| 质粒名称: | pCXLE-hOCT3/4-shp53-F |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 27077 |
| 存储实验室: | pCXLE-hOCT3/4-shp53-F |
|
载体骨架基本信息 |
|
| 载体名称: | pCXLE |
| 载体抗性: | 氨苄西林 |
| 空载体大小: | 10180 bp |
| 载体类型: | 哺乳细胞表达 |
| 生长说明： | DH5alpha |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | OCT3/4 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1108 bp |
| 克隆方法: | 限制性酶 |
| 5' 克隆位点: | Ecori（未销毁） |
| 3' 克隆位点: | Ecori（未销毁） |
| 5' 测序引物 | PCAG-F |
| 3' 测序引物 | WPRE-R |
|
文献引用方法 |
|
| 材料和方法部分: | pCXLE-hOCT3/4-shp53-F was a gift from Shinya Yamanaka (Addgene plasmid # 27077 ; http://n2t.net/addgene:27077 ; RRID:Addgene_27077) |
| 参考文献部分: | A more efficient method to generate integration-free human iPS cells. Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi M, Takahashi J, Saji H, Yamanaka S. Nat Methods. 2011 Apr 3. ():. 10.1038/nmeth.1591 PubMed 21460823 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCXLE-hOCT3/4-shp53-F 11681 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Integration-free (episomal) expression of human OCT3/4 and shRNA
against p53.
ACCESSION .
VERSION .
KEYWORDS pCXLE-hOCT3/4-shp53-F
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11681)
AUTHORS Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong
H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi
M, Takahashi J, Saji H, Yamanaka S
TITLE A more efficient method to generate integration-free human iPS
cells.
JOURNAL Nat Methods. 2011 Apr 3. ():.
PUBMED 21460823
REFERENCE 2 (bases 1 to 11681)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11681
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 4..383
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 385..661
/label=chicken beta-actin promoter
intron 662..1678
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 1602..1624
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 1686..1705
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
misc_feature 2866..3454
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(2919..2939)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3337..3348)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(3544..3563)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_site 3609..3664
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal"
primer_bind complement(3663..3682)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(4025..4041)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4025..4041)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4038..4060)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4049..4065
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4073..4103)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4118..4139
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
protein_bind complement(4228..4261)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
CDS 4722..6647
/codon_start=1
/product="Epstein-Barr nuclear antigen 1, also known as
EBNA-1"
/label=EBNA1
/note="crucial for latent viral infection, and for episomal
amplification of vectors containing the oriP origin (Okita
et al., 2013)"
/translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
GEEGQE"
protein_bind complement(6815..6848)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
rep_origin 7035..8824
/label=oriP
/note="Epstein-Barr virus oriP replication origin (Yates et
al., 2000)"
promoter 9295..9608
/label=U6 promoter
/note="RNA polymerase III promoter for mouse U6 snRNA (Das
et al., 1988)"
primer_bind 9527..9547
/label=mU6-F
/note="Mouse U6 promoter, forward primer"
primer_bind complement(9759..9776)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(9930..10518)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(10010..10029)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(10689..11549)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 11312..11331
