| 质粒名称: | p6sheLLr |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 37318 |
| 存储实验室: | John Schiller |
|
载体骨架基本信息 |
|
| 载体名称: | custom |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 博莱霉素 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | HPV6L1+L2,p6LLw2 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | p6sheLLr was a gift from John Schiller (Addgene plasmid # 37318 ; http://n2t.net/addgene:37318 ; RRID:Addgene_37318) |
| 参考文献部分: | Cross-neutralization of cutaneous and mucosal Papillomavirus types with anti-sera to the amino terminus of L2. Pastrana DV, Gambhira R, Buck CB, Pang YY, Thompson CD, Culp TD, Christensen ND, Lowy DR, Schiller JT, Roden RB. Virology. 2005 Jul 5;337(2):365-72. 10.1016/j.virol.2005.04.011 PubMed 15885736 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS p6sheLLr 10776 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS p6sheLLr
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10776)
AUTHORS Pastrana DV, Gambhira R, Buck CB, Pang YY, Thompson CD, Culp TD,
Christensen ND, Lowy DR, Schiller JT, Roden RB
TITLE Cross-neutralization of cutaneous and mucosal Papillomavirus types
with anti-sera to the amino terminus of L2.
JOURNAL Virology. 2005 Jul 5;337(2):365-72.
PUBMED 15885736
REFERENCE 2 (bases 1 to 10776)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10776
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 2041..2629
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(2094..2114)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(2512..2523)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(2666..2683)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 2672..2896
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2942..3370
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3029..3048)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3239..3260
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(3379..3399)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
CDS 5328..5696
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="KLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDVT
LFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGREF
ALRDPAGNCVHFVAEEQD"
polyA_signal 5826..5947
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5863..5882)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5917..5936
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(5996..6012)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5996..6012)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6009..6031)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6020..6036
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6044..6074)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6089..6110
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6227..6244)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6398..6983)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6478..6497)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7154..8014)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7777..7796
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8015..8119)
/gene="bla"
/label=AmpR promoter
primer_bind complement(8194..8213)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 8385..8764
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 8766..8968
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
LTR 8993..9261
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
ORIGIN
1 tcgaggaagc ttaaaacagc tctggggttg tacccacccc agaggcccac gtggcggcta
61 gtactccggt attgcggtac ccttgtacgc ctgttttata ctcccttccc gtaacttaga
121 cgcacaaaac caagttcaat agaagggggt acaaaccagt accaccacga acaagcactt
181 ctgtttcccc ggtgatgtcg tatagactgc ttgcgtggtt gaaagcgacg gatccgttat
241 ccgcttatgt acttcgagaa gcccagtacc acctcggaat cttcgatgcg ttgcgctcag
301 cactcaaccc cagagtgtag cttaggctga tgagtctgga catccctcac cggtgacggt
361 ggtccaggct gcgttggcgg cctacctatg gctaacgcca tgggacgcta gttgtgaaca
421 aggtgtgaag agcctattga gctacataag aatcctccgg cccctgaatg cggctaatcc
481 caacctcgga gcaggtggtc acaaaccagt gattggcctg tcgtaacgcg caagtccgtg
541 gcggaaccga ctactttggg tgtccgtgtt tccttttatt ttattgtggc tgcttatggt
601 gacaatcaca gattgttatc ataaagcgaa ttggattgcg gcccatggcc cactcccgcg
661 cacgccgcag gaaacgcgcc agcgccaccc aactgtacca gacctgcaag ttgaccggca
721 cctgtccacc cgacgtcatc cccaaggtcg aacacaacac aatcgccgac cagatcctga
781 agtggggctc cctcggcgtg ttcttcggcg gcctcggcat cgggacaggc agtgggaccg
841 gcggcaggac cggctacgtc ccactgggca ccagcgccaa gcccagcatc acctccggcc
901 caatggccag gccccccgtc gtcgtcgaac ccgtcgcacc cagcgacccc agcatcgtca
961 gcctgatcga ggagagcgcc atcatcaatg ccggagcccc tgagatcgtc ccacccgccc
1021 acggcggctt caccatcacc agtagcgaga ccaccacacc cgccatcctc gacgtcagcg
1081 tcacctccca caccaccacc tccatcttcc gcaaccccgt gttcaccgag cccagcgtca
1141 cccagccaca gcccccggtc gaagccaacg gccacatcct gatcagcgcc ccaaccatca
1201 ccagccatcc catcgaagag atccccctgg acaccttcgt catcagtagc tccgactcag
1261 gccccaccag ttccacaccc gtccccggca ccgccccacg ccccagggtc gggctgtact
1321 cccgcgccct ccatcaggtc caggtcaccg atcccgcctt cttgagtacc ccccaaaggc
