| 质粒名称: | pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 60904 |
| 存储实验室: | Ron Vale |
|
载体骨架基本信息 |
|
| 载体名称: | pHR |
| 载体抗性: | 氨苄霉素 |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | scFv-GCN4 |
| 插入大小: | 828 bp |
| 材料和方法部分: | pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS was a gift from Ron Vale (Addgene plasmid # 60904) |
| 参考文献部分: | A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging. Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD. Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039. 10.1016/j.cell.2014.09.039 PubMed 25307933 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS 10074 bp DNA SYN
DEFINITION pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..10074
/organism="pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS"
/mol_type="other DNA"
misc_feature complement(120..137)
/label="Sp6_primer"
gene 375..638
/label="tet (300 - 563)"
/gene="tet (300 - 563)"
misc_feature complement(427..446)
/label="pBRrevBam_primer"
misc_feature 780..802
/label="pGEX_3_primer"
promoter 961..989
/label="AmpR_promoter"
gene 1031..1891
/label="Ampicillin"
/gene="Ampicillin"
CDS 1031..1891
/label="ORF frame 2"
rep_origin 2046..2665
/label="pBR322_origin"
misc_feature complement(2891..2911)
/label="pBABE_3_primer"
misc_feature complement(2897..3350)
/label="SV40_enhancer"
promoter 2909..3177
/label="SV40_promoter"
rep_origin 3076..3153
/label="SV40_origin"
misc_feature 3138..3157
/label="SV40pro_F_primer"
misc_feature 4358..4373
/label="SV40_int"
misc_feature 4379..4426
/label="SV40_3_splice"
CDS 4503..4976
/label="ORF frame 3"
misc_feature 4554..4574
/label="SV40 NLS"
misc_feature 4560..4574
/label="NLS"
terminator 5002..5133
/label="SV40_PA_terminator"
misc_feature 5090..5109
/label="EBV_rev_primer"
misc_feature 5755..5935
/label="HIV-1_5_LTR"
misc_feature 5755..5935
/label="truncHIV-1_3_LTR"
misc_feature 6046..6090
/label="HIV-1_psi_pack"
CDS 6477..7574
/label="ORF frame 3"
misc_feature 6599..6832
/label="RRE"
misc_feature 7350..7365
/label="cPPT"
promoter 7548..7727
/label="SV40_promoter"
rep_origin 7595..7672
/label="SV40_origin"
misc_feature 7657..7676
/label="SV40pro_F_primer"
CDS 7811..9844
/label="ORF frame 2"
misc_feature 8552..8578
/label="HA"
gene 8672..9367
/label="GFP (0 - 695)"
/gene="GFP (0 - 695)"
misc_feature complement(8696..8724)
/label="GFP_R_primer"
misc_feature 8846..8875
/label="Y66 (EGFP)"
misc_feature 9319..9339
/label="GFP_F_primer"
misc_feature 9918..9933
/label="cPPT"
misc_feature 9918..9939
/label="U3PPT"
misc_feature 9935..9987
/label="delta_U3"
misc_feature 9988..94
/label="HIV-1_5_LTR"
misc_feature 9988..94
/label="truncHIV-1_3_LTR"
ORIGIN
1 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
61 agaccctttt agtcagtgtg gaaaatctct agcagcatct agaattaatt ccgtgtattc
121 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
181 gttctaacga caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc
241 tatcatctct ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt
301 ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta
361 gccagatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg
421 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca
481 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg
541 ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac
601 tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt
661 acaatctagc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
721 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
781 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
841 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
901 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
961 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
1021 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
1081 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
1141 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
1201 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
1261 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
1321 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
1381 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
1441 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
1501 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
1561 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
1621 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
1681 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
1741 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
1801 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
1861 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
1921 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
1981 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
2041 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
2101 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
2161 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
2221 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
2281 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
2341 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
2401 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
2461 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
2521 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
2581 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
2641 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
2701 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
2761 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
2821 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
2881 atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
2941 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
3001 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
3061 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
3121 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
3181 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tggacacaag
3241 acaggcttgc gagatatgtt tgagaatacc actttatccc gcgtcaggga gaggcagtgc
3301 gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc tctataatct
3361 cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg ctgggacact
3421 tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg cacgtaaact
3481 cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg
3541 tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt
3601 atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc
3661 gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac tgcggttgcg
3721 attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt
3781 ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggattga acagccgtgg
3841 gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca acgcctggca
