| 质粒名称: | VSV.G |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 14888 |
| 存储实验室: | Tannishtha Reya |
|
载体骨架基本信息 |
|
| 载体名称: | pCI |
| 载体出品公司: | Promega |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4006 bp |
| 完整质粒大小: | 5600 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | VSV-G Envelope,vesicular stomatitis virus G glycoprotein |
| 物种来源: | -- |
| 插入大小: | 1568 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI(未破坏) |
| 3' 克隆位点: | XbaI(未破坏) |
| 5' 测序引物 | chim-int-F (5'-TCTTACTGACATCCACTTTGCC-3') |
| 3' 测序引物 | EBV-rev |
|
文献引用方法 |
|
| 材料和方法部分: | VSV.G was a gift from Tannishtha Reya (Addgene plasmid # 14888 ; http://n2t.net/addgene:14888 ; RRID:Addgene_14888) |
| 参考文献部分: | A role for Wnt signalling in self-renewal of haematopoietic stem cells. Reya T, Duncan AW, Ailles L, Domen J, Scherer DC, Willert K, Hintz L, Nusse R, Weissman IL. Nature. 2003 May 22. 423(6938):409-14. 10.1038/nature01593 PubMed 12717450 |
买家导航
VSVG载体基本信息
VSVG载体图谱质粒图谱和多克隆位点信息
VSVG载体简介
VSVG载体序列
LOCUS VSV.G 5551 bp ds-DNA circular SYN 13-SEP-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS VSV.G
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5551)
AUTHORS Reya T, Duncan AW, Ailles L, Domen J, Scherer DC, Willert K, Hintz
L, Nusse R, Weissman IL
TITLE A role for Wnt signalling in self-renewal of haematopoietic stem
cells.
JOURNAL Nature. 2003 May 22. 423(6938):409-14.
PUBMED 12717450
REFERENCE 2 (bases 1 to 5551)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 13, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5551
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 114..493
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 494..697
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 648..668
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 694..718
/label=LNCX
/note="Human CMV promoter, forward primer"
intron 833..965
/label=chimeric intron
/note="chimera between introns from human beta-globin and
immunoglobulin heavy chain genes"
primer_bind 948..967
/label=pMT2-F
/note="Synthetic intron, forward primer"
promoter 1010..1028
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 1052..2587
/codon_start=1
/product="vesicular stomatitis virus G glycoprotein"
/label=VSV-G
/note="Indiana strain"
/translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
polyA_signal 2641..2762
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2652..2671)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 2706..2725
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
rep_origin 2943..3398
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3030..3049)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3240..3261
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(3465..3484)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 3584..3606
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(3644..3662)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 3730..3834
/gene="bla"
/label=AmpR promoter
CDS 3835..4695
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4053..4072)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 4866..5454
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5355..5374
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 attcattggt tatatagcat aaatcaatat tggctattgg ccattgcata cgttgtatct
61 atatcataat atgtacattt atattggctc atgtccaata tgaccgccat gttggcattg
121 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
181 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
241 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
301 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
361 tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
421 tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat
481 cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat agcggtttga
541 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
601 aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg
661 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcac
721 tagaagcttt attgcggtag tttatcacag ttaaattgct aacgcagtca gtgcttctga
781 cacaacagtc tcgaacttaa gctgcagaag ttggtcgtga ggcactgggc aggtaagtat
841 caaggttaca agacaggttt aaggagacca atagaaactg ggcttgtcga gacagagaag
901 actcttgcgt ttctgatagg cacctattgg tcttactgac atccactttg cctttctctc
961 cacaggtgtc cactcccagt tcaattacag ctcttaaggc tagagtactt aatacgactc
1021 actataggct agcctcgaga attctgacac tatgaagtgc cttttgtact tagccttttt
1081 attcattggg gtgaattgca agttcaccat agtttttcca cacaaccaaa aaggaaactg
1141 gaaaaatgtt ccttctaatt accattattg cccgtcaagc tcagatttaa attggcataa
1201 tgacttaata ggcacagcct tacaagtcaa aatgcccaag agtcacaagg ctattcaagc
1261 agacggttgg atgtgtcatg cttccaaatg ggtcactact tgtgatttcc gctggtatgg
1321 accgaagtat ataacacatt ccatccgatc cttcactcca tctgtagaac aatgcaagga
1381 aagcattgaa caaacgaaac aaggaacttg gctgaatcca ggcttccctc ctcaaagttg
1441 tggatatgca actgtgacgg atgccgaagc agtgattgtc caggtgactc ctcaccatgt
1501 gctggttgat gaatacacag gagaatgggt tgattcacag ttcatcaacg gaaaatgcag
1561 caattacata tgccccactg tccataactc tacaacctgg cattctgact ataaggtcaa
1621 agggctatgt gattctaacc tcatttccat ggacatcacc ttcttctcag aggacggaga
1681 gctatcatcc ctgggaaagg agggcacagg gttcagaagt aactactttg cttatgaaac
1741 tggaggcaag gcctgcaaaa tgcaatactg caagcattgg ggagtcagac tcccatcagg
1801 tgtctggttc gagatggctg ataaggatct ctttgctgca gccagattcc ctgaatgccc
1861 agaagggtca agtatctctg ctccatctca gacctcagtg gatgtaagtc taattcagga
1921 cgttgagagg atcttggatt attccctctg ccaagaaacc tggagcaaaa tcagagcggg
1981 tcttccaatc tctccagtgg atctcagcta tcttgctcct aaaaacccag gaaccggtcc
2041 tgctttcacc ataatcaatg gtaccctaaa atactttgag accagataca tcagagtcga
2101 tattgctgct ccaatcctct caagaatggt cggaatgatc agtggaacta ccacagaaag
2161 ggaactgtgg gatgactggg caccatatga agacgtggaa attggaccca atggagttct
2221 gaggaccagt tcaggatata agtttccttt atacatgatt ggacatggta tgttggactc
2281 cgatcttcat cttagctcaa aggctcaggt gttcgaacat cctcacattc aagacgctgc
2341 ttcgcaactt cctgatgatg agagtttatt ttttggtgat actgggctat ccaaaaatcc
2401 aatcgagctt gtagaaggtt ggttcagtag ttggaaaagc tctattgcct cttttttctt
2461 tatcataggg ttaatcattg gactattctt ggttctccga gttggtatcc atctttgcat
2521 taaattaaag cacaccaaga aaagacagat ttatacagac atagagatga accgacttgg
2581 aaagtaactc aaatcctgca ctctagagtc gacccgggcg gccgcttcga gcagacatga
2641 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
2701 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
2761 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt
2821 tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatccg ggctggcgta
2881 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
2941 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
3001 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
3061 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
3121 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
3181 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
3241 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
3301 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
3361 taacgcgaat tttaacaaaa tattaacgct tacaatttcc tgatgcggta ttttctcctt
3421 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
3481 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
3541 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
3601 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
3661 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
3721 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
3781 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
3841 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
3901 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
3961 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
4021 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
4081 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
4141 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
4201 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
4261 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
4321 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
4381 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
4441 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
4501 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
4561 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
4621 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
4681 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
4741 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
4801 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
4861 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
4921 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
4981 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
5041 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
5101 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
5161 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
5221 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
5281 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
5341 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
5401 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
5461 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catggctcga
5521 cagatcttca atattggcca ttagccatat t
//
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