| 质粒名称: | GOMO-GFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 60975 |
| 存储实验室: | Olivier Silvie |
|
载体骨架基本信息 |
|
| 载体名称: | pBluescript |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 2877 bp |
| 完整质粒大小: | 11696 bp |
| 载体修饰: | -- |
| 载体类型: | 疟原虫基因打靶 |
| 筛选标记: | hDHFR-yFCU |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | GFP |
| 物种来源: | Synthetic |
| 插入大小: | 726 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | GOMO-GFP was a gift from Olivier Silvie (Addgene plasmid # 60975 ; http://n2t.net/addgene:60975 ; RRID:Addgene_60975) |
| 参考文献部分: | A rapid and robust selection procedure for generating drug-selectable marker-free recombinant malaria parasites. Manzoni G, Briquet S, Risco-Castillo V, Gaultier C, Topcu S, Ivanescu ML, Franetich JF, Hoareau-Coudert B, Mazier D, Silvie O. Sci Rep. 2014 Apr 23;4:4760. doi: 10.1038/srep04760. 10.1038/srep04760 PubMed 24755823 |
买家导航
GOMOGFP载体基本信息
GOMOGFP载体图谱质粒图谱和多克隆位点信息
GOMOGFP载体简介
GOMOGFP载体序列
LOCUS GOMO-GFP 11695 bp ds-DNA circular SYN 18-DEC-2019
DEFINITION Rapid selection of recombinant marker-free fluorescent rodent
malaria parasites.
ACCESSION .
VERSION .
KEYWORDS GOMO-GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11695)
AUTHORS Manzoni G, Briquet S, Risco-Castillo V, Gaultier C, Topcu S,
Ivanescu ML, Franetich JF, Hoareau-Coudert B, Mazier D, Silvie O
TITLE A rapid and robust selection procedure for generating
drug-selectable marker-free recombinant malaria parasites.
JOURNAL Sci Rep. 2014 Apr 23;4:4760. doi: 10.1038/srep04760.
PUBMED 24755823
REFERENCE 2 (bases 1 to 11695)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 18, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11695
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(987..1697)
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(1265..1284)
/label=mCherry-F
/note="mCherry, forward primer"
primer_bind 1534..1552
/label=mCherry-R
/note="mCherry, reverse primer"
CDS complement(6196..6909)
/codon_start=1
/product="Aequoria victoria green fluorescent protein"
/label=GFP
/translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
ICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
VTAAGITHGMDELYK"
primer_bind complement(6239..6259)
/label=GFP-F
/note="GFP, forward primer. Does NOT anneal to EGFP"
primer_bind 6854..6882
/label=GFP-R
/note="GFP, reverse primer. Does NOT anneal to EGFP"
primer_bind complement(8855..8875)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(8855..8873)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(8894..8910)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8894..8910)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(8907..8929)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 8918..8934
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8942..8972)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8987..9008
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(9125..9142)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(9296..9884)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(9376..9395)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(10055..10915)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10678..10697
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10916..11020)
/gene="bla"
/label=AmpR promoter
rep_origin complement(11046..11501)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(11183..11204)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 11395..11414
