccttgcctac cactctgaca taatggaaga cgtgagcggt
11521 gacggtctac tggagtgtca ctgtcgctgc aacctatgca ccccgcaccg ctccctggtt
11581 tgcaattcgc agctgcttaa cgaaagtcaa attatcggta cctttgagct gcagggtccc
11641 tcgcctgacg aaaagtccgc ggctccgggg ttgaaactca ctccggggct gtggacgtcg
11701 gcttaccttc gcaaatttgt acctgaggac taccacgccc acgagattag gttctacgaa
11761 gaccaatccc gcccgcctaa tgcggagctt accgcctgcg tcattaccca gggccacatt
11821 cttggccaat tgcaagccat caacaaagcc cgccaagagt ttctgctacg aaagggacgg
11881 ggggtttact tggaccccca gtccggcgag gagctcaacc caatcccccc gccgccgcag
11941 ccctatcagc agcagccgcg ggcccttgct tcccaggatg gcacccaaaa agaagctgca
12001 gctgccgccg ccacccacgg acgaggagga atactgggac agtcaggcag aggaggtttt
12061 ggacgaggag gaggaggaca tgatggaaga ctgggagagc ctagacgagg aagcttccga
12121 ggtcgaagag gtgtcagacg aaacaccgtc accctcggtc gcattcccct cgccggcgcc
12181 ccagaaatcg gcaaccggtt ccagcatggc tacaacctcc gctcctcagg cgccgccggc
12241 actgcccgtt cgccgaccca accgtagatg ggacaccact ggaaccaggg ccggtaagtc
12301 caagcagccg ccgccgttag cccaagagca acaacagcgc caaggctacc gctcatggcg
12361 cgggcacaag aacgccatag ttgcttgctt gcaagactgt gggggcaaca tctccttcgc
12421 ccgccgcttt cttctctacc atcacggcgt ggccttcccc cgtaacatcc tgcattacta
12481 ccgtcatctc tacagcccat actgcaccgg cggcagcggc agcaacagca gcggccacac
12541 agaagcaaag gcgaccggat agcaagactc tgacaaagcc caagaaatcc acagcggcgg
12601 cagcagcagg aggaggagcg ctgcgtctgg cgcccaacga acccgtatcg acccgcgagc
12661 ttagaaacag gatttttccc actctgtatg ctatatttca acagagcagg ggccaagaac
12721 aagagctgaa aataaaaaac aggtctctgc gatccctcac ccgcagctgc ctgtatcaca
12781 aaagcgaaga tcagcttcgg cgcacgctgg aagacgcgga ggctctcttc agtaaatact
12841 gcgcgctgac tcttaaggac tagtttcgcg ccctttctca aatttaagcg cgaaaactac
12901 gtcatctcca gcggccacac ccggcgccag cacctgttgt cagcgccatt atgagcaagg
12961 aaattcccac gccctacatg tggagttacc agccacaaat gggacttgcg gctggagctg
13021 cccaagacta ctcaacccga ataaactaca tgagcgcggg accccacatg atatcccggg
13081 tcaacggaat acgcgcccac cgaaaccgaa ttctcctgga acaggcggct attaccacca
13141 cacctcgtaa taaccttaat ccccgtagtt ggcccgctgc cctggtgtac caggaaagtc
13201 ctgctcccac cactgtggta cttcccagag acgcccaggc cgaagttcag atgactaact
13261 caggggcgca gcttgcgggc ggctttcgtc acagggtgcg gtcgcccggg cagggtataa
13321 ctcacctgac aatcagaggg cgaggtattc agctcaacga cgagtcggtg agctcctcgc
13381 ttggtctccg tccggacggg acatttcaga tcggcggcgc cggccgctct tcattcacgc
13441 ctcgtcaggc aatcctaact ctgcagacct cgtcctctga gccgcgctct ggaggcattg
13501 gaactctgca atttattgag gagtttgtgc catcggtcta ctttaacccc ttctcgggac
13561 ctcccggcca ctatccggat caatttattc ctaactttga cgcggtaaag gactcggcgg
13621 acggctacga ctgaatgtta agtggagagg cagagcaact gcgcctgaaa cacctggtcc
13681 actgtcgccg ccacaagtgc tttgcccgcg actccggtga gttttgctac tttgaattgc
13741 ccgaggatca tatcgagggc ccggcgcacg gcgtccggct taccgcccag ggagagcttg
13801 cccgtagcct gattcgggag tttacccagc gccccctgct agttgagcgg gacaggggac
13861 cctgtgttct cactgtgatt tgcaactgtc ctaaccctgg attacatcaa gatctttgtt
13921 gccatctctg tgctgagtat aataaataca gaaattaaaa tatactgggg ctcctatcgc
