pLH01

LOCUS       pLH01                9199 bp ds-DNA     circular SYN 06-JUN-2019
DEFINITION  Genome editing for Lactobacillus plantarum WCSF1.
ACCESSION   .
VERSION     .
KEYWORDS    pLH01
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9199)
  TITLE     RecE/T-Assisted Genome Editing in Lactobacillus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 6, 2019 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9199
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(303..336)
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="loxP mutant. Cre-mediated recombination occurs in 
                     the 8-bp core sequence (GCATACAT)."
     protein_bind    complement(1348..1381)
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="loxP mutant. Cre-mediated recombination occurs in 
                     the 8-bp core sequence (GCATACAT)."
     CDS             complement(6616..6627)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     rep_origin      8325..8870
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     8771..8790
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8987..9004
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgtgttgct ttgattgata gccaaaaagc agcagttgat aaagcaatta ctgatattgc
       61 tgaaaaattg taatttataa ataaaaatca ccttttagag gtggtttttt tatttataaa
      121 ttattcgttt gatttcgctt tcgatagaac aatcaaagcg agaataagga agataaatcc
      181 cataagggcg ggagcagaat gtccgagact aatgtaaatt tgtccaccaa ttaaaggacc
      241 gataacgcgc tcgagcctga tagaaacaga agccactgga gcacgtttaa acaatttaaa
      301 tctaccgttc gtataatgta tgctatacga agttatgaca atgtcttagg cgttaaggtc
      361 gttttagccg atggtcgcga agttaagtaa ggtaccatgc agtttaaatt cggtcctcgg
      421 gatatgataa gattaatagt tttagctatt aatctttttt tatttttatt taagaatggc
      481 ttaataaagc ggttactttg gatttttgtg agcttggact agaaaaaaac ttcacaaaat
      541 gctatactag gtagataaaa atttaggagg catatcaaat gaactttaat aaaattgatt
      601 tagacaattg gaagagaaaa gagatattta atcattattt gaaccaacaa acgactttta
      661 gtataaccac agaaattgat attagtgttt tataccgaaa cataaaacaa gaaggatata
      721 aattttaccc tgcatttatt ttcttagtga caagggtgat aaactcaaat acagctttta
      781 gaactggtta caatagcgac ggagagttag gttattggga taagttagag ccactttata
      841 caatttttga tggtgtatct aaaacattct ctggtatttg gactcctgta aagaatgact
      901 tcaaagagtt ttatgattta tacctttctg atgtagagaa atataatggt tcggggaaat
      961 tgtttcccaa aacacctata cctgaaaatg ctttttctct ttctattatt ccatggactt
     1021 catttactgg gtttaactta aatatcaata ataatagtaa ttaccttcta cccattatta
     1081 cagcaggaaa attcattaat aaaggtaatt caatatattt accgctatct ttacaggtac
     1141 atcattctgt ttgtgatggt tatcatgcag gattgtttat gaactctatt caggaattgt
     1201 cagataggcc taatgactgg cttttataat atgagataat gccgactgta cttggatcct
     1261 aaacgcaatt gatgattggt tcggaaggca cgttaggaat cattaccgaa gtaatcgtta
     1321 aactgttgcc gattccgcta gggacccata acttcgtata atgtatgcta tacgaacggt
     1381 acagaccggg gtcgaccctt caatagagtt cttaacgtta atccgaaaaa aactaacgtt
     1441 aatattaaaa aataagatcc gcttgtgaat tatgtataat ttgattagac taaagaatag
     1501 gagaaagtat gatgatattt aaaaaacttt ctcgttaaga taggttgttg gtgagcatgt
     1561 tatatacgga tgtatcggtt tccttaatgc aaaattttgt tgctatctta ttaatttttc
