pLenti CMV V5-LUC Blast (w567-1)

价格:1200元

联系方式:I47-825O-882O

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载体基本信息

质粒名称: pLenti CMV V5-LUC Blast (w567-1) 
出品公司: Addgene
目录编号: 21474
存储实验室: Eric Campeau
用途: 第三代慢病毒V5荧光素酶表达载体,CMV启动子,BLAST

载体骨架基本信息
载体名称: p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
载体抗性: 氨苄霉素
空载体大小: 7687 bp
载体类型: 哺乳细胞表达,慢病毒,荧光素酶
筛选标记: 杀稻瘟菌素
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: V5-LUC
物种来源: 萤火虫
插入大小: 1776 bp
标签/融合蛋白: V5(N端)
克隆方法: 限制性内切酶
5' 克隆位点: Bam HI(未破坏)
3' 克隆位点: Xba I (未破坏)
5' 测序引物 CMVforw
3' 测序引物 n/a

文献引用方法
材料和方法部分: pLenti CMV V5-LUC Blast (w567-1) was a gift from Eric Campeau (Addgene plasmid # 21474)
参考文献部分:
A Versatile Viral System for Expression and Depletion of Proteins in Mammalian Cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394

载体质粒图谱和多克隆位点信息

pLenti CMV V5-LUC Blast (w567-1)载体图谱

载体简介

载体序列

LOCUS       pLenti CMV V5-LUC Blast (w567-1)	9463 bp 	DNA	SYN
DEFINITION  pLenti CMV V5-LUC Blast (w567-1)
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..9463
                     /organism="pLenti CMV V5-LUC Blast (w567-1)"
                     /mol_type="other DNA"
     promoter        62..90
                     /label="AmpR_promoter"
     gene            132..992
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             132..992
                     /label="ORF frame 3"
     rep_origin      1147..1766
                     /label="pBR322_origin"
     promoter        2075..2104
                     /label="lac_promoter"
     misc_feature    2118..2140
                     /label="M13_pUC_rev_primer"
     promoter        2139..2157
                     /label="M13_reverse_primer"
     promoter        2174..2193
                     /label="T3_promoter"
     misc_feature    2448..2628
                     /label="HIV-1_5_LTR"
     misc_feature    2448..2628
                     /label="truncHIV-1_3_LTR"
     misc_feature    2736..2780
                     /label="HIV-1_psi_pack"
     CDS             3168..3668
                     /label="ORF frame 3"
     misc_feature    3290..3523
                     /label="RRE"
     CDS             3629..4171
                     /label="ORF frame 2"
     misc_feature    4016..4031
                     /label="cPPT"
     promoter        4149..4653
                     /label="CMV_immearly_promoter"
     misc_feature    4156..4443
                     /label="CAG_enhancer"
     misc_feature    4610..4630
                     /label="CMV_fwd_primer"
     promoter        4611..4680
                     /label="CMV_promoter"
     misc_feature    4654..4673
                     /label="pCEP_fwd_primer"
     misc_feature    4656..4680
                     /label="LNCX_primer"
     misc_feature    4764..4788
                     /label="attB1"
     CDS             4794..6569
                     /label="ORF frame 3"
     misc_feature    4800..4841
                     /label="V5"
     gene            4917..6569
                     /label="firefly_Luciferase"
                     /gene="firefly_Luciferase"
     misc_feature    complement(4983..5001)
                     /label="LucNrev_primer"
     misc_feature    6576..6600
                     /label="attB2"
     misc_feature    6633..7208
                     /label="WPRE"
     CDS             complement(6718..7341)
                     /label="ORF frame 2"
     CDS             6721..7245
                     /label="ORF frame 1"
     CDS             7041..7553
                     /label="ORF frame 3"
     misc_feature    complement(7270..7290)
                     /label="pBABE_3_primer"
     misc_feature    complement(7276..7492)
                     /label="SV40_enhancer"
     promoter        7288..7557
                     /label="SV40_promoter"
     rep_origin      7456..7533
                     /label="SV40_origin"
     misc_feature    7518..7537
                     /label="SV40pro_F_primer"
     promoter        7651..7718
                     /label="EM7_promoter"
     gene            7719..8117
                     /label="blast"
                     /gene="blast"
     misc_feature    8187..8202
                     /label="cPPT"
     misc_feature    8187..8208
                     /label="U3PPT"
     misc_feature    8257..8437
                     /label="HIV-1_5_LTR"
     misc_feature    8257..8437
                     /label="truncHIV-1_3_LTR"
     terminator      8512..8631
                     /label="SV40_PA_terminator"
     misc_feature    8600..8619
                     /label="EBV_rev_primer"
     rep_origin      8649..8726
                     /label="SV40_origin"
     misc_feature    8711..8730
