| 质粒名称: | pLenti-PGK-KRAS4B(G12V) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 35633 |
| 存储实验室: | Daniel Haber |
|
载体骨架基本信息 |
|
| 载体名称: | pLenti-PGK-Hygro |
| 载体出品公司: | Eric Campeau |
| 载体抗性: | 氨苄西林 |
| 空载体大小: | 10000bp |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | 潮霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | K-Ras4B(G12V) |
| 备用名称: | KRAS4B |
| 物种来源: | 智人（人） |
| 插入大小: | 550 bp |
| 基因突变: | 甘氨酸12到缬氨酸 |
| Entrez基因: | KRAS (a.k.a. C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras2) |
| 启动子 | PGK |
| 标签/融合蛋白: | HA (N terminal on insert) |
| 克隆方法: | 网关克隆 |
| 5' 测序引物 | attL1 |
| 3' 测序引物 | attL2 |
|
文献引用方法 |
|
| 材料和方法部分: | pLenti-PGK-KRAS4B(G12V) was a gift from Daniel Haber (Addgene plasmid # 35633 ; http://n2t.net/addgene:35633 ; RRID:Addgene_35633) |
| 参考文献部分: |
TAK1 Inhibition Promotes Apoptosis in KRAS-Dependent Colon Cancers. Singh A, Sweeney MF, Yu M, Burger A, Greninger P, Benes C, Haber DA, Settleman J. Cell. 2012 Feb 17;148(4):639-50. 10.1016/j.cell.2011.12.033 PubMed 22341439 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS pLenti-PGK-KRAS4B(G12V) 9254 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pLenti-PGK-KRAS4B(G12V)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9254)
AUTHORS Singh A, Sweeney MF, Yu M, Burger A, Greninger P, Benes C, Haber DA,
Settleman J
TITLE TAK1 Inhibition Promotes Apoptosis in KRAS-Dependent Colon Cancers.
JOURNAL Cell. 2012 Feb 17;148(4):639-50.
PUBMED 22341439
REFERENCE 2 (bases 1 to 9254)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9254
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 107..128
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 143..173
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 181..197
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 186..208
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 205..221
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 205..221
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 240..260
/label=T3
/note="T3 promoter, forward primer"
promoter 242..260
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 288..514
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 515..695
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 742..867
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1360..1593
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2089..2206
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2252..2762
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 2681..2699
/label=hPGK-F
/note="Human PGK promoter, forward primer"
protein_bind 2817..2841
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 2850..2876
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 2880..3443
/codon_start=1
/product="human oncoprotein generated by the G12V mutation
in the small GTPase K-Ras"
/label=K-Ras (G12V)
/translation="TEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVI
DGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKD
SEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIR
KHKEKMSKDGKKKKKKSKTKCVIM"
protein_bind complement(3445..3469)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
misc_feature 3500..4088
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3553..3573)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3971..3982)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
promoter 4144..4643
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(4179..4202)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 4576..4595
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 4661..5686
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
LTR 6060..6293
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 6365..6486
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6402..6421)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6456..6475
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 6526..6661
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6588..6607
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(6681..6700)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(6682..6700)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(6710..6727)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(6710..6726)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6719..6741)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 6868..7323
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6955..6974)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7165..7186
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 7349..7453
/gene="bla"
/label=AmpR promoter
CDS 7454..8314
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7672..7691)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 8485..9073
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8974..8993
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 9227..9244
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
241 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
301 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
361 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
421 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
481 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
541 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
601 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
661 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
721 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
781 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
841 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
901 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
961 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
1021 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
1081 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
1141 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
1201 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
1261 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
1321 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
1381 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
1441 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
1501 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
1561 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
1621 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
1681 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
1741 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
1801 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
1861 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
1921 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
1981 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
2041 agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa
2101 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
2161 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg atcacgagac
2221 tagcctcgag aagcttgata tcgaattcca cggggttggg gttgcgcctt ttccaaggca
2281 gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg
2341 ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg
2401 ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct
2461 tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg
2521 cagacggaca gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata
2581 gcggctgctc agcagggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg
2641 ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc
2701 ctccggagcg cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc
2761 agggggatcc accggttcgt cgactagtcc agtgtggtgg aattctgcag atatcaacaa
2821 gtttgtacaa aaaagcaggc ttcaccatgt acccatacga tgttccagat tacgctgata
2881 ctgaatataa acttgtggta gttggagctg ttggcgtagg caagagtgcc ttgacgatac
2941 agctaattca gaatcatttt gtggacgaat atgatccaac aatagaggat tcctacagga
3001 agcaagtagt aattgatgga gaaacctgtc tcttggatat tctcgacaca gcaggtcaag
3061 aggagtacag tgcaatgagg gaccagtaca tgaggactgg ggagggcttt ctttgtgtat
3121 ttgccataaa taatactaaa tcatttgaag atattcacca ttatagagaa caaattaaaa
3181 gagttaagga ctctgaagat gtacctatgg tcctagtagg aaataaatgt gatttgcctt
3241 ctagaacagt agacacaaaa caggctcagg acttagcaag aagttatgga attcctttta
3301 ttgaaacatc agcaaagaca agacagggtg ttgatgatgc cttctataca ttagttcgag
3361 aaattcgaaa acataaagaa aagatgagca aagacggtaa aaagaagaaa aagaagtcaa
3421 agacaaagtg tgtaattatg taagacccag ctttcttgta caaagtggtt gatatccagc
3481 acagtggcgg ccgctcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
3541 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
3601 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
3661 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
3721 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
3781 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
3841 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
3901 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
3961 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
4021 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
4081 ctccccgcct ggaattctgc agatatccgg actagtgatc tctcgaggtt aacgaattct
4141 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc
4201 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta
4261 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag
4321 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag
4381 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc
4441 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa
4501 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt
4561 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct
4621 cttcctcatc tccgggcctt tcgacctgca tcccgccacc atgaaaaagc ctgaactcac
4681 cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
4741 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
4801 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt
4861 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct
4921 gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga
4981 actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct
5041 tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg
5101 gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga
5161 cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga
5221 ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga
5281 caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata
5341 cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg
5401 ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct
5461 ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc
5521 ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac
5581 acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga
5641 tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatagagta gatgccgacc
5701 gaacaagagc tgatttcgag aacgcctcag ccagcaactc gcgcgagcct agcaaggcaa
5761 atgcgagaga acggccttac gcttggtggc acagttctcg tccacagttc gctaagctcg
5821 ctcggctggg tcgcgggagg gccggtcgca gtgattcagg cccttctgga ttgtgttggt
5881 ccccagggca cgattgtcat gcccacgcac tcgggtgatc tgactgatcc cgcagattgg
5941 agatcgccgc ccgtgcctgc cgattgggtg cagatccgtc gagggcccgg tacctttaag
6001 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
6061 ggaagggcta gctcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
6121 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
6181 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
6241 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
6301 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
6361 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
6421 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
6481 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
6541 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
6601 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
6661 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
6721 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
6781 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
6841 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
6901 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
6961 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
7021 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
7081 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
7141 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
7201 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
7261 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
7321 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
7381 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
7441 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
7501 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
7561 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
7621 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
7681 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
7741 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
7801 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
7861 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
7921 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
7981 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
8041 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
8101 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
8161 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
8221 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
8281 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
8341 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
8401 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
8461 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
8521 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
8581 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
8641 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
8701 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
8761 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
8821 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
8881 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
8941 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
9001 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
9061 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
9121 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
9181 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
9241 cgaggaagcg gaag
//
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