pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS

LOCUS       pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS	10074 bp 	DNA	SYN
DEFINITION  pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..10074
                     /organism="pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS"
                     /mol_type="other DNA"
     misc_feature    complement(120..137)
                     /label="Sp6_primer"
     gene            375..638
                     /label="tet (300 - 563)"
                     /gene="tet (300 - 563)"
     misc_feature    complement(427..446)
                     /label="pBRrevBam_primer"
     misc_feature    780..802
                     /label="pGEX_3_primer"
     promoter        961..989
                     /label="AmpR_promoter"
     gene            1031..1891
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             1031..1891
                     /label="ORF frame 2"
     rep_origin      2046..2665
                     /label="pBR322_origin"
     misc_feature    complement(2891..2911)
                     /label="pBABE_3_primer"
     misc_feature    complement(2897..3350)
                     /label="SV40_enhancer"
     promoter        2909..3177
                     /label="SV40_promoter"
     rep_origin      3076..3153
                     /label="SV40_origin"
     misc_feature    3138..3157
                     /label="SV40pro_F_primer"
     misc_feature    4358..4373
                     /label="SV40_int"
     misc_feature    4379..4426
                     /label="SV40_3_splice"
     CDS             4503..4976
                     /label="ORF frame 3"
     misc_feature    4554..4574
                     /label="SV40 NLS"
     misc_feature    4560..4574
                     /label="NLS"
     terminator      5002..5133
                     /label="SV40_PA_terminator"
     misc_feature    5090..5109
                     /label="EBV_rev_primer"
     misc_feature    5755..5935
                     /label="HIV-1_5_LTR"
     misc_feature    5755..5935
                     /label="truncHIV-1_3_LTR"
     misc_feature    6046..6090
                     /label="HIV-1_psi_pack"
     CDS             6477..7574
                     /label="ORF frame 3"
     misc_feature    6599..6832
                     /label="RRE"
     misc_feature    7350..7365
                     /label="cPPT"
     promoter        7548..7727
                     /label="SV40_promoter"
     rep_origin      7595..7672
                     /label="SV40_origin"
     misc_feature    7657..7676
                     /label="SV40pro_F_primer"
     CDS             7811..9844
                     /label="ORF frame 2"
     misc_feature    8552..8578
                     /label="HA"
     gene            8672..9367
                     /label="GFP (0 - 695)"
                     /gene="GFP (0 - 695)"
     misc_feature    complement(8696..8724)
                     /label="GFP_R_primer"
     misc_feature    8846..8875
                     /label="Y66 (EGFP)"
     misc_feature    9319..9339
                     /label="GFP_F_primer"
     misc_feature    9918..9933
                     /label="cPPT"
     misc_feature    9918..9939
                     /label="U3PPT"
     misc_feature    9935..9987
                     /label="delta_U3"
     misc_feature    9988..94
                     /label="HIV-1_5_LTR"
     misc_feature    9988..94
                     /label="truncHIV-1_3_LTR"
ORIGIN
    1 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
   61 agaccctttt agtcagtgtg gaaaatctct agcagcatct agaattaatt ccgtgtattc
  121 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
  181 gttctaacga caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc
  241 tatcatctct ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt
  301 ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta
  361 gccagatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg
  421 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca
  481 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg
  541 ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac
  601 tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt
  661 acaatctagc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
  721 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
  781 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
  841 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
  901 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
  961 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
 1021 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
 1081 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
 1141 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
 1201 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
 1261 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
 1321 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
 1381 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
 1441 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
 1501 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
 1561 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
 1621 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
 1681 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
 1741 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
 1801 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
 1861 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
 1921 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
 1981 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
 2041 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
 2101 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
 2161 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
 2221 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
 2281 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
 2341 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
 2401 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
 2461 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
 2521 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
 2581 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
 2641 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
 2701 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
 2761 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
 2821 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
 2881 atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
 2941 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
 3001 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
 3061 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
 3121 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
 3181 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tggacacaag
 3241 acaggcttgc gagatatgtt tgagaatacc actttatccc gcgtcaggga gaggcagtgc
 3301 gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc tctataatct
 3361 cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg ctgggacact
 3421 tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg cacgtaaact
 3481 cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg
 3541 tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt
 3601 atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc
 3661 gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac tgcggttgcg
 3721 attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt
 3781 ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggattga acagccgtgg
 3841 gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca acgcctggca
 3901 ctgccgggcg ttgttctttt taacttcagg cgggttacaa tagtttccag taagtattct
 3961 ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa
 4021 acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca
 4081 gtcggccctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc gtctgatgtg
 4141 gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag
 4201 cgatgccgaa cgtaccgacg atgatttata cgatacggtg attggctacc gtggcggcaa
 4261 ctggatttat gagtgggccc cggatctttg tgaaggaacc ttacttctgt ggtgtgacat
 4321 aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg
 4381 tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa
 4441 ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag
 4501 aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa
 4561 agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc
 4621 atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag
 4681 ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata
 4741 acagttataa tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta
 4801 ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata
 4861 aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta
 4921 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
 4981 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
 5041 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
 5101 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat
 5161 catcccaaac ttcccacccc ataccctatt accactgcca attacctagt ggtttcattt
 5221 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
 5281 atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc
 5341 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
 5401 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
 5461 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
 5521 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
 5581 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
 5641 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
 5701 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
 5761 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
 5821 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
 5881 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
 5941 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
 6001 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
 6061 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
 6121 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
 6181 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
 6241 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
 6301 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
 6361 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
 6421 aaagtaagac caccgcacag caagcggccg gtgatcttca gacctggacg atatatatga
 6481 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
 6541 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
 6601 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
 6661 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
 6721 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
 6781 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
 6841 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
 6901 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
 6961 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
 7021 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
 7081 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
 7141 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
 7201 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
 7261 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtATc
 7321 gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
 7381 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
 7441 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttgga
 7501 tcgataagct tgatatcgaa ttcaggcagg cagaagtatg caaagcatgc atctcaatta
 7561 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
 7621 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
 7681 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggctacgc
 7741 gccagtcctc cgacagactg agtcgcccgg gggggatctg gagctctcga gaattctcAC
 7801 GCGTGCCACC ATGGGCCCCG ACATCGTGAT GACCCAGAGC CCCAGCAGCC TGAGCGCCAG
 7861 CGTGGGCGAC CGCGTGACCA TCACCTGCCG CAGCAGCACC GGCGCCGTGA CCACCAGCAA
 7921 CTACGCCAGC TGGGTGCAGG AGAAGCCCGG CAAGCTGTTC AAGGGCCTGA TCGGCGGCAC
 7981 CAACAACCGC GCCCCCGGCG TGCCCAGCCG CTTCAGCGGC AGCCTGATCG GCGACAAGGC
 8041 CACCCTGACC ATCAGCAGCC TGCAGCCCGA GGACTTCGCC ACCTACTTCT GCGCCCTGTG
 8101 GTACAGCAAC CACTGGGTGT TCGGCCAGGG CACCAAGGTG GAGCTGAAGC GCGGCGGCGG
 8161 CGGCAGCGGC GGCGGCGGCA GCGGCGGCGG CGGCAGCAGC GGCGGCGGCA GCGAGGTGAA
 8221 GCTGCTGGAG AGCGGCGGCG GCCTGGTGCA GCCCGGCGGC AGCCTGAAGC TGAGCTGCGC
 8281 CGTGAGCGGC TTCAGCCTGA CCGACTACGG CGTGAACTGG GTGCGCCAGG CCCCCGGCCG
 8341 CGGCCTGGAG TGGATCGGCG TGATCTGGGG CGACGGCATC ACCGACTACA ACAGCGCCCT
 8401 GAAGGACCGC TTCATCATCA GCAAGGACAA CGGCAAGAAC ACCGTGTACC TGCAGATGAG
 8461 CAAGGTGCGC AGCGACGACA CCGCCCTGTA CTACTGCGTG ACCGGCCTGT TCGACTACTG
 8521 GGGCCAGGGC ACCCTGGTGA CCGTGAGCAG CTACCCATAC GATGTTCCAG ATTACGCTGG
 8581 TGGAGGCGGA GGTTCTGGGG GAGGAGGTAG TGGCGGTGGT GGTTCAGGAG GCGGCGGAAG
 8641 CTTGGATCCA GGTGGAGGTG GAAGCGGTAG CAAAGGAGAA GAACTTTTCA CTGGAGTTGT
 8701 CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC AAATTTTCTG TCCGTGGAGA
 8761 GGGTGAAGGT GATGCTACAA ACGGAAAACT CACCCTTAAA TTTATTTGCA CTACTGGAAA
 8821 ACTACCTGTT CCGTGGCCAA CACTTGTCAC TACTCTGACC TATGGTGTTC AATGCTTTTC
 8881 CCGTTATCCG GATCACATGA AACGGCATGA CTTTTTCAAG AGTGCCATGC CCGAAGGTTA
 8941 TGTACAGGAA CGCACTATAT CTTTCAAAGA TGACGGGACC TACAAGACGC GTGCTGAAGT
 9001 CAAGTTTGAA GGTGATACCC TTGTTAATCG TATCGAGTTA AAGGGTATTG ATTTTAAAGA
 9061 AGATGGAAAC ATTCTTGGAC ACAAACTCGA GTACAACTTT AACTCACACA ATGTATACAT
 9121 CACGGCAGAC AAACAAAAGA ATGGAATCAA AGCTAACTTC AAAATTCGCC ACAACGTTGA
 9181 AGATGGTTCC GTTCAACTAG CAGACCATTA TCAACAAAAT ACTCCAATTG GCGATGGCCC
 9241 TGTCCTTTTA CCAGACAACC ATTACCTGTC GACACAATCT GTCCTTTCGA AAGATCCCAA
 9301 CGAAAAGCGT GACCACATGG TCCTTCTTGA GTTTGTAACT GCTGCTGGGA TTACACATGG
 9361 CATGGATGAG CTCTACAAAG GTGGAGGTCG GACCGGTGGC GGTGGCGGAG GGGATGCTTT
 9421 AGACGATTTT GACTTAGATA TGCTTGGTTC AGACGCGTTA GACGACTTCG ACCTAGACAT
 9481 GTTAGGCTCA GATGCATTGG ACGACTTCGA TTTAGATATG TTGGGCTCCG ATGCCCTAGA
 9541 TGACTTTGAT CTAGATATGC TAGGTAGTGG AGGAGGATCT CGGACCGAAG AGTACAAGCT
 9601 TATCCTGAAC GGTAAAACCC TGAAAGGTGA AACCACCACC GAAGCTGTTG ACGCTGCTAC
 9661 CGCGGAAAAA GTTTTCAAAC AGTACGCTAA CGACAACGGT GTTGACGGTG AATGGACCTA
 9721 CGACGACGCT ACCAAAACCT TCACGGTAAC CGAAGGTGGT GGTAGCGGTG GTGGTACTAG
 9781 TCCAAAAACA AGGAGGAGAC CGCGAAGATC ACAACGGAAA AGGCCGCCTA CGCCATGGCC
 9841 GTAAgCGGCC GCGACTCTAG AGTCGACcct ttaagaccaa tgacttacaa ggcagctgta
 9901 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
 9961 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
10021 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gcct

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