13981 catcctgtaa acgccaccgt cttcacccgc ccaagcaaac caaggcgaac cttacctggt
14041 acttttaaca tctctccctc tgtgatttac aacagtttca acccagacgg agtgagtcta
14101 cgagagaacc tctccgagct cagctactcc atcagaaaaa acaccaccct ccttacctgc
14161 cgggaacgta cgagtgcgtc accggccgct gcaccacacc taccgcctga ccgtaaacca
14221 gactttttcc ggacagacct caataactct gtttaccaga acaggaggtg agcttagaaa
14281 acccttaggg tattaggcca aaggcgcagc tactgtgggg tttatgaaca attcaagcaa
14341 ctctacgggc tattctaatt caggtttctc tagaaatgga cggaattatt acagagcagc
14401 gcctgctaga aagacgcagg gcagcggccg agcaacagcg catgaatcaa gagctccaag
14461 acatggttaa cttgcaccag tgcaaaaggg gtatcttttg tctggtaaag caggccaaag
14521 tcacctacga cagtaatacc accggacacc gccttagcta caagttgcca accaagcgtc
14581 agaaattggt ggtcatggtg ggagaaaagc ccattaccat aactcagcac tcggtagaaa
14641 ccgaaggctg cattcactca ccttgtcaag gacctgagga tctctgcacc cttattaaga
14701 ccctgtgcgg tctcaaagat cttattccct ttaactaata aaaaaaaata ataaagcatc
14761 acttacttaa aatcagttag caaatttctg tccagtttat tcagcagcac ctccttgccc
14821 tcctcccagc tctggtattg cagcttcctc ctggctgcaa actttctcca caatctaaat
14881 ggaatgtcag tttcctcctg ttcctgtcca tccgcaccca ctatcttcat gttgttgcag
14941 atgaagcgcg caagaccgtc tgaagatacc ttcaaccccg tgtatccata tgacacggaa
15001 accggtcctc caactgtgcc ttttcttact cctccctttg tatcccccaa tgggtttcaa
15061 gagagtcccc ctggggtact ctctttgcgc ctatccgaac ctctagttac ctccaatggc
15121 atgcttgcgc tcaaaatggg caacggcctc tctctggacg aggccggcaa ccttacctcc
15181 caaaatgtaa ccactgtgag cccacctctc aaaaaaacca agtcaaacat aaacctggaa
15241 atatctgcac ccctcacagt tacctcagaa gccctaactg tggctgccgc cgcacctcta
15301 atggtcgcgg gcaacacact caccatgcaa tcacaggccc cgctaaccgt gcacgactcc
15361 aaacttagca ttgccaccca aggacccctc acagtgtcag aaggaaagct agccctgcaa
15421 acatcaggcc ccctcaccac caccgatagc agtaccctta ctatcactgc ctcaccccct
15481 ctaactactg ccactggtag cttgggcatt gacttgaaag agcccattta tacacaaaat
15541 ggaaaactag gactaaagta cggggctcct ttgcatgtaa cagacgacct aaacactttg
15601 accgtagcaa ctggtccagg tgtgactatt aataatactt ccttgcaaac taaagttact
15661 ggagccttgg gttttgattc acaaggcaat atgcaactta atgtagcagg aggactaagg
15721 attgattctc aaaacagacg ccttatactt gatgttagtt atccgtttga tgctcaaaac
15781 caactaaatc taagactagg acagggccct ctttttataa actcagccca caacttggat
15841 attaactaca acaaaggcct ttacttgttt acagcttcaa acaattccaa aaagcttgag
15901 gttaacctaa gcactgccaa ggggttgatg tttgacgcta cagccatagc cattaatgca
15961 ggagatgggc ttgaatttgg ttcacctaat gcaccaaaca caaatcccct caaaacaaaa
16021 attggccatg gcctagaatt tgattcaaac aaggctatgg ttcctaaact aggaactggc
16081 cttagttttg acagcacagg tgccattaca gtaggaaaca aaaataatga taagctaact
16141 ttgtggacca caccagctcc atctcctaac tgtagactaa atgcagagaa agatgctaaa
16201 ctcactttgg tcttaacaaa atgtggcagt caaatacttg ctacagtttc agttttggct
16261 gttaaaggca gtttggctcc aatatctgga acagttcaaa gtgctcatct tattataaga
16321 tttgacgaaa atggagtgct actaaacaat tccttcctgg acccagaata ttggaacttt
16381 agaaatggag atcttactga aggcacagcc tatacaaacg ctgttggatt tatgcctaac
16441 ctatcagctt atccaaaatc tcacggtaaa actgccaaaa gtaacattgt cagtcaagtt