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 11628..11650
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 11642..11659
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 11643..11659
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 11669..11688
/label=T7
/note="T7 promoter, forward primer"
promoter 11669..11687
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 ggtaccactc tcgagtgaaa ttaataaaat aaaatacata tccctcacaa attatatata
61 tatatatctc atatttataa gttattatta taaccatcta tgataaattt tcaaaataat
121 tttttttttt ttttgtagga tgaatatttc taccctgaag aagaaaagtc cgatgatgtt
181 gtggacataa agaattgagc tgtctcaaaa aatgagaaaa caaacaaaca aacaaaatac
241 cattatgcgc atatcctctg ctcaatatca atttttcttt atgccagttt tgtttcgaca
301 ctatttgagc aacaccttcc ctttactatc tactactgtt acaatgttat tttattcacc
361 tttttacaat tttatcaaaa tttttttgta tgcatataaa ttatacattg taaagccaaa
421 gtgtagataa agaacacctt gtttattttt ccccaatttt tttttttttt accatttttt
481 tattaacctt gctcatttac ctgctaatac gattgctata tgatatcgaa attgaaggaa
541 aaaacatcat ttgtgtttat atgttttttt ttaatttttc aacttttttt atttaactat
601 gctaatcata ttataaacat tttaaaaatc aagtccaact atttatgaat cattgaagag
661 acaacataat tataacagta aaaataataa taataataat aattttgtct tattgtttca
721 aacatattat gttccataaa tatctaaatt tttaatatgg tcataaattc ataggattta
781 atttatataa aatatggcta ttcatactag ccattttatg tgtgtaaagt ttttttttct
841 gaacaaaaaa taaaatgttc ataattttag ctatttacat gcatgtgcat gcacaaaaaa
901 aaatatgcac acaacataca catttttaca gttataaata caatcaattg gtataaatat
961 ataaataaga aaaacgaaca gtcgagttac ttgtacagct cgtccatgcc gccggtggag
1021 tggcggccct cggcgcgttc gtactgttcc acgatggtgt agtcctcgtt gtgggaggtg
1081 atgtccaact tgatgttgac gttgtaggcg ccgggcagct gcacgggctt cttggccttg
1141 taggtggtct tgacctcagc gtcgtagtgg ccgccgtcct tcagcttcag cctctgcttg
1201 atctcgccct tcagggcgcc gtcctcgggg tacatccgct cggaggaggc ctcccagccc
1261 atggtcttct tctgcattac ggggccgtcg gaggggaagt tggtgccgcg cagcttcacc
1321 ttgtagatga actcgccgtc ctgcagggag gagtcctggg tcacggtcac cacgccgccg
1381 tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg ggaaggacag cttcaagtag
1441 tcggggatgt cggcggggtg cttcacgtag gccttggagc cgtacatgaa ctgaggggac
1501 aggatgtccc aggcgaaggg cagggggcca cccttggtca ccttcagctt ggcggtctgg
1561 gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg gccgttcacg
1621 gagccctcca tgtgcacctt gaagcgcatg aactccttga tgatggccat gttatcctcc
1681 tcgcccttgc tcaccatttt cctaggtata aaatttttat ttatttataa gcaaatatat
1741 atttttatat atttatacac aaagtatata tattttagga aatattaaaa taaatttaaa
1801 ttttgaaggc aaatgaaaaa attacgtttt ttgtttcaca acaattatat ttattttttg
1861 aattaattta catattattt tatattaaaa ttataaaata aaaaaaacat tacatatatt
1921 aattatatta ttatatgagg atttttttat aacatagaaa tataaataat tacgactagt
1981 taataaaggg cactttaatt tttatgcata attagcttat atttagtgca tatttatttt
2041 tatacatatt tttatatatt ttttgattta catggcgttt tatgtttata tgtataacat
2101 tatatgcata gtattttttt gaaaatttaa tggtatttca gtggatgaaa atattactgg
2161 tgctttgagg ggtgagcatt taaagcacaa tatctaggat actacaaaaa atacttaaag
2221 tatgaatatt aaattgtaaa cttaagcata aagagctcga aaagaattaa gcttaactca
2281 tctacaagca tcaaattaaa tgttttatgc agtacctttt tttaaataat ccattttatt
2341 tttttatact ccgaggattt tacacctgca ctgacactac aaatatagta atattttatc
2401 cctacattat atgcatttta taatattttt atttatttta ataatttata cacataagta
2461 catagtatag agttgaaaaa aaaatacgtg tatttctaaa ataaaatgaa ataaatatta
2521 tgttattata ttgaatacaa taaacatttt ctccaaatta aaatttaata ttatataaca
