pGL4.79[hRluc/Neo]

价格:1500元

联系方式:I47-825O-882O

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pGL4.79载体基本信息

出品公司: Promega
载体名称: pGL4.79 ; pGL4.79[hRluc/Neo]
质粒类型: 信号通路报告载体;哺乳动物载体;海肾荧光素酶报告载体
高拷贝/低拷贝: --
克隆方法: 限制性内切酶,多克隆位点
启动子:
载体大小: 4879 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: hRluc
载体抗性: 氨苄青霉素
筛选标记: Neomycin
克隆菌株: TOP10等常规菌株
宿主细胞(系): HEK293等
备注: pGL4.79[hRluc/Neo]载体是信号通路报告载体;无启动子;含海肾荧光素酶报告基因hRluc。
产品目录号: E6971
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

pGL4.79载体质粒图谱和多克隆位点信息

pGL4.79载体图谱



pGL4.79 载体特征

pGL4.79载体简介

The pGL4.79[hRluc/Neo] Vector encodes the luciferase reporter gene hRluc (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker for neomycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. The pGL4 Vectors are engineered with fewer consensus regulatory sequences than the pGL3 Vectors and a synthetic reporter gene that has been codon optimized for mammalian expression. The pGL4.79[hRluc/Neo] Vector is a basic vector with no promoter. However, it contains a multiple cloning region that allows cloning of a promoter of choice.

pGL4.79载体序列

LOCUS       DQ188843                4879 bp    DNA     circular SYN 16-NOV-2005
DEFINITION  Renilla luciferase reporter vector pGL4.79[hRluc/Neo], complete
            sequence.
ACCESSION   DQ188843
VERSION     DQ188843.1  GI:76364302
KEYWORDS    .
SOURCE      Renilla luciferase reporter vector pGL4.79[hRluc/Neo]
  ORGANISM  Renilla luciferase reporter vector pGL4.79[hRluc/Neo]
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Stecha,P., Paguio,A. and Chauvin,F.
  TITLE     pGL4 Luciferase Reporter Vectors Technical Manual, TM259, Promega
            Corporation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4879)
  AUTHORS   Stecha,P., Paguio,A. and Chauvin,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2005) Scientific Communications, Promega
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Renilla luciferase reporter vector
                     pGL4.79[hRluc/Neo]"
                     /mol_type="other DNA"
                     /db_xref="taxon:346090"
     misc_feature    1..70
                     /note="multiple cloning region"
     CDS             100..1035
                     /codon_start=1
                     /product="synthetic Renilla luciferase"
                     /protein_id="ABA41671.1"
                     /db_xref="GI:76364303"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
                     NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
                     AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
                     EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
                     WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
                     EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     regulatory      726..4879
                     /regulatory_class="polyA_signal_sequence"
                     /note="synthetic poly(A) signal/transcriptional pause
                     site"
     regulatory      1336..1754
                     /regulatory_class="promoter"
                     /note="SV40 early enhancer/promoter"
     CDS             1779..2573
                     /note="neomycin resistance"
                     /codon_start=1
                     /product="neomycin phosphotransferase"
                     /protein_id="ABA41672.1"
                     /db_xref="GI:76364304"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      2598..2646
                     /regulatory_class="polyA_signal_sequence"
                     /note="synthetic poly(A) signal"
     primer_bind     2713..2732
                     /note="Reporter Vector primer 4 (RVprimer4) binding
                     region"
     rep_origin      2970
                     /note="ColEI-derived plasmid replication origin"
     gene            complement(3761..4621)
                     /gene="bla"
     CDS             complement(3761..4621)
                     /gene="bla"
                     /note="ampicillin resistance"
                     /codon_start=1
                     /product="beta-lactamase"
                     /protein_id="ABA41673.1"
                     /db_xref="GI:76364305"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     primer_bind     4828..4847
                     /note="Reporter Vector primer 3 (RVprimer3) binding
                     region"
ORIGIN      
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggcttccaa ggtgtacgac
      121 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg
      181 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg
      241 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc
      301 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc
      361 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg
      421 ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt
      481 cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg
      541 gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag
      601 agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca
      661 agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag
      721 gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag
      781 ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc
      841 gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc
      901 gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc
      961 caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg
     1021 aagaacgagc agtaattcta gagtcggggc ggccggccgc ttcgagcaga catgataaga
     1081 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     1141 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     1201 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     1261 agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccgtttgc gtattgggcg
     1321 ctcttccgct gatctgcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt
     1381 tagctacctt ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
     1441 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     1501 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     1561 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1621 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1681 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1741 tttgcaaaaa gctcgattct tctgacacta gcgccaccat gatcgaacaa gacggcctcc
     1801 atgctggcag tcccgcagct tgggtcgaac gcttgttcgg gtacgactgg gcccagcaga
     1861 ccatcggatg tagcgatgcg gccgtgttcc gtctaagcgc tcaaggccgg cccgtgctgt
     1921 tcgtgaagac cgacctgagc ggcgccctga acgagcttca agacgaggct gcccgcctga
     1981 gctggctggc caccaccggc gtaccctgcg ccgctgtgtt ggatgttgtg accgaagccg
     2041 gccgggactg gctgctgctg ggcgaggtcc ctggccagga tctgctgagc agccaccttg
     2101 cccccgctga gaaggtttct atcatggccg atgcaatgcg gcgcctgcac accctggacc
     2161 ccgctacctg ccccttcgac caccaggcta agcatcggat cgagcgtgct cggacccgca
     2221 tggaggccgg cctggtggac caggacgacc tggacgagga gcatcagggc ctggcccccg
     2281 ctgaactgtt cgcccgactg aaagcccgca tgccggacgg tgaggacctg gttgtcacac
     2341 acggagatgc ctgcctccct aacatcatgg tcgagaatgg ccgcttctcc ggcttcatcg
     2401 actgcggtcg cctaggagtt gccgaccgct accaggacat cgccctggcc acccgcgaca
     2461 tcgctgagga gcttggcggc gagtgggccg accgcttctt agtcttgtac ggcatcgcag
     2521 ctcccgacag ccagcgcatc gccttctacc gcttgctcga cgagttcttt taatgatcta
     2581 gaaccggtca tggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt
     2641 tgtgtgttcg aactagatgc tgtcgaccga tgcccttgag agccttcaac ccagtcagct
     2701 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca
     2761 tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt cctcgctcac tgactcgctg
     2821 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     2881 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     2941 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     3001 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     3061 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     3121 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     3181 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     3241 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     3301 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     3361 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     3421 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     3481 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     3541 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     3601 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     3661 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     3721 tggtctgaca gcggccgcaa atgctaaacc actgcagtgg ttaccagtgc ttgatcagtg
     3781 aggcaccgat ctcagcgatc tgcctatttc gttcgtccat agtggcctga ctccccgtcg
     3841 tgtagatcac tacgattcgt gagggcttac catcaggccc cagcgcagca atgatgccgc
     3901 gagagccgcg ttcaccggcc cccgatttgt cagcaatgaa ccagccagca gggagggccg
     3961 agcgaagaag tggtcctgct actttgtccg cctccatcca gtctatgagc tgctgtcgtg
     4021 atgctagagt aagaagttcg ccagtgagta gtttccgaag agttgtggcc attgctactg
     4081 gcatcgtggt atcacgctcg tcgttcggta tggcttcgtt caactctggt tcccagcggt
     4141 caagccgggt cacatgatca cccatattat gaagaaatgc agtcagctcc ttagggcctc
     4201 cgatcgttgt cagaagtaag ttggccgcgg tgttgtcgct catggtaatg gcagcactac
     4261 acaattctct taccgtcatg ccatccgtaa gatgcttttc cgtgaccggc gagtactcaa
     4321 ccaagtcgtt ttgtgagtag tgtatacggc gaccaagctg ctcttgcccg gcgtctatac
     4381 gggacaacac cgcgccacat agcagtactt tgaaagtgct catcatcggg aatcgttctt
     4441 cggggcggaa agactcaagg atcttgccgc tattgagatc cagttcgata tagcccactc
     4501 ttgcacccag ttgatcttca gcatctttta ctttcaccag cgtttcgggg tgtgcaaaaa
     4561 caggcaagca aaatgccgca aagaagggaa tgagtgcgac acgaaaatgt tggatgctca
     4621 tactcgtcct ttttcaatat tattgaagca tttatcaggg ttactagtac gtctctcaag
     4681 gataagtaag taatattaag gtacgggagg tattggacag gccgcaataa aatatcttta
     4741 ttttcattac atctgtgtgt tggttttttg tgtgaatcga tagtactaac atacgctctc
     4801 catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca
     4861 ggtgccagaa catttctct
//

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