| 出品公司: | Novagen |
|---|---|
| 载体名称: | pETBlue-1 |
| 质粒类型: | 细菌表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 3500 dp |
| 5' 测序引物及序列: | T7 Fwd |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | 蓝/白标识；唯一的MCS |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | 非病毒 |
买家导航
pETBlue1载体基本信息
pETBlue1载体图谱质粒图谱和多克隆位点信息
pETBlue1载体简介
pETBlue1载体序列
LOCUS pETBlue-1 3476 bp ds-DNA circular SYN 23-OCT-2018
DEFINITION Vector for blue/white screening and for inducible bacterial
expression of native unfused proteins.
ACCESSION .
VERSION .
KEYWORDS pETBlue-1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3476)
AUTHORS MilliporeSigma (Novagen)
TITLE Direct Submission
JOURNAL Exported Monday, February 3, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
COMMENT Clone into the EcoRV site for optimal spacing from the RBS.
FEATURES Location/Qualifiers
source 1..3476
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
promoter 1..19
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 20..44
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS complement(54..314)
/codon_start=1
/gene="lacZ (truncated)"
/product="LacZ-alpha fragment of beta-galactosidase"
/note="lacZ-alpha"
/translation="MTMITTPGQEFDIYISFLKLNKIILLANQDLSLAVVLQRRDWENP
GVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWKL"
RBS 265..270
/note="ribosome binding site"
promoter complement(364..392)
/note="tet promoter"
/note="E. coli promoter for tetracycline efflux protein
gene"
terminator 562..648
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 740..767
/note="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 835..882
/note="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
rep_origin 919..1374
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
CDS complement(1489..2349)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2442..3030
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 3429..3448
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator (symmetric)"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG). The
symmetric lac operator was optimized for tight binding of
lac repressor."
ORIGIN
1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gacttacaat
61 ttccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtacg ggcctcttcg
121 ctattacgcc agcttgcgaa cggtgggtgc gctgcaaggc gattaagttg ggtaacgcca
181 ggattctccc agtcacgacg ttgtaaaacg acggccagcg agagatcttg attggctagc
241 agaataattt tgtttaactt taagaaggag atatagatat cgaattcctg cccgggcgtt
301 gtaatcatag tcataatcaa tactcctgac tgcgttagca atttaactgt gataaactac
361 cgcattaaag ctattcgatg ataagctgtc aaacatgata attcttgaag acgaaagggc
421 ctaggctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca
481 tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga
541 gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
601 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg
661 gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact
721 gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa
781 actcttttgt ttatttttct aaatacattc aaatatgtat ccgctgagca ataactagca
841 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
901 tccggattgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
961 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
1021 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
1081 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
1141 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
1201 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
1261 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
1321 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttctggcg
1381 gcacgatggc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
1441 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
1501 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
1561 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
1621 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
1681 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
1741 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
1801 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
1861 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
1921 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
1981 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
2041 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
2101 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
2161 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
2221 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
2281 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
2341 aatactcata ctcttccttt ttcaatcatg accaaaatcc cttaacgtga gttttcgttc
2401 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
2461 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
2521 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
2581 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
2641 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
2701 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
2761 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
2821 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
2881 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
2941 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
3001 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
3061 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
3121 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
3181 cgcagcgagt cagtgagcga ggaagccggc gataatggcc tgcttctcgc cgaaacgttt
3241 ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag
3301 cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag
3361 cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac
3421 gaccggtgaa ttgtgagcgc tcacaattct cgtgacatca taacgtcccg cgaaat
//