2581 aaaaaaaata caaaacacca attaaaaata ataataattt tatccttatt ttttaataac
2641 tgccattttt aaagtatata ataaaaaata aattaaattt taaaaatatt tcaaaatata
2701 aaataaatat ataagaatat atatttgctt ataaataaat aaaaatttta tagtcgacaa
2761 aatggttggt tcgctaaact gcatcgtcgc tgtgtcccag aacatgggca tcggcaagaa
2821 cggggacctg ccctggccac cgctcaggaa cgaatttaga tatttccaga gaatgaccac
2881 aacctcttca gtagaaggta aacagaatct ggtgattatg ggtaagaaga cctggttctc
2941 cattcctgag aagaatcgac ctttaaaggg tagaattaat ttagttctca gcagagaact
3001 caaggaacct ccacaaggag ctcattttct ttccagaagt ctagatgatg ccttaaaact
3061 tactgaacaa ccagaattag caaataaagt agacatggtc tggatagttg gtggcagttc
3121 tgtttataag gaagccatga atcacccagg ccatcttaaa ctatttgtga caaggatcat
3181 gcaagacttt gaaagtgaca cgttttttcc agaaattgat ttggagaaat ataaacttct
3241 gccagaatac ccaggtgttc tctctgatgt ccaggaggag aaaggcatta agtacaaatt
3301 tgaagtatat gagaagaatg atgcaagcgg aggaggtgga tctggtggag gtggaagtgc
3361 aagcgtgaca gggggaatgg ctagcaagtg ggatcagaag ggtatggaca ttgcctatga
3421 ggaggcggcc ttaggttaca aggagggtgg tgttcctatt ggcggatgtc ttatcaataa
3481 caaagacgga agtgttctcg gtcgtggtca caacatgaga tttcaaaagg gatccgccac
3541 actacatggt gagatctcca ctttggaaaa ctgtgggaga ttagagggca aagtgtacaa
3601 agataccact ttgtatacga cgctgtctcc atgcgacatg tgtacaggtg ccatcatcat
3661 gtatggtatt ccacgctgtg ttgtcggtga gaacgttaat ttcaaaagta agggcgagaa
3721 atatttacaa actagaggtc acgaggttgt tgttgttgac gatgagaggt gtaaaaagat
3781 catgaaacaa tttatcgatg aaagacctca ggattggttt gaagatattg gtgaggcttc
3841 ggaaccattt aagaacgtct acttgctacc tcaaacaaac caattgctgg gtttgtacac
3901 catcatcaga aataagaata caactagacc tgatttcatt ttctactccg atagaatcat
3961 cagattgttg gttgaagaag gtttgaacca tctacctgtg caaaagcaaa ttgtggaaac
4021 tgacaccaac gaaaacttcg aaggtgtctc attcatgggt aaaatctgtg gtgtttccat
4081 tgtcagagct ggtgaatcga tggagcaagg attaagagac tgttgtaggt ctgtgcgtat
4141 cggtaaaatt ttaattcaaa gggacgagga gactgcttta ccaaagttat tctacgaaaa
4201 attaccagag gatatatctg aaaggtatgt cttcctatta gacccaatgc tggccaccgg
4261 tggtagtgct atcatggcta cagaagtctt gattaagaga ggtgttaagc cagagagaat
4321 ttacttctta aacctaatct gtagtaagga agggattgaa aaataccatg ccgccttccc
4381 agaggtcaga attgttactg gtgccctcga cagaggtcta gatgaaaaca agtatctagt
4441 tccagggttg ggtgactttg gtgacagata ctactgtgtt taagaattga ttattgttct
4501 gtacttcttt tgtgaatagt atttttacat atgtactgtt aattttaaaa tgtggaatgc
4561 cacgaacata ttttttcgaa caaaatatat attttttgtg tcgaaataca taaatttgtg
4621 tacctccttt ttgttgtata tatgaaaaag gaataaagtg aatggagacg aattactcta
4681 gaccctacaa cttgtatatc actactttat aatactataa tattattatt aacattaaac
4741 tatagaagaa atatcaacaa tacttatgta tttgttcaaa ctttatcagt atagtttggt
4801 aatcgcatat catatatttg ttatgtgtaa tattttatga atatgcgtct ttttttttaa
4861 ctataaataa ttcaaaataa aataacataa ttaacatttg caagcataat taagatgatt
4921 actttcacat ttcatccaaa gtggaaaatg accaccacat ttgatcgaag cttttaccac
4981 ttttgatgca ttagctctga aatgtataca agctttcatt tgttaatgtt acaaagggaa
5041 gtttttattt gggggcacat taaaagtgaa aaaattaaaa gttaaaaaag ttaaaaaatg
5101 atcggtgtag ttggtcatat agacatgttg ttatgcaagt ctttcaaaac gttcctcata
5161 aaataaaagg agggatctag agagctttca attctccaaa cggtattatt ggtatttgga
5221 attgtgaaat taataaaata aaatacatat ccctcacaaa ttatatatat atatatctca
5281 tatttataag ttattattat aaccatctat gataaatttt caaaataatt tttttttttt
5341 tttgtaggat gaatatttct accctgaaga agaaaagtcc gatgatgttg tggacataaa
5401 gaattgagct gtctcaaaaa atgagaaaac aaacaaacaa acaaaatacc attatgcgca