16501 tacttaaacg gagacaaaac taaacctgta acactaacca ttacactaaa cggtacacag
16561 gaaacaggag acacaactcc aagtgcatac tctatgtcat tttcatggga ctggtctggc
16621 cacaactaca ttaatgaaat atttgccaca tcctcttaca ctttttcata cattgcccaa
16681 gaataaagaa tcgtttgtgt tatgtttcaa cgtgtttatt tttcaattgc agaaaatttc
16741 aagtcatttt tcattcagta gtatagcccc accaccacat agcttataca gatcaccgta
16801 ccttaatcaa actcacagaa ccctagtatt caacctgcca cctccctccc aacacacaga
16861 gtacacagtc ctttctcccc ggctggcctt aaaaagcatc atatcatggg taacagacat
16921 attcttaggt gttatattcc acacggtttc ctgtcgagcc aaacgctcat cagtgatatt
16981 aataaactcc ccgggcagct cacttaagtt catgtcgctg tccagctgct gagccacagg
17041 ctgctgtcca acttgcggtt gcttaacggg cggcgaagga gaagtccacg cctacatggg
17101 ggtagagtca taatcgtgca tcaggatagg gcggtggtgc tgcagcagcg cgcgaataaa
17161 ctgctgccgc cgccgctccg tcctgcagga atacaacatg gcagtggtct cctcagcgat
17221 gattcgcacc gcccgcagca taaggcgcct tgtcctccgg gcacagcagc gcaccctgat
17281 ctcacttaaa tcagcacagt aactgcagca cagcaccaca atattgttca aaatcccaca
17341 gtgcaaggcg ctgtatccaa agctcatggc ggggaccaca gaacccacgt ggccatcata
17401 ccacaagcgc aggtagatta agtggcgacc cctcataaac acgctggaca taaacattac
17461 ctcttttggc atgttgtaat tcaccacctc ccggtaccat ataaacctct gattaaacat
17521 ggcgccatcc accaccatcc taaaccagct ggccaaaacc tgcccgccgg ctatacactg
17581 cagggaaccg ggactggaac aatgacagtg gagagcccag gactcgtaac catggatcat
17641 catgctcgtc atgatatcaa tgttggcaca acacaggcac acgtgcatac acttcctcag
17701 gattacaagc tcctcccgcg ttagaaccat atcccaggga acaacccatt cctgaatcag
17761 cgtaaatccc acactgcagg gaagacctcg cacgtaactc acgttgtgca ttgtcaaagt
17821 gttacattcg ggcagcagcg gatgatcctc cagtatggta gcgcgggttt ctgtctcaaa
17881 aggaggtaga cgatccctac tgtacggagt gcgccgagac aaccgagatc gtgttggtcg
17941 tagtgtcatg ccaaatggaa cgccggacgt agtcatattt cctgaagcaa aaccaggtgc
18001 gggcgtgaca aacagatctg cgtctccggt ctcgccgctt agatcgctct gtgtagtagt
18061 tgtagtatat ccactctctc aaagcatcca ggcgccccct ggcttcgggt tctatgtaaa
18121 ctccttcatg cgccgctgcc ctgataacat ccaccaccgc agaataagcc acacccagcc
18181 aacctacaca ttcgttctgc gagtcacaca cgggaggagc gggaagagct ggaagaacca
18241 tgtttttttt tttattccaa aagattatcc aaaacctcaa aatgaagatc tattaagtga
18301 acgcgctccc ctccggtggc gtggtcaaac tctacagcca aagaacagat aatggcattt
18361 gtaagatgtt gcacaatggc ttccaaaagg caaacggccc tcacgtccaa gtggacgtaa
18421 aggctaaacc cttcagggtg aatctcctct ataaacattc cagcaccttc aaccatgccc
18481 aaataattct catctcgcca ccttctcaat atatctctaa gcaaatcccg aatattaagt
18541 ccggccattg taaaaatctg ctccagagcg ccctccacct tcagcctcaa gcagcgaatc
18601 atgattgcaa aaattcaggt tcctcacaga cctgtataag attcaaaagc ggaacattaa
18661 caaaaatacc gcgatcccgt aggtcccttc gcagggccag ctgaacataa tcgtgcaggt
18721 ctgcacggac cagcgcggcc acttccccgc caggaaccat gacaaaagaa cccacactga
18781 ttatgacacg catactcgga gctatgctaa ccagcgtagc cccgatgtaa gcttgttgca
18841 tgggcggcga tataaaatgc aaggtgctgc tcaaaaaatc aggcaaagcc tcgcgcaaaa
18901 aagaaagcac atcgtagtca tgctcatgca gataaaggca ggtaagctcc ggaaccacca
18961 cagaaaaaga caccattttt ctctcaaaca tgtctgcggg tttctgcata aacacaaaat