                     /label="SV40pro_F_primer"
     promoter        complement(8817..8835)
                     /label="T7_promoter"
     promoter        complement(8845..8861)
                     /label="M13_forward20_primer"
     misc_feature    complement(8854..8876)
                     /label="M13_pUC_fwd_primer"
     misc_feature    8854..8997
                     /label="lacZ_a"
     rep_origin      9023..9329
                     /label="f1_origin"
ORIGIN
    1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
   61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
  121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
  181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
  241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
  301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
  361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
  421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
  481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
  541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
  601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
  661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
  721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
  781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
  841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
  901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
  961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
 2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
 2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
 2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
 2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
 2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
 2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
 2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
 2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
 2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
 2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
 2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
 2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
 2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
 2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
 3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
 3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
 3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
 3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
 3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
 3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
 3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
 3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
 3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
 3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
 3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
 3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
 3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
 3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
 3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
 3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
 3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggttaa cttttaaaag
 4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
 4081 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgataagct
 4141 tgggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
 4201 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
 4261 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
 4321 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
 4381 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
 4441 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
 4501 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
 4561 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
 4621 ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc
 4681 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccgact CTAGTCCAGT
 4741 GTGGTGGAAT TCTGCAGATA TCAACAAGTT TGTACAAAAA AGCAGGCTCC ACcatggaag
 4801 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gggatcCATG GGAACCAATT
 4861 CAGTCCCTAG GCTTTTGCAA AAAGCTTGGC ATTCCGGTAC TGTTGGTAAA GCCACCATGG
 4921 AAGACGCCAA AAACATAAAG AAAGGCCCGG CGCCATTCTA TCCGCTGGAA GATGGAACCG
 4981 CTGGAGAGCA ACTGCATAAG GCTATGAAGA GATACGCCCT GGTTCCTGGA ACAATTGCTT
 5041 TTACAGATGC ACATATCGAG GTGGACATCA CTTACGCTGA GTACTTCGAA ATGTCCGTTC
 5101 GGTTGGCAGA AGCTATGAAA CGATATGGGC TGAATACAAA TCACAGAATC GTCGTATGCA
 5161 GTGAAAACTC TCTTCAATTC TTTATGCCGG TGTTGGGCGC GTTATTTATC GGAGTTGCAG
 5221 TTGCGCCCGC GAACGACATT TATAATGAAC GTGAATTGCT CAACAGTATG GGCATTTCGC
 5281 AGCCTACCGT GGTGTTCGTT TCCAAAAAGG GGTTGCAAAA AATTTTGAAC GTGCAAAAAA