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(11550..11654)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
481 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
541 ggggcgggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcgcccttca
1741 ccatggcggg acacctggct tcggatttcg ccttctcgcc ccctccaggt ggtggaggtg
1801 atgggccagg ggggccggag ccgggctggg ttgatcctcg gacctggcta agcttccaag
1861 gccctcctgg agggccagga atcgggccgg gggttgggcc aggctctgag gtgtggggga
1921 ttcccccatg ccccccgccg tatgagttct gtggggggat ggcgtactgt gggccccagg
1981 ttggagtggg gctagtgccc caaggcggct tggagacctc tcagcctgag ggcgaagcag
2041 gagtcggggt ggagagcaac tccgatgggg cctccccgga gccctgcacc gtcacccctg
2101 gtgccgtgaa gctggagaag gagaagctgg agcaaaaccc ggaggagtcc caggacatca
2161 aagctctgca gaaagaactc gagcaatttg ccaagctcct gaagcagaag aggatcaccc
2221 tgggatatac acaggccgat gtggggctca ccctgggggt tctatttggg aaggtattca
2281 gccaaacgac catctgccgc tttgaggctc tgcagcttag cttcaagaac atgtgtaagc
2341 tgcggccctt gctgcagaag tgggtggagg aagctgacaa caatgaaaat cttcaggaga
2401 tatgcaaagc agaaaccctc gtgcaggccc gaaagagaaa gcgaaccagt atcgagaacc
2461 gagtgagagg caacctggag aatttgttcc tgcagtgccc gaaacccaca ctgcagcaga
2521 tcagccacat cgcccagcag cttgggctcg agaaggatgt ggtccgagtg tggttctgta
2581 accggcgcca gaagggcaag cgatcaagca gcgactatgc acaacgagag gattttgagg
2641 ctgctgggtc tcctttctca gggggaccag tgtcctttcc tctggcccca gggccccatt
2701 ttggtacccc aggctatggg agccctcact tcactgcact gtactcctcg gtccctttcc
2761 ctgaggggga agcctttccc cctgtctctg tcaccactct gggctctccc atgcattcaa
2821 actgaggtaa gggcgaattc aagcttcggg gactagtcat atgataatca acctctggat
2881 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
2941 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
3001 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
3061 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
3121 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
3181 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
3241 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
3301 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
3361 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
3421 acgagtcgga tctccctttg ggccgcctcc ccgcatcggt aaattcactc ctcaggtgca
3481 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg
3541 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
3601 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
3661 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt
3721 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag
3781 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt
3841 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct
3901 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt
3961 catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat
4021 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
4081 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
4141 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag cggatctcaa
4201 ttccgatcat attcaataac ccttaatata acttcgtata atgtatgcta tacgaagtta
4261 ttaggtctga agaggagttt acgtccagcc aagcttagga tcaattctca tgtttgacag
4321 cttatcatcg ataagctgat cctcacaggc cgcacccagc ttttcttccg ttgccccagt
4381 agcatctctg tctggtgacc ttgaagagga agaggagggg tcccgagaat ccccatccct
4441 accgtccagc aaaaaggggg acgaggaatt tgaggcctgg cttgaggctc aggacgcaaa
4501 tcttgaggat gttcagcggg agttttccgg gctgcgagta attggtgatg aggacgagga
4561 tggttcggag gatggggaat tttcagacct ggatctgtct gacagcgacc atgaagggga
4621 tgagggtggg ggggctgttg gagggggcag gagtctgcac tccctgtatt cactgagcgt
4681 cgtctaataa agatgtctat tgatctcttt tagtgtgaat catgtctgac gaggggccag
4741 gtacaggacc tggaaatggc ctaggagaga agggagacac atctggacca gaaggctccg
4801 gcggcagtgg acctcaaaga agagggggtg ataaccatgg acgaggacgg ggaagaggac
4861 gaggacgagg aggcggaaga ccaggagccc cgggcggctc aggatcaggg ccaagacata
4921 gagatggtgt ccggagaccc caaaaacgtc caagttgcat tggctgcaaa gggacccacg