3901 ctgccgggcg ttgttctttt taacttcagg cgggttacaa tagtttccag taagtattct
3961 ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa
4021 acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca
4081 gtcggccctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc gtctgatgtg
4141 gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag
4201 cgatgccgaa cgtaccgacg atgatttata cgatacggtg attggctacc gtggcggcaa
4261 ctggatttat gagtgggccc cggatctttg tgaaggaacc ttacttctgt ggtgtgacat
4321 aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg
4381 tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa
4441 ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag
4501 aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa
4561 agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc
4621 atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag
4681 ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata
4741 acagttataa tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta
4801 ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata
4861 aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta
4921 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
4981 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
5041 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
5101 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat
5161 catcccaaac ttcccacccc ataccctatt accactgcca attacctagt ggtttcattt
5221 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
5281 atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc
5341 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
5401 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
5461 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
5521 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
5581 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
5641 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
5701 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
5761 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
5821 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
5881 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
5941 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
6001 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
6061 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
6121 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
6181 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
6241 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
6301 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
6361 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
6421 aaagtaagac caccgcacag caagcggccg gtgatcttca gacctggacg atatatatga
6481 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
6541 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
6601 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
6661 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
6721 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
6781 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
6841 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
6901 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
6961 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
7021 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
7081 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
7141 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
7201 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
7261 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtATc
7321 gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
7381 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
7441 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttgga
7501 tcgataagct tgatatcgaa ttcaggcagg cagaagtatg caaagcatgc atctcaatta
7561 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
7621 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
7681 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggctacgc
7741 gccagtcctc cgacagactg agtcgcccgg gggggatctg gagctctcga gaattctcAC
7801 GCGTGCCACC ATGGGCCCCG ACATCGTGAT GACCCAGAGC CCCAGCAGCC TGAGCGCCAG
7861 CGTGGGCGAC CGCGTGACCA TCACCTGCCG CAGCAGCACC GGCGCCGTGA CCACCAGCAA
7921 CTACGCCAGC TGGGTGCAGG AGAAGCCCGG CAAGCTGTTC AAGGGCCTGA TCGGCGGCAC
7981 CAACAACCGC GCCCCCGGCG TGCCCAGCCG CTTCAGCGGC AGCCTGATCG GCGACAAGGC
8041 CACCCTGACC ATCAGCAGCC TGCAGCCCGA GGACTTCGCC ACCTACTTCT GCGCCCTGTG
8101 GTACAGCAAC CACTGGGTGT TCGGCCAGGG CACCAAGGTG GAGCTGAAGC GCGGCGGCGG
8161 CGGCAGCGGC GGCGGCGGCA GCGGCGGCGG CGGCAGCAGC GGCGGCGGCA GCGAGGTGAA
8221 GCTGCTGGAG AGCGGCGGCG GCCTGGTGCA GCCCGGCGGC AGCCTGAAGC TGAGCTGCGC
8281 CGTGAGCGGC TTCAGCCTGA CCGACTACGG CGTGAACTGG GTGCGCCAGG CCCCCGGCCG
8341 CGGCCTGGAG TGGATCGGCG TGATCTGGGG CGACGGCATC ACCGACTACA ACAGCGCCCT
8401 GAAGGACCGC TTCATCATCA GCAAGGACAA CGGCAAGAAC ACCGTGTACC TGCAGATGAG
8461 CAAGGTGCGC AGCGACGACA CCGCCCTGTA CTACTGCGTG ACCGGCCTGT TCGACTACTG
8521 GGGCCAGGGC ACCCTGGTGA CCGTGAGCAG CTACCCATAC GATGTTCCAG ATTACGCTGG
8581 TGGAGGCGGA GGTTCTGGGG GAGGAGGTAG TGGCGGTGGT GGTTCAGGAG GCGGCGGAAG
8641 CTTGGATCCA GGTGGAGGTG GAAGCGGTAG CAAAGGAGAA GAACTTTTCA CTGGAGTTGT
8701 CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC AAATTTTCTG TCCGTGGAGA
8761 GGGTGAAGGT GATGCTACAA ACGGAAAACT CACCCTTAAA TTTATTTGCA CTACTGGAAA
8821 ACTACCTGTT CCGTGGCCAA CACTTGTCAC TACTCTGACC TATGGTGTTC AATGCTTTTC
8881 CCGTTATCCG GATCACATGA AACGGCATGA CTTTTTCAAG AGTGCCATGC CCGAAGGTTA
8941 TGTACAGGAA CGCACTATAT CTTTCAAAGA TGACGGGACC TACAAGACGC GTGCTGAAGT
9001 CAAGTTTGAA GGTGATACCC TTGTTAATCG TATCGAGTTA AAGGGTATTG ATTTTAAAGA
9061 AGATGGAAAC ATTCTTGGAC ACAAACTCGA GTACAACTTT AACTCACACA ATGTATACAT
9121 CACGGCAGAC AAACAAAAGA ATGGAATCAA AGCTAACTTC AAAATTCGCC ACAACGTTGA
9181 AGATGGTTCC GTTCAACTAG CAGACCATTA TCAACAAAAT ACTCCAATTG GCGATGGCCC
9241 TGTCCTTTTA CCAGACAACC ATTACCTGTC GACACAATCT GTCCTTTCGA AAGATCCCAA
9301 CGAAAAGCGT GACCACATGG TCCTTCTTGA GTTTGTAACT GCTGCTGGGA TTACACATGG
9361 CATGGATGAG CTCTACAAAG GTGGAGGTCG GACCGGTGGC GGTGGCGGAG GGGATGCTTT
9421 AGACGATTTT GACTTAGATA TGCTTGGTTC AGACGCGTTA GACGACTTCG ACCTAGACAT
9481 GTTAGGCTCA GATGCATTGG ACGACTTCGA TTTAGATATG TTGGGCTCCG ATGCCCTAGA
9541 TGACTTTGAT CTAGATATGC TAGGTAGTGG AGGAGGATCT CGGACCGAAG AGTACAAGCT
9601 TATCCTGAAC GGTAAAACCC TGAAAGGTGA AACCACCACC GAAGCTGTTG ACGCTGCTAC
9661 CGCGGAAAAA GTTTTCAAAC AGTACGCTAA CGACAACGGT GTTGACGGTG AATGGACCTA
9721 CGACGACGCT ACCAAAACCT TCACGGTAAC CGAAGGTGGT GGTAGCGGTG GTGGTACTAG
9781 TCCAAAAACA AGGAGGAGAC CGCGAAGATC ACAACGGAAA AGGCCGCCTA CGCCATGGCC
9841 GTAAgCGGCC GCGACTCTAG AGTCGACcct ttaagaccaa tgacttacaa ggcagctgta
9901 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
9961 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
10021 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gcct
//