 5341 AGCTCCCAAT CATCCAAAAA ATTATTATCA TGGATTCTAA AACGGATTAC CAGGGATTTC
 5401 AGTCGATGTA CACGTTCGTC ACATCTCATC TACCTCCCGG TTTTAATGAA TACGATTTTG
 5461 TGCCAGAGTC CTTCGATAGG GACAAGACAA TTGCACTGAT CATGAACTCC TCTGGATCTA
 5521 CTGGTCTGCC TAAAGGTGTC GCTCTGCCTC ATAGAACTGC CTGCGTGAGA TTCTCGCATG
 5581 CCAGAGATCC TATTTTTGGC AATCAAATCA TTCCGGATAC TGCGATTTTA AGTGTTGTTC
 5641 CATTCCATCA CGGTTTTGGA ATGTTTACTA CACTCGGATA TTTGATATGT GGATTTCGAG
 5701 TCGTCTTAAT GTATAGATTT GAAGAAGAGC TGTTTCTGAG GAGCCTTCAG GATTACAAGA
 5761 TTCAAAGTGC GCTGCTGGTG CCAACCCTAT TCTCCTTCTT CGCCAAAAGC ACTCTGATTG
 5821 ACAAATACGA TTTATCTAAT TTACACGAAA TTGCTTCTGG TGGCGCTCCC CTCTCTAAGG
 5881 AAGTCGGGGA AGCGGTTGCC AAGAGGTTCC ATCTGCCAGG TATCAGGCAA GGATATGGGC
 5941 TCACTGAGAC TACATCAGCT ATTCTGATTA CACCCGAGGG GGATGATAAA CCGGGCGCGG
 6001 TCGGTAAAGT TGTTCCATTT TTTGAAGCGA AGGTTGTGGA TCTGGATACC GGGAAAACGC
 6061 TGGGCGTTAA TCAAAGAGGC GAACTGTGTG TGAGAGGTCC TATGATTATG TCCGGTTATG
 6121 TAAACAATCC GGAAGCGACC AACGCCTTGA TTGACAAGGA TGGATGGCTA CATTCTGGAG
 6181 ACATAGCTTA CTGGGACGAA GACGAACACT TCTTCATCGT TGACCGCCTG AAGTCTCTGA
 6241 TTAAGTACAA AGGCTATCAG GTGGCTCCCG CTGAATTGGA ATCCATCTTG CTCCAACACC
 6301 CCAACATCTT CGACGCAGGT GTCGCAGGTC TTCCCGACGA TGACGCCGGT GAACTTCCCG
 6361 CCGCCGTTGT TGTTTTGGAG CACGGAAAGA CGATGACGGA AAAAGAGATC GTGGATTACG
 6421 TCGCCAGTCA AGTAACAACC GCGAAAAAGT TGCGCGGAGG AGTTGTGTTT GTGGACGAAG
 6481 TACCGAAAGG TCTTACCGGA AAACTCGACG CAAGAAAAAT CAGAGAGATC CTCATAAAGG
 6541 CCAAGAAGGG CGGAAAGATC GCCGTGTAAT TCTAGACCCA GCTTTCTTGT ACAAAGTGGT
 6601 TGATATCCAG CACAGTGGCG GCCGCTCGAc aatcaacctc tggattacaa aatttgtgaa
 6661 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
 6721 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
 6781 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
 6841 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
 6901 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
 6961 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
 7021 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
 7081 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
 7141 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
 7201 ctttgggccg cctccccgcc tggAATTCTG CAGATATCCG GTTAGTAATG AGTTTGGAAT
 7261 TAATTCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGGCAGGC
 7321 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC
 7381 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG
 7441 CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT
 7501 GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCTGCCTC TGAGCTATTC
 7561 CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT
 7621 TGTATATCCA TTTTCGGATC TGATCAGCAC GTGTTGACAA TTAATCATCG GCATAGTATA
 7681 TCGGCATAGT ATAATACGAC AAGGTGAGGA ACTAAACCAT GGCCAAGCCT TTGTCTCAAG
 7741 AAGAATCCAC CCTCATTGAA AGAGCAACGG CTACAATCAA CAGCATCCCC ATCTCTGAAG
 7801 ACTACAGCGT CGCCAGCGCA GCTCTCTCTA GCGACGGCCG CATCTTCACT GGTGTCAATG
 7861 TATATCATTT TACTGGGGGA CCTTGTGCAG AACTCGTGGT GCTGGGCACT GCTGCTGCTG
 7921 CGGCAGCTGG CAACCTGACT TGTATCGTCG CGATCGGAAA TGAGAACAGG GGCATCTTGA
 7981 GCCCCTGCGG ACGGTGCCGA CAGGTGCTTC TCGATCTGCA TCCTGGGATC AAAGCCATAG
 8041 TGAAGGACAG TGATGGACAG CCGACGGCAG TTGGGATTCG TGAATTGCTG CCCTCTGGTT
 8101 ATGTGTGGGA GGGCTAAGCA CAATTCGAGC TCGGTACCtt taagaccaat gacttacaag
 8161 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctagctcac
 8221 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
 8281 tgagcctggg agctctctgg ctgcctaggg aacccactgc ttaagcctca ataaagcttg
 8341 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
 8401 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
 8461 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
 8521 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
 8581 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
 8641 ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
 8701 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
 8761 agtgaggagg cttttttgga ggcctaggct tttgcgtcga gacgtaccca attcgcccta
 8821 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
 8881 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
 8941 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
 9001 gcgcgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
 9061 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
 9121 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
 9181 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
 9241 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
 9301 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
 9361 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
 9421 atttaacgcg aattttaaca aaatattaac gtttacaatt tcc
//

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