| 质粒名称: | pCDH-3xFLAG-TERT |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 51631 |
| 存储实验室: | Steven Artandi |
|
载体骨架基本信息 |
|
| 载体名称: | pCDH |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | 科扎克后的N端3xFLAG标签 |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | TERT |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 3399 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | 3xFLAG（主干上的N终端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AscI(未破坏) |
| 3' 克隆位点: | PacI(未破坏) |
| 5' 测序引物 | CMV |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pCDH-3xFLAG-TERT was a gift from Steven Artandi (Addgene plasmid # 51631 ; http://n2t.net/addgene:51631 ; RRID:Addgene_51631) |
| 参考文献部分: | TPP1 OB-fold domain controls telomere maintenance by recruiting telomerase to chromosome ends. Zhong FL, Batista LF, Freund A, Pech MF, Venteicher AS, Artandi SE. Cell. 2012 Aug 3;150(3):481-94. doi: 10.1016/j.cell.2012.07.012. 10.1016/j.cell.2012.07.012 PubMed 22863003 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCDH-3xFLAG-TERT 11084 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Mammalian expression of 3xFLAG tagged TERT, N-terminal..
ACCESSION .
VERSION .
KEYWORDS pCDH-3xFLAG-TERT
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11084)
AUTHORS Zhong FL, Batista LF, Freund A, Pech MF, Venteicher AS, Artandi SE
TITLE TPP1 OB-fold domain controls telomere maintenance by recruiting
telomerase to chromosome ends.
JOURNAL Cell. 2012 Aug 3;150(3):481-94. doi: 10.1016/j.cell.2012.07.012.
PUBMED 22863003
REFERENCE 2 (bases 1 to 11084)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11084
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..204
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 155..175
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 201..225
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 305..370
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
CDS 380..3775
/codon_start=1
/gene="TERT"
/product="human telomerase reverse transcriptase, the
catalytic subunit of telomerase"
/label=hTERT
/note="prevents telomere shortening and allows
postsenescent cells to proliferate beyond crisis (Counter
et al., 1998); also known as EST2"
/translation="PRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPAA
FRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFALLD
GARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFVLVAP
SCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGARR
RGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEEA
TSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYSSGDKEQL
RPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGNH
AQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSPW
QVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRDCA
WLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTETTFQKNRLFFYR
KSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVNM
DYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFVL
RVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHGH
VRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNEASSGLFDVFLRFM
CHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLRLVDDFLL
VTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPWC
GLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRRKLFGVLRLKCHSLFLDLQ
VNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTASLCYSILKA
KNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKLP
GTTLTALEAAANPALPSDFKTILD"
promoter 3830..4041
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 4054..4322
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
CDS 4350..5144
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLAATGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4404..4423)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 5014..5033
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
misc_feature 5151..5739
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5204..5224)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5622..5633)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 5813..6046
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 6118..6239
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6155..6174)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6209..6228
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 6279..6414
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6341..6360
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(6447..6463)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6447..6463)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6460..6482)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6471..6487
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6495..6525)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6540..6561
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6678..6695)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6849..7437)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6929..6948)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7608..8468)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8231..8250
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8469..8573)
/gene="bla"
/label=AmpR promoter
primer_bind 8641..8659
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(8697..8719)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 8819..8838
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 9032..9054
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 9046..9063
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 9047..9063
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 9078..9304
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 9305..9485
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 9532..9657
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 10150..10383
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 10568..10612
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 10879..10995