4981 gtggaacagg agcaggagca ggagcgggag gggcaggagc aggaggggca ggagcaggag
5041 gaggggcagg agcaggagga ggggcaggag gggcaggagg ggcaggaggg gcaggagcag
5101 gaggaggggc aggagcagga ggaggggcag gaggggcagg aggggcagga gcaggaggag
5161 gggcaggagc aggaggaggg gcaggagggg caggagcagg aggaggggca ggaggggcag
5221 gaggggcagg agcaggagga ggggcaggag caggaggagg ggcaggaggg gcaggagcag
5281 gaggaggggc aggaggggca ggaggggcag gagcaggagg aggggcagga gcaggagggg
5341 caggaggggc aggaggggca ggagcaggag gggcaggagc aggaggaggg gcaggagggg
5401 caggaggggc aggagcagga ggggcaggag caggaggggc aggagcagga ggggcaggag
5461 caggaggggc aggaggggca ggagcaggag gggcaggagg ggcaggagca ggaggggcag
5521 gaggggcagg agcaggagga ggggcaggag gggcaggagc aggaggaggg gcaggagggg
5581 caggagcagg aggggcagga ggggcaggag caggaggggc aggaggggca ggagcaggag
5641 gggcaggagg ggcaggagca ggaggagggg caggagcagg aggggcagga gcaggaggtg
5701 gaggccgggg tcgaggaggc agtggaggcc ggggtcgagg aggtagtgga ggccggggtc
5761 gaggaggtag tggaggccgc cggggtagag gacgtgaaag agccaggggg ggaagtcgtg
5821 aaagagccag ggggagaggt cgtggacgtg gagaaaagag gcccaggagt cccagtagtc
5881 agtcatcatc atccgggtct ccaccgcgca ggccccctcc aggtagaagg ccatttttcc
5941 accctgtagg ggaagccgat tattttgaat accaccaaga aggtggccca gatggtgagc
6001 ctgacgtgcc cccgggagcg atagagcagg gccccgcaga tgacccagga gaaggcccaa
6061 gcactggacc ccggggtcag ggtgatggag gcaggcgcaa aaaaggaggg tggtttggaa
6121 agcatcgtgg tcaaggaggt tccaacccga aatttgagaa cattgcagaa ggtttaagag
6181 ctctcctggc taggagtcac gtagaaagga ctaccgacga aggaacttgg gtcgccggtg
6241 tgttcgtata tggaggtagt aagacctccc tttacaacct aaggcgagga actgcccttg
6301 ctattccaca atgtcgtctt acaccattga gtcgtctccc ctttggaatg gcccctggac
6361 ccggcccaca acctggcccg ctaagggagt ccattgtctg ttatttcatg gtctttttac
6421 aaactcatat atttgctgag gttttgaagg atgcgattaa ggaccttgtt atgacaaagc
6481 ccgctcctac ctgcaatatc agggtgactg tgtgcagctt tgacgatgga gtagatttgc
6541 ctccctggtt tccacctatg gtggaagggg ctgccgcgga gggtgatgac ggagatgacg
6601 gagatgaagg aggtgatgga gatgagggtg aggaagggca ggagtgatgt aacttgttag
6661 gagacgccct caatcgtatt aaaagccgtg tattcccccg cactaaagaa taaatcccca
6721 gtagacatca tgcgtgctgt tggtgtattt ctggccatct gtcttgtcac cattttcgtc
6781 ctcccaacat ggggcaattg ccggaaccct taatataact tcgtataatg tatgctatac
6841 gaagttatta ggtccctcga agaggttcac tagcggatct caattgggca tacccatgtt
6901 gtcacgtcac tcagctccgc gctcaacacc ttctcgcgtt ggaaaacatt agcgacattt
6961 acctggtgag caatcagaca tgcgacggct ttagcctggc ctccttaaat tcacctaaga
7021 atgggagcaa ccagcaggaa aaggacaagc agcgaaaatt cacgccccct tgggaggtgg
7081 cggcatatgc aaaggatagc actcccactc tactactggg tatcatatgc tgactgtata
7141 tgcatgagga tagcatatgc tacccggata cagattagga tagcatatac tacccagata
7201 tagattagga tagcatatgc tacccagata tagattagga tagcctatgc tacccagata
7261 taaattagga tagcatatac tacccagata tagattagga tagcatatgc tacccagata
7321 tagattagga tagcctatgc tacccagata tagattagga tagcatatgc tacccagata
7381 tagattagga tagcatatgc tatccagata tttgggtagt atatgctacc cagatataaa
7441 ttaggatagc atatactacc ctaatctcta ttaggatagc atatgctacc cggatacaga
7501 ttaggatagc atatactacc cagatataga ttaggatagc atatgctacc cagatataga
7561 ttaggatagc ctatgctacc cagatataaa ttaggatagc atatactacc cagatataga
7621 ttaggatagc atatgctacc cagatataga ttaggatagc ctatgctacc cagatataga
7681 ttaggatagc atatgctatc cagatatttg ggtagtatat gctacccatg gcaacattag
7741 cccaccgtgc tctcagcgac ctcgtgaata tgaggaccaa caaccctgtg cttggcgctc
7801 aggcgcaagt gtgtgtaatt tgtcctccag atcgcagcaa tcgcgcccct atcttggccc
7861 gcccacctac ttatgcaggt attccccggg gtgccattag tggttttgtg ggcaagtggt
7921 ttgaccgcag tggttagcgg ggttacaatc agccaagtta ttacaccctt attttacagt
7981 ccaaaaccgc agggcggcgt gtgggggctg acgcgtgccc ccactccaca atttcaaaaa
8041 aaagagtggc cacttgtctt tgtttatggg ccccattggc gtggagcccc gtttaatttt
8101 cgggggtgtt agagacaacc agtggagtcc gctgctgtcg gcgtccactc tctttcccct
8161 tgttacaaat agagtgtaac aacatggttc acctgtcttg gtccctgcct gggacacatc
