pET102/D-TOPO

价格:2500元

联系方式:I47-825O-882O

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pET102载体基本信息

出品公司: Invitrogen
载体名称: pET102 ; pET102/D-TOPO
质粒类型: 大肠杆菌表达载体;细菌表达载体;TOPO载体
高拷贝/低拷贝: 低拷贝
克隆方法: TOPO
启动子: T7/lacO
载体大小: 6315 bp
5' 测序引物及序列: TrxFus fwd 5’ TTCCTCGACGCTAACCTG 3’
3' 测序引物及序列: T7 rev 5’ TAGTTATTGCTCAGCGGTGG 3’
载体标签: 硫氧还蛋白Trx tag (N-term);V5 tag、6xHis tag (C-term)
载体抗性: 氨苄青霉素
克隆菌株: TOP10
表达菌株:
BL21-Star(DE3) 
BL21-Star(DE3) pLysS
BL21-TrxB(DE3)
Origami2(DE3)
 
备注:
pET102含有T7聚合酶结合位点和RBS核糖体结合位点,可以做体外转录和翻译;

硫氧还蛋白Trx 标签可以帮助难溶解的目的蛋白折叠,提高可溶性蛋白产量。
 
产品目录号: K10201
组成型/诱导型: 诱导型(IPTG)
病毒/非病毒: 非病毒

pET102载体质粒图谱和多克隆位点信息

pET102-D-TOPO载体图谱



pET102-D-TOPO多克隆位点

pET102载体简介

Champion™ pET102 Directional TOPO® Expression Kit

The Champion™ pET Expression System yields the highest-level protein production in 
E. coli. During expression, your protein of interest can reach levels greater than 
50 percent of total cellular protein. Based on T7 expression vectors originally 
developed by Studier and colleagues (1-3), high-level expression is achieved 
because the T7 RNA polymerase is more processive than native E. coliRNA polymerase 
and is dedicated to the transcription of your gene of interest. Protein production 
is further enhanced in the system by the expression strain BL21 Star™ E. coli, 
which significantly improves the stability of mRNA transcripts and increases protein 
expression up to ten-fold.
Simplified, efficient Directional TOPO® cloning allows you to quickly enter a 
Champion™ pET Expression vector. These kits feature linearized, topoisomerase 
I-activated Champion™ pET expression vectors for five-minute directional cloning. 
Directional TOPO® Cloning technology facilitates gene expression because:

• A proofreading enzyme is used for PCR, resulting in fewer errors in cloned genes
• Greater than 90% of the clones are in the correct orientation for gene expression, 
reducing the time spent on colony screening

Seven Champion™ pET Directional TOPO® Expression Vectors are available: Each vector 
carries a T7lac promoter for high-level expression. Flexible options for simplifying 
protein detection, cleaving purification tags, selecting plasmid carrying clones, 
and/or improving protein yields are available.
With the Champion™ pET Directional TOPO® vectors you can expect highest-level protein 
production. Figure 2 shows expression of the lacZ gene in Champion™ pET Directional 
TOPO® vectors.  demonstrates efficient cleavage using TEV protease of the 
N-terminal tag of a β-galactosidase fusion protein expressed from pET151/D-TOPO®.

pET102载体序列

LOCUS       pET102 Directional TOPO	6315 bp 	DNA	SYN
DEFINITION  pET102 Directional TOPO
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6315
                     /organism="pET102 Directional TOPO"
                     /mol_type="other DNA"
     misc_feature    106..125
                     /label="pBRrevBam_primer"
     promoter        209..227
                     /label="T7_promoter"
     misc_feature    227..254
                     /label="lacO"
     CDS             complement(256..768)
                     /label="ORF frame 1"
                     /translation="MVMVMMTGTRRIETEERVRDRLTFKLELALGEGINSQLVIVITG
                     SRAGQVSVEELFQLTFRQCTHFGCRHFTVFEQQQSRDTTDAIFRRSARVVIDVQFCNG
                     QFALIFVSDFIQNRSDHFARTAPVCPEINQDCTICLKYISIKRIISQMNNFIRSHYVY
                     LLLKVKQNYF*"
     misc_feature    272..288
                     /label="T7_transl_en_RBS"
                     /translation="MVMVMMTGTRRIETEERVRDRLTFKLELALGEGINSQLVIVITG
                     SRAGQVSVEELFQLTFRQCTHFGCRHFTVFEQQQSRDTTDAIFRRSARVVIDVQFCNG
                     QFALIFVSDFIQNRSDHFARTAPVCPEINQDCTICLKYISIKRIISQMNNFIRSHYVY
                     LLLKVKQNYF*"
     misc_feature    298..626
                     /label="His_patch_thio"
                     /translation="MVMVMMTGTRRIETEERVRDRLTFKLELALGEGINSQLVIVITG
                     SRAGQVSVEELFQLTFRQCTHFGCRHFTVFEQQQSRDTTDAIFRRSARVVIDVQFCNG
                     QFALIFVSDFIQNRSDHFARTAPVCPEINQDCTICLKYISIKRIISQMNNFIRSHYVY
                     LLLKVKQNYF*"
     CDS             298..771
                     /label="ORF frame 1"
                     /translation="MGSDKIIHLTDDSFDTDVLKADGAILVDFWAHWCGPCKMIAPIL
                     DEIADEYQGKLTVAKLNIDHNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLK
                     EFLDANLAGSGSGDDDDKLGIDPFTKGELKLEGKPIPNPLLGLDSTRTGHHHHHH*"
     misc_feature    643..657
                     /label="EK"
                     /translation="MGSDKIIHLTDDSFDTDVLKADGAILVDFWAHWCGPCKMIAPIL
                     DEIADEYQGKLTVAKLNIDHNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLK
                     EFLDANLAGSGSGDDDDKLGIDPFTKGELKLEGKPIPNPLLGLDSTRTGHHHHHH*"
     misc_feature    700..741
                     /label="V5"
                     /translation="MGSDKIIHLTDDSFDTDVLKADGAILVDFWAHWCGPCKMIAPIL
                     DEIADEYQGKLTVAKLNIDHNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLK
                     EFLDANLAGSGSGDDDDKLGIDPFTKGELKLEGKPIPNPLLGLDSTRTGHHHHHH*"
     misc_feature    751..768
                     /label="6xHIS"
                     /translation="MGSDKIIHLTDDSFDTDVLKADGAILVDFWAHWCGPCKMIAPIL
                     DEIADEYQGKLTVAKLNIDHNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLK
                     EFLDANLAGSGSGDDDDKLGIDPFTKGELKLEGKPIPNPLLGLDSTRTGHHHHHH*"
     terminator      783..911
                     /label="T7_terminator"
                     /translation="MGSDKIIHLTDDSFDTDVLKADGAILVDFWAHWCGPCKMIAPIL
                     DEIADEYQGKLTVAKLNIDHNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLK
                     EFLDANLAGSGSGDDDDKLGIDPFTKGELKLEGKPIPNPLLGLDSTRTGHHHHHH*"
     misc_feature    complement(825..843)
                     /label="T7_Terminal_primer"
                     /translation="MGSDKIIHLTDDSFDTDVLKADGAILVDFWAHWCGPCKMIAPIL
                     DEIADEYQGKLTVAKLNIDHNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLK
                     EFLDANLAGSGSGDDDDKLGIDPFTKGELKLEGKPIPNPLLGLDSTRTGHHHHHH*"
     promoter        1436..1464
                     /label="AmpR_promoter"
                     /translation="MGSDKIIHLTDDSFDTDVLKADGAILVDFWAHWCGPCKMIAPIL
                     DEIADEYQGKLTVAKLNIDHNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLK
                     EFLDANLAGSGSGDDDDKLGIDPFTKGELKLEGKPIPNPLLGLDSTRTGHHHHHH*"
     gene            1506..2366
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MGSDKIIHLTDDSFDTDVLKADGAILVDFWAHWCGPCKMIAPIL
                     DEIADEYQGKLTVAKLNIDHNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLK
                     EFLDANLAGSGSGDDDDKLGIDPFTKGELKLEGKPIPNPLLGLDSTRTGHHHHHH*"
     CDS             1506..2366
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      2521..3140
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    3536..3558
                     /label="pGEX_3_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    complement(3552..3743)
                     /label="ROP"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     CDS             complement(4382..4999)
                     /label="ORF frame 3"
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSIIG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     CDS             4577..5272
                     /label="ORF frame 2"
                     /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ
                     RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER
                     CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSRSRCLMSELTYINCVAL
                     TARFPVGKPVVPAALMNRPTRGERRFAYWAPGWFFFSPVRRATADCPSPPGPERVAAS
                     GPRWFAPAGENPV*"
     misc_feature    complement(5055..6146)
                     /label="lacI"
                     /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ
                     RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER
                     CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSRSRCLMSELTYINCVAL
                     TARFPVGKPVVPAALMNRPTRGERRFAYWAPGWFFFSPVRRATADCPSPPGPERVAAS
                     GPRWFAPAGENPV*"
     CDS             complement(5055..6014)
                     /label="ORF frame 2"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     CDS             complement(5887..72)
                     /label="ORF frame 1"
                     /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
                     LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
                     RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
                     ANCRGD*"
ORIGIN
    1 CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA
   61 ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT
  121 ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA
  181 GAGGATCGAG ATCTCGATCC CGCGAAATTA ATACGACTCA CTATAGGGGA ATTGTGAGCG
  241 GATAACAATT CCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACATAATG
  301 GGATCTGATA AAATTATTCA TCTGACTGAT GATTCTTTTG ATACTGATGT ACTTAAGGCA
  361 GATGGTGCAA TCCTGGTTGA TTTCTGGGCA CACTGGTGCG GTCCGTGCAA AATGATCGCT
  421 CCGATTCTGG ATGAAATCGC TGACGAATAT CAGGGCAAAC TGACCGTTGC AAAACTGAAC
  481 ATCGATCACA ACCCGGGCAC TGCGCCGAAA TATGGCATCC GTGGTATCCC GACTCTGCTG
  541 CTGTTCAAAA ACGGTGAAGT GGCGGCAACC AAAGTGGGTG CACTGTCTAA AGGTCAGTTG
  601 AAAGAGTTCC TCGACGCTAA CCTGGCCGGC TCTGGATCCG GTGATGACGA TGACAAGCTG
  661 GGAATTGATC CCTTCACCAA GGGCGAGCTC AAGCTTGAAG GTAAGCCTAT CCCTAACCCT
  721 CTCCTCGGTC TCGATTCTAC GCGTACCGGT CATCATCACC ATCACCATTG AGTTTGATCC
  781 GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT
  841 AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC
  901 TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC
  961 CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA
 1021 CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG
 1081 CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC
 1141 CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT
 1201 TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT
 1261 AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTAATTC TTGAAGACGA AAGGGCCTCG
 1321 TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG
 1381 GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA
 1441 ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA
 1501 AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC
 1561 TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG
 1621 GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC
 1681 GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT
 1741 TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG
 1801 ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG
 1861 AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA
 1921 CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC
 1981 GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA
 2041 CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC
 2101 TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC
 2161 TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG
 2221 GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA
 2281 TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG
 2341 GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA
 2401 TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC
 2461 TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA
 2521 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA
 2581 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC
 2641 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT
 2701 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC
 2761 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC
 2821 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA
 2881 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG
 2941 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG
 3001 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT
 3061 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT
 3121 GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC
 3181 ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT
 3241 GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG
 3301 CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA
 3361 ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG
 3421 CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG
 3481 CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG
 3541 AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA
 3601 AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC
 3661 TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG
 3721 GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG
 3781 GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT
 3841 GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG
 3901 AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG
 3961 GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC
 4021 CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC
 4081 GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC
 4141 TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG
 4201 CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG
 4261 GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG
 4321 AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA
 4381 TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT
 4441 ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA
 4501 TCGCCGTGAC GATCAGCGGT CCAATGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC
 4561 CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG
 4621 GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG
 4681 TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT
 4741 GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA
 4801 AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT
 4861 CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA
 4921 CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT
 4981 TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT
 5041 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT
 5101 AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT
 5161 CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT
 5221 TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT
 5281 AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC
 5341 GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG
 5401 TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA
 5461 AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA
 5521 GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC
 5581 GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA
 5641 CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT
 5701 AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA
 5761 TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG
 5821 GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG
 5881 CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA
 5941 ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA
 6001 TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC
 6061 TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT
 6121 AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC
 6181 ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG
 6241 CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC
 6301 AAGGAATGGT GCATG
//

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