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
ORIGIN
1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
181 tgggaggttt atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
241 ccacgctgtt ttgacctcca tagaagattc tagagctagc gaattcacca tggggtcccg
301 ggctgactac aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga
361 cgatgacaag gggcgcgccc cgcgcgctcc ccgctgccga gccgtgcgct ccctgctgcg
421 cagccactac cgcgaggtgc tgccgctggc cacgttcgtg cggcgcctgg ggccccaggg
481 ctggcggctg gtgcagcgcg gggacccggc ggctttccgc gcgctggtgg cccagtgcct
541 ggtgtgcgtg ccctgggacg cacggccgcc ccccgccgcc ccctccttcc gccaggtgtc
601 ctgcctgaag gagctggtgg cccgagtgct gcagaggctg tgcgagcgcg gcgcgaagaa
661 cgtgctggcc ttcggcttcg cgctgctgga cggggcccgc gggggccccc ccgaggcctt
721 caccaccagc gtgcgcagct acctgcccaa cacggtgacc gacgcactgc gggggagcgg
781 ggcgtggggg ctgctgctgc gccgcgtggg cgacgacgtg ctggttcacc tgctggcacg
841 ctgcgcgctc tttgtgctgg tggctcccag ctgcgcctac caggtgtgcg ggccgccgct
901 gtaccagctc ggcgctgcca ctcaggcccg gcccccgcca cacgctagtg gaccccgaag
961 gcgtctggga tgcgaacggg cctggaacca tagcgtcagg gaggccgggg tccccctggg
1021 cctgccagcc ccgggtgcga ggaggcgcgg gggcagtgcc agccgaagtc tgccgttgcc
1081 caagaggccc aggcgtggcg ctgcccctga gccggagcgg acgcccgttg ggcaggggtc
1141 ctgggcccac ccgggcagga cgcgtggacc gagtgaccgt ggtttctgtg tggtgtcacc
1201 tgccagaccc gccgaagaag ccacctcttt ggagggtgcg ctctctggca cgcgccactc
1261 ccacccatcc gtgggccgcc agcaccacgc gggcccccca tccacatcgc ggccaccacg
1321 tccctgggac acgccttgtc ccccggtgta cgccgagacc aagcacttcc tctactcctc
1381 aggcgacaag gagcagctgc ggccctcctt cctactcagc tctctgaggc ccagcctgac
1441 tggcgctcgg aggctcgtgg agaccatctt tctgggttcc aggccctgga tgccagggac
1501 tccccgcagg ttgccccgcc tgccccagcg ctactggcaa atgcggcccc tgtttctgga
1561 gctgcttggg aaccacgcgc agtgccccta cggggtgctc ctcaagacgc actgcccgct
1621 gcgagctgcg gtcaccccag cagccggtgt ctgtgcccgg gagaagcccc agggctctgt
1681 ggcggccccc gaggaggagg acacagaccc ccgtcgcctg gtgcagctgc tccgccagca
1741 cagcagcccc tggcaggtgt acggcttcgt gcgggcctgc ctgcgccggc tggtgccccc
1801 aggcctctgg ggctccaggc acaacgaacg ccgcttcctc aggaacacca agaagttcat
1861 ctccctgggg aagcatgcca agctctcgct gcaggagctg acgtggaaga tgagcgtgcg
1921 ggactgcgct tggctgcgca ggagcccagg ggttggctgt gttccggccg cagagcaccg
1981 tctgcgtgag gagatcctgg ccaagttcct gcactggctg atgagtgtgt acgtcgtcga
2041 gctgctcagg tctttctttt atgtcacgga gaccacgttt caaaagaaca ggctcttttt
2101 ctaccggaag agtgtctgga gcaagttgca aagcattgga atcagacagc acttgaagag
2161 ggtgcagctg cgggagctgt cggaagcaga ggtcaggcag catcgggaag ccaggcccgc
2221 cctgctgacg tccagactcc gcttcatccc caagcctgac gggctgcggc cgattgtgaa
2281 catggactac gtcgtgggag ccagaacgtt ccgcagagaa aagagggccg agcgtctcac
2341 ctcgagggtg aaggcactgt tcagcgtgct caactacgag cgggcgcggc gccccggcct
2401 cctgggcgcc tctgtgctgg gcctggacga tatccacagg gcctggcgca ccttcgtgct
2461 gcgtgtgcgg gcccaggacc cgccgcctga gctgtacttt gtcaaggtgg atgtgacggg
2521 cgcgtacgac accatccccc aggacaggct cacggaggtc atcgccagca tcatcaaacc
2581 ccagaacacg tactgcgtgc gtcggtatgc cgtggtccag aaggccgccc atgggcacgt
2641 ccgcaaggcc ttcaagagcc acgtctctac cttgacagac ctccagccgt acatgcgaca
2701 gttcgtggct cacctgcagg agaccagccc gctgagggat gccgtcgtca tcgagcagag
2761 ctcctccctg aatgaggcca gcagtggcct cttcgacgtc ttcctacgct tcatgtgcca
2821 ccacgccgtg cgcatcaggg gcaagtccta cgtccagtgc caggggatcc cgcagggctc
2881 catcctctcc acgctgctct gcagcctgtg ctacggcgac atggagaaca agctgtttgc
2941 ggggattcgg cgggacgggc tgctcctgcg tttggtggat gatttcttgt tggtgacacc
3001 tcacctcacc cacgcgaaaa ccttcctcag gaccctggtc cgaggtgtcc ctgagtatgg
3061 ctgcgtggtg aacttgcgga agacagtggt gaacttccct gtagaagacg aggccctggg
3121 tggcacggct tttgttcaga tgccggccca cggcctattc ccctggtgcg gcctgctgct
3181 ggatacccgg accctggagg tgcagagcga ctactccagc tatgcccgga cctccatcag
3241 agccagtctc accttcaacc gcggcttcaa ggctgggagg aacatgcgtc gcaaactctt
3301 tggggtcttg cggctgaagt gtcacagcct gtttctggat ttgcaggtga acagcctcca
3361 gacggtgtgc accaacatct acaagatcct cctgctgcag gcgtacaggt ttcacgcatg
3421 tgtgctgcag ctcccatttc atcagcaagt ttggaagaac cccacatttt tcctgcgcgt
3481 catctctgac acggcctccc tctgctactc catcctgaaa gccaagaacg cagggatgtc
3541 gctgggggcc aagggcgccg ccggccctct gccctccgag gccgtgcagt ggctgtgcca
3601 ccaagcattc ctgctcaagc tgactcgaca ccgtgtcacc tacgtgccac tcctggggtc
3661 actcaggaca gcccagacgc agctgagtcg gaagctcccg gggacgacgc tgactgccct
3721 ggaggccgca gccaacccgg cactgccctc agacttcaag accatcctgg actgattaat
3781 taaggatccg cggccgcaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc
3841 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag
3901 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
3961 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
4021 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc
4081 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc
4141 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg
4201 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc
4261 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga
4321 tccaagctgt gaccggcgcc tacgtcgaga tgattgaaca agatggattg cacgcaggtt
4381 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
4441 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
4501 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
4561 ccgcgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
4621 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
4681 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
4741 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
4801 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
4861 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
4921 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
4981 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
5041 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
5101 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgactcgac aatcaacctc
5161 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
5221 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