5461 tatcctctgc tcaatatcaa tttttcttta tgccagtttt gtttcgacac tatttgagca
5521 acaccttccc tttactatct actactgtta caatgttatt ttattcacct ttttacaatt
5581 ttatcaaaat ttttttgtat gcatataaat tatacattgt aaagccaaag tgtagataaa
5641 gaacaccttg tttatttttc cccaattttt ttttttttac cattttttta ttaaccttgc
5701 tcatttacct gctaatacga ttgctatatg atatcgaaat tgaaggaaaa aacatcattt
5761 gtgtttatat gttttttttt aatttttcaa ctttttttat ttaactatgc taatcatatt
5821 ataaacattt taaaaatcaa gtccaactat ttatgaatca ttgaagagac aacataatta
5881 taacagtaaa aataataata ataataataa ttttgtctta ttgtttcaaa catattatgt
5941 tccataaata tctaaatttt taatatggtc ataaattcat aggatttaat ttatataaaa
6001 tatggctatt catactagcc attttatgtg tgtaaagttt tttttttctg aacaaaaaat
6061 aaaatgttca taattttagc tatttacatg catgtgcatg cacaaaaaaa aatatgcaca
6121 caacatacac atttttacag ttataaatac aatcaattgg tataaatata taaataagaa
6181 aaacgaacag aattcttatt tgtatagttc atccatgcca tgtgtaatcc cagcagctgt
6241 tacaaactca agaaggacca tgtggtctct cttttcgttg ggatctttcg aaagggcaga
6301 ttgtgtggac aggtaatggt tgtctggtaa aaggacaggg ccatcgccaa ttggagtatt
6361 ttgttgataa tggtctgcta gttgaacgct tccatcttca atgttgtgtc taattttgaa
6421 gttaactttg attccattct tttgtttgtc tgccatgatg tatacattgt gtgagttata
6481 gttgtattcc aatttgtgtc caagaatgtt tccatcttct ttaaaatcaa taccttttaa
6541 ctcgattcta ttaacaaggg tatcaccttc aaacttgact tcagcacgtg tcttgtagtt
6601 cccgtcatct ttgaaaaata tagttctttc ctgtacataa ccttcgggca tggcactctt
6661 gaaaaagtca tgctgtttca tatgatctgg gtatctcgca aagcattgaa gaccatacgc
6721 gaaagtagtg acaagtgttg gccatggaac aggtagtttt ccagtagtgc aaataaattt
6781 aagggtaagt tttccgtatg ttgcatcacc ttcaccctct ccactgacag aaaatttgtg
6841 cccattaaca tcaccatcta attcaacaag aattgggaca actccagtga aaagttcttc
6901 tcctttacta gccttaagca ttttcctagg gtaattgtaa tttattggga taataatgtt
6961 ggtaattatt ttatttaatt aatttgctac aagcatatat atatatattt aatgtgctta
7021 tggcaaaata gtgtaaaatg caaaaatgta tataatacaa agaaatatgt tttgggcttt
7081 aattttttaa aaaaacatat acttataatt taagcaataa atatatatct atatatttct
7141 ttaatttgac cgttaaaaat aaattggaat gaatatttaa aaaaaataaa aagtatgtta
7201 atttttttaa tgttatataa aaagtattct tatattgtta ttatataata ataacaagct
7261 ctataacaat tgggtaaaat ttttattaaa aaaggagaat gcaattaaaa tatatacata
7321 taatataaaa aaattaatta taaatatatt ttatataaaa taagaaatat agtgtattgt
7381 aaattattaa aattaactac gatatataat aggaaataac actatttatt ttttctctaa
7441 agaaaaatat ataatattat atatatataa tcaaaagggg gtatatatta tggtatatat
7501 atgtaaataa tatttataat tatttttctt aatatatata ttctttttta aatatttttt
7561 tgcatgaaaa ttaatttatt tgagttaaca ttaatttatt tgatatcctt tttataagca
7621 tatatacttc ttaatttatg ggactaaagt tcgtactttt tattttttta tttttattat
7681 tagctataaa aaaaacaaaa tacggggcca tttattagcc ccttagttaa agcagctatt
7741 atatatacct atatatgggg tcccctttat ataatatttt ttttttattg gcaaggatag
7801 tcataatata gtatgcataa tatatataca tatttatatg cttcgcaaat gcaaaaaatg
7861 ttttgtattc caccttttta tgggttttat attttttcaa atttccgaaa gtgcaattat
7921 ttgcaaaaat atgaactgaa aaagagcgca aatgcattgc cccacgcaaa atgaatgtat
7981 tgtaaaataa caaatatata taatgctaat ataatgctat ataataatat attataaaca
8041 tagtatgcac cctttgaggg cttggggggg gcccctccct attttatttg gcaattataa
8101 tttaaagcca tatatttttc atattatatt atgtacaatt ccgcaatttg ttgtacataa
8161 aataggcaga atataaaatt tattttatat aacaaattca atgaacttct aatatgactc
8221 ataaaattat tttcttagca aaaattttgc acaaacaata ttaaaagttt ttaagctcac
8281 caacaaaata ttacgtatat tttccctcaa attaattaaa taaatgaaca aataagcaaa