1381 tgatcaccta cgacaatccc gtgtacgagg gcgaagacgt ctccgtgcag ttctcccacg
1441 acagcatcca caacgccccc gacgaagcct tcatggacat catccgcctc catcgccccg
1501 ccatcgccag taggcgcggg ttggtcagat actcccggat cggccagaga ggcagcatgc
1561 acacccggtc aggcaaacac atcggcgcaa ggatccacta cttctacgac atcagcccca
1621 tcgcccaggc cgccgaggag atcgagatgc accccttggt cgccgcccaa gacgacacct
1681 tcgacatcta cgccgagagc ttcgagcccg acatcaatcc cacacagcat cccgtcacca
1741 acatcagcga cacctacctg accagtaccc ccaacaccgt cacccagccc tggggcaata
1801 caaccgtccc gctctccatc cccaacgatc tgttcctgca aagcgggccc gacatcacct
1861 tcccgaccgc cccaatgggc accccattca gccccgtgac ccccgccctg cccaccgggc
1921 ccgtcttcat caccggcagc ggcttctacc tccaccccgc ttggtacttc gcccgcaaga
1981 ggcgcaagag gatcccactg ttcttctcgg acgtcgccgc atgagatcaa gcttatcgat
2041 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
2101 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
2161 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
2221 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
2281 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
2341 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
2401 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
2461 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
2521 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
2581 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt
2641 cggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
2701 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
2761 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
2821 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
2881 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
2941 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
3001 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
3061 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
3121 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
3181 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
3241 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
3301 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
3361 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
3421 cagcaggcag aagtatgcaa agcatgcaga attctatcaa atatttaaag aaaaaaaaat
3481 tgtatcaact ttctacaatc tctttcagaa gacagaagca gagggaatac ttcctaaatc
3541 attcaactag gccagcatta ccttaatacc ggaactagaa aatgacatta caagaaaaga
3601 aaacaacaga ccaatatctc tcatgaacaa agatacaaac attttcaaca aaatattagc
3661 aaaaagaatc caagaatgta tcaaaaaata tacaccacaa ccaagtagaa tttattccag
3721 atatgtaagg gtggttcaac gtttgaaaat caattaacgt aatttgtccc atcaacaggt
3781 taaagaagaa aatcacatgg tcatattgat agacacagaa aaagcatttg acaaaattta
3841 acacccattc atgatgcaat ctctcagtaa actaggaata gaggaaaact tcctcagctt
3901 gaatgtacct tcctctcaat tttgctatga acctgaaact cctcttaaaa aataaagttt
3961 ttcatttaaa aagaaaacaa aaaacatgga ggagcgttga tgtatctcat tttagaccaa
4021 tcagctatgg atagttaggc gacagcacag atagctgctg tacttctgtt tctggcaatg
4081 ttccagacta catttaaaaa atttttaatt atagacttgt acttaatgtt caagaaaaat
4141 atgaaaatgg ctttgccgtg ttaatgctac tcttttttaa aaaaaactaa agttcaaact
4201 ttatttatat ttcattagtt ttttagctac tgttcttttt ctgttctggg atctcattca
4261 gaatgccaca ttacatataa ttctcatgtc tccttgggtt cctcttagtt ttgacagttc
4321 ctcagacttt tcttattttt gatgaccttg acagttttga ggagtactgg ttagatatag
4381 ggtaatggtt tttaaagtat atttgtcatg atttatactg gggtaagggt ttggggagga
4441 agcccatggg gtaaagtact gttctcatca catcatatca aggttatata ccatcaatat
4501 tgccacagat gttacttagc cttttaatat ttctctaatt tagtgtatat gcaatgatag
4561 ttctctgatt tctgagattg agtttctcat gtgtaatgat tatttagagt ttctctttca
4621 tctgttcaaa tttttgtcta gttttatttt ttactgattt gtaagacttc tttttataat
4681 ctgcatatta caattctctt tactggggtg ttgcaaatat tttctgtcat tctatggcct
4741 gacttttctt aatggttttt taattttaaa aataagtctt aatattcatg caatctaatt
4801 aacaatcttt tctttgtggt taggactttg agtcataaga aatttttctc tacactgaag
4861 tcatgatggc atgcttctat attattttct aaaagattta aagttttgcc ttctccattt
4921 agacttataa ttcactggaa tttttttgtg tgtatggtat gacatatggg ttccctttta
4981 ttttttacat ataaatatat ttccctgttt ttctaaaaaa gaaaaagatc atcattttcc
5041 cattgtaaaa tgccatattt ttttcatagg tcacttacat atatcaatgg gtctgtttct
5101 gagctctact ctattttatc agcctcactg tctatcccca cacatctcat gctttgctct
5161 aaatcttgat atttagtgga acattctttc ccattttgtt ctacaagaat atttttgtta
5221 ttgtcttttg ggcttctata tacattttag aatgaggttg gcaagttaac aaacagcttt
5281 tttggggtga acatattgac tacaaattta tgtggaaaga aagtaccaag ttgaccagtg
5341 ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc
5401 tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga
5461 ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt
5521 gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc
5581 gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg
5641 ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg
5701 tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
5761 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
5821 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
5881 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
5941 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
6001 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
