| 出品公司: | Promega |
|---|---|
| 载体名称: | pGL4.79 ; pGL4.79[hRluc/Neo] |
| 质粒类型: | 信号通路报告载体；哺乳动物载体；海肾荧光素酶报告载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | 无 |
| 载体大小: | 4879 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | hRluc |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | Neomycin |
| 克隆菌株: | TOP10等常规菌株 |
| 宿主细胞（系）: | HEK293等 |
| 备注: | pGL4.79[hRluc/Neo]载体是信号通路报告载体；无启动子；含海肾荧光素酶报告基因hRluc。 |
| 产品目录号: | E6971 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pGL4.79载体基本信息
pGL4.79载体图谱质粒图谱和多克隆位点信息
pGL4.79载体简介
The pGL4.79[hRluc/Neo] Vector encodes the luciferase reporter gene hRluc (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker for neomycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. The pGL4 Vectors are engineered with fewer consensus regulatory sequences than the pGL3 Vectors and a synthetic reporter gene that has been codon optimized for mammalian expression. The pGL4.79[hRluc/Neo] Vector is a basic vector with no promoter. However, it contains a multiple cloning region that allows cloning of a promoter of choice.
pGL4.79载体序列
LOCUS DQ188843 4879 bp DNA circular SYN 16-NOV-2005
DEFINITION Renilla luciferase reporter vector pGL4.79[hRluc/Neo], complete
sequence.
ACCESSION DQ188843
VERSION DQ188843.1 GI:76364302
KEYWORDS .
SOURCE Renilla luciferase reporter vector pGL4.79[hRluc/Neo]
ORGANISM Renilla luciferase reporter vector pGL4.79[hRluc/Neo]
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4879)
AUTHORS Stecha,P., Paguio,A. and Chauvin,F.
TITLE pGL4 Luciferase Reporter Vectors Technical Manual, TM259, Promega
Corporation
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 4879)
AUTHORS Stecha,P., Paguio,A. and Chauvin,F.
TITLE Direct Submission
JOURNAL Submitted (31-AUG-2005) Scientific Communications, Promega
Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
FEATURES Location/Qualifiers
source 1..4879
/organism="Renilla luciferase reporter vector
pGL4.79[hRluc/Neo]"
/mol_type="other DNA"
/db_xref="taxon:346090"
misc_feature 1..70
/note="multiple cloning region"
CDS 100..1035
/codon_start=1
/product="synthetic Renilla luciferase"
/protein_id="ABA41671.1"
/db_xref="GI:76364303"
/translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
regulatory 726..4879
/regulatory_class="polyA_signal_sequence"
/note="synthetic poly(A) signal/transcriptional pause
site"
regulatory 1336..1754
/regulatory_class="promoter"
/note="SV40 early enhancer/promoter"
CDS 1779..2573
/note="neomycin resistance"
/codon_start=1
/product="neomycin phosphotransferase"
/protein_id="ABA41672.1"
/db_xref="GI:76364304"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
regulatory 2598..2646
/regulatory_class="polyA_signal_sequence"
/note="synthetic poly(A) signal"
primer_bind 2713..2732
/note="Reporter Vector primer 4 (RVprimer4) binding
region"
rep_origin 2970
/note="ColEI-derived plasmid replication origin"
gene complement(3761..4621)
/gene="bla"
CDS complement(3761..4621)
/gene="bla"
/note="ampicillin resistance"
/codon_start=1
/product="beta-lactamase"
/protein_id="ABA41673.1"
/db_xref="GI:76364305"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
primer_bind 4828..4847
/note="Reporter Vector primer 3 (RVprimer3) binding
region"
ORIGIN
1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggcttccaa ggtgtacgac
121 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg
181 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg
241 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc
301 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc
361 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg
421 ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt
481 cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg
541 gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag
601 agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca
661 agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag
721 gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag
781 ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc
841 gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc
901 gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc
961 caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg
1021 aagaacgagc agtaattcta gagtcggggc ggccggccgc ttcgagcaga catgataaga
