| 质粒名称: | pActPL-Gal4DBD |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 15304 |
| 存储实验室: | Benjamin White |
|
载体骨架基本信息 |
|
| 载体名称: | pActPL |
| 载体出品公司: | -- |
| 空载体大小: | 6400 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 昆虫表达 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄青霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | ZIP- Gal4DBD,RR12EE345L-Gal4DBD,GAL4 |
| 物种来源: | -- |
| 插入大小: | 640 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | NotI（未销毁） |
| 3’克隆位点: | AscI（未销毁） |
| 5’测序引物 | Gal4-Nterm |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pActPL-Gal4DBD was a gift from Benjamin White (Addgene plasmid # 15304 ; http://n2t.net/addgene:15304 ; RRID:Addgene_15304) |
| 参考文献部分: | Refined spatial manipulation of neuronal function by combinatorial restriction of transgene expression. Luan H, Peabody NC, Vinson CR, White BH. Neuron. 2006 Nov 9. 52(3):425-36. 10.1016/j.neuron.2006.08.028 PubMed 17088209 |
买家导航
pActPLGal4DBD载体基本信息
pActPLGal4DBD载体图谱质粒图谱和多克隆位点信息
pActPLGal4DBD载体简介
pActPLGal4DBD载体序列
LOCUS pActPL-Gal4DBD 7029 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pActPL-Gal4DBD
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7029)
AUTHORS Luan H, Peabody NC, Vinson CR, White BH
TITLE Refined spatial manipulation of neuronal function by combinatorial
restriction of transgene expression.
JOURNAL Neuron. 2006 Nov 9. 52(3):425-36.
PUBMED 17088209
REFERENCE 2 (bases 1 to 7029)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7029
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(4..21)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(4..20)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(13..35)
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind complement(229..248)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 348..370
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(408..426)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 494..598
/gene="bla"
/label=AmpR promoter
CDS 599..1459
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(817..836)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1630..2218
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2119..2138
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 2372..2389
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 2506..2527
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2542..2572
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2580..2596
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2585..2607
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 2604..2620
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2604..2620
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
CDS 5425..5865
/codon_start=1
/gene="Saccharomyces cerevisiae GAL4 (truncated)"
/product="DNA binding domain of the GAL4 transcriptional
activator"
/label=GAL4 DNA binding domain
/translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
1 ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
61 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
121 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct
181 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
241 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
301 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
361 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
421 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
1021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
1081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
1141 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
1201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
1261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
1321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
1381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
1441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
1501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
1561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
1621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
1681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
1741 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
1801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
1861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
1921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
1981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
2041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
2101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
2161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
2221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
2281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
2341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
2401 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
2461 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
2521 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
2581 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcttttt
2641 tttttttttt tttttttttt ttttaaaaaa aaatcatgaa tggcatcaac tctgaatcaa
2701 atctttgcag atgcacctac ttctcatttc cactgtcaca tcatttttcc agatctcgct