19021 aaaataacaa aaaaacattt aaacattaga agcctgtctt acaacaggaa aaacaaccct
19081 tataagcata agacggacta cggccatgcc ggcgtgaccg taaaaaaact ggtcaccgtg
19141 attaaaaagc accaccgaca gctcctcggt catgtccgga gtcataatgt aagactcggt
19201 aaacacatca ggttgattca catcggtcag tgctaaaaag cgaccgaaat agcccggggg
19261 aatacatacc cgcaggcgta gagacaacat tacagccccc ataggaggta taacaaaatt
19321 aataggagag aaaaacacat aaacacctga aaaaccctcc tgcctaggca aaatagcacc
19381 ctcccgctcc agaacaacat acagcgcttc cacagcggca gccataacag tcagccttac
19441 cagtaaaaaa gaaaacctat taaaaaaaca ccactcgaca cggcaccagc tcaatcagtc
19501 acagtgtaaa aaagggccaa gtgcagagcg agtatatata ggactaaaaa atgacgtaac
19561 ggttaaagtc cacaaaaaac acccagaaaa ccgcacgcga acctacgccc agaaacgaaa
19621 gccaaaaaac ccacaacttc ctcaaatcgt cacttccgtt ttcccacgtt acgtcacttc
19681 ccattttaag aaaactacaa ttcccaacac atacaagtta ctccgcccta aaacctacgt
19741 cacccgcccc gttcccacgc cccgcgccac gtcacaaact ccaccccctc attatcatat
19801 tggcttcaat ccaaaataag gtatattatt gatgatgtta attaagaatt cggatctgcg
19861 acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg
19921 cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa
19981 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
20041 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
20101 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
20161 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
20221 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
20281 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
20341 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
20401 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
20461 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
20521 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
20581 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
20641 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
20701 aaaaggatct tcacctagat ccttttaaat caatctaaag tatatatgag taaacttggt
20761 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
20821 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
20881 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
20941 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
21001 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
21061 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
21121 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
21181 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
21241 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
21301 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
21361 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
21421 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
21481 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
21541 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
21601 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
21661 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
21721 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
21781 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaatt
//
