| 质粒名称: | pREP4-Luc |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 124892 |
| 存储实验室: | Keji Zhao |
|
载体骨架基本信息 |
|
| 载体名称: | 未知 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | -- |
| 筛选标记: | 潮霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | luc |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pREP4-Luc was a gift from Keji Zhao (Addgene plasmid # 124892 ; http://n2t.net/addgene:124892 ; RRID:Addgene_124892) |
| 参考文献部分: | Regulation of CSF1 promoter by the SWI/SNF-like BAF complex. Liu R, Liu H, Chen X, Kirby M, Brown PO, Zhao K. Cell. 2001 Aug 10;106(3):309-18. PubMed 11509180 |
买家导航
pREP4Luc载体基本信息
pREP4Luc载体图谱质粒图谱和多克隆位点信息
pREP4Luc载体简介
pREP4Luc载体序列
LOCUS pREP4-Luc 10257 bp ds-DNA linear SYN 02-MAY-2019
DEFINITION This plasmid contains EBNA sequence..
ACCESSION .
VERSION .
KEYWORDS pREP4-Luc
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10257)
AUTHORS Liu R, Liu H, Chen X, Kirby M, Brown PO, Zhao K
TITLE Regulation of CSF1 promoter by the SWI/SNF-like BAF complex.
JOURNAL Cell. 2001 Aug 10;106(3):309-18.
PUBMED 11509180
REFERENCE 2 (bases 1 to 10257)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 2, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10257
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 1269..3058
/label=oriP
/note="Epstein-Barr virus oriP replication origin (Yates et
al., 2000)"
regulatory 3588..3597
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3594..5246
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/label=luciferase
/note="enhanced luc+ version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
primer_bind complement(3660..3678)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
polyA_signal 5287..5408
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5298..5317)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 5352..5371
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
polyA_signal 5932..5980
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
primer_bind 6005..6024
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
CDS complement(6022..7059)
/codon_start=1
/product="hygromycin B phosphotransferase"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
MGEAN"
promoter complement(7102..7247)
/label=HSV TK promoter
/note="herpes simplex virus thymidine kinase promoter"
rep_origin complement(7678..8266)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7758..7777)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8437..9297)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9060..9079
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9298..9402)
/gene="bla"
/label=AmpR promoter
primer_bind 9470..9488
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 aggggcagga gcaggaggtg gaggccgggg tcgaggaggc agtggaggcc ggggtcgagg
61 aggtagtgga ggccggggtc gaggaggtag tggaggccgc cggggtagag gacgtgaaag
121 agccaggggg ggaagtcgtg aaagagccag ggggagaggt cgtggacgtg gagaaaagag
181 gcccaggagt cccagtagtc agtcatcatc atccgggtct ccaccgcgca ggccccctcc
241 aggtagaagg ccatttttcc accctgtagg ggaagccgat tattttgaat accaccaaga
301 aggtggccca gatggtgagc ctgacgtgcc cccgggagcg atagagcagg gccccgcaga
361 tgacccagga gaaggcccaa gcactggacc ccggggtcag ggtgatggag gcaggcgcaa
421 aaaaggaggg tggtttggaa agcatcgtgg tcaaggaggt tccaacccga aatttgagaa
481 cattgcagaa ggtttaagag ctctcctggc taggagtcac gtagaaagga ctaccgacga
541 aggaacttgg gtcgccggtg tgttcgtata tggaggtagt aagacctccc tttacaacct
601 aaggcgagga actgcccttg ctattccaca atgtcgtctt acaccattga gtcgtctccc
661 ctttggaatg gcccctggac ccggcccaca acctggcccg ctaagggagt ccattgtctg
721 ttatttcatg gtctttttac aaactcatat atttgctgag gttttgaagg atgcgattaa
781 ggaccttgtt atgacaaagc ccgctcctac ctgcaatatc agggtgactg tgtgcagctt
841 tgacgatgga gtagatttgc ctccctggtt tccacctatg gtggaagggg ctgccgcgga
901 gggtgatgac ggagatgacg gagatgaagg aggtgatgga gatgagggtg aggaagggca
961 ggagtgatgt aacttgttag gagacgccct caatcgtatt aaaagccgtg tattcccccg
1021 cactaaagaa taaatcccca gtagacatca tgcgtgctgt tggtgtattt ctggccatct
1081 gtcttgtcac cattttcgtc ctcccaacat ggggcaattg ggcataccca tgttgtcacg
1141 tcactcagct ccgcgctcaa caccttctcg cgttggaaaa cattagcgac atttacctgg
1201 tgagcaatca gacatgcgac ggctttagcc tggcctcctt aaattcacct aagaatggga
1261 gcaaccagca ggaaaaggac aagcagcgaa aattcacgcc cccttgggag gtggcggcat
1321 atgcaaagga tagcactccc actctactac tgggtatcat atgctgactg tatatgcatg
1381 aggatagcat atgctacccg gatacagatt aggatagcat atactaccca gatatagatt
1441 aggatagcat atgctaccca gatatagatt aggatagcct atgctaccca gatataaatt
1501 aggatagcat atactaccca gatatagatt aggatagcat atgctaccca gatatagatt
1561 aggatagcct atgctaccca gatatagatt aggatagcat atgctaccca gatatagatt
1621 aggatagcat atgctatcca gatatttggg tagtatatgc tacccagata taaattagga
1681 tagcatatac taccctaatc tctattagga tagcatatgc tacccggata cagattagga
1741 tagcatatac tacccagata tagattagga tagcatatgc tacccagata tagattagga
