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pSUPERIOR.puro载体质粒基本信息
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pSUPERIOR.puro质粒载体简介
pSUPERIOR.puro质粒序列载体序列
LOCUS pSUPERIOR.puro 4354 bp DNA circular SYN 11-JUL-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4354)
AUTHORS Biofeng
TITLE Direct Submission
JOURNAL Exported Jul 11, 2023 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4354
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(30..458)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 600..616
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 626..644
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 677..693
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
CDS complement(756..1355)
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
promoter complement(1380..1879)
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
protein_bind 2090..2108
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
primer_bind complement(2120..2136)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(2166..2184)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(2205..2221)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 2229..2245
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(2253..2283)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2298..2319
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(2607..3195)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3366..4226)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4227..4331)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
661 ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcatgggg tcgtgcgctc
721 ctttcggtcg ggcgctgcgg gtcgtggggc gggcgtcagg caccgggctt gcgggtcatg
781 caccaggtgc gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa gccgagccgc
841 tcgtagaagg ggaggttgcg gggcgcggag gtctccagga aggcgggcac cccggcgcgc
901 tcggccgcct ccactccggg gagcacgacg gcgctgccca gacccttgcc ctggtggtcg
961 ggcgagacgc cgacggtggc caggaaccac gcgggctcct tgggccggtg cggcgccagg
1021 aggccttcca tctgttgctg cgcggccagc cgggaaccgc tcaactcggc catgcgcggg
1081 ccgatctcgg cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca gaccgccacc
1141 gcggcgccgt cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg cgtgaggaag
1201 agttcttgca gctcggtgac ccgctcgatg tggcggtccg gatcgacggt gtggcgcgtg
1261 gcggggtagt cggcgaacgc ggcggcgagg gtgcgtacgg ccctggggac gtcgtcgcgg
1321 gtggcgaggc gcaccgtggg cttgtactcg gtcatggtaa gctagcttgg gctgcaggtc
1381 gaaaggcccg gagatgagga agaggagaac agcgcggcag acgtgcgctt ttgaagcgtg
1441 cagaatgccg ggcctccgga ggaccttcgg gcgcccgccc cgcccctgag cccgcccctg
1501 agcccgcccc cggacccacc ccttcccagc ctctgagccc agaaagcgaa ggagcaaagc
1561 tgctattggc cgctgcccca aaggcctacc cgcttccatt gctcagcggt gctgtccatc
1621 tgcacgagac tagtgagacg tgctacttcc atttgtcacg tcctgcacga cgcgagctgc
1681 ggggcggggg ggaacttcct gactagggga ggagtagaag gtggcgcgaa ggggccacca
1741 aagaacggag ccggttggcg cctaccggtg gatgtggaat gtgtgcgagg ccagaggcca
1801 cttgtgtagc gccaagtgcc cagcggggct gctaaagcgc atgctccaga ctgccttggg
1861 aaaagcgcct cccctacccg gtagaattcg aacgctgacg tcatcaaccc gctccaagga
1921 atcgcgggcc cagtgtcact aggcgggaac acccagcgcg cgtgcgccct ggcaggaaga
1981 tggctgtgag ggacagggga gtggcgccct gcaatatttg catgtcgcta tgtgttctgg
2041 gaaatcacca taaacgtgaa atgtctttgg atttgggaat cttataagtt ccctatcagt
2101 gatagagatc taagcttatc gataccgtcg acctcgaggg ggggcccggt acccagcttt
2161 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct
2221 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
2281 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
2341 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
2401 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
2461 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
2521 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
2581 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
2641 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
2701 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
2761 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
2821 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
2881 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
2941 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
3001 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
3061 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
3121 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
3181 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
3241 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
3301 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
3361 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
3421 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
3481 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
3541 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
3601 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
3661 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
3721 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
3781 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
3841 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
3901 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
3961 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
4021 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
4081 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
4141 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
4201 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
4261 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
4321 ggggttccgc gcacatttcc ccgaaaagtg ccac
//
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