5281 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
5341 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
5401 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
5461 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
5521 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
5581 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
5641 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
5701 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggtaccttt aagaccaatg
5761 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
5821 ctaattcact cccaacgaag ataagatctg ctttttgctt gtactgggtc tctctggtta
5881 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
5941 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
6001 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
6061 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
6121 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
6181 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
6241 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
6301 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
6361 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact
6421 tttgcagaga ccaaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg
6481 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
6541 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
6601 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
6661 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
6721 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
6781 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
6841 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
6901 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
6961 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
7021 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
7081 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
7141 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
7201 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
7261 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
7321 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
7381 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
7441 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
7501 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
7561 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
7621 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
7681 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
7741 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
7801 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
7861 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
7921 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
7981 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
8041 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
8101 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
8161 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
8221 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
8281 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
8341 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
8401 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
8461 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
8521 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
8581 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
8641 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
8701 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
8761 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
8821 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
8881 gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa
8941 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
9001 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
9061 agtgccaagc tgacgcgtgt agtcttatgc aatactcttg tagtcttgca acatggtaac
9121 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
9181 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
9241 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
9301 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
9361 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
9421 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
9481 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
9541 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
9601 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
9661 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
9721 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
9781 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
9841 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
9901 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
9961 gagcaaaaca aaagtaagac caccgcacag caagcggcca ctgatcttca gacctggagg
10021 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
10081 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
10141 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
10201 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
10261 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
10321 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
10381 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
10441 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
10501 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
10561 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
10621 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
10681 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
10741 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
10801 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
10861 tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
10921 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
10981 aaaattcaaa attttatcga tactagtatt atgcccagta catgacctta tgggactttc
11041 ctacttggca gtacatctac gtattagtca tcgctattac catg
//
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