pQCXIH

价格:1000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pQCXIH
质粒类型: 哺乳动物细胞双顺反子逆病毒基因表达载体
高拷贝/低拷贝: 低拷贝
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 7780bp
5' 测序引物及序列: pQC-F: ACGCCATCCACGCTGTTTTGACCT
3' 测序引物及序列: pQC-R: AAGCGGCTTCGGCCAGTAACGTTA
载体标签: --
载体抗性: 氨苄
筛选标记: 潮霉素(Hygromycin)
备注: 自失活双顺反子逆病毒表达载体,含有IRES-Hygromycin元件,能够同时表达目的蛋白和潮霉素抗性筛选标记,可以用来构建稳表达细胞系。
产品目录号: 631516
稳定性: 稳表达
组成型: 组成型
病毒/非病毒: 逆病毒

载体质粒图谱和多克隆位点信息

pQCXIH载体图谱



pQCXIH多克隆位点

RNAi-Ready pSIREN-RetroQ载体特征

载体简介

载体序列

LOCUS       pQCXIH   7780 bp    DNA   circular            13-NOV-2009
COMMENT     Created by Clontech Laboratories
COMMENT     Cat. No. 631516
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     LTR             1..728
                     /label=5'\LTR\(CMV/MSV)
     misc_signal     758..1567
                     /label=Extended\packaging\signal
     LTR             4486..4911
                     /label=3'\MMLV\LTR\(del)
     rep_origin      5798..5798
                     /label=pUC\origin
     misc_marker     complement(6558..7418)
                     /label=Amp(R)
     rep_origin      5412..5477
                     /label=SV40\origin
     misc_marker     2905..3939
                     /label=Hyg(R)
     promoter        1601..2132
                     /label=CMV\IE\Promoter
     misc_structure  2292..2881
                     /label=IRES
     promoter        5191..5458
                     /label=SV40\Promoter
BASE COUNT     1802 a      2104 c      2010 g      1864 t 
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc 
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac 
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc 
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc 
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc 
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc 
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat 
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc 
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc 
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta 
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc 
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc 
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca 
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct 
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc 
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga 
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt 
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg 
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga 
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa 
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct 
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct 
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg 
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac 
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt 
     1921 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc 
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc 
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata 
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg 
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa 
     2221 cggacctgca ggaattgatc cgcggccgca ccggtaggcc tcgtacgctt aattaacgga 
     2281 tccggaattc cgcccccccc cccccccccc ctctccctcc ccccccccta acgttactgg 
     2341 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt 
     2401 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc 
     2461 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc 
     2521 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg 
     2581 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc 
     2641 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa 
     2701 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg 
     2761 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa 
     2821 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata 
     2881 agcttgccac aacccgtacc aaagatggat agatccggaa agcctgaact caccgcgacg 
     2941 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg 
     3001 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg 
     3061 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg 
     3121 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat 
     3181 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc 
     3241 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag 
     3301 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat 
     3361 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc 
     3421 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc 
     3481 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc 
     3541 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc 
     3601 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc 
     3661 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt 
     3721 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg 
     3781 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc 
     3841 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga 
     3901 aaccgacgcc ccagcactcg tccgagggca aaggaataga gtagatgccg accgaacaag 
     3961 agctgatttc gagaacgcct cagccagcaa ctcgcgcgag cctagcaagg caaatgcgag 
     4021 agaacggcct tacgcttggt ggcacagttc tcgtccacag ttcgctaagc tcgctcggct 
     4081 gggtcgcggg agggccggtc gcagtgattc aggcccttct ggattgtgtt ggtccccagg 
     4141 gcacgattgt catgcccacg cactcgggtg atctgactga tcccgcagat tggagatcgc 
     4201 cgcccgtgcc tgccgattgg gtgcagatct ctatcgaggg ctgcagcgct gcagaggccg 
     4261 agtgcagaac tgctccaaag ggacctcaag gctttccgag ggacactagg ctgactccat 
     4321 cgagccagtg tagagataag cttatcgatt agtccaattt gttaaagaca ggatatcagt 
     4381 ggtccaggct ctagttttga ctcaacaata tcaccagctg aagcctatag agtacgagcc 
     4441 atagataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac 
     4501 ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata 
     4561 actgagaata gagaagttca gatcaaggtc aggaacagat ggaacagggt cgaccctaga 
     4621 gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga 
     4681 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata 
     4741 aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt 
     4801 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt 
     4861 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat ttgggggctc 
     4921 gtccgggatc gggagacccc tgcccaggga ccaccgaccc accaccggga ggtaagctgg 
     4981 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 
     5041 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 
     5101 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta 
     5161 gatccggctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg 
     5221 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 
     5281 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 
     5341 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 
     5401 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt 
     5461 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttactggctt 
     5521 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg 
     5581 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac 
     5641 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 
     5701 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 
     5761 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 
     5821 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 
     5881 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 
     5941 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 
     6001 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 
     6061 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 
     6121 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 
     6181 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 
     6241 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 
     6301 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 
     6361 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 
     6421 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 
     6481 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 
     6541 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 
     6601 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 
     6661 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 
     6721 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 
     6781 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 
     6841 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg 
     6901 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 
     6961 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 
     7021 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 
     7081 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 
     7141 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc 
     7201 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 
     7261 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 
     7321 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 
     7381 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 
     7441 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 
     7501 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa 
     7561 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt 
     7621 caagaattag cttggccatt gcatacgttg tatccatatc ataatatgta catttatatt 
     7681 ggctcatgtc caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa 
     7741 tcaattacgg ggtcattagt tcatagccca tatatggagt 
//

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