1801 tagcctatgc tacccagata taaattagga tagcatatac tacccagata tagattagga
1861 tagcatatgc tacccagata tagattagga tagcctatgc tacccagata tagattagga
1921 tagcatatgc tatccagata tttgggtagt atatgctacc catggcaaca ttagcccacc
1981 gtgctctcag cgacctcgtg aatatgagga ccaacaaccc tgtgcttggc gctcaggcgc
2041 aagtgtgtgt aatttgtcct ccagatcgca gcaatcgcgc ccctatcttg gcccgcccac
2101 ctacttatgc aggtattccc cggggtgcca ttagtggttt tgtgggcaag tggtttgacc
2161 gcagtggtta gcggggttac aatcagccaa gttattacac ccttatttta cagtccaaaa
2221 ccgcagggcg gcgtgtgggg gctgacgcgt gcccccactc cacaatttca aaaaaaagag
2281 tggccacttg tctttgttta tgggccccat tggcgtggag ccccgtttaa ttttcggggg
2341 tgttagagac aaccagtgga gtccgctgct gtcggcgtcc actctctttc cccttgttac
2401 aaatagagtg taacaacatg gttcacctgt cttggtccct gcctgggaca catcttaata
2461 accccagtat catattgcac taggattatg tgttgcccat agccataaat tcgtgtgaga
2521 tggacatcca gtctttacgg cttgtcccca ccccatggat ttctattgtt aaagatattc
2581 agaatgtttc attcctacac tagtatttat tgcccaaggg gtttgtgagg gttatattgg
2641 tgtcatagca caatgccacc actgaacccc ccgtccaaat tttattctgg gggcgtcacc
2701 tgaaaccttg ttttcgagca cctcacatac accttactgt tcacaactca gcagttattc
2761 tattagctaa acgaaggaga atgaagaagc aggcgaagat tcaggagagt tcactgcccg
2821 ctccttgatc ttcagccact gcccttgtga ctaaaatggt tcactaccct cgtggaatcc
2881 tgaccccatg taaataaaac cgtgacagct catggggtgg gagatatcgc tgttccttag
2941 gaccctttta ctaaccctaa ttcgatagca tatgcttccc gttgggtaac atatgctatt
3001 gaattagggt tagtctggat agtatatact actacccggg aagcatatgc tacccgttta
3061 gggttaacaa gggggcctta taaacactat tgctaatgcc ctcttgaggg tccgcttatc
3121 ggtagctaca caggcccctc tgattgacgt tggtgtagcc tcccgtagtc ttcctgggcc
3181 cctgggaggt acatgtcccc cagcattggt gtaagagctt cagccaagag ttacacataa
3241 aggcaatgtt gtgttgcagt ccacagactg caaagtctgc tccaggatga aagccactca
3301 gtgttggcaa atgtgcacat ccatttataa ggatgtcaac tacagtcaga gaaccccttt
3361 gtgtttggtc cccccccgtg tcacatgtgg aacagggccc agttggcaag ttgtaccaac
3421 caactgaagg gattacatgc actgccccgc gaagaagggg cagagatgtc gtagtcaggt
3481 ttagttcgtc cggggcgggg atcgatcctc tagagtcgag atctggtacc agctgctagc
3541 ggccgcgaag gcctcgagaa gcttggcatt ccggtactgt tggtaaagcc accatggaag
3601 acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat ggaaccgctg
3661 gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca attgctttta
3721 cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg tccgttcggt
3781 tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc gtatgcagtg
3841 aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga gttgcagttg
3901 cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc atttcgcagc
3961 ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaagc
4021 tcccaatcat ccaaaaaatt attatcatgg attctaaaac ggattaccag ggatttcagt
4081 cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac gattttgtgc
4141 cagagtcctt cgatagggac aagacaattg cactgatcat gaactcctct ggatctactg
4201 gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg cgtgagattc tcgcatgcca
4261 gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt gttgttccat
4321 tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga tttcgagtcg
4381 tcttaatgta tagatttgaa gaagagctgt ttctgaggag ccttcaggat tacaagattc
4441 aaagtgcgct gctggtgcca accctattct ccttcttcgc caaaagcact ctgattgaca
4501 aatacgattt atctaattta cacgaaattg cttctggtgg cgctcccctc tctaaggaag
4561 tcggggaagc ggttgccaag aggttccatc tgccaggtat caggcaagga tatgggctca
4621 ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg ggcgcggtcg
4681 gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg aaaacgctgg
4741 gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc ggttatgtaa
4801 acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat tctggagaca
4861 tagcttactg ggacgaagac gaacacttct tcatcgttga ccgcctgaag tctctgatta
4921 agtacaaagg ctatcaggtg gctcccgctg aattggaatc catcttgctc caacacccca
4981 acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg
5041 ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg gattacgtcg
5101 ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac
5161 cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca
5221 agaagggcgg aaagatcgcc gtgtaattct agagtcgggg cggccggccg cttcgagcag
5281 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
5341 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
5401 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
5461 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgtcga
5521 ctagcttggc acgccagaaa tccgcgcggt ggtttttggg ggtcgggggt gtttggcagc
5581 cacagacgcc cggtgttcgt gtcgcgccag tacatgcggt ccatgcccag gccatccaaa
5641 aaccatgggt ctgtctgctc agtccagtcg tggacctgac cccacgcaac gcccaaaata
5701 ataaccccca cgaaccataa accattcccc atgggggacc ccgtccctaa cccacggggc
5761 cagtggctat ggcagggcct gccgccccga cgttggctgc gagccctggg ccttcacccg
5821 aacttggggg gtggggtggg gaaaaggaag aaacgcgggc gtattggccc caatggggtc
5881 tcggtggggt atcgacagag tgccagccct gggaccgaac cccgcgttta tgaacaaacg