6061 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
6121 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
6181 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
6241 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
6301 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
6361 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
6421 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
6481 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
6541 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
6601 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
6661 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
6721 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
6781 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
6841 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
6901 cttgatccgg caaacaaacc accgctggta gcggtttttt tgtttgcaag cagcagatta
6961 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
7021 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
7081 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
7141 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
7201 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
7261 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
7321 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
7381 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
7441 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
7501 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
7561 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
7621 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
7681 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
7741 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
7801 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
7861 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
7921 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
7981 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
8041 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
8101 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
8161 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
8221 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
8281 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
8341 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
8401 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
8461 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
8521 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
8581 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
8641 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
8701 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
8761 catgatgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
8821 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
8881 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgagcg gtaggcgtgt
8941 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtaagcttc gaggggctcg
9001 catctctcct tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg
9061 cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt
9121 aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag
9181 ccggctctcc acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc
9241 tgttctgcgc cgttacagat ccaagccacc atgtggcgcc catctgattc caccgtgtac
9301 gtcccgccac ccaatcccgt cagcaaggtc gtcgcaaccg acgcctacgt caccaggaca
9361 aacatcttct accacgcatc atccagccgc ctgttggccg tcggccaccc ctacttcagt
9421 atcaagagag ccaacaagac cgtcgtcccc aaggtcagcg gctaccagta tcgcgtgttc
9481 aaagtcgtcc tgcccgaccc caacaagttc gccctgcccg atagcagctt gttcgaccca
9541 accacccaga ggctcgtgtg ggcctgtacc gggctggaag tcgggagagg ccaacccctg
9601 ggcgtcggcg tgtccggcca cccgttcttg aacaagtacg acgacgtcga gaacagcggc
9661 tccggcggca atccaggcca ggacaatcgc gtcaacgtgg gcatggacta caagcagacc
9721 cagctgtgta tggtcggctg cgcaccaccc ctcggggaac actggggcaa gggcaagcaa
9781 tgcaccaaca ccccagtgca ggccggcgat tgtcccccac tggagttgat cacatccgtc
9841 atccaggacg gggacatggt cgataccggg ttcggcgcca tgaacttcgc cgacctccaa
9901 acgaacaaga gcgacgtccc catcgatatc tgcgggacca cctgcaagta ccccgactac
9961 ctgcaaatgg ccgccgatcc ctacggcgac cgcctgttct tcttcttgag aaaagagcag
10021 atgttcgcac gccacttctt caatcgcgcc ggggaagtcg gcgagcccgt ccccgacacc
10081 ctgatcatca aaggctccgg caacaggacc agcgtgggct ccagcatcta cgtcaataca
10141 ccgtctggga gcctcgtcag tagcgaagcc cagctcttca acaaacccta ctggttgcag
10201 aaggcacaag gccacaacaa cggcatctgc tggggcaacc agctcttcgt caccgtcgtg
10261 gacacaacca ggtccacaaa catgaccctg tgcgccagtg tcaccaccag cagtacctac
10321 acgaacagcg actacaagga gtacatgagg cacgtcgagg aatacgacct gcagttcatc
10381 ttccaactgt gctcaatcac cctgtccgcc gaagtcatgg catacatcca taccatgaac
10441 ccaagcgtcc tggaggattg gaacttcggc ctgagccccc cacccaacgg caccctggag
10501 gacacatacc gctacgtcca aagccaggca atcacatgcc agaagccaac ccccgagaaa
10561 gagaagcccg acccatacaa gaacttgagc ttctgggaag tcaacctgaa ggagaagttc
10621 agctccgagc tcgaccagta cccactcggc aggaagttcc tgctgcaatc cggctaccgc
10681 ggcagaagta gcatccgcac aggcgtcaag aggcccgccg tcagcaaggc aagcgccgca
10741 cccaagcgca aaagggcaaa gaccaagcgc tgagct
//