8341 taaataaatc atgttatgta ttatccctat agttattctt atatatatat atatatatat
8401 aatatatttc ctttttttat atacattaaa aaaatacacc tttttaatta taaatgaaaa
8461 aaaagtgaaa acaacataaa gttatttgct cgatcgaaca ttatggcaat ttttcaaata
8521 tgataattat attaaaatat gttttgaaaa tttaaagaca ttacattaaa tttaaaaaaa
8581 tatattatat acattttttg aaaaaagact aaaatgtaat aggacattct ctcgataatt
8641 taaactcagt taatttgttt attatgatat ttttgaaatt tacatcattt tatatatttt
8701 tttattttat atttttcatc actctttttt ttgttctttt ataataattg agtctttagt
8761 aacgaattgc catttgaaaa ctgagggaat atacactgta gatcccgtac ggctgagggc
8821 ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct
8881 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
8941 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
9001 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
9061 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
9121 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
9181 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
9241 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
9301 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
9361 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
9421 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
9481 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
9541 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
9601 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
9661 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
9721 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
9781 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
9841 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
9901 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
9961 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
10021 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
10081 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
10141 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
10201 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
10261 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
10321 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
10381 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
10441 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
10501 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
10561 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
10621 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
10681 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
10741 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
10801 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
10861 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
10921 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
10981 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
11041 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
11101 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
11161 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
11221 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
11281 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
11341 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
11401 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
11461 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
11521 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
11581 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
11641 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattg
//
