| 质粒名称: | GW1-Peredox-mCitrine |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 32386 |
| 存储实验室: | Gary Yellen |
|
载体骨架基本信息 |
|
| 载体名称: | GW1 |
| 载体出品公司: | British Biotechnology |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 5300 bp |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Peredox-mCitrine |
| 插入大小: | 2800 bp |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | PspOMI(未破坏) |
| 3' 克隆位点: | SalI (克隆期间被破坏) |
| 5' 测序引物 | |
| 3' 测序引物 | EGFP-R |
|
文献引用方法 |
|
| 材料和方法部分: | GW1-Peredox-mCitrine was a gift from Gary Yellen (Addgene plasmid # 32386) |
| 参考文献部分: | Imaging Cytosolic NADH-NAD(+) Redox State with a Genetically Encoded Fluorescent Biosensor. Hung YP, Albeck JG, Tantama M, Yellen G. Cell Metab. 2011 Oct 5;14(4):545-54. 10.1016/j.cmet.2011.08.012 PubMed 21982714 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS GW1-Peredox-mCitrine 8054 bp DNA SYN
DEFINITION GW1-Peredox-mCitrine
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..8054
/organism="GW1-Peredox-mCitrine"
/mol_type="other DNA"
CDS 121..2898
/label="ORF frame 1"
CDS complement(934..1485)
/label="ORF frame 3"
CDS complement(1683..2222)
/label="ORF frame 1"
CDS complement(1978..2919)
/label="ORF frame 3"
gene 2182..2895
/label="EGFP (variant)"
/gene="EGFP (variant)"
misc_feature complement(2224..2245)
/label="EGFP_N_primer"
misc_feature 2832..2853
/label="EGFP_C_primer"
terminator 3023..3154
/label="SV40_PA_terminator"
misc_feature 3111..3130
/label="EBV_rev_primer"
promoter complement(3174..3192)
/label="M13_reverse_primer"
misc_feature complement(3191..3213)
/label="M13_pUC_rev_primer"
promoter complement(3227..3256)
/label="lac_promoter"
rep_origin complement(3565..4184)
/label="pBR322_origin"
gene complement(4339..5199)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(4339..5199)
/label="ORF frame 3"
promoter complement(5278..5306)
/label="AmpR_promoter"
misc_feature complement(5465..5487)
/label="pGEX_3_primer"
misc_feature complement(5674..5693)
/label="SV40pro_F_primer"
misc_feature complement(5724..5867)
/label="lacZ_a"
CDS complement(5724..6251)
/label="ORF frame 1"
misc_feature 5838..5860
/label="M13_pUC_fwd_primer"
promoter 5853..5869
/label="M13_forward20_primer"
promoter 6419..6995
/label="CMV_immearly_promoter"
misc_feature 6498..6785
/label="CAG_enhancer"
misc_feature 6952..6972
/label="CMV_fwd_primer"
promoter 6953..7022
/label="CMV_promoter"
promoter 6965..7084
/label="CMV2_promoter"
misc_feature 6996..7015
/label="pCEP_fwd_primer"
misc_feature 6998..7022
/label="LNCX_primer"
CDS complement(7490..7948)
/label="ORF frame 2"
CDS complement(7851..762)
/label="ORF frame 3"
ORIGIN
1 tccgagtccg ataccgataa agattctgaa tcggagtgag tgccgcgtcc gagagcgttc
61 cctgtccacg tccaccatcg gcggagcagg tgtgcctgag gcctctagat tgggcccacc
121 atggttgcta gcaaagttcc tgaagcagcc atttccagac tgattactta cctgagaatt
181 ctggaagagc tggaagccca aggtgtacac cgcaccgcct ccgagcaact gggcgagctg
241 gcccaggtca ccgccttcca ggttgataag gacctgtcct acttcggctc ctacggcact
301 gacggcgttg gctacactgt accggtcctc aagagagaac tgcgccatat cctcggtctg
361 aaccgcaaat ggggcctgtg catcgtgggg atgggccgcc tgggatccgc tcttgctgat
421 tggcctggtt ttggcgagag cttcgagctg cgcggtttct tcgatgttga cccaggtatg
481 gttggtcggc cggttcgcgg tggtgttatc gaacacgttg atctgctgcc ccagcgcgta
541 cctggtcgca tcgaaatcgc tctgctgacg gttccacgcg aggcagcaca gaaggctgcc
601 gacctgctgg ttgccgctgg catcaagggc atcctgaact tcgctccagt tgtactggag
661 gttcccaagg aagtggctgt tgagaacgtt gacatcctgg ccggtctgac gcgtctgagc
721 tttgccattc tgaaccccac gtggagcgca gcaggtgggc atggtttcac tgctcacaac
781 gtgtatatca tggctgacaa acagaagaac ggtatcaagg ccaacttcaa gatccgccac
841 aacatcgaag acggcggcgt tcagctggct gatcactacc agcagaacac cccaatcggc
901 gacggccctg ttctgctgcc tgacaaccac tacttaagca tccagtctaa actgtccaaa
961 gacccaaacg agaagcgcga ccacatggtt ctgctggaat ttgttaccgc cgctggcatc
