pRL-CMV

价格:1000元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Promega
载体名称: pRL-CMV
质粒类型: 哺乳细胞表达;启动子增强子报告系统
高拷贝/低拷贝: --
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 4079bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素(Ampicillin)
筛选标记: --
备注: pRL-CMV是海肾荧光素酶报告载体。
产品目录号: E2261
稳定性: 瞬表达
组成型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pRL-CMV载体图谱
pRL-CMV载体特征
pRL-CMV载体图谱2

载体简介

载体序列

LOCUS       pRL-CMV	4079 bp 	DNA	SYN
DEFINITION  pRL-CMV
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="pRL-CMV"
                     /mol_type="other DNA"
     promoter        169..721
                     /label="CMV_immearly_promoter"
     misc_feature    224..511
                     /label="CAG_enhancer"
     promoter        667..748
                     /label="CMV_promoter"
     misc_feature    678..698
                     /label="CMV_fwd_primer"
     misc_feature    722..741
                     /label="pCEP_fwd_primer"
     misc_feature    724..748
                     /label="LNCX_primer"
     misc_feature    863..995
                     /label="chim_int"
     promoter        1040..1058
                     /label="T7_promoter"
     CDS             1068..2003
                     /label="ORF frame 3"

     terminator      complement(2031..2150)
                     /label="SV40_PA_terminator"

     misc_feature    complement(2045..2064)
                     /label="EBV_rev_primer"

     promoter        2323..2351
                     /label="AmpR_promoter"

     gene            2393..3253
                     /label="Ampicillin"
                     /gene="Ampicillin"

     CDS             2393..3253
                     /label="ORF frame 2"

     rep_origin      3408..4027
                     /label="pBR322_origin"

ORIGIN
    1 AGATCTTCAA TATTGGCCAT TAGCCATATT ATTCATTGGT TATATAGCAT AAATCAATAT
   61 TGGCTATTGG CCATTGCATA CGTTGTATCT ATATCATAAT ATGTACATTT ATATTGGCTC
  121 ATGTCCAATA TGACCGCCAT GTTGGCATTG ATTATTGACT AGTTATTAAT AGTAATCAAT
  181 TACGGGGTCA TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA
  241 TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT
  301 TCCCATAGTA ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA
  361 AACTGCCCAC TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTCCGCCCC CTATTGACGT
  421 CAATGACGGT AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAC GGGACTTTCC
  481 TACTTGGCAG TACATCTACG TATTAGTCAT CGCTATTACC ATGGTGATGC GGTTTTGGCA
  541 GTACACCAAT GGGCGTGGAT AGCGGTTTGA CTCACGGGGA TTTCCAAGTC TCCACCCCAT
  601 TGACGTCAAT GGGAGTTTGT TTTGGCACCA AAATCAACGG GACTTTCCAA AATGTCGTAA
  661 TAACCCCGCC CCGTTGACGC AAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
  721 CAGAGCTCGT TTAGTGAACC GTCAGATCAC TAGAAGCTTT ATTGCGGTAG TTTATCACAG
  781 TTAAATTGCT AACGCAGTCA GTGCTTCTGA CACAACAGTC TCGAACTTAA GCTGCAGAAG
  841 TTGGTCGTGA GGCACTGGGC AGGTAAGTAT CAAGGTTACA AGACAGGTTT AAGGAGACCA
  901 ATAGAAACTG GGCTTGTCGA GACAGAGAAG ACTCTTGCGT TTCTGATAGG CACCTATTGG
  961 TCTTACTGAC ATCCACTTTG CCTTTCTCTC CACAGGTGTC CACTCCCAGT TCAATTACAG
 1021 CTCTTAAGGC TAGAGTACTT AATACGACTC ACTATAGGCT AGCCACCATG ACTTCGAAAG
 1081 TTTATGATCC AGAACAAAGG AAACGGATGA TAACTGGTCC GCAGTGGTGG GCCAGATGTA
 1141 AACAAATGAA TGTTCTTGAT TCATTTATTA ATTATTATGA TTCAGAAAAA CATGCAGAAA
 1201 ATGCTGTTAT TTTTTTACAT GGTAACGCGG CCTCTTCTTA TTTATGGCGA CATGTTGTGC
 1261 CACATATTGA GCCAGTAGCG CGGTGTATTA TACCAGACCT TATTGGTATG GGCAAATCAG
 1321 GCAAATCTGG TAATGGTTCT TATAGGTTAC TTGATCATTA CAAATATCTT ACTGCATGGT
 1381 TTGAACTTCT TAATTTACCA AAGAAGATCA TTTTTGTCGG CCATGATTGG GGTGCTTGTT
 1441 TGGCATTTCA TTATAGCTAT GAGCATCAAG ATAAGATCAA AGCAATAGTT CACGCTGAAA
 1501 GTGTAGTAGA TGTGATTGAA TCATGGGATG AATGGCCTGA TATTGAAGAA GATATTGCGT
 1561 TGATCAAATC TGAAGAAGGA GAAAAAATGG TTTTGGAGAA TAACTTCTTC GTGGAAACCA
 1621 TGTTGCCATC AAAAATCATG AGAAAGTTAG AACCAGAAGA ATTTGCAGCA TATCTTGAAC
 1681 CATTCAAAGA GAAAGGTGAA GTTCGTCGTC CAACATTATC ATGGCCTCGT GAAATCCCGT
 1741 TAGTAAAAGG TGGTAAACCT GACGTTGTAC AAATTGTTAG GAATTATAAT GCTTATCTAC
 1801 GTGCAAGTGA TGATTTACCA AAAATGTTTA TTGAATCGGA CCCAGGATTC TTTTCCAATG
 1861 CTATTGTTGA AGGTGCCAAG AAGTTTCCTA ATACTGAATT TGTCAAAGTA AAAGGTCTTC
 1921 ATTTTTCGCA AGAAGATGCA CCTGATGAAA TGGGAAAATA TATCAAATCG TTCGTTGAGC
 1981 GAGTTCTCAA AAATGAACAA TAATTCTAGA GCGGCCGCTT CGAGCAGACA TGATAAGATA
 2041 CATTGATGAG TTTGGACAAA CCACAACTAG AATGCAGTGA AAAAAATGCT TTATTTGTGA
 2101 AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTAACAA
 2161 CAACAATTGC ATTCATTTTA TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG
 2221 CAAGTAAAAC CTCTACAAAT GTGGTAAAAT CGATAAGGAT CCAGGTGGCA CTTTTCGGGG
 2281 AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT
 2341 CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT
 2401 TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC
 2461 TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG
 2521 TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG
 2581 TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA
 2641 CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA
 2701 CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC
 2761 TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC
 2821 GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG
 2881 GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC
 2941 AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA
 3001 ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT
 3061 TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT
 3121 CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG
 3181 GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT
 3241 TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT
 3301 TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT
 3361 CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC
 3421 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT
 3481 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG
 3541 CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA
 3601 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC
 3661 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA
 3721 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC
 3781 GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA
 3841 AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG
 3901 GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG
 3961 ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG
 4021 CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGGCTCGAC

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