8221 ttaataaccc cagtatcata ttgcactagg attatgtgtt gcccatagcc ataaattcgt
8281 gtgagatgga catccagtct ttacggcttg tccccacccc atggatttct attgttaaag
8341 atattcagaa tgtttcattc ctacactagt atttattgcc caaggggttt gtgagggtta
8401 tattggtgtc atagcacaat gccaccactg aaccccccgt ccaaatttta ttctgggggc
8461 gtcacctgaa accttgtttt cgagcacctc acatacacct tactgttcac aactcagcag
8521 ttattctatt agctaaacga aggagaatga agaagcaggc gaagattcag gagagttcac
8581 tgcccgctcc ttgatcttca gccactgccc ttgtgactaa aatggttcac taccctcgtg
8641 gaatcctgac cccatgtaaa taaaaccgtg acagctcatg gggtgggaga tatcgctgtt
8701 ccttaggacc cttttactaa ccctaattcg atagcatatg cttcccgttg ggtaacatat
8761 gctattgaat tagggttagt ctggatagta tatactacta cccgggaagc atatgctacc
8821 cgtttagggt taacaagggg gccttataaa cactattgct aatgccctct tgagggtccg
8881 cttatcggta gctacacagg cccctctgat tgacgttggt gtagcctccc gtagtcttcc
8941 tgggcccctg ggaggtacat gtcccccagc attggtgtaa gagcttcagc caagagttac
9001 acataaaggc aatgttgtgt tgcagtccac agactgcaaa gtctgctcca ggatgaaagc
9061 cactcagtgt tggcaaatgt gcacatccat ttataaggat gtcaactaca gtcagagaac
9121 ccctttgtgt ttggtccccc cccgtgtcac atgtggaaca gggcccagtt ggcaagttgt
9181 accaaccaac tgaagggatt acatgcactg ccccgcgaag aaggggcaga gatgtcgtag
9241 tcaggtttag ttcgtccggg gcggggcatc gatcctctag agtcgacgct agcggatccg
9301 acgccgccat ctctaggccc gcgccggccc cctcgcacag acttgtggga gaagctcggc
9361 tactcccctg ccccggttaa tttgcatata atatttccta gtaactatag aggcttaatg
9421 tgcgataaaa gacagataat ctgttctttt taatactagc tacattttac atgataggct
9481 tggatttcta taagagatac aaatactaaa ttattatttt aaaaaacagc acaaaaggaa
9541 actcacccta actgtaaagt aattgtgtgt tttgagacta taaatatccc ttggagaaaa
9601 gccttgtttg ggccgactcc agtggtaatc tacttcaaga gagtagatta ccactggagt
9661 cttttttgga attcctgcag cccgggggat ccgctgcatt aatgaatcgg ccaacgcgcg
9721 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
9781 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
9841 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
9901 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
9961 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
10021 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
10081 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
10141 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
10201 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
10261 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
10321 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
10381 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
10441 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
10501 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
10561 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
10621 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
10681 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
10741 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
10801 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
10861 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
10921 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
10981 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
11041 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
11101 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
11161 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
11221 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
11281 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
11341 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
11401 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
11461 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
11521 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
11581 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
11641 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg g
//
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