1021 acccacggca tggacgaact gtacaagggc ggcaccgggg gatctatggt ttccaaaggc
1081 gaggaactgt tcactggtgt tgttcctatc ctggttgaac tggacggcga cgttaacggc
1141 cacaaattca gcgttagcgg ggaaggcgaa ggagacgcta cctacggcaa gctgactctg
1201 aagttcatct gcaccactgg caagctgcct gttccttggc ctaccctggt tactaccttc
1261 tcctatggtg ttatggtttt cgccagatat cctgaccaca tgaagcagca cgacttcttc
1321 aagagcgcca tgcctgaggg ctacgttcag gaacgcacca tctttttcaa ggacgacggc
1381 aactataaga cacgcgctga ggttaagttc gagggcgaca ctctggttaa ccgcatcgag
1441 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg gccataagct tgaatataac
1501 accaaggtac ctgaagccgc catcagccgc ctcatcactt acctccgcat cctggaggag
1561 ctcgaagccc aaggcgttca ccgcactgca agcgagcagc tgggcgaact ggcacaggtt
1621 acagcattcc aggttgacga agatctgtcc tatttcggtt cctacggcac cgacggtgtt
1681 ggctacacag tccctgtcct gaagcgcgag ctgcgccaca tcctgggcct gaaccgcaag
1741 tggggcttat gcatcgttgg catgggccgc ctcggtagtg cactggcaga ctggccgggc
1801 tttggtgaat ctttcgaact gcgcggcttc ttcgacgttg atccaggcat ggttgggcgc
1861 cctgttcgcg gtggcgttat cgagcatgtt gacctcctgc cacagcgcgt cccgggtcgc
1921 atcgagatcg ccctgctgac cgttcctcgc gaagctgcac agaaggcagc cgacttacta
1981 gtcgccgctg gtatcaaggg aattctgaac ttcgcccctg ttgttctgga ggttcctaag
2041 gaagttgctg ttgaaaacgt tgatttcctg gccggcctga cccgcctgag cttcgcaatc
2101 ctgaatccta agtggcgcga agaaatgatg ggttccggaa ctgggggcaa cgcctctgac
2161 ggtggtgggt ctggtggtat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
2221 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
2281 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
2341 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgatgtg cttcgcccgc
2401 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
2461 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
2521 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
2581 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
2641 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
2701 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
2761 ctgctgcccg acaaccacta cctgagctac cagtccaaac tgagcaaaga ccccaacgag
2821 aagcgcgatc acatggtcct gctggagttc gtgaccgctg ctgggatcac tctgggtatg
2881 gatgaactgt acaagtaact cgacgaattc attgatcata atcagccata ccacatttgt
2941 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
3001 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
3061 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
3121 caaactcatc aatgtatctt atcatgtctg gatccgtacc gagctcgcgt aatcatggtc
3181 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
3241 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
3301 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
3361 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
3421 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
3481 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
3541 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
3601 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
3661 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
3721 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
3781 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
3841 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
3901 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
3961 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
4021 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
4081 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
4141 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
4201 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
4261 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
4321 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
4381 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
4441 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
4501 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