5941 acccaacacc cgtgcgtttt attctgtctt tttattgccg tcatagcgcg ggttccttcc
6001 ggtattgtct ccttccgtgt ttcagttagc ctcccccatc tcccgatccg gacgagtgct
6061 ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc agacggccgc
6121 gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga cgattgcgtc
6181 gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc tctgatagag
6241 ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg caagctccgg
6301 atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg gcctccagaa
6361 gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc agtcaatgac
6421 cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg cgtgcacgag
6481 gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag cctgcgcgac
6541 ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg gatcagcaat
6601 cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc
6661 gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatggcct ccgcgaccgg
6721 ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac cctgtgcacg
6781 gcgggagatg caataggtca ggctctcgct gaattcccca atgtcaagca cttccggaat
6841 cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga aaccatcggc
6901 gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga aagcacgaga
6961 ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa
7021 cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tctcattgcc ccccgggatc
7081 tgcggcacgc tgttgacgct gttaagcggg tcgctgcagg gtcgctcggt gttcgaggcc
7141 acacgcgtca ccttaatatg cgaagtggac ctcggaccgc gccgccccga ctgcatctgc
7201 gtgttcgaat tcgccaatga caagacgctg ggcggggttt gtgtcatcat agaactaaag
7261 acatgcaaat atatttcttc cggggacacc gccagcaaac gcgagcaacg ggccacgggg
7321 atgaagcagg gcggcacctc gctaacggat tcaccactcc aagaattgga gccaatcaat
7381 tcttgcggag aactgtgaat gcgcaaacca acccttggca gaacatatcc atcgcgtccg
7441 ccatctccag cagccgcacg cggcgcatct cggggccgac gcgctgggct acgtcttgct
7501 ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg
7561 catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg
7621 acagcttcaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
7681 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
7741 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
7801 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
7861 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
7921 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
7981 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
8041 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
8101 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
8161 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
8221 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
8281 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
8341 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
8401 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
8461 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
8521 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
8581 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
8641 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
8701 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat
8761 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
8821 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
8881 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
8941 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
9001 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga
9061 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
9121 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
9181 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
9241 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
9301 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
9361 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
9421 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
9481 cacgaggccc tttcgtcttc aagaattctc atgtttgaca gcttatcatc gataagctga
9541 tcctcacagg ccgcacccag cttttcttcc gttgccccag tagcatctct gtctggtgac
9601 cttgaagagg aagaggaggg gtcccgagaa tccccatccc taccgtccag caaaaagggg
9661 gacgaggaat ttgaggcctg gcttgaggct caggacgcaa atcttgagga tgttcagcgg
9721 gagttttccg ggctgcgagt aattggtgat gaggacgagg atggttcgga ggatggggaa
9781 ttttcagacc tggatctgtc tgacagcgac catgaagggg atgagggtgg gggggctgtt
9841 ggagggggca ggagtctgca ctccctgtat tcactgagcg tcgtctaata aagatgtcta
9901 ttgatctctt ttagtgtgaa tcatgtctga cgaggggcca ggtacaggac ctggaaatgg
9961 cctaggagag aagggagaca catctggacc agaaggctcc ggcggcagtg gacctcaaag
10021 aagagggggt gataaccatg gacgaggacg gggaagagga cgaggacgag gaggcggaag
10081 accaggagcc ccgggcggct caggatcagg gccaagacat agagatggtg tccggagacc
10141 ccaaaaacgt ccaagttgca ttggctgcaa agggacccac ggtggaacag gagcaggagc
10201 aggagcggga ggggcaggag caggaggggc aggagcagga ggaggggcag gagcagg
//
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pREP4Luc载体质粒应用举例
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