1081 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
1141 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
1201 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
1261 agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccgtttgc gtattgggcg
1321 ctcttccgct gatctgcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt
1381 tagctacctt ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
1441 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
1501 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
1561 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
1621 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
1681 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
1741 tttgcaaaaa gctcgattct tctgacacta gcgccaccat gatcgaacaa gacggcctcc
1801 atgctggcag tcccgcagct tgggtcgaac gcttgttcgg gtacgactgg gcccagcaga
1861 ccatcggatg tagcgatgcg gccgtgttcc gtctaagcgc tcaaggccgg cccgtgctgt
1921 tcgtgaagac cgacctgagc ggcgccctga acgagcttca agacgaggct gcccgcctga
1981 gctggctggc caccaccggc gtaccctgcg ccgctgtgtt ggatgttgtg accgaagccg
2041 gccgggactg gctgctgctg ggcgaggtcc ctggccagga tctgctgagc agccaccttg
2101 cccccgctga gaaggtttct atcatggccg atgcaatgcg gcgcctgcac accctggacc
2161 ccgctacctg ccccttcgac caccaggcta agcatcggat cgagcgtgct cggacccgca
2221 tggaggccgg cctggtggac caggacgacc tggacgagga gcatcagggc ctggcccccg
2281 ctgaactgtt cgcccgactg aaagcccgca tgccggacgg tgaggacctg gttgtcacac
2341 acggagatgc ctgcctccct aacatcatgg tcgagaatgg ccgcttctcc ggcttcatcg
2401 actgcggtcg cctaggagtt gccgaccgct accaggacat cgccctggcc acccgcgaca
2461 tcgctgagga gcttggcggc gagtgggccg accgcttctt agtcttgtac ggcatcgcag
2521 ctcccgacag ccagcgcatc gccttctacc gcttgctcga cgagttcttt taatgatcta
2581 gaaccggtca tggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt
2641 tgtgtgttcg aactagatgc tgtcgaccga tgcccttgag agccttcaac ccagtcagct
2701 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca
2761 tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt cctcgctcac tgactcgctg
2821 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
2881 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
2941 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
3001 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
3061 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
3121 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
3181 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
3241 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
3301 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
3361 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
3421 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
3481 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
3541 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
3601 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
3661 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
3721 tggtctgaca gcggccgcaa atgctaaacc actgcagtgg ttaccagtgc ttgatcagtg
3781 aggcaccgat ctcagcgatc tgcctatttc gttcgtccat agtggcctga ctccccgtcg
3841 tgtagatcac tacgattcgt gagggcttac catcaggccc cagcgcagca atgatgccgc
3901 gagagccgcg ttcaccggcc cccgatttgt cagcaatgaa ccagccagca gggagggccg
3961 agcgaagaag tggtcctgct actttgtccg cctccatcca gtctatgagc tgctgtcgtg
4021 atgctagagt aagaagttcg ccagtgagta gtttccgaag agttgtggcc attgctactg
4081 gcatcgtggt atcacgctcg tcgttcggta tggcttcgtt caactctggt tcccagcggt
4141 caagccgggt cacatgatca cccatattat gaagaaatgc agtcagctcc ttagggcctc
4201 cgatcgttgt cagaagtaag ttggccgcgg tgttgtcgct catggtaatg gcagcactac
4261 acaattctct taccgtcatg ccatccgtaa gatgcttttc cgtgaccggc gagtactcaa
4321 ccaagtcgtt ttgtgagtag tgtatacggc gaccaagctg ctcttgcccg gcgtctatac
4381 gggacaacac cgcgccacat agcagtactt tgaaagtgct catcatcggg aatcgttctt
4441 cggggcggaa agactcaagg atcttgccgc tattgagatc cagttcgata tagcccactc
4501 ttgcacccag ttgatcttca gcatctttta ctttcaccag cgtttcgggg tgtgcaaaaa
4561 caggcaagca aaatgccgca aagaagggaa tgagtgcgac acgaaaatgt tggatgctca
4621 tactcgtcct ttttcaatat tattgaagca tttatcaggg ttactagtac gtctctcaag
4681 gataagtaag taatattaag gtacgggagg tattggacag gccgcaataa aatatcttta
4741 ttttcattac atctgtgtgt tggttttttg tgtgaatcga tagtactaac atacgctctc
4801 catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca
4861 ggtgccagaa catttctct
//
