pLVX-AcGFP1-N1

价格:1200元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pLVX-AcGFP1-N1, pLVX AcGFP1 N1
质粒类型: 慢病毒表达载体
高拷贝/低拷贝: 高拷贝
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 8787 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: C-AcGFP1
载体抗性: 氨苄
筛选标记: 嘌呤霉素(Puromycin)
克隆菌株: Stbl3
备注: --
产品目录号: 632154
稳定性: 稳表达
组成型: 组成型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVX-AcGFP1-N1载体图谱



pLVX-AcGFP1-N1多克隆位点

pLVX-AcGFP1-N1载体特征

载体简介

Description
pLVX-AcGFP1-N1 is an HIV-1-based, lentiviral expression vector that allows you to express your gene of interest fused to AcGFP1, a green fl uorescent protein derived from Aequorea coerulescens. Genes cloned into the multiple cloning site (MCS), located upstream of the AcGFP1 coding sequence, are expressed as N-terminal fusions of the AcGFP1 protein. Expression of the fusion protein is driven by the constitutively active human cytomegalovirus immediate early promoter (PCMV IE) located just upstream of the MCS. Lentiviral particles derived from the vector allow the expression of AcGFP1 fusion proteins in virtually any cell type, including primary cells. The unmodifi ed vector expresses AcGFP1, and may be used to produce marker virus to optimize infection protocols.

pLVX-AcGFP1-N1 contains all of the viral processing elements necessary for the production of replication-incompetent lentivirus, as well as elements to improve viral titer, transgene expression, and overall vector function. The woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) promotes RNA processing events and enhances nuclear export of viral and transgene RNA (1), leading to increased viral titers from packaging cells, and enhanced expression of your gene of interest in target cells. In addition, the vector includes a Rev-response element (RRE), which further increases viral titers by enhancing the transport of unspliced viral RNA out of the nucleus (2). Finally, pLVX-AcGFP1-N1 also contains a central polypurine tract (cPPT) element that increases nuclear importation of the viral genome during target cell infection, resulting in improved vector integration and more efficient transduction (3).

In addition to lentiviral elements, pLVX-AcGFP1-N1 contains a puromycin resistance gene (Puror) under the control of the murine phosphoglycerate kinase (PGK) promoter (PPGK) for the selection of stable transductants. The vector also contains a pUC origin of replication and an E. coli ampicillin resistance gene (Ampr) for propagation and selection in bacteria.

Use
pLVX-AcGFP1-N1 constitutively expresses your gene of interest from PCMV IE when transduced into target cells. Before the vector can be transduced into cells, however, it must be transfected into 293T packaging cells with our Lenti-X™ HT Packaging System (Cat. Nos. 632160 and 632161). This packaging system allows you to safely produce high titer, infectious, replication-incompetent, VSV-G pseudotyped lentiviral particles that can infect a wide range of cell types, including non-dividing and primary cells (4).

载体序列

LOCUS       pLVX-AcGFP1-N1   8787 bp    DNA   circular             9-NOV-2009
COMMENT     Created by Clontech Laboratories Inc. 
COMMENT     Cat. No. 632154
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=Packaging\signal
     misc_feature    2028..2151
                     /label=cPPT/CTS
     promoter        2185..2787
                     /label=CMV\IE\Promoter
     misc_feature    4755..5346
                     /label=WPRE
     rep_origin      complement(6655..7328)
                     /label=pUC\origin
     CDS             complement(7473..8469)
                     /label=Amp(R)
     misc_binding    1303..1536
                     /label=RRE
     misc_binding    636..653
                     /label=PBS
     LTR             5549..6185
                     /label=3'\LTR
     misc_feature    2796..2868
                     /label=MCS
     CDS             2875..3591
                     /label=AcGFP1
     promoter        3613..4121
                     /label=PGK\Promoter
     CDS             4142..4741
                     /label=Puro(R)
BASE COUNT     2188 a      2273 c      2312 g      2014 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 
     2821 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccacc ggtcatggtg 
     2881 agcaagggcg ccgagctgtt caccggcatc gtgcccatcc tgatcgagct gaatggcgat 
     2941 gtgaatggcc acaagttcag cgtgagcggc gagggcgagg gcgatgccac ctacggcaag 
     3001 ctgaccctga agttcatctg caccaccggc aagctgcctg tgccctggcc caccctggtg 
     3061 accaccctga gctacggcgt gcagtgcttc tcacgctacc ccgatcacat gaagcagcac 
     3121 gacttcttca agagcgccat gcctgagggc tacatccagg agcgcaccat cttcttcgag 
     3181 gatgacggca actacaagtc gcgcgccgag gtgaagttcg agggcgatac cctggtgaat 
     3241 cgcatcgagc tgaccggcac cgatttcaag gaggatggca acatcctggg caataagatg 
     3301 gagtacaact acaacgccca caatgtgtac atcatgaccg acaaggccaa gaatggcatc 
     3361 aaggtgaact tcaagatccg ccacaacatc gaggatggca gcgtgcagct ggccgaccac 
     3421 taccagcaga atacccccat cggcgatggc cctgtgctgc tgcccgataa ccactacctg 
     3481 tccacccaga gcgccctgtc caaggacccc aacgagaagc gcgatcacat gatctacttc 
     3541 ggcttcgtga ccgccgccgc catcacccac ggcatggatg agctgtacaa gtgagcggcc 
     3601 gcgactctag ataattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat 
     3661 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac 
     3721 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac 
     3781 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga 
     3841 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc 
     3901 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct 
     3961 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg 
     4021 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt 
     4081 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac 
     4141 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt 
     4201 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga 
     4261 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga 
     4321 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga 
     4381 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg 
     4441 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga 
     4501 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg 
     4561 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct 
     4621 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc 
     4681 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg 
     4741 accgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc 
     4801 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg 
     4861 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc 
     4921 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg 
     4981 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg 
     5041 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga 
     5101 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct 
     5161 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg 
     5221 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc 
     5281 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc 
     5341 cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa 
     5401 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg 
     5461 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct 
     5521 tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca 
     5581 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta 
     5641 cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc 
     5701 agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc 
     5761 tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag 
     5821 ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg 
     5881 atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg 
     5941 cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta 
     6001 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc 
     6061 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt 
     6121 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta 
     6181 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat 
     6241 cagagagtga gaggccttga cattgctagc gttttaccgt cgacctctag ctagagcttg 
     6301 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 
     6361 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 
     6421 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 
     6481 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 
     6541 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 
     6601 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 
     6661 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 
     6721 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 
     6781 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 
     6841 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 
     6901 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 
     6961 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 
     7021 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 
     7081 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 
     7141 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 
     7201 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 
     7261 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 
     7321 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 
     7381 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 
     7441 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 
     7501 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 
     7561 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 
     7621 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 
     7681 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 
     7741 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 
     7801 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 
     7861 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 
     7921 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 
     7981 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 
     8041 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 
     8101 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 
     8161 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 
     8221 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 
     8281 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 
     8341 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 
     8401 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 
     8461 cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata 
     8521 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 
     8581 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa 
     8641 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt 
     8701 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg 
     8761 ttaaatatgt actacaaact tagtagt 
//

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