pCMV6-XL4

价格:1500元

联系方式:I47-825O-882O

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pCMV6 XL4载体基本信息

出品公司: OriGene
载体名称: pCMV6-XL4
质粒类型: 哺乳动物表达载体;克隆载体;体外转录载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 4704 bp
5' 测序引物及序列: CMV fwd 5’CGCAAATGGGCGGTAGGCGTG 3’
3' 测序引物及序列: hGH pA rev 5’CCAGCTTGGTTCCCAATAGA 3’
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: --
克隆菌株: DH5α 等
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: --
产品目录号: --
稳定性: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

pCMV6 XL4载体质粒图谱和多克隆位点信息

pCMV6-XL4载体图谱

pCMV6-XL4多克隆位点

pCMV6 XL4载体简介

pCMV6-XL4载体
Selection marker in E. coli: Ampicillin-resistance
Selection marker in mammalian cells: None. For transient transfection only
Promoter for in vivo expression in mammalian cells: CMV promoter
Promoter for in vitro cell free system: T7 (for pCMV6-XL4 and pCMV6-XL5) and SP6 for (pCMV6-XL6)
Cloning sites: EcoRI and SalI. While EcoRI is still preserved, SalI is destroyed upon cloning.
Restriction sites for removing insert: NotI. *Two NotI sites are flanking the cloning sites in the vector.
Cell line suitable for transfection: COS, 293, Hela, CHO, NIH3T3, Mouse L cell, etc.
Transcription termination and polyadenylation signals: from human growth hormone (hGH) gene.

pCMV6 XL4载体序列

LOCUS       pCMV6-XL4                4707 bp    DNA     circular SYN 01-JUL-1998
DEFINITION  Cloning vector pCMV6-XL4, complete sequence.
ACCESSION   AF067196
VERSION     AF067196.1  GI:3273767
KEYWORDS    .
SOURCE      Cloning vector PCMV6-XL4
  ORGANISM  Cloning vector PCMV6-XL4
            other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Cloning vector PCMV6-XL4"
                     /mol_type="genomic DNA"
                     /host="Escherichia coli"
                     /db_xref="taxon:77618"
     promoter        201..926
                     /note="CMV"
     promoter        953..971
                     /note="T7 promoter"
     misc_feature    972..1030
                     /note="multiple cloning site"
     primer_bind     1031..1048
                     /note="M13 reverse primer"
     polyA_signal    1063..1684
                     /note="human growth hormone"
     rep_origin      1740..2047
                     /note="SV40 origin"
     rep_origin      2457..3129
                     /note="ColE1"
     CDS             complement(3274..4134)
                     /codon_start=1
                     /transl_table=11
                     /product="ampicillin resistance protein"
                     /protein_id="AAC24837.1"
                     /db_xref="GI:3273768"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     rep_origin      4267..4679
                     /note="f1"
ORIGIN      
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccgcga attcagatct ggtaccgata tcaagcttgt cgactctaga
     1021 ttgcggccgc ggtcatagct gtttcctgaa catgtgatcc cgggtggcat ccctgtgacc
     1081 cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc
     1141 taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt
     1201 ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg gcctgcgggg
     1261 tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct
     1321 cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca
     1381 tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca
     1441 ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg
     1501 gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttaaaa taactatacc
     1561 agcaggagga cgtccagaca cagcataggc tacctggcca tgcccaaccg gtgggacatt
     1621 tgagttgctt gcttggcact gtcctctcat gcgttgggtc cactcagtag atgcctgttg
     1681 aattgggtac gcggccagct tggctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     1741 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     1801 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     1861 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     1921 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     1981 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     2041 aaaagctcct cgaggaactg aaaaaccaga aagttaattc cctatagtga gtcgtattaa
     2101 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     2161 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     2221 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     2281 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     2341 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     2401 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     2461 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     2521 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     2581 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     2641 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     2701 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     2761 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     2821 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     2881 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     2941 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     3001 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     3061 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     3121 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     3181 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     3241 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     3301 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     3361 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     3421 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     3481 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     3541 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     3601 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     3661 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     3721 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     3781 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     3841 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     3901 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     3961 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     4021 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     4081 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     4141 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     4201 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     4261 acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     4321 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     4381 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     4441 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     4501 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     4561 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     4621 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     4681 atttaacgcg aattttaaca aaatatt
//

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