4561 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
4621 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
4681 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
4741 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
4801 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
4861 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
4921 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
4981 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
5041 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
5101 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
5161 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaataagc
5221 ggccgcggcc atgccggcca ctagtctcga gttattattg aagcatttat cagggttatt
5281 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
5341 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
5401 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
5461 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
5521 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
5581 actatgcggc atcagagcag attgtactga gagtgcacca tatgaagacg tcgcctcctc
5641 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
5701 ttagtcagcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
5761 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
5821 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccatctgcag
5881 tgaataataa aatgtgtgtt tgtccgaaat acgcgtttga gatttctgtc ccgactaaat
5941 tcatgtcgcg cgatagtggt gtttatcgcc gatagagatg gcgatattgg aaaaatcgat
6001 atttgaaaat atggcatatt gaaaatgtcg ccgatgtgag tttctgtgta actgatatcg
6061 ccatttttcc aaaagttgat ttttgggcat acgcgatatc tggcgatacg cttatatcgt
6121 ttacggggga tggcgataga cgcctttggt gacttgggcg attctgtgtg tcgcaaatat
6181 cgcagtttcg atataggtga cagacgatat gaggctatat cgccgataga ggcgacatca
6241 agctggcaca tggccaatgc atatcgatct atacattgaa tcaatattgg ccattagcca
6301 tattattcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt
6361 atccatatca taatatgtac atttatattg gctcatgtcc aacattaccg ccatgttgac
6421 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
6481 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
6541 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
6601 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
6661 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
6721 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
6781 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
6841 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
6901 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
6961 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
7021 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
7081 gcctccgcgg ccgggaacgg tgcattggaa cgcggattcc ccgtgccaag agtgacgtaa
7141 gtaccgccta tagagtctat aggcccaccc ccttggcttc ttatgcatgc tatactgttt
7201 ttggcttggg gtctatacac ccccgcttcc tcatgttata ggtgatggta tagcttagcc
7261 tataggtgtg ggttattgac cattattgac cactccccta ttggtgacga tactttccat
7321 tactaatcca taacatggct ctttgccaca actctcttta ttggctatat gccaatacac
7381 tgtccttcag agactgacac ggactctgta tttttacagg atggggtctc atttattatt
7441 tacaaattca catatacaac accaccgtcc ccagtgcccg cagtttttat taaacataac
7501 gtgggatctc cacgcgaatc tcgggtacgt gttccggaca tgggctcttc tccggtagcg
7561 gcggagcttc tacatccgag ccctgctccc atgcctccag cgactcatgg tcgctcggca
7621 gctccttgct cctaacagtg gaggccagac ttaggcacag cacgatgccc accaccacca
7681 gtgtgccgca caaggccgtg gcggtagggt atgtgtctga aaatgagctc ggggagcggg
7741 cttgcaccgc tgacgcattt ggaagactta aggcagcggc agaagaagat gcaggcagct
7801 gagttgttgt gttctgataa gagtcagagg taactcccgt tgcggtgctg ttaacggtgg
7861 agggcagtgt agtctgagca gtactcgttg ctgccgcgcg cgccaccaga cataatagct
7921 gacagactaa cagactgttc ctttccatgg gtcttttctg cagtcaccgt ccttgacacg
7981 aagcttcccg gccacagcaa tcgatccatg acacaaccag tgacaagcag tcccccagtg
8041 aaaccgcatc cgca
//
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