     1621 tattatatag atatattcaa agaaagataa catttaaacg gatcatatta gatattttaa
     1681 tagcgattat tttttcaata ttatatctgt ttatttcaga tgcgtcatta cttgtaatgg
     1741 tattaatgcg attagggtgg cattttcatc aacaaaaaga aaataagata aaaacgactg
     1801 atacagctaa tttaattcta attatcgtga tccagttatt gttagttgcg gttgggacta
     1861 ttattagtca gtttaccata tcgattatca aaagtgattt cagccaaaat atattgaaca
     1921 atagtgcaac agatataact ttattaggta ttttctttgc tgttttattt gacggcttgt
     1981 tctttatatt attgaagaat aagcggactg aattacaaca tttaaatcaa gaaatcattg
     2041 aattttcgtt agaaaaacaa tattttatat ttatatttat tttatttata gtaatagaaa
     2101 ttattttagc agttgggaat cttcaaggag taacagccac gatattatta accattatca
     2161 ttattttttg tgtccttatc gggatgactt tttggcaagt gatgcttttt ttgaaggctt
     2221 attcgattcg ccaagaagcc aatgaccaat tggtccggaa tcaacaactt caagattatc
     2281 tagtcaatat cgaacagcag tacaccgaat tacggcgatt taagcatgat tatcaaaaca
     2341 tcttattatc gttggagagt tttgccgaaa agggcgatca gcaacagttt aaggcgtatt
     2401 accaagaatt attagcacaa cggccaattc aaagtgaaat ccaaggggca gtcattgcac
     2461 aactcgacta cttgaaaaat gatcctattc gaggattagt cattcaaaag tttttggcag
     2521 ccaaacaggc tggtgttact ttaaaattcg aaatgaccga accaatcgaa ttagcaaccg
     2581 ctaatctatt aacggttatt cggattatcg gtattttatt agacaatgcg attgaacaag
     2641 ccgttcaaga aaccgatcaa ttggtgagtt gtgctttctt acaatctgat ggtttaatcg
     2701 aaattacgat tgaaaatacg gccagtcaag ttaagaatct ccaagcattt tcagagttag
     2761 gctattcaac gaaaggcgct ggtcggggga ctggtttagc taatgtgcag gatttgattg
     2821 ccaaacaaac caatttattc ttagaaacac agattgaaaa tagaaagtta cgacagacat
     2881 tgatgattac ggaggaaact taatttgtat cccgtttatt tattagagga tgatttacag
     2941 caacaagcga tttatcagca aattatcgcg aatacgatta tgattaacga atttgcaatg
     3001 actttaacat gcgctgccag tgatactgag acattgttgg cggcaattaa ggatcagcaa
     3061 cgaggtttat tctttttgga tatggaaatt gaggataacc gccaagccgg tttagaagtg
     3121 gcaactaaga ttcggcagat gatgccgttt gcgcaaattg tcttcattac aacccacgag
     3181 gaactgacat tattaacgtt agaacgaaaa atagcgcctt tagattacat tctcaaggac
     3241 caaacaatgg ctgaaatcaa aaggcaattg attgatgatc tattgttagc tgagaagcaa
     3301 aacgaggcgg cagcgtatca ccgagaaaat ttatttagtt ataaaatagg tcctcgcttt
     3361 ttctcattac cattaaagga agttgtttat ttatatactg aaaaagaaaa tccgggtcat
     3421 attaatttgt tagccgttac cagaaaggtt acttttccag gaaatttaaa tgcgctggaa
     3481 gcccaatatc caatgctctt tcggtgtgat aaaagttact tagttaacct atctaatatt
     3541 gccaattatg acagtaaaac acggagttta aaatttgtag atggcagtga ggcaaaagtc
     3601 tcgttccgga aatcacggga actagtggcc aaattaaaac aaatgatgta gcgcctgcag
     3661 cacgccaaat gatcccagta aaaagccacc cgcatggcgg gtggcttttt attagcccta
     3721 gaagggcttc ccacacgcat ttcagcgcct tagtgcctta gtttgtgaat cataggtggt
     3781 atagtcccga aatacccgtc taaggaattg tcagataggc ctaatgactg gcttttataa
     3841 tatgagataa tgccgactgt actttttaca gtcggttttc taatgtcact aacctgcccc
     3901 gttagttgaa gaaggttttt atattacagc tccagatcta ccggtgggcc catattaacg
     3961 tttaaccgat aaagttgaac gttaatattt tttttgcgca gaaatggtaa attgaagcat
     4021 aatagtcttg taaggtattt agctggctgg cgtaaagtat gctttataaa ataatatata
     4081 ggagtatgat tcccatgata ccagcacagg caggtttaaa cgagcattgg cagcaacgta
     4141 acgactcacg tgactgggta cttgacgcag ataactattg ctacgatggt gacgagttcg
     4201 acaaagcaca gttgtttcaa gattacattg ataacaatga ctttgagcag tgggcgactg
     4261 atatgcaggc cgatatgtta agtgccattt gtatcgtcac tttcggttcg actgacgtaa
     4321 gtgtgttgta tccagatcaa ggtgaggaac ctaattggca atggttgatt gatgtgtttg
     4381 gtcagtcccg tctatgggac gagctactgg tacacatcga cacggacacg atgatgacac
     4441 gtctgggcta tcactgggta tcagaggggg aagaagcatg agtaatgagc tagttacgat
     4501 ggttaataac aatattgagg atatgaagaa taatgaaggc ttgtcattac cacctgatta
     4561 ttcagtaggg aatgcattaa acagtgctta cttgattttg agtgatacgt ctaagggcca
     4621 accattactt gataagtgtg accaaggatc agttatcaag gcgttaatga acatggcaat
     4681 tcaaggattg agcccagcta aaaaccaatg ctatttcatt ccttatggta accagttagt
     4741 catgcagcgt tcctattttg gctcaattag cgttgtaaag cgtctttcaa acgttaagga
     4801 tattcaggca caggttgtcc acaaagacga cacgttcaag attggtggta aagatggagt
     4861 gttggtggtt aaagagttcg agccaagctt tgagaaccta gataagccaa ttatcggggc
     4921 ctttgcatgg atcgaagaca tcaacgggaa ccggacatac acggttatga caaaaaagga
     4981 catcgacatc agttggagcc acgctaagac gaagaaggtt caaaacgagt tcccagaaga
     5041 gatggctaaa cggactgtaa ttaatcgagc tgctaagttc tatattaatt caagctcaga
     5101 caacgattta tttgtaaaag cagttaacga aaccactagc aacgaatatg agaatgacga
     5161 aagaaaagac gtaacaccgg ctaagcggtc actagtagct gacgtagcag agaataaagc
     5221 cgagaaggta gaatctgccg aaccagctaa agaacccgtt agaacggctg taaaggaggc
     5281 atcaagcaat gatcaagaac ctgtcaaagg cgaagtcgac cagcaaaacc tcttcgacaa
     5341 cctcggagac tttgacgcca gctaactatt acgatcgctg gacagatcaa tcatttatgt
     5401 cagcaacatg gttcaagaag tttttagcct gtgaagcaga agcgttagcc gagcttactg
     5461 gtaaatggca acctgttcgt gacgctaagg ccttagtagt tggcaattgg cttcatagtt
     5521 attttgaaag caagcaagca cacgaaaagt ttaaagatga gcatccagaa tcaatttcaa
     5581 aacgcggccc tactaaaggg caactaaaga gtgattttaa ggtcgctaat gctatgattc
     5641 agtcgcttac taatgatcat gacttcaatt tactatatca aggcgataag gaagtaattg
     5701 ttactggtga aatcggtggt tatccctgga agggcaagat tgattgcctc aacttgaaac
     5761 aaggctactt cgttgatcta aagacgaccg ctgacatata caaggtgtat tggaatccag
     5821 aaactcgtga gaaagaatcg tttgtatatg cgtataacta cccacttcag atggcagtct
     5881 atcaagagtt gattaagcag caatttggtg tgacgtgtaa gccgtacatc gtggcagtaa
     5941 gcaaacagga tccaccagac aagcaggcta ttgatttacc ggagtaccga cttactaatg
     6001 ctatgaacca ggtattggaa tctcaacagc atcttcaaga tatcattaaa ggcgaagcag
     6061 atcctatcca atgcggacat tgtgcttatt gtcgtagtac caaaaagtta gagagcgtcg
     6121 ttagtgcaga cgacttactc atagattgat cgactcgagg aattcggtac cccgggttcg
     6181 aaggcgccaa gcttcaaatt acagcacgtg ttgctttgat tgatagccaa aaagcagcag
     6241 ttgataaagc aattactgat attgctgaaa aattgtaatt tataaataaa aatcaccttt
     6301 tagaggtggt ttttttattt ataaattatt cgtttgattt cgctttcgat agaacaatca
     6361 aagcgagaat aaggaagata aatcccataa gggcgggagc agaatgtccg agactaattc
     6421 atggatcgat tttttattaa aacgtctcaa aatcgtttct gagacgtttt agcgtttatt
     6481 tcgtttagtt atcggcataa tcgttaaaac aggcgttatc gtagcgtaaa agcccttgag
     6541 cgtagcgtgg ctttgcagcg aagatgttgt ctgttagatt atgaaagccg atgactgaat
     6601 gaaataataa gcgcagcgtc cttctatttc ggttggagga ggctcaaggg agtttgaggg
     6661 aatgaaattc cctcatgggt ttgattttaa aaattgcttg caattttgcc gagcggtagc
     6721 gctggaaaat ttttgaaaaa aatttggaat ttggaaaaaa atggggggaa aggaagcgaa
     6781 ttttgcttcc gtactacgac cccccattaa gaccccccat taagtgccga gtgccaattt
     6841 ttgtgccaaa aacgctctat cccaactggc tcaagggttt gaggggtttt tcaatcgcca
     6901 acgaatcgcc aacgttttcg ccaacgtttt ttataaatct atatttaagt agctttattg
     6961 ttgtttttat gattacaaag tgatacacta attttataaa attatttgat tggagttttt
     7021 taaatggtga tttcagaatc gaaaaaaaga gttatgattt ctctgacaaa agagcaagat
     7081 aaaaaattaa cagatatggc gaaacaaaaa ggtttttcaa aatctgcggt tgcggcgtta
     7141 gctatagaag aatatgcaag aaaggaatca gaacaaaaaa aataagcgaa agctcgcgtt
     7201 tttagaagga tacgagtttt cgctacttgt ttttgataag gtaattatat catggctatt
     7261 aaaaatacta aagctagaaa ttttggattt ttattatatc ctgactcaat tcctaatgat
     7321 tggaaagaaa aattagagag tttgggcgta tctatggctg tcagtccttt acacgatatg
     7381 gacgaaaaaa aagataaaga tacatggaat agtagtgatg ttatacgaaa tggaaagcac
     7441 tataaaaaac cacactatca cgttatatat attgcacgaa atcctgtaac aatagaaagc
     7501 gttaggaaca agattaagcg aaaattgggg aatagttcag ttgctcatgt tgagatactt
     7561 gattatatca aaggttcata tgaatatttg actcatgaat caaaggacgc tattgctaag
     7621 aataaacata tatacgacaa aaaagatatt ttgaacatta atgattttga tattgaccgc
     7681 tatataacac ttgatgaaag ccaaaaaaga gaattgaaga atttactttt agatatagtg
     7741 gatgactata atttggtaaa tacaaaagat ttaatggctt ttattcgcct taggggagcg
     7801 gagtttggaa ttttaaatac gaatgatgta aaagatattg tttcaacaaa ctctagcgcc
     7861 tttagattat ggtttgaggg caattatcag tgtggatata gagcaagtta tgcaaaggtt
     7921 cttgatgctg aaacggggga aataaaatga caaacaaaga aaaagagtta tttgctgaaa
     7981 atgaggaatt aaaaaaagaa attaaggact taaaagagcg tattgaaaga tacagagaaa
     8041 tggaagttga attaagtaca acaatagatt tattgagagg agggattatt gaataaataa
     8101 aagcccccct gacgaaagtc gcactgattg gtgtatcatt tcgtttttct ttttgtgcgg
     8161 acctgcagat gcgatatcat gcgcatgcaa gcttatcgat gataagctgt caaacatgag
     8221 aattacaact tatatcgtat ggggctgact tcaggtgcta catttgaaga gataaattgc
     8281 actgaaatct agaaatattt tatctgatta ataagatgat cttcttgaga tcgttttggt
     8341 ctgcgcgtaa tctcttgctc tgaaaacgaa aaaaccgcct tgcagggcgg tttttcgaag
     8401 gttctctgag ctaccaactc tttgaaccga ggtaactggc ttggaggagc gcagtcacca
     8461 aaacttgtcc tttcagttta gccttaaccg gcgcatgact tcaagactaa ctcctctaaa
     8521 tcaattacca gtggctgctg ccagtggtgc ttttgcatgt ctttccgggt tggactcaag
     8581 acgatagtta ccggataagg cgcagcggtc ggactgaacg gggggttcgt gcatacagtc
     8641 cagcttggag cgaactgcct acccggaact gagtgtcagg cgtggaatga gacaaacgcg
     8701 gccataacag cggaatgaca ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg
     8761 agccgccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc caccactgat
     8821 ttgagcgtca gatttcgtga tgcttgtcag gggggcggag cctatggaaa aacggctttg
     8881 ccgcggccct ctcacttccc tgttaagtat cttcctggca tcttccagga aatctccgcc
     8941 ccgttcgtaa gccatttccg ctcgccgcag tcgaacgacc gagcgtagcg agtcagtgag
     9001 cgaggaagcg gaatatatcc tgtatcacat attctgctga cgcaccggtg cagccttttt
     9061 tctcctgcca catgaagcac ttcactgaca ccctcatcag tgccaacata gtaagccagt
     9121 atacactccg ctagcgctga tgtccggcgg tgcttttgcc gttacgcacc accccgtcag
     9181 tagctgaaca ggagggaca
//