pLVX-mCherry-N1

价格:2000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pLVX-mCherry-N1
质粒类型: 哺乳动物细胞慢病毒表达载体
高拷贝/低拷贝: 高拷贝
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 8778 bp
5' 测序引物及序列: CMV-F: CGCAAATGGGCGGTAGGCGTG (Invitrogen)
3' 测序引物及序列: --
载体标签: C-mCherry
载体抗性: 氨苄青霉素
筛选标记: 嘌呤霉素(Puromycin)
备注: 该载体要用Stbl3感受态细胞进行质粒扩增
产品目录号: 632562
稳定性: 稳表达
组成型: 组成型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVX-mCherry-N1载体图谱



pLVX-ZsGreen1-C1多克隆位点

pLVX-ZsGreen1-C1载体特征

载体简介

载体序列

LOCUS       pLVX-mCherry-N1   8778 bp    DNA   circular             7-OCT-2009
COMMENT     Created by Clontech Laboratories Inc.
COMMENT     Cat. No. 632562
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=packaging\signal
     misc_feature    4746..5337
                     /label=WPRE
     CDS             complement(7464..8460)
                     /label=Amp(R)
     misc_binding    1303..1536
                     /label=RRE
     misc_binding    636..653
                     /label=PBS
     LTR             5540..6176
                     /label=3'\LTR
     promoter        3604..4112
                     /label=PGK\Promoter
     CDS             4133..4732
                     /label=Puro(R)
     CDS             2875..3585
                     /label=mCherry
     misc_feature    2028..2151
                     /label=cPPT/CTS\(DNA\flap)
     promoter        2185..2787
                     /label=CMV\IE\Promoter
     rep_origin      complement(6646..7319)
                     /label=pUC\ori
     misc_feature    2815..2867
                     /label=MCS
BASE COUNT     2178 a      2271 c      2335 g      1994 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 
     2821 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccacc ggtcatggtg 
     2881 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac 
     2941 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc 
     3001 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc 
     3061 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc 
     3121 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg 
     3181 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag 
     3241 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag 
     3301 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg 
     3361 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc 
     3421 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc 
     3481 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc 
     3541 gagggccgcc actccaccgg cggcatggac gagctgtaca agtgagcggc cgcgactcta 
     3601 gataattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag 
     3661 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat 
     3721 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc 
     3781 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa 
     3841 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc 
     3901 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga 
     3961 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg 
     4021 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc 
     4081 tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga 
     4141 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct 
     4201 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat 
     4261 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa 
     4321 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga 
     4381 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct 
     4441 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg 
     4501 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt 
     4561 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc 
     4621 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga 
     4681 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaccgcgtct 
     4741 ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg 
     4801 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt 
     4861 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg 
     4921 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc 
     4981 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc 
     5041 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc 
     5101 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc 
     5161 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg 
     5221 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc 
     5281 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa 
     5341 ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc 
     5401 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt 
     5461 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt 
     5521 tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct 
     5581 tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg 
     5641 gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc 
     5701 agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct 
     5761 gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc 
     5821 atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc 
     5881 ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg 
     5941 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc 
     6001 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 
     6061 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 
     6121 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta 
     6181 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg 
     6241 agaggccttg acattgctag cgttttaccg tcgacctcta gctagagctt ggcgtaatca 
     6301 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 
     6361 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt 
     6421 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga 
     6481 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 
     6541 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 
     6601 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 
     6661 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 
     6721 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 
     6781 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 
     6841 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 
     6901 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 
     6961 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 
     7021 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 
     7081 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 
     7141 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 
     7201 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 
     7261 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 
     7321 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 
     7381 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 
     7441 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 
     7501 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 
     7561 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 
     7621 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 
     7681 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 
     7741 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 
     7801 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 
     7861 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 
     7921 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 
     7981 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 
     8041 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 
     8101 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 
     8161 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 
     8221 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 
     8281 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 
     8341 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 
     8401 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 
     8461 gacggatcgg gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag 
     8521 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 
     8581 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca 
     8641 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact 
     8701 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg 
     8761 tactacaaac ttagtagt 
//

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