2761 gcctgttatg tggcccacaa accaagacac gttttatggc cattaaagct ggctgatcgt
2821 cgccaaacac caaatacata atgaatatgt acacattcga gaaagaagcg atcaaagaag
2881 cgtcttcggg cggagtagga gaatgcggag gagaaggaga acgagctgat ctagtatctc
2941 tccacaatcc aatgccaact gaccaactgg ccatattcgg agcaatttga agccaatttc
3001 catcgcctgg cgatcgctcc attcttggct atatgttttt caccgttccc ggggccattt
3061 tcaaagactc gtcggtaaga taagattgtg tcactcgctg tctctcttca tttgtcgaag
3121 aatgctgagg aatttcgcga tgacgtcggc gagtattttg aagaatgaga ataatttgta
3181 tttatacgaa aatcagttag tggaattttc tacaaaaaca tgttatctat agataatttt
3241 gttgcaaaat atgttgacta tgacaaagat tgtatgtata tacctttaat gtattctcat
3301 tttcttatgt atttataatg gcaatgatga tactgatgat attttaagat gatgccagac
3361 cacaggctga tttctgcgtc ttttgccgaa cgcagtgcat gtgcggttgt tgttttttgg
3421 aatagtttca attttcggac tgtccgcttt gatttcagtt tcttggctta ttcaaaaagc
3481 aaagtaaagc caaaaaagcg agatggcaat accaaatgcg gcaaaacggt agtggaagga
3541 aaggggtgcg gggcagcgga aggaagggtg gggcggggcg tggcggggtc tgtggctggg
3601 cgcgacgtca ccgacgttgg agccactcct ttgaccatgt gtgcgtgtgt gtattattcg
3661 tgtctcgcca ctcgccggtt gtttttttct ttttatctcg ctctctctag cgccatctcg
3721 tacgcatgct caacgcaccg catgttgccg tgtcctttat gcgtcatttt ggctcgaaat
3781 aggcaattat ttaaacaaag attagtcaac gaaaacgcta aaataaataa gtctacaata
3841 tggttactta ttgccatgtg tgtgcagcca acgatagcaa caaaagcaac aacacagtgg
3901 ctttccctct ttcacttttt gtttgcaagc gcgtgcgagc aagacggcac gaccggcaaa
3961 cgcaattacg ctgacaaaga gcagacgaag ttttggccga aaaacatcaa ggcgcctgat
4021 acgaatgcat ttgcaataac aattgcgata tttaatattg tttatgaagc tgtttgactt
4081 caaaacacac aaaaaaaaaa ataaaacaaa ttatttgaaa gagaattagg aatcggacag
4141 cttatcgtta cgggctaaca gcacaccgag acgaaatagc ttacctgacg tcacagcctc
4201 tggaagaact gccgccaagc agacgatgca gaggacgaca catagagtag cggagtaggc
4261 cagcgtagta cgcatgtgct tgtgtgtgag gcgtctctct cttcgtctcc tgtttgcgca
4321 aacgcataga ctgcactgag aaaatcgatt acctattttt tatgaatgaa tatttgcact
4381 attactattc aaaactatta agatagcaat cacattcaat agccaaatac tataccacct
4441 gagcgatgca acgaaatgat caatttgagc aaaaatgctg catatttagg acggcatcat
4501 tatagaaatg cttcttgctg tgtacttttc tctcgtctgg cagctgtttc gccgttattg
4561 ttaaaaccgg cttaagttag gtgtgttttc tacgactagc tagtgatgcc cctactagaa
4621 gatgtgtgtt gcacaaatgt ccctgaataa ccaatttgaa gtgcagatag cagtaaacgt
4681 aagctaatat gaatattatt taactgtaat gttttaatat cgctggacat tactaataaa
4741 cccactataa acacatgtac atatgtatgt tttggcatac aatgagtagt tggggaaaaa
4801 atgtgtaaaa gcaccgtgac catcacagca taaagataac cagctgaagt atcgaatatg
4861 agtaaccccc aaattgaatc acatgccgca actgatagga cccatggaag tacactcttc
4921 atggcgatat acaagacaca cacaagcacg aacacccagt tgcggaggaa attctccgta
4981 aatgaaaacc caatcggcga acaattcata cccatatatg gtaaaagttt tgaacgcgac
5041 ttgagagcgg agagcattgc ggctgataag gttttagcgc taagcgggct ttataaaacg
5101 ggctgcggga ccagttttca tatcactacc gtttgagttc ttgtgctgtg tggatactcc
5161 tcccgacaca aagccgctcc atcagccagc agtcgtctaa tccagagacc ccggatccga
5221 tagctgcggc cgcaaaacat gctggagatc cgcgccgcct tcctgcgcca gcgcaacacc
5281 gccctgcgca ccgaggtggc cgagctggag caggaggtgc agcgcctgga gaacgaggtg
5341 agccagtacg agacccgcta cggccccctg ggcggcggca aggctagcgg aggaggtggt
5401 ggaggtggag gtggaggtac tagtatgaag ctactgtctt ctatcgaaca agcatgcgat
5461 atttgccgac ttaaaaagct caagtgctcc aaagaaaaac cgaagtgcgc caagtgtctg
5521 aagaacaact gggagtgtcg ctactctccc aaaaccaaaa ggtctccgct gactagggca
5581 catctgacag aagtggaatc aaggctagaa agactggaac agctatttct actgattttt
5641 cctcgagaag accttgacat gattttgaaa atggattctt tacaggatat aaaagcattg
5701 ttaacaggat tatttgtaca agataatgtg aataaagatg ccgtcacaga tagattggct
5761 tcagtggaga ctgatatgcc tctaacattg agacagcata gaataagtgc gacatcatca
5821 tcggaagaga gtagtaacaa aggtcaaaga cagttgactg tatcgtaagg cgcgccataa
5881 ccgatccgtc gaccatgaag atcaagatca ttgccccgcc agagcgcaag tactctgtct
5941 ggatcggtgg ctccatcctg gcttcgctgt ccaccttcca gcagatgtgg atctccaagc
6001 aggagtacga cgagtccggc ccctccattg tgcaccgcaa gtgcttctaa gaaggatcgc
6061 ttgtctgggc aagaggatca ggatcgggat ggtcttgatt ctgctggagg aggaggagga
6121 gaagtcgagg aagcagcagc gaaagtgcaa gtgcgagtgg tggaagtttg gagtgcagca
6181 caacaaaatc aacaacaaca ccaactacaa gatgaaaaga gcggaaccac ctgcacacca
6241 tcatcactat catcatcgtt ttgggcgcat gttgtgtggt tccagcgtat taatataatt
6301 aatttattcc acatgagata tgatatgata tactatgtat tttttgtttt ttttttattt
6361 gtaaaccttt aatataacaa gaactacaaa aaagtgaaaa tgagcgaaaa tgcatattct
6421 gccattccac acacacacca acaacaccca acacacgcac acccacaagc ttacacacac
6481 acacattcgc ggcatgacaa ggacatcaag ataaagaaga acttaaagaa gatatttccc
6541 aaagcgcaaa aagaacacac acacattgca aaacacaaac aacacactag cgttttgtac
6601 aattcgtcag caaccttatg tattattttt taattatgat gtaattataa acaaagtgaa
6661 acaaaaatat gaaaacaaaa aggaaaatca aatctgtctt ctctttctcc cgctctcctc
6721 gctctctgct gctaacctcg ccctctcctc tctcatcttt ttgtctgtct ctcttcacat
6781 ttttgccggc cggcaaaata ataacccaca cacactcaca cttggctgca gtttcgcgtg
6841 cgatattcac acacattcaa gcatacatac atatgtattt tttttttatt tgtacacttt
6901 tctaattgca tgcgtatcga ttgataagtt tacgctgaaa atgttaatta aaatgtgaaa
6961 atgcaactga aaaactgatg aaatgaaaca acaacaagcg aacaagtcga cctgcaggca
7021 